##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4050234_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3876429
Sequences flagged as poor quality	0
Sequence length	25
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.141681944903414	31.0	31.0	34.0	26.0	34.0
2	30.184729037988312	31.0	31.0	34.0	26.0	34.0
3	30.33923025547482	31.0	31.0	34.0	26.0	34.0
4	33.779487512863	37.0	35.0	37.0	28.0	37.0
5	33.833391505429354	37.0	35.0	37.0	30.0	37.0
6	33.881389288956406	37.0	35.0	37.0	30.0	37.0
7	33.781315999854506	37.0	35.0	37.0	29.0	37.0
8	33.755264961643825	37.0	35.0	37.0	29.0	37.0
9	35.2579299143619	39.0	35.0	39.0	29.0	39.0
10	35.151106340397305	39.0	35.0	39.0	29.0	39.0
11	35.18583417882799	39.0	35.0	39.0	29.0	39.0
12	35.11271533671841	39.0	35.0	39.0	29.0	39.0
13	35.10796070300785	39.0	35.0	39.0	29.0	39.0
14	36.202538470329266	40.0	37.0	41.0	28.0	41.0
15	36.193122071886265	40.0	37.0	41.0	28.0	41.0
16	36.19489896500104	40.0	37.0	41.0	28.0	41.0
17	36.171296314210835	40.0	37.0	41.0	28.0	41.0
18	36.1141963389501	40.0	37.0	41.0	27.0	41.0
19	36.16044844365781	40.0	37.0	41.0	27.0	41.0
20	36.136978389130824	40.0	37.0	41.0	28.0	41.0
21	36.05775908703603	40.0	37.0	41.0	27.0	41.0
22	35.97421544416265	40.0	37.0	41.0	27.0	41.0
23	35.909552838450026	40.0	37.0	41.0	27.0	41.0
24	35.86383008691762	40.0	37.0	41.0	27.0	41.0
25	35.53365765244249	40.0	36.0	41.0	27.0	41.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	1.184341922146885
1101	2	1.1835841316380673
1101	3	1.2655807204362688
1101	4	1.3473084808271096
1101	5	1.2978526806367015
1101	6	1.306065242586378
1101	7	1.2640347702312908
1101	8	1.2631492552467378
1101	9	1.3134188403289855
1101	10	1.3709064907541304
1101	11	1.3732821852958992
1101	12	1.394101189667424
1101	13	1.4142450509780957
1101	14	1.6742376422693752
1101	15	1.6570799699798329
1101	16	1.7357090983663852
1101	17	1.667455633242028
1101	18	1.7044613947932987
1101	19	1.683785374274521
1101	20	1.6741075206281977
1101	21	1.7516299005239162
1101	22	1.7850912082306891
1101	23	1.8973099587039144
1101	24	1.8049779320250394
1101	25	1.7205937141118
1102	1	1.045179767159098
1102	2	1.1050987586114758
1102	3	1.1206732115712228
1102	4	1.215626235376348
1102	5	1.1614331327169154
1102	6	1.140536763270859
1102	7	1.082114157173656
1102	8	1.1228494943437823
1102	9	1.2972727332912726
1102	10	1.3580836074638682
1102	11	1.2804039725582044
1102	12	1.3200781527512078
1102	13	1.325843128814185
1102	14	1.499469405292146
1102	15	1.5203661890274844
1102	16	1.5265416819116027
1102	17	1.5464226255445581
1102	18	1.629709316450402
1102	19	1.597239871465078
1102	20	1.4830650492673527
1102	21	1.584762109867512
1102	22	1.628168509932209
1102	23	1.5921051499958239
1102	24	1.605074455682768
1102	25	1.5315670284205467
1103	1	0.8757119310137327
1103	2	0.962990777155472
1103	3	0.92905319030287
1103	4	1.0073188196015508
1103	5	0.9960123833907986
1103	6	0.9467238390946804
1103	7	0.880501348255045
1103	8	0.9135572423797527
1103	9	1.0365619321290467
1103	10	1.0994864947723784
1103	11	1.038351094854491
