##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4050235_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3931224
Sequences flagged as poor quality	0
Sequence length	25
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.706669983699733	31.0	31.0	34.0	27.0	34.0
2	30.705017826508996	31.0	31.0	34.0	27.0	34.0
3	30.890194249933355	31.0	31.0	34.0	27.0	34.0
4	34.53580742282811	37.0	35.0	37.0	31.0	37.0
5	34.476525886085355	37.0	35.0	37.0	31.0	37.0
6	34.44834433245218	37.0	35.0	37.0	31.0	37.0
7	34.393528326037895	37.0	35.0	37.0	30.0	37.0
8	34.36753972808469	37.0	35.0	37.0	30.0	37.0
9	35.953108751879824	39.0	37.0	39.0	31.0	39.0
10	35.84498517510068	39.0	37.0	39.0	30.0	39.0
11	35.83406465772492	39.0	37.0	39.0	30.0	39.0
12	35.7655066208387	39.0	37.0	39.0	30.0	39.0
13	35.759577169858545	39.0	37.0	39.0	30.0	39.0
14	36.91781236581787	40.0	37.0	41.0	31.0	41.0
15	36.88742361157746	40.0	37.0	41.0	31.0	41.0
16	36.87162344348732	40.0	37.0	41.0	31.0	41.0
17	36.85950915033079	40.0	37.0	41.0	31.0	41.0
18	36.828326241394535	40.0	37.0	41.0	31.0	41.0
19	36.88602989806737	40.0	37.0	41.0	31.0	41.0
20	36.833474765111326	40.0	37.0	41.0	31.0	41.0
21	36.78444550603069	40.0	37.0	41.0	30.0	41.0
22	36.72717046904476	40.0	37.0	41.0	30.0	41.0
23	36.63224888736943	40.0	37.0	41.0	30.0	41.0
24	36.5780512634233	40.0	37.0	41.0	30.0	41.0
25	36.359725622350695	40.0	37.0	41.0	30.0	41.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.830011621676011
1101	2	0.9996153269441024
1101	3	0.9059203602413071
1101	4	0.8476913823554
1101	5	0.9572307203561863
1101	6	1.0665640080146659
1101	7	0.9565765702659377
1101	8	0.9930560448940042
1101	9	1.1921503740984463
1101	10	1.1344235510876004
1101	11	1.1574281451238733
1101	12	1.277355386439993
1101	13	1.1887267502600452
1101	14	1.3888002908372243
1101	15	1.3915797751936907
1101	16	1.342010770605775
1101	17	1.4207227771385291
1101	18	1.452689307176506
1101	19	1.407645028833464
1101	20	1.3874048642264043
1101	21	1.491539158442599
1101	22	1.4454637133194765
1101	23	1.4905560291494382
1101	24	1.5660290675282127
1101	25	1.5198895390002178
1102	1	0.7710173722131728
1102	2	0.9342773388637404
1102	3	0.8750184288365972
1102	4	0.8227225610360804
1102	5	0.8528854394073164
1102	6	0.9165298620045306
1102	7	0.8803955334768716
1102	8	0.8780504014904693
1102	9	0.9677372725777005
1102	10	1.0059728264736805
1102	11	1.073472516275551
1102	12	1.1215371579028144
1102	13	1.0635857596124794
1102	14	1.2251750581634582
1102	15	1.239865943162144
1102	16	1.2579106851330906
1102	17	1.0946505172453485
1102	18	1.2299186442316596
1102	19	1.166239574674151
1102	20	1.1460241535527018
1102	21	1.172454307779546
1102	22	1.2203154469538546
1102	23	1.3265738858619116
1102	24	1.3238547898283315
1102	25	1.3792642261651125
1103	1	0.46205280931803827
1103	2	0.6865715834413493
1103	3	0.5889295812211017
1103	4	0.5034047845540996
1103	5	0.5559800758845554
1103	6	0.5629790938239481
1103	7	0.5589487330330272
1103	8	0.5789924060195304
1103	9	0.5806988051813491
1103	10	0.5851516482537846
1103	11	0.6693356913033952
1103	12	0.656710587322415
