FastQCFastQC Report
Thu 2 Feb 2017
SRR4050227_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4050227_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4285541
Sequences flagged as poor quality0
Sequence length25
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACAAAGGGCAGGGACTTAATCAA87950.2052249645960685No Hit
GTATCTGATCGTCTTCGAACCTCCG65290.15234949333118036No Hit
GAATAGGACCGCGGTTCTATTTTGT63490.14814932350431367No Hit
GATTAAGAGGGACGGCCGGGGGCAT61160.14271243700620295No Hit
GTCCTATTCCATTATTCCTAGCTGC58960.13757889610669924No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG55850.13032193601694628No Hit
CTATTGGAGCTGGAATTACCGCGGC53560.12497838662609924No Hit
GTATCAACGCAGAGTACGGGCGGTG52000.1213382394428148No Hit
GTGTAGCGCGCGTGCAGCCCCGGAC51810.12089488818331222No Hit
ATACAGGACTCTTTCGAGGCCCTGT50520.11788476647405777No Hit
CTCTTAATCATGGCCTCAGTTCCGA50500.11783809792042593No Hit
GAACTACGACGGTATCTGATCGTCT49820.11625136709694295No Hit
CTTTAATATACGCTATTGGAGCTGG48730.11370793092400702No Hit
GTCTTGCGCCGGTCCAAGAATTTCA47080.10985777524937924No Hit
GGGTAGGCACACGCTGAGCCAGTCA46620.10878439851584665No Hit
ATCAGATACCGTCGTAGTTCCGACC46520.10855105574768739No Hit
GACTTAATCAACGCAAGCTTATGAC45940.1071976676923637No Hit
TCGTAGTTCCGACCATAAACGATGC45320.10575094252977628No Hit
GTATCAACGCAGAGTACTTTTTTTT45140.10533092554708963No Hit
ATATTAAAGTTGCTGCAGTTAAAAA44370.10353418623226332No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA30100.016.3526741
CGCTCGA400.005241648814.26611410
CGCTTAC2950.013.214437518
CTCGACG2850.013.01179513
GTATCAA93900.012.9031681
CGGTAGC2450.012.422194510
ACGCTTA3050.012.15770617
CGTCGTA8350.011.73181510
CGTTAGA1052.8548857E-711.7194171
ACGTTCC2050.011.59587816
CGAACGA5100.011.55949916
TCGACGC3400.011.46627614
TAATACC3950.011.2624374
GTGCGAG3300.011.23666818
CGCCAGT7550.011.08211318
CGGTTCT12800.010.99395412
ATCGCCA7650.010.9380216
GACGCTT3400.010.90694516
TCGCCAG7850.010.90083317
GCTCGTA6150.010.8252369
Produced by FastQC (version 0.11.5)