1103	12	0.9414604048292006
1103	13	1.0184160695944868
1103	14	1.2131491762190336
1103	15	1.221339919553749
1103	16	1.264217394020342
1103	17	1.2384088878915946
1103	18	1.2866404158298508
1103	19	1.2806277735025517
1103	20	1.2601449719790878
1103	21	1.288970895437565
1103	22	1.316617793661642
1103	23	1.1983896592048424
1103	24	1.1731097879286807
1103	25	1.2966117940742947
1104	1	0.8935089816649118
1104	2	0.9846349418081068
1104	3	0.9234143558020946
1104	4	1.050936634915665
1104	5	1.025265071795964
1104	6	0.9423882494557532
1104	7	0.9212925721398051
1104	8	0.9416277523856422
1104	9	1.1178889510622838
1104	10	1.1126234513572655
1104	11	1.0775704192931101
1104	12	1.138474900950655
1104	13	1.1102018486808518
1104	14	1.2389785007825438
1104	15	1.2477591756254256
1104	16	1.3146994076345138
1104	17	1.343148918799855
1104	18	1.2920175068135933
1104	19	1.3441882276600055
1104	20	1.2634132402452494
1104	21	1.3499434180164087
1104	22	1.408870157492217
1104	23	1.4066257639954287
1104	24	1.3952857960428986
1104	25	1.2943220557532626
1105	1	0.6033205115983904
1105	2	0.7655362897826272
1105	3	0.7431755566608551
1105	4	0.7911391830450398
1105	5	0.7264141936767956
1105	6	0.7127354496809986
1105	7	0.57928363256989
1105	8	0.6798857680826842
1105	9	0.7956332934407726
1105	10	0.79244430800739
1105	11	0.7647998565225436
1105	12	0.7530307380674657
1105	13	0.8398810448234642
1105	14	0.8989932827485418
1105	15	0.8435779909564332
1105	16	0.931380152364035
1105	17	0.9549067409235334
1105	18	0.9666074832328349
1105	19	0.9173633587619676
1105	20	0.9121919682922695
1105	21	0.984116155847687
1105	22	1.0243586662734074
1105	23	1.0069627576251037
1105	24	0.924691349838703
1105	25	0.9472890075007072
1106	1	0.5245469067431401
1106	2	0.6066419934144989
1106	3	0.6281104151651498
1106	4	0.6419865310138135
1106	5	0.6674897886253817
1106	6	0.6020997587068564
1106	7	0.5501290622429522
1106	8	0.5540650798928368
1106	9	0.6284789970122233
1106	10	0.7433734478758183
1106	11	0.7214833800239333
1106	12	0.84296645753912
1106	13	0.73718337225489
1106	14	0.8507632503942943
1106	15	0.9457680200480638
1106	16	0.9562452423024013
1106	17	1.001218603442652
1106	18	0.9989955902022416
1106	19	0.9293752380976414
1106	20	0.9297010739032601
1106	21	0.9617362910044562
1106	22	0.9483109545836825
1106	23	0.9545833408877016
1106	24	0.8754447718063574
1106	25	0.9021267378094748
1107	1	0.6138232664193275
1107	2	0.6981251655533214
1107	3	0.6639908524761502
1107	4	0.7551461356883564
1107	5	0.6710144166861056
1107	6	0.6306483448666214
1107	7	0.6588454848590146
1107	8	0.5829341086310222
1107	9	0.7393152428045369
1107	10	0.7752922681280765
1107	11	0.6812205570341519
1107	12	0.6586699221157417
1107	13	0.7107570122903439
1107	14	0.8129051284785689
1107	15	0.7101659484534792
1107	16	0.8086102455032176
1107	17	0.7797460430355585
1107	18	0.8159122189012109
1107	19	0.6868274804988133
1107	20	0.8597437851622729
1107	21	0.8218030901255275
1107	22	0.8370390414537852
1107	23	0.7805591107669159
1107	24	0.7727088626289387
1107	25	0.7881489051787867