1103	13	0.601610850279144
1103	14	0.7332089723591224
1103	15	0.7427686315365847
1103	16	0.7075135628089981
1103	17	0.7703539611318106
1103	18	0.8279003272573533
1103	19	0.7795389943764377
1103	20	0.7210097523281576
1103	21	0.6601784372024113
1103	22	0.6673236693084803
1103	23	0.7663506891133096
1103	24	0.7975654444195683
1103	25	0.8395710142410593
1104	1	0.5661197299520531
1104	2	0.7482993555598689
1104	3	0.721736616955674
1104	4	0.5797057340162866
1104	5	0.6797703604857617
1104	6	0.7430124873907573
1104	7	0.6742891814624272
1104	8	0.699678344760791
1104	9	0.8044994848724869
1104	10	0.8719813991066943
1104	11	0.8122355942385866
1104	12	0.8411772624845639
1104	13	0.8532753178830035
1104	14	0.9469531280891772
1104	15	0.9641039731801868
1104	16	0.9980180043354281
1104	17	0.8648055554985135
1104	18	0.9902618700681103
1104	19	0.9363911395246092
1104	20	0.9981722674228095
1104	21	0.9898663655216708
1104	22	0.9700922722321366
1104	23	1.0113128961017068
1104	24	0.978254005678437
1104	25	1.0460108384110143
1105	1	0.4119085897609551
1105	2	0.5555070838428762
1105	3	0.4624736178277402
1105	4	0.44245107908363224
1105	5	0.45634253805213376
1105	6	0.570879884833829
1105	7	0.4604691143228479
1105	8	0.509091555025897
1105	9	0.53554797448394
1105	10	0.5890658466665926
1105	11	0.6274762104834579
1105	12	0.6908139362256733
1105	13	0.612509277618912
1105	14	0.6881591056936998
1105	15	0.6000427216063215
1105	16	0.6488533309511055
1105	17	0.6424970409975899
1105	18	0.7482915454291188
1105	19	0.6206402236813986
1105	20	0.666469654482249
1105	21	0.6003363702863993
1105	22	0.6553274358744616
1105	23	0.69887297488728
1105	24	0.677465431854003
1105	25	0.745229886639585
1106	1	0.335216111208819
1106	2	0.5567491392807113
1106	3	0.4000260931375834
1106	4	0.35656527389662074
1106	5	0.39625406176740086
1106	6	0.5547798834066384
1106	7	0.4901567978682664
1106	8	0.4990186436972337
1106	9	0.6038572726056657
1106	10	0.6753750223179722
1106	11	0.5743808945227826
1106	12	0.6008363529339462
1106	13	0.5570554621736648
1106	14	0.7168423555443013
1106	15	0.6820785788249353
1106	16	0.6107763678545695
1106	17	0.6088595668776833
1106	18	0.5601921619648422
1106	19	0.6290545706186563
1106	20	0.634546948151133
1106	21	0.6642536305906503
1106	22	0.6055533656030789
1106	23	0.7099585537237232
1106	24	0.715427731481121
1106	25	0.6399375332170081
1107	1	0.17375711773845026
1107	2	0.349563355240619
1107	3	0.35376348473362995
1107	4	0.30221212354827287
1107	5	0.28406844311221846
1107	6	0.31990351374882664
1107	7	0.28160780310115996
1107	8	0.2546639331691196
1107	9	0.2904985041465906
1107	10	0.2994553285464292
1107	11	0.38756628690799033
1107	12	0.4053387448680823
1107	13	0.3947105032081524
1107	14	0.37042349499071037
1107	15	0.4234286992631979
1107	16	0.3308827820667801
1107	17	0.44854600294412705
1107	18	0.4858420734326856
1107	19	0.43553394155885883
1107	20	0.4810484426160073
1107	21	0.38811188196575586
1107	22	0.3119197479150344
1107	23	0.39635235187743945
1107	24	0.4128340863011246
1107	25	0.44290752419841084
1108	1	0.24015093387326658
1108	2	0.416212377087426
