##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR4050227_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4285541 Sequences flagged as poor quality 0 Sequence length 25 %GC 52 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.409087440768854 31.0 31.0 34.0 27.0 34.0 2 30.52528397231528 31.0 31.0 34.0 27.0 34.0 3 30.61758877117265 31.0 31.0 34.0 27.0 34.0 4 34.14138541668368 37.0 35.0 37.0 30.0 37.0 5 34.157822547958354 37.0 35.0 37.0 30.0 37.0 6 34.19134900354471 37.0 35.0 37.0 30.0 37.0 7 34.11825368139052 37.0 35.0 37.0 30.0 37.0 8 34.08848964459796 37.0 35.0 37.0 30.0 37.0 9 35.642343638761126 39.0 37.0 39.0 30.0 39.0 10 35.531317749614345 39.0 37.0 39.0 30.0 39.0 11 35.420684809689135 39.0 37.0 39.0 30.0 39.0 12 35.3438361691091 39.0 37.0 39.0 30.0 39.0 13 35.34095811940663 39.0 37.0 39.0 30.0 39.0 14 36.49983234322108 40.0 37.0 41.0 30.0 41.0 15 36.438636568871935 40.0 37.0 41.0 30.0 41.0 16 36.43657591888632 40.0 37.0 41.0 30.0 41.0 17 36.399392748780144 40.0 37.0 41.0 30.0 41.0 18 36.35659558501482 40.0 37.0 41.0 29.0 41.0 19 36.360568712328266 40.0 37.0 41.0 29.0 41.0 20 36.31038064972427 40.0 37.0 41.0 29.0 41.0 21 36.253769127398385 40.0 37.0 41.0 29.0 41.0 22 36.1626868113034 40.0 37.0 41.0 29.0 41.0 23 36.06922836580026 40.0 37.0 41.0 28.0 41.0 24 35.98965568174473 40.0 37.0 41.0 28.0 41.0 25 35.74252702284262 40.0 36.0 41.0 27.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.665133377289223 1101 2 0.6638263838530456 1101 3 0.6573571517878989 1101 4 0.7287454509485514 1101 5 0.6376165414634372 1101 6 0.6900713611904052 1101 7 0.7142011598625686 1101 8 0.7338594842708162 1101 9 0.7599264267914734 1101 10 0.7853636342085792 1101 11 0.6674248543084147 1101 12 0.7374833429369971 1101 13 0.6431021113904194 1101 14 0.7516002681412459 1101 15 0.7933316827200017 1101 16 0.7794708731184343 1101 17 0.8695824501327607 1101 18 0.793860122295392 1101 19 0.8202473990862202 1101 20 0.7783687216978024 1101 21 0.7336113844438685 1101 22 0.8289299924443583 1101 23 0.8380051565116915 1101 24 0.9221954771879695 1101 25 0.9231653546700755 1102 1 0.5341241324860171 1102 2 0.5280780890765406 1102 3 0.5470895891032868 1102 4 0.453307128225525 1102 5 0.4871547405107677 1102 6 0.4807143016904334 1102 7 0.4975124337790362 1102 8 0.5592826543317386 1102 9 0.6693636545957915 1102 10 0.6945831183876763 1102 11 0.599342767958646 1102 12 0.5080480631475481 1102 13 0.5849242338637168 1102 14 0.707461971486488 1102 15 0.6465824777503428 1102 16 0.6882850203568367 1102 17 0.6625938821897961 1102 18 0.7268975244392948 1102 19 0.7357119760892559 1102 20 0.7681707088279879 1102 21 0.7672249752837601 1102 22 0.7487893323360097 1102 23 0.7119054517367047 1102 24 0.726620726250971 1102 25 0.8007035030793617 1103 1 0.4824382651059409 1103 2 0.48102657461159737 1103 3 0.4487471626646169 1103 4 0.5002172432828331 1103 5 0.48635041101294973 1103 6 0.39300810087281235 1103 7 0.4436091043982131 1103 8 0.44451057966058727 1103 9 0.4941220218867386 1103 10 0.4940211849783367 1103 11 0.465412264551162 1103 12 0.5025176485023977 1103 13 0.4107158811270324 1103 14 0.5562834264291183 1103 15 0.5070053233029341 1103 16 0.5774165069311294 1103 17 0.5046408104232398 1103 18 0.5522845109247285 1103 19 0.5845953629588223 1103 20 0.5619213770225429 1103 21 0.5283247717674371 1103 22 0.6558749706845219 1103 23 0.6476454773198554 1103 24 0.6719687402215371 1103 25 0.6434893319090946 1104 1 0.3590753324357152 1104 2 0.3496065773157184 1104 3 0.3845722610698772 1104 4 0.31692520587633055 