1108	1	0.5093561873620729
1108	2	0.6134437923241123
1108	3	0.5635319315001048
1108	4	0.6839162533741572
1108	5	0.6049820850049272
1108	6	0.49273963138094956
1108	7	0.5319766376470483
1108	8	0.5481143699412385
1108	9	0.6300101135462697
1108	10	0.7146852824646146
1108	11	0.672860119519008
1108	12	0.6183183401750796
1108	13	0.6423641653891892
1108	14	0.7261805347175283
1108	15	0.8134370439249565
1108	16	0.6845246647429377
1108	17	0.7121204156153809
1108	18	0.6729069016712756
1108	19	0.7125556394310735
1108	20	0.7570625294153501
1108	21	0.8002715697196834
1108	22	0.7900365280728252
1108	23	0.6514742885536933
1108	24	0.6439696649867273
1108	25	0.625465766940053
1109	1	0.3541886969042629
1109	2	0.481023239896345
1109	3	0.4187042143088817
1109	4	0.5026340941042378
1109	5	0.48704547477831284
1109	6	0.3475296855521819
1109	7	0.33888177493685845
1109	8	0.34458361088805844
1109	9	0.44005396466183555
1109	10	0.4761539083562525
1109	11	0.41522101269838885
1109	12	0.47028780870336107
1109	13	0.430442375231884
1109	14	0.5033922543786886
1109	15	0.4471441879951712
1109	16	0.49560119019572824
1109	17	0.4743285984331891
1109	18	0.4157519437633539
1109	19	0.4769291035874943
1109	20	0.49656971010178097
1109	21	0.5314800327906468
1109	22	0.49180957646519374
1109	23	0.44035140759547176
1109	24	0.4543801199089259
1109	25	0.5223604339368109
1110	1	0.2663523934098748
1110	2	0.41023169892388367
1110	3	0.40509705182358857
1110	4	0.372316272629142
1110	5	0.3776578996252695
1110	6	0.2872611546534998
1110	7	0.12430236151662655
1110	8	0.27046640188245163
1110	9	0.3594165087869783
1110	10	0.3998378792468671
1110	11	0.3395978613805468
1110	12	0.3199342105203513
1110	13	0.3640684623247452
1110	14	0.4465791827973433
1110	15	0.33548007371737043
1110	16	0.24608170348806624
1110	17	0.3892992131672841
1110	18	0.41382111670318267
1110	19	0.4517028176751552
1110	20	0.43752479951395884
1110	21	0.32642086198251263
1110	22	0.31743643981376124
1110	23	0.28754184681644546
1110	24	0.3965350518686961
1110	25	0.4062899055606479
1111	1	0.0224195169187702
1111	2	0.07068262775339207
1111	3	-0.030792862536571874
1111	4	0.10072286060581348
1111	5	0.11977847994184287
1111	6	0.05399355967956865
1111	7	-0.04621960041426121
1111	8	-0.031533275130250615
1111	9	-0.00790349208075014
1111	10	0.056066351782099844
1111	11	-0.012651648715220176
1111	12	-0.046885931353024546
1111	13	-0.005356114115983246
1111	14	-0.0608193473694314
1111	15	0.06961645630787672
1111	16	-0.061387546434197304
1111	17	-0.05719789320400537
1111	18	-0.08832631494523469
1111	19	-0.12748055474607156
1111	20	-0.09766967981364871
1111	21	0.053835927170510445
1111	22	0.10443263386645896
1111	23	-0.005507804043929809
1111	24	-0.040932390288858755
1111	25	-0.13376779828892893
1112	1	-0.07514738031715495
1112	2	0.021880026270768127
1112	3	-0.09337978530964719
1112	4	-0.020358334679912105
1112	5	-0.04906233540984317
1112	6	-0.0813386070284352
1112	7	-0.12940003234120923
1112	8	-0.09943789662004576
1112	9	-0.1959893538677946
1112	10	-0.220279630709328