1108	3	0.36616070715890814
1108	4	0.3492283424986411
1108	5	0.4322918478174458
1108	6	0.4902961221937474
1108	7	0.4343915815180708
1108	8	0.4073079814173397
1108	9	0.4848590236661039
1108	10	0.4760332865814476
1108	11	0.4749058205750103
1108	12	0.5054392643259717
1108	13	0.4112055249882971
1108	14	0.5391019231901524
1108	15	0.5805125762950496
1108	16	0.5621979401153538
1108	17	0.6022648447829582
1108	18	0.6238677525271896
1108	19	0.5688608051249133
1108	20	0.6626802411580925
1108	21	0.6647721824934507
1108	22	0.6370719344863147
1108	23	0.7176918009613544
1108	24	0.7006128359443764
1108	25	0.7305593431692756
1109	1	-0.007791521034370419
1109	2	0.10696694301441667
1109	3	0.13137731956303256
1109	4	0.1464897356330468
1109	5	0.22962479743352304
1109	6	0.21990179103988083
1109	7	0.25025862941354404
1109	8	0.2524749443418486
1109	9	0.2840943199873607
1109	10	0.2397448967781486
1109	11	0.22115503690765337
1109	12	0.21680804879249393
1109	13	0.2653976535221645
1109	14	0.30294137629934426
1109	15	0.37950487143674394
1109	16	0.2959260917898874
1109	17	0.22273804561480404
1109	18	0.20751640433116592
1109	19	0.22664894714672812
1109	20	0.2874379974215273
1109	21	0.3242713512182789
1109	22	0.2961047876371481
1109	23	0.2778485163890352
1109	24	0.3046486484418338
1109	25	0.3046076574485781
1110	1	-0.08793056407339961
1110	2	0.020507716396146236
1110	3	-0.06300733928601687
1110	4	-0.15113160299422645
1110	5	-0.11008129603521155
1110	6	-0.3380125143887369
1110	7	-0.2261776428018223
1110	8	-0.10538715609049376
1110	9	-0.15269749604470917
1110	10	-0.15394953044346948
1110	11	-0.15366079102340535
1110	12	-0.07937772741542659
1110	13	-0.17957436152071438
1110	14	-0.12680054087365988
1110	15	-0.15103077761771289
1110	16	-0.08145292448083552
1110	17	-0.15066681282888084
1110	18	-0.08364316640820135
1110	19	-0.14825275610375854
1110	20	-0.1221316922496598
1110	21	-0.15253189053004945
1110	22	-0.19429179738431657
1110	23	-0.1753032977795712
1110	24	-0.20321541642592678
1110	25	-0.16997374785553632
1111	1	-0.4038734245840523
1111	2	-0.25605306366317393
1111	3	-0.24468004862756487
1111	4	-0.28824719076752814
1111	5	-0.29754048858062276
1111	6	-0.4896677959218394
1111	7	-0.3976973558277308
1111	8	-0.3566159523800465
1111	9	-0.396686721952932
1111	10	-0.38038499212612464
1111	11	-0.44506407601657116
1111	12	-0.42691387168878236
1111	13	-0.43661707692587726
1111	14	-0.3889890601362609
1111	15	-0.37101602873667616
1111	16	-0.3535611424324543
1111	17	-0.5158317312952647
1111	18	-0.4312132129698085
1111	19	-0.542861442405794
1111	20	-0.45046343731775096
1111	21	-0.47542758381244
1111	22	-0.5182271916319081
1111	23	-0.5414464404459451
1111	24	-0.44915356202433543
1111	25	-0.5031765219680082
1112	1	-0.3675413297645669
1112	2	-0.2859395077612241
1112	3	-0.33149189423576786
1112	4	-0.2652126260477843
1112	5	-0.27438112744992793
1112	6	-1.147519049021767
1112	7	-0.3927930688457977
1112	8	-0.3290916314319432
1112	9	-0.3954573050807184
1112	10	-0.4196551901337813
1112	11	-0.434507827589222
1112	12	-0.43803355868914196
1112	13	-0.3205809235910948
