FastQCFastQC Report
Thu 2 Feb 2017
SRR4050234_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4050234_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3876429
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT179850.463957936544175No Hit
TATCAACGCAGAGTACTTTTTTTTT122130.31505800828546066No Hit
GGTATCAACGCAGAGTACTTTTTTT66680.17201398503622795No Hit
ACGCAGAGTACTTTTTTTTTTTTTT57610.14861616193666902No Hit
GTACTTTTTTTTTTTTTTTTTTTTT55000.14188316102268353No Hit
GAGTACTTTTTTTTTTTTTTTTTTT39830.10274920551879062No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA23550.016.653761
GTATCAA75150.012.9772681
TGTGCGA907.5279077E-611.60284910
CGAACGA2550.011.16808516
TCCGCGA600.00590956811.07544610
CGTCGTA3400.010.88930510
CGATAAC2750.010.70146910
CGCGTAT1201.5404476E-610.28381257
TTAGTAC1303.776786E-710.244373
TCCGATA3000.010.1261228
ACGAACG2850.09.99275615
CGCGAAG1054.1413554E-59.9451712
GTATTAG5700.09.8773081
GCGTTAT2053.8198777E-119.7752271
CGAACGT2151.0913936E-119.733984
TAATACC3850.09.6362964
CGACCAT3650.09.62328410
CCGACCA4050.09.610449
AATTCCG3500.09.5127535
TCGAACG2303.6379788E-129.512633
Produced by FastQC (version 0.11.5)