1112	11	-0.15082362949805628
1112	12	-0.25148450200734374
1112	13	-0.20543414940869553
1112	14	-0.3693307518209821
1112	15	-0.3346906417345821
1112	16	-0.22043844620546338
1112	17	-0.2684595939049004
1112	18	-0.30937370565448674
1112	19	-0.2746535092476847
1112	20	-0.28170744356742716
1112	21	-0.25190639560808137
1112	22	-0.22908474150212754
1112	23	-0.3224433456979625
1112	24	-0.28861760354245547
1112	25	-0.37373909195699895
1113	1	-0.5635322263069789
1113	2	-0.4502171088655089
1113	3	-0.5335237674068907
1113	4	-0.4520502298812161
1113	5	-0.4868386353655083
1113	6	-0.5132632592124438
1113	7	-0.5298053778349114
1113	8	-0.5708737063610059
1113	9	-0.6662107105084942
1113	10	-0.609608171676669
1113	11	-0.538923466937618
1113	12	-0.4535956764536877
1113	13	-0.5535457038664404
1113	14	-0.684791937878785
1113	15	-0.7069973109346606
1113	16	-0.6909427002219743
1113	17	-0.7635674749191068
1113	18	-0.7500307031421869
1113	19	-0.8302101203904613
1113	20	-0.8258652960807851
1113	21	-0.7938021247021751
1113	22	-0.801843202033723
1113	23	-0.8439467130212037
1113	24	-0.9311509073193491
1113	25	-0.9657815476751139
1114	1	-0.8302543756322756
1114	2	-0.8108507451260571
1114	3	-0.8988584452834836
1114	4	-0.9293655171760946
1114	5	-0.8300300626872783
1114	6	-0.8967529159489303
1114	7	-0.7883160936827025
1114	8	-0.927187794906466
1114	9	-1.0592660635726006
1114	10	-0.9567720763598899
1114	11	-0.9237848007893348
1114	12	-1.2050483501508538
1114	13	-1.0765224092494705
1114	14	-1.1093136530624008
1114	15	-1.132014320216868
1114	16	-1.0872933992224745
1114	17	-1.3589235358933323
1114	18	-1.215966780639718
1114	19	-1.2173288289809605
1114	20	-1.1859720495757884
1114	21	-1.2211352312450785
1114	22	-1.2225042151389687
1114	23	-1.425410645243268
1114	24	-1.2959048397033968
1114	25	-1.306903512328205
1115	1	-1.1082039798918544
1115	2	-1.120139202913414
1115	3	-1.2883493301722488
1115	4	-1.2686743386691646
1115	5	-1.0546603729285948
1115	6	-1.1623615328646153
1115	7	-1.165529963271105
1115	8	-1.1955657875914767
1115	9	-1.844386028941564
1115	10	-1.5037076469630577
1115	11	-1.5337439227575373
1115	12	-1.4828023975944404
1115	13	-1.5830783682900602
1115	14	-1.6657119891485124
1115	15	-1.734103174909663
1115	16	-1.8099921074157592
1115	17	-1.6327765993283023
1115	18	-1.552157095035163
1115	19	-1.5743486487890834
1115	20	-1.6233098206341765
1115	21	-1.5551580687861275
1115	22	-1.8018411993100187
1115	23	-1.7036549161774985
1115	24	-1.918378536895574
1115	25	-1.8170637915267562
1116	1	-1.3786933354816817
1116	2	-1.5479945961820327
1116	3	-1.3324219510558564
1116	4	-1.549699430144564
1116	5	-1.237351299476117
1116	6	-1.298228016580481
1116	7	-1.2292433353113807
1116	8	-1.3285273603635588
1116	9	-1.7887882636629087
1116	10	-2.4438961543264526
1116	11	-1.9197956252583666
1116	12	-1.528681846625254
1116	13	-1.6139447008069183
1116	14	-1.891644887028697
1116	15	-2.6489573434663214
1116	16	-2.1078078487444927
1116	17	-2.1090440250139935
1116	18	-2.014263788615253
1116	19	-1.9350801873901915
1116	20	-1.9791755861547529
