FastQCFastQC Report
Thu 2 Feb 2017
SRR4050234_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4050234_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3876429
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNN131680.33969408442667204No Hit
GTATCAACGCAGAGTACTTTTTTTT108750.28054170474939694No Hit
TATCAACGCAGAGTACTTTTTTTTT62120.1602505811405291No Hit
GNNNNNNNNNNNNNNNNNNNNNNNN59290.1529500475824528No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA20500.015.3496211
GTATCAA63200.013.1016091
CGTCGTA3500.012.48475710
GAACCGT658.0315577E-411.6917146
CGAACGA2250.011.39927516
CGGTCCA3300.010.93855510
ACCGTCG4450.010.4597378
ATACCGT4550.010.4390326
TACCGTC4300.010.3831957
CCGTCGT4150.010.3004159
ACGAACG2500.010.25921415
ACCGTCC1701.5643309E-910.0579218
CGACCAT3600.010.02700910
AGAACCG3050.09.9676155
GCTCGTA2500.09.8792449
TAGATCG1451.6182821E-79.8279835
GTCGTAG4500.09.71036711
CGGTTCT5200.09.682075512
CGATAAC2750.09.67198610
GTATAAA5450.09.593781
Produced by FastQC (version 0.11.5)