1112	14	-0.4136721209709293
1112	15	-0.4291952676481401
1112	16	-0.43963736928142083
1112	17	-0.48003640235515377
1112	18	-0.5128850450420614
1112	19	-0.49713698245938787
1112	20	-0.5035029276538054
1112	21	-0.5028861503883206
1112	22	-0.636609438004399
1112	23	-0.5218473539945307
1112	24	-0.5251753756950848
1112	25	-0.478785535960661
1113	1	-0.6688461850966867
1113	2	-0.8057979152646269
1113	3	-0.6943769581140877
1113	4	-0.5939519338567649
1113	5	-0.6153390223216562
1113	6	-1.296713598170939
1113	7	-0.7580975117660387
1113	8	-0.6907145480438075
1113	9	-0.8342518248248965
1113	10	-0.779209471697456
1113	11	-0.8006875498063835
1113	12	-0.8636626385486679
1113	13	-0.7742044407798687
1113	14	-0.9941331556357866
1113	15	-1.0351114391170668
1113	16	-0.9774272209817525
1113	17	-1.0044226994225411
1113	18	-0.9421511812047498
1113	19	-1.002957410639361
1113	20	-0.969192063517724
1113	21	-0.9978181715572276
1113	22	-1.0342949575927776
1113	23	-0.9677144592408311
1113	24	-1.0817810556486265
1113	25	-1.0998374003793288
1114	1	-0.8842773823042904
1114	2	-0.846373253505039
1114	3	-0.9290869360744161
1114	4	-0.807201902220875
1114	5	-0.9841930912246326
1114	6	-1.5035032537615294
1114	7	-1.0644037972905096
1114	8	-1.1332054395432465
1114	9	-1.2440002272123536
1114	10	-1.3830101286694756
1114	11	-1.1320814272067565
1114	12	-1.217727257494353
1114	13	-1.286541779568637
1114	14	-1.4510503434158935
1114	15	-1.3720050186127892
1114	16	-1.3730057096297372
1114	17	-1.4129745401041873
1114	18	-1.5510221489466858
1114	19	-1.2946264939366685
1114	20	-1.3328670732721193
1114	21	-1.3737135332081962
1114	22	-1.2882731710085054
1114	23	-1.361584280181681
1114	24	-1.416148047813472
1114	25	-1.501438571961863
1115	1	-1.2886040035558786
1115	2	-1.9377468092386927
1115	3	-1.4776740595633626
1115	4	-1.1836086844288474
1115	5	-1.3390156050829063
1115	6	-1.7694405241816185
1115	7	-1.4069113942984544
1115	8	-1.4826481864877437
1115	9	-1.4444539605991125
1115	10	-1.5999608065563393
1115	11	-1.6169022713929593
1115	12	-1.5318071846966461
1115	13	-1.6290766757150337
1115	14	-1.776127816200237
1115	15	-1.777948967721187
1115	16	-1.8723261530946402
1115	17	-1.7988868489267347
1115	18	-1.9936004565670657
1115	19	-1.8466778530497336
1115	20	-1.8843522465840863
1115	21	-1.9894123744557746
1115	22	-2.080156895045107
1115	23	-1.9929528268991064
1115	24	-2.057160536960602
1115	25	-2.12171573752655
1116	1	-0.9350735888324451
1116	2	-1.05262620345162
1116	3	-1.0370687739708622
1116	4	-0.9380257146680577
1116	5	-0.9506845284067111
1116	6	-1.2163885207715595
1116	7	-1.216379324693115
1116	8	-1.2275195570308455
1116	9	-1.370600593165186
1116	10	-1.4113188126743879
1116	11	-1.430052063279021
1116	12	-1.358883058524441
1116	13	-1.3659223705146957
1116	14	-1.5434293557418854
1116	15	-1.5076932132454814
1116	16	-1.5344514226397976
1116	17	-1.5163077405570462
1116	18	-1.6113553334538864
1116	19	-1.4710958862476673
1116	20	-1.456721736579567
1116	21	-1.5608180211134197
1116	22	-1.5362022287796222
1116	23	-1.5778803631309941
1116	24	-1.7045528442671554
1116	25	-1.8834694108628867