1116	21	-1.9491533891768285
1116	22	-1.9112677014721626
1116	23	-1.9653633893699123
1116	24	-1.8794391223381268
1116	25	-2.05558684181554
1201	1	1.0608885711538996
1201	2	1.030716483833114
1201	3	0.9630046002346688
1201	4	1.0077395863609269
1201	5	0.980574143443441
1201	6	0.9650818364416125
1201	7	0.9113249060337552
1201	8	0.9639374323644745
1201	9	1.111584195165733
1201	10	1.1058744854543008
1201	11	1.1287713104521586
1201	12	1.1141048684930581
1201	13	1.067578672625693
1201	14	1.3043302749056167
1201	15	1.3331766071240878
1201	16	1.2181706514976298
1201	17	1.2155171788352206
1201	18	1.3120802926177788
1201	19	1.249971417834459
1201	20	1.3622348356991552
1201	21	1.4118860846636352
1201	22	1.4367091586448169
1201	23	1.43071990470861
1201	24	1.4803253950124926
1201	25	1.5601238713815917
1202	1	0.8866846596018334
1202	2	0.9341811309140375
1202	3	0.772330581307056
1202	4	0.9240781827338509
1202	5	0.8973113020259333
1202	6	0.7877611424158246
1202	7	0.8533135380272725
1202	8	0.9152834074519234
1202	9	0.9172579380892074
1202	10	1.0874580901662227
1202	11	0.9482883429657534
1202	12	1.0884046781037213
1202	13	1.052083792617161
1202	14	1.1940640983489033
1202	15	1.2095316842316919
1202	16	1.1460332616833
1202	17	1.1272702455011583
1202	18	1.2015773717905986
1202	19	1.172601991111108
1202	20	1.1843214973289378
1202	21	1.2688329100579168
1202	22	1.223543669468384
1202	23	1.2520674333706125
1202	24	1.251618388009021
1202	25	1.317785165218659
1203	1	0.7843617291939928
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2211	5	-0.25502640650408637
2211	6	-0.3152377392175367
2211	7	-0.4705252763369998
2211	8	-0.4214187119543027
2211	9	-0.4569094636120923
2211	10	-0.5066216683525226
2211	11	-0.5005081895008985
2211	12	-0.5016950916583625
2211	13	-0.49039713129452167
2211	14	-0.5795801655760684
2211	15	-0.6095627252480469
2211	16	-0.5569370341646618
2211	17	-0.5728957788307767
2211	18	-0.6551483443161459
2211	19	-0.704709906662778
2211	20	-0.6211946640834753
2211	21	-0.6344349405416736
2211	22	-0.6053162768929852
2211	23	-0.6494877169046873
2211	24	-0.5583231991167494
2211	25	-0.5500348195276317
2212	1	-0.6624281942049635
2212	2	-0.49362603480865275
2212	3	-0.5487031635941193
2212	4	-0.5969837257209321
2212	5	-0.641597463113122
2212	6	-0.49083440172161374
2212	7	-0.5265262468587935
2212	8	-0.4795403704009473
2212	9	-0.6651812991300758
2212	10	-0.6462370967617517
2212	11	-0.7408053582457157
2212	12	-0.6655369841572423
2212	13	-0.6753441705627026
2212	14	-0.7722388015318984
2212	15	-0.8218596182976157
2212	16	-0.8765938727457865
2212	17	-0.8579079231600346
2212	18	-0.8247366200030157
2212	19	-0.8502903185151567
2212	20	-0.7947896719503333
2212	21	-0.799495364139041
2212	22	-0.7530292753419658
2212	23	-0.8306762195744355
2212	24	-0.8661443054976274
2212	25	-0.9588813306205282
2213	1	-0.90061494688252
2213	2	-0.7730476010739906
2213	3	-0.7913908869964956
2213	4	-0.8194308025249484
2213	5	-0.7293446939002735
2213	6	-0.7015535441534482
2213	7	-0.7986023703161109
2213	8	-0.8433498346983725
2213	9	-0.841435445900693
2213	10	-0.9255636940915224
2213	11	-0.9383681875545875
2213	12	-0.8981236630869347
2213	13	-0.9440786823180787
2213	14	-1.090085819966923
2213	15	-1.0783523514284568
2213	16	-1.1931607108131885
2213	17	-1.1888686755791582
2213	18	-1.0933262698827306
2213	19	-1.1110867265063007
2213	20	-1.152628312437102
2213	21	-1.206606000783566
2213	22	-1.131698660238463
2213	23	-1.0973432898627635
2213	24	-1.0300508776307993
2213	25	-1.0600899771510086
2214	1	-0.9243509397572716
2214	2	-0.9339890386900684
2214	3	-1.0113015451846685
2214	4	-0.990646721109286
2214	5	-1.014762611973694
2214	6	-1.0718889902065953
2214	7	-1.092378477250115
2214	8	-1.1049380338463806
2214	9	-1.1770294239587855
2214	10	-1.5245204523784253
2214	11	-1.4807363324346952
2214	12	-1.4518412904887725
2214	13	-1.387317633691005
2214	14	-1.6247042185805398
2214	15	-1.6597750887124363
2214	16	-1.5638959165962447
2214	17	-1.5561113345682287
2214	18	-1.5811710540193857
2214	19	-1.5224615823787673
2214	20	-1.5869764071918908
2214	21	-1.5188021247021766
2214	22	-1.4676326757179368
2214	23	-1.6610519761791025
2214	24	-1.6225251763251975
2214	25	-1.6444365184353487
2215	1	-1.9384980665105083
2215	2	-1.627266441729784
2215	3	-1.7205630562713061
2215	4	-2.3264291079103465
2215	5	-1.967886831269393
2215	6	-1.7838924622694918
2215	7	-1.784025004932296
2215	8	-1.8287871801063957
2215	9	-2.0179490362705934
2215	10	-2.0227093458559438
2215	11	-2.130819055225402
2215	12	-2.1873097385633358
2215	13	-2.10189519937213
2215	14	-2.260361820154948
2215	15	-2.261304121990193
2215	16	-2.377163938438116
2215	17	-2.313713418825664
2215	18	-2.5248890929411587
2215	19	-3.0518362031795334
2215	20	-2.664665471468396
2215	21	-2.4630328154948344
2215	22	-3.3722800650427445
2215	23	-2.7052092489017383
2215	24	-2.724070524729626
2215	25	-2.560550000338303
2216	1	-2.939695955972642
2216	2	-5.701774826470583
2216	3	-3.36714314900863
2216	4	-6.020275753787491
2216	5	-3.5635280621618968
2216	6	-2.452054953555951
2216	7	-2.204762885747229
2216	8	-2.0591493090648676
2216	9	-2.3002258709039154
2216	10	-2.3299358328861146
2216	11	-2.2318798742885875
2216	12	-2.343538076068164
2216	13	-2.310091276223467
2216	14	-2.939321659547467
2216	15	-2.804523489967984
2216	16	-2.8921492475851878
2216	17	-2.7343156612721486
2216	18	-2.625912129052921
2216	19	-2.6096252273858624
2216	20	-2.917226540691942
2216	21	-3.183577323649807
2216	22	-3.063425796232657
2216	23	-2.9251052980509655
2216	24	-3.040329523868145
2216	25	-3.2988749065362626
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	134497.0
3	21.0
4	933.0
5	5436.0
6	7621.0
7	7333.0
8	6578.0
9	5846.0
10	5284.0
11	5172.0
12	5092.0
13	4667.0
14	4698.0
15	4842.0
16	4878.0
17	5277.0
18	6090.0
19	7393.0
20	9809.0
21	12584.0
22	15593.0
23	18603.0
24	21923.0
25	24471.0
26	27781.0
27	30883.0
28	35621.0
29	42502.0
30	52760.0
31	68560.0
32	89078.0
33	117770.0
34	161443.0
35	226480.0
36	339042.0
37	598958.0
38	1760910.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.89544220647354	17.989970574826973	12.656114012184508	25.45847320651498