1201	1	0.7057305884253786
1201	2	0.914810977343052
1201	3	0.8460101183593345
1201	4	0.7589504255634623
1201	5	0.7425948166345435
1201	6	0.8483875338119162
1201	7	0.83841051828324
1201	8	0.8237489549454224
1201	9	0.95224835367992
1201	10	0.9973723521994273
1201	11	0.9694645160701185
1201	12	1.0108249381383771
1201	13	1.0439378564593298
1201	14	1.2380134170494657
1201	15	1.2419766115525164
1201	16	1.1774121213469186
1201	17	1.2245923974613362
1201	18	1.2523310414492244
1201	19	1.2180533678950667
1201	20	1.195516144458395
1201	21	1.2074385841721522
1201	22	1.2023574724068808
1201	23	1.160118471141402
1201	24	1.273507194066056
1201	25	1.2517642691186666
1202	1	0.6847121595915979
1202	2	0.8535482973979356
1202	3	0.836984281657088
1202	4	0.8206526206727887
1202	5	0.8692171779669096
1202	6	0.9266930570724483
1202	7	0.9082580600804988
1202	8	0.9144923493895689
1202	9	1.0569479920558464
1202	10	1.063491182532914
1202	11	1.141646331400615
1202	12	1.1663046532391306
1202	13	1.1982336914477543
1202	14	1.303263996273408
1202	15	1.289751232458059
1202	16	1.3080406467456598
1202	17	1.228202874368037
1202	18	1.2200526330892174
1202	19	1.2632462910234779
1202	20	1.2814338547786406
1202	21	1.3626594108806032
1202	22	1.2922072022358648
1202	23	1.3399466451525797
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2211	4	-0.30110742262194634
2211	5	-0.30685880017464484
2211	6	-0.26435049343941586
2211	7	-0.27470234730679977
2211	8	-0.23483979760213458
2211	9	-0.27709805239310725
2211	10	-0.4238783073265324
2211	11	-0.30311691621889736
2211	12	-0.31167595677827364
2211	13	-0.2634397745817054
2211	14	-0.37982160024444767
2211	15	-0.4021953904166651
2211	16	-0.44587736934540345
2211	17	-0.41612758857500154
2211	18	-0.3685042642742502
2211	19	-0.4135738080573077
2211	20	-0.3794387377121069
2211	21	-0.40713850008586405
2211	22	-0.37109351628672727
2211	23	-0.33579985657286926
2211	24	-0.3988231724000073
2211	25	-0.39265661941486485
2212	1	-0.4074330483487323
2212	2	-0.45895552680076435
2212	3	-0.4589772474536318
2212	4	-0.4918352714465897
2212	5	-0.4518529369727631
2212	6	-0.42589479371203964
2212	7	-0.534355346844194
2212	8	-0.5114493928209924
2212	9	-0.48379286162436586
2212	10	-0.5294996676282508
2212	11	-0.5564888187151169
2212	12	-0.5905321574703777
2212	13	-0.5063825314576818
2212	14	-0.6880776038675478
2212	15	-0.6180659279160565
2212	16	-0.6666927314773048
2212	17	-0.604708244587286
2212	18	-0.5966642981456118
2212	19	-0.6334282928587314
2212	20	-0.610208504760017
2212	21	-0.6110730159002813
2212	22	-0.6135340481759641
2212	23	-0.6702947695338395
2212	24	-0.7375072933289744
2212	25	-0.5694814433797148
2213	1	-0.6752425436081353
2213	2	-0.9743109189983947
2213	3	-0.7602373231860611
2213	4	-0.6702757125108221
2213	5	-0.717893495608088
2213	6	-0.6512249590875356
2213	7	-0.7724745537485092
2213	8	-0.7364089253056605
2213	9	-0.7891600768090186
2213	10	-0.8639564228013157
2213	11	-0.8319110835484196
2213	12	-0.7789153307849688
2213	13	-0.8621993251868361
2213	14	-0.9618235087676297
2213	15	-1.03888288612211
2213	16	-1.0310614546531909