2	15.95892637181652	20.886914757048324	38.66776758927827	24.486391281856886
3	18.31948499567088	24.900476982898674	29.433503553205576	27.346534468224874
4	12.63272596483441	16.918756558659823	38.267074085373935	32.181443391131836
5	14.855555824120472	35.96174353384477	34.0986393894809	15.084061252553857
6	31.459220429122393	37.402050719852	17.21734506346544	13.921383787560165
7	28.03376690964513	31.461928651624206	22.14171464756945	18.36258979116122
8	25.910145612381147	33.53266953779421	20.65025866400296	19.906926185821682
9	26.863349315671936	15.284647884798257	19.550318377644928	38.30168442188488
10	15.511992852468866	27.176470527088842	32.46531612700693	24.84622049343536
11	34.78587450065107	22.894027565880272	23.493818079745083	18.826279853723577
12	24.030830443477434	24.668331528635434	29.576338721088664	21.72449930679847
13	28.78084293896443	21.294610692530743	25.25450980351386	24.670036564990973
14	23.03834156485573	20.848505328827656	26.47603327268001	29.6371198336366
15	24.145910040250417	27.863946568447158	23.578947094808775	24.41119629649365
16	24.154007261521603	27.351956631525216	24.634565185918916	23.85947092103427
17	23.736555604134185	26.340098701841523	25.73988690825553	24.18345878576876
18	24.61652676143986	25.67651908274714	26.507944223681307	23.19900993213169
19	25.592854782909104	25.70847923945726	25.92639286788424	22.772273109749396
20	24.839527775633382	25.682781859926042	25.995135732404464	23.482554632036116
21	24.91012423143254	25.643686212637324	25.885261049761453	23.560928506168686
22	24.24339550248458	25.532633345498894	26.253572984545574	23.970398167470947
23	23.70335214015418	25.292388578878573	26.296894782218367	24.70736449874888
24	23.627064728240992	26.112471560315527	26.308901306074556	23.95156240536892
25	23.95721235676194	25.60209113792964	26.282553159633302	24.158143345675125
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	18495.0
1	18495.0
2	15068.5
3	11642.0
4	11642.0
5	11642.0
6	9055.0
7	6468.0
8	6468.0
9	6468.0
10	8794.5
11	11121.0
12	11121.0
13	11121.0
14	15976.5
15	20832.0
16	20832.0
17	20832.0
18	34810.0
19	48788.0
20	48788.0
21	48788.0
22	70252.5
23	91717.0
24	91717.0
25	91717.0
26	124281.5
27	156846.0
28	156846.0
29	156846.0
30	188544.5
31	220243.0
32	220243.0
33	220243.0
34	256659.5
35	293076.0
36	293076.0
37	293076.0
38	324201.5
39	355327.0
40	355327.0
41	355327.0
42	387233.0
43	419139.0
44	419139.0
45	419139.0
46	441629.5
47	464120.0
48	464120.0
49	464120.0
50	466704.0
51	469288.0
52	469288.0
53	469288.0
54	434706.5
55	400125.0
56	400125.0
57	400125.0
58	360097.0
59	320069.0
60	320069.0
61	320069.0
62	275455.0
63	230841.0
64	230841.0
65	230841.0
66	189756.0
67	148671.0
68	148671.0
69	148671.0
70	115973.0
71	83275.0
72	83275.0
73	83275.0
74	66023.0
75	48771.0
76	48771.0
77	48771.0
78	39819.5
79	30868.0
80	30868.0
81	30868.0
82	23223.5
83	15579.0
84	15579.0
85	15579.0
86	11613.0
87	7647.0
88	7647.0
89	7647.0
90	5394.0
91	3141.0
92	3141.0
93	3141.0
94	1712.5
95	284.0