2213	17	-0.9632364395707569
2213	18	-1.0206314511181986
2213	19	-0.8646673209826332
2213	20	-0.9473284378195501
2213	21	-0.984765764212419
2213	22	-1.0331346382403979
2213	23	-1.021436179472488
2213	24	-1.0782169538892958
2213	25	-1.051773563974919
2214	1	-0.9026238094876788
2214	2	-0.9614655350737102
2214	3	-0.9402907374550544
2214	4	-0.8812324715026136
2214	5	-1.0205863601528762
2214	6	-0.8888528317971733
2214	7	-0.8864436849517503
2214	8	-0.9427020666044825
2214	9	-1.0602924699158862
2214	10	-1.1141200522347035
2214	11	-1.1886082256447494
2214	12	-1.2153924574647377
2214	13	-1.0918482589423562
2214	14	-1.3028700646973377
2214	15	-1.3763328136639643
2214	16	-1.3771628913520217
2214	17	-1.2492343257907166
2214	18	-1.2829429195868514
2214	19	-1.2803736299461406
2214	20	-1.2009141463570856
2214	21	-1.3241483369392526
2214	22	-1.2277846732386237
2214	23	-1.429360569671438
2214	24	-1.429292792691598
2214	25	-1.433225628020196
2215	1	-1.5170722730495783
2215	2	-3.225043879136255
2215	3	-1.9074401561957437
2215	4	-1.532209050428726
2215	5	-1.484723730700793
2215	6	-1.405641487560601
2215	7	-1.5216287961200408
2215	8	-1.544091992886436
2215	9	-1.7872812044517872
2215	10	-1.797741038356257
2215	11	-1.8097837841924402
2215	12	-1.8002849197043247
2215	13	-1.878983072299036
2215	14	-2.1518283694069495
2215	15	-2.158780039848402
2215	16	-2.132257580704497
2215	17	-2.2500273381886373
2215	18	-2.1173326035559867
2215	19	-2.116127801515269
2215	20	-2.0550974980512393
2215	21	-2.0886871057403553
2215	22	-2.0585986610996727
2215	23	-2.236574122202107
2215	24	-2.411395618258851
2215	25	-2.447319532145926
2216	1	-1.2075288311659271
2216	2	-1.9125575819076417
2216	3	-1.331117750935018
2216	4	-1.1235633577165913
2216	5	-1.2868214180456548
2216	6	-1.2342869847734121
2216	7	-1.3806105949296494
2216	8	-1.312184365500805
2216	9	-1.5552145302230969
2216	10	-1.4930123578117218
2216	11	-1.4507948985324575
2216	12	-1.6244656400908113
2216	13	-1.8309413951022222
2216	14	-1.9088633819097396
2216	15	-1.9050820989246304
2216	16	-1.7975885254162662
2216	17	-1.8303550765079066
2216	18	-2.0556631778372747
2216	19	-1.8905700531542564
2216	20	-2.0339005045864127
2216	21	-1.9400555908018617
2216	22	-1.9496773754391157
2216	23	-2.043057857167753
2216	24	-1.9810374248110492
2216	25	-2.0622923770866635
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	58813.0
3	2.0
4	1079.0
5	8438.0
6	10425.0
7	8340.0
8	7007.0
9	5788.0
10	5252.0
11	4669.0
12	4451.0
13	4280.0
14	4210.0
15	4331.0
16	4406.0
17	4680.0
18	5334.0
19	6758.0
20	9047.0
21	11826.0
22	14858.0
23	17626.0
24	20894.0
25	24117.0
26	27343.0
27	31215.0
28	35937.0
29	44232.0
30	56978.0
31	74166.0
32	96586.0
33	127599.0
34	173630.0
35	239461.0
36	348286.0
37	608860.0
38	1820300.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.05037083015471	18.84027491889566	13.185724410881699	25.92362984006793
2	15.989187054993787	21.587603069468035	37.53458544780714	24.888624427731042
3	18.137048410367864	25.232497563099944	29.54919383886545	27.081260187666743
4	12.967080990551544	17.62059348437026	37.38822819559506	32.02409732948313
5	14.59587549471947	36.4795595695562	33.903315317158736	15.021249618565594
6	31.279013833027353	37.99237821281021	16.897895474013755	13.83071248014868
7	27.763077295649445	31.806512599056546	22.075313500995144	18.355096604298858
8	25.962667982631306	33.77893829126401	20.407406371066415	19.85098735503827
9	26.796335779521975	15.220598355023574	19.483743704013126	38.49932216144133
10	15.411025295155948	27.584990105921843	32.34987027538051	24.654114323541698
11	34.76078980854128	22.916434536138336	23.459861642573426	18.862914012746955
12	23.957111697129026	24.857779286070077	29.552780026365717	21.632328990435177
13	28.83890976534971	21.335443311716233	25.334369665159663	24.491277257774396
14	22.94892598736384	20.82918797008165	26.613219356724414	29.608666685830094
15	24.077119608718153	27.978081988242614	23.717085963852632	24.2277124391866
16	24.011197746204264	27.362177230994984	24.749598744883663	23.87702627791709
17	23.605626894884683	26.29788312221539	25.96420025754405	24.13228972535588
18	24.431911693368786	25.58974829893384	26.94007012088641	23.038269886810962
19	25.421560075633682	25.54992262887323	26.430704444839588	22.5978128506535
20	24.617208747057724	25.585777267652304	26.47581733064805	23.321196654641916
21	24.781278069197864	25.400081859351996	26.403787406836653	23.414852664613484
22	24.058982168903206	25.415583246125507	26.737233358991176	23.788201225980117
23	23.547903852694404	25.086330951722584	26.770926050337657	24.594839145245356
24	23.522134906575197	25.920613084051286	26.794038069983895	23.763213939389622
25	23.799169348887457	25.484434131330584	26.759079379914137	23.957317139867822
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	921.0
1	921.0
2	1641.5
3	2362.0
4	2362.0
5	2362.0
6	5349.0
7	8336.0
8	8336.0
9	8336.0
10	10820.5
11	13305.0
12	13305.0
13	13305.0
14	17406.5
15	21508.0
16	21508.0
17	21508.0
18	36473.0
19	51438.0
20	51438.0
21	51438.0
22	74715.0
23	97992.0
24	97992.0
25	97992.0
26	132979.5
27	167967.0
28	167967.0
29	167967.0
30	199890.5
31	231814.0
32	231814.0
33	231814.0
34	266420.0
35	301026.0
36	301026.0
37	301026.0
38	331280.0
39	361534.0
40	361534.0
41	361534.0
42	394022.0
43	426510.0
44	426510.0
45	426510.0
46	449132.0
47	471754.0
48	471754.0
49	471754.0
50	473063.5
51	474373.0
52	474373.0
53	474373.0
54	439689.0
55	405005.0
56	405005.0
57	405005.0
58	364155.0
59	323305.0
60	323305.0
61	323305.0
62	278181.5
63	233058.0
64	233058.0
65	233058.0
66	191440.0
67	149822.0
68	149822.0
69	149822.0
70	116248.5
71	82675.0
72	82675.0
73	82675.0
74	65635.0
75	48595.0
76	48595.0
77	48595.0
78	39790.5
79	30986.0
80	30986.0
81	30986.0
82	23511.0
83	16036.0
84	16036.0
85	16036.0
86	11809.5
87	7583.0
88	7583.0
89	7583.0
90	5304.0
91	3025.0
92	3025.0
93	3025.0
94	1638.0
95	251.0
96	251.0
97	251.0
98	147.0
99	43.0
100	43.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.33381460837642424
2	0.009666200654045661
3	0.0
4	0.0
5	0.05028968077117966
6	0.003179671267778178
7	0.0034594823393426575
8	3.5612318199115594E-4
9	0.008826767439352222
10	0.015008048383913