96	284.0
97	284.0
98	170.0
99	56.0
100	56.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.40730785988857277
2	0.7103444948946569
3	0.7241974507981443
4	0.734258256761571
5	0.743416169882126
6	0.745583112705018
7	0.7632282185485663
8	0.7522387228039001
9	0.7388243148526646
10	0.7322976894456212
11	0.7460216606572698
12	0.7497364197822275
13	0.7489625116311946
14	0.7591780992248278
15	0.7549216043941473
16	0.7487819330626202
17	0.7555149339766057
18	0.758945926779518
19	0.7608033063419968
20	0.7530126309549331
21	0.7525998799410488
22	0.7611386665407777
23	0.7498138105973307
24	0.7546120411337341
25	0.7568047808949938
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	3876429.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	61.749050985982954
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.0632503612275	54.37822136546821
2	7.14839837436998	8.82813631374179
3	1.7247652015898112	3.195078431154553
4	0.7656302670206436	1.891077695786779
5	0.46809092933050606	1.4452085330652782
6	0.3091773780160109	1.145484580729391
7	0.22591599971646	0.9765069009528701
8	0.15879583805307837	0.7844393840241144
9	0.1296954516126763	0.7207713948853111
>10	0.8645560044804518	10.16704841506887
>50	0.08015429470941689	3.4260510092976135
>100	0.05177619900116854	6.474086669646274
>500	0.006767321235923556	2.8197522976702194
>1k	0.002858247434443032	2.8089809343314096
>5k	8.406610101303027E-5	0.3151197738389247
>10k+	8.406610101303027E-5	0.6240363003384619
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
NNNNNNNNNNNNNNNNNNNNNNNNN	13168	0.33969408442667204	No Hit
GTATCAACGCAGAGTACTTTTTTTT	10875	0.28054170474939694	No Hit
TATCAACGCAGAGTACTTTTTTTTT	6212	0.1602505811405291	No Hit
GNNNNNNNNNNNNNNNNNNNNNNNN	5929	0.1529500475824528	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	5.159387673552127E-5	0.0
3	0.0	0.0	0.0	5.159387673552127E-5	0.0
4	0.0	0.0	0.0	7.739081510328191E-5	0.0
5	0.0	0.0	0.0	1.289846918388032E-4	0.0
6	0.0	0.0	0.0	1.289846918388032E-4	0.0
7	0.0	0.0	0.0	1.289846918388032E-4	0.0
8	2.5796938367760636E-5	0.0	0.0	1.289846918388032E-4	0.0
9	2.5796938367760636E-5	0.0	0.0	1.5478163020656382E-4	0.0
10	2.5796938367760636E-5	0.0	0.0	1.5478163020656382E-4	0.0
11	2.5796938367760636E-5	0.0	0.0	1.5478163020656382E-4	0.0
12	2.5796938367760636E-5	0.0	0.0	1.5478163020656382E-4	0.0
13	2.5796938367760636E-5	0.0	0.0	1.8057856857432445E-4	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2050	0.0	15.349621	1
GTATCAA	6320	0.0	13.101609	1
CGTCGTA	350	0.0	12.484757	10
GAACCGT	65	8.0315577E-4	11.691714	6
CGAACGA	225	0.0	11.399275	16
CGGTCCA	330	0.0	10.938555	10
ACCGTCG	445	0.0	10.459737	8
ATACCGT	455	0.0	10.439032	6
TACCGTC	430	0.0	10.383195	7
CCGTCGT	415	0.0	10.300415	9
ACGAACG	250	0.0	10.259214	15
ACCGTCC	170	1.5643309E-9	10.057921	8
CGACCAT	360	0.0	10.027009	10
AGAACCG	305	0.0	9.967615	5
GCTCGTA	250	0.0	9.879244	9
TAGATCG	145	1.6182821E-7	9.827983	5
GTCGTAG	450	0.0	9.710367	11
CGGTTCT	520	0.0	9.6820755	12
CGATAAC	275	0.0	9.671986	10
GTATAAA	545	0.0	9.59378	1
>>END_MODULE