11	0.006257593054987454
12	0.009208327991485602
13	0.017653534878704445
14	0.003179671267778178
15	0.01928152656780687
16	0.011014381271583608
17	0.006257593054987454
18	3.306858118489305E-4
19	0.004349790294320547
20	0.004197166073467195
21	0.002314800682942514
22	5.087474028445085E-5
23	0.0033322954886315305
24	0.0037647307810493628
25	0.002314800682942514
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	3931224.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	61.492511227875056
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.85550792687971	54.02455807624317
2	7.306567760405618	8.985984000879448
3	1.731774531842014	3.194734945303293
4	0.8002531098154886	1.9683829336188345
5	0.467366608585839	1.4369773212999295
6	0.307521320418225	1.1346154949177198
7	0.221156274833968	0.9519618299339961
8	0.16545246027212895	0.8139269816770754
9	0.13429433736166224	0.7432286443246852
>10	0.8676359060919311	10.156370048425845
>50	0.079545087284761	3.381055402451065
>100	0.05297342727466906	6.556146810794123
>500	0.007007337833582659	2.8955634353869346
>1k	0.002736593417436097	2.518463203486095
>5k	1.243906098834587E-4	0.4545344826985225
>10k+	8.292707325563913E-5	0.78349638855909
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	18425	0.46868354487050345	No Hit
TATCAACGCAGAGTACTTTTTTTTT	12304	0.3129814022299416	No Hit
GGTATCAACGCAGAGTACTTTTTTT	6726	0.1710917515766082	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	5728	0.14570525617466723	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	5373	0.1366749897741772	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	4153	0.10564139820066218	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.5437370142225423E-5
2	0.0	0.0	0.0	0.0	2.5437370142225423E-5
3	0.0	0.0	0.0	2.5437370142225423E-5	2.5437370142225423E-5
4	0.0	0.0	0.0	2.5437370142225423E-5	2.5437370142225423E-5
5	0.0	0.0	0.0	2.5437370142225423E-5	2.5437370142225423E-5
6	0.0	0.0	0.0	2.5437370142225423E-5	2.5437370142225423E-5
7	0.0	0.0	0.0	2.5437370142225423E-5	2.5437370142225423E-5
8	0.0	0.0	0.0	2.5437370142225423E-5	5.087474028445085E-5
9	0.0	0.0	0.0	1.017494805689017E-4	5.087474028445085E-5
10	0.0	0.0	0.0	1.271868507111271E-4	5.087474028445085E-5
11	0.0	0.0	0.0	1.271868507111271E-4	5.087474028445085E-5
12	0.0	0.0	0.0	1.5262422085335255E-4	7.631211042667628E-5
13	0.0	0.0	0.0	1.7806159099557797E-4	1.017494805689017E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2165	0.0	15.979414	1
GTATCAA	7235	0.0	13.475635	1
TAGATCG	110	3.8100552E-8	12.093192	5
TTAACCG	65	8.0155017E-4	11.694516	5
CGTCTTA	75	2.0793203E-4	11.397656	15
AACCGCG	270	0.0	10.553117	7
TCGTTAA	290	0.0	10.48087	12
GCGTTAT	175	2.237357E-10	10.347315	1
CGTCGTA	340	0.0	10.336504	10
AGAACCG	425	0.0	10.284295	5
ATACCGT	440	0.0	10.144996	6
CCGTCCG	95	1.6480571E-4	9.998325	9
CGCGTAT	95	1.6491153E-4	9.99769	7
ACCGTCC	155	4.083995E-8	9.804186	8
CGACCAT	375	0.0	9.625054	10
GAACCGC	420	0.0	9.497442	6
GCGGTAA	130	4.267098E-6	9.495751	18
CGCCAGT	420	0.0	9.495751	18
CGGTTCT	525	0.0	9.407829	12
ATAACGC	455	0.0	9.396183	3
>>END_MODULE