1104 5 0.35056996251215367 1104 6 0.28484789927109944 1104 7 0.32019473060619674 1104 8 0.3353457675741396 1104 9 0.3699543571675008 1104 10 0.37345014499936013 1104 11 0.27189850527528847 1104 12 0.2844645450904366 1104 13 0.3131093202867916 1104 14 0.3424314798636843 1104 15 0.1927679566056213 1104 16 0.3193016266889046 1104 17 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-0.6278993085248885 2215 22 -0.7450958529849387 2215 23 -0.5970265184043342 2215 24 -0.6867078169942573 2215 25 -0.6536536979354679 2216 1 -0.5573199063384955 2216 2 -0.7102649067107123 2216 3 -0.6798576009797053 2216 4 -0.555803826652209 2216 5 -0.608167850499548 2216 6 -0.5206153846526078 2216 7 -0.5924005582328533 2216 8 -0.6176080412527369 2216 9 -0.636664975403832 2216 10 -0.6973487033102685 2216 11 -0.4878167335387502 2216 12 -0.5961376753946226 2216 13 -0.597020557370719 2216 14 -0.6144335144279722 2216 15 -0.6143937929735159 2216 16 -0.8200322233690116 2216 17 -0.86496825864171 2216 18 -0.7727093772174385 2216 19 -0.7821622394680006 2216 20 -0.6554977715487738 2216 21 -0.7113467050651678 2216 22 -0.6614132488091613 2216 23 -0.817874963798495 2216 24 -0.9553528420988755 2216 25 -0.9699244806762479 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 126401.0 3 6.0 4 1132.0 5 6052.0 6 7908.0 7 7744.0 8 6877.0 9 6455.0 10 6491.0 11 7493.0 12 12131.0 13 10300.0 14 5101.0 15 5336.0 16 5500.0 17 5977.0 18 6301.0 19 7674.0 20 9788.0 21 11899.0 22 14778.0 23 17766.0 24 20814.0 25 24270.0 26 27178.0 27 31022.0 28 36123.0 29 43456.0 30 55220.0 31 71195.0 32 91639.0 33 121889.0 34 168579.0 35 237610.0 36 356409.0 37 667625.0 38 2043402.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.25826128693201 17.35100427207625 12.687729967018878 25.70300447397286 2 17.635114574466744 20.145455327339924 36.483728009101824 25.735702089091504 3 18.929791340540085 23.787066231677986 27.75680152889623 29.5263408988857 4 13.573312971216843 16.16078559364672 36.0510929518772 34.21480848325923 5 16.609582916533512 34.302685620762695 31.778252131494217 17.309479331209573 6 33.254752446857495 35.79029364157962 15.656455510111062 15.298498401451816 7 30.152005474410377 30.1118952857921 20.32598983797398 19.41010940182354 8 27.768238800171467 30.979623685387757 19.17351620775286 22.07862130668791 9 27.836586361913803 14.915473248703453 18.361525261825 38.88641512755775 10 17.698418054110117 24.861644823037768 28.74191765494017 28.698019467911944 11 37.1049211246311 21.814747982087226 21.278690150254576 19.8016407430271 12 26.186839259964113 23.334436548638287 26.46817090739028 24.010553284007315 13 29.904212939474228 19.577367194693426 23.922195914839243 26.596223950993103 14 24.95860722015979 19.925869988267443 23.564112712380958 31.551410079191804 15 25.826965226413183 25.635129259671015 22.13594914878809 26.40195636512771 16 26.359691004142572 25.74945119996054 22.10473770940436 25.78612008649253 17 26.085478790258954 24.513301171346786 23.248352811934588 26.152867226459676 18 27.772402230621076 23.073101255340998 23.92838878525398 25.22610772878395 19 28.411495690889403 23.692814641180718 23.294566332397103 24.601123335532773 20 27.143794110820757 23.60416942689643 23.234040234048692 26.017996228234118 21 27.46160090496717 23.763914621909375 23.21486957273373 25.55961490038972 22 26.617971969856768 23.530691235753206 23.942973837248907 25.90836295714112 23 25.405867374618303 22.894687027791516 24.309134182480733 27.39031141510945 24 25.71397365809547 24.695529627302566 23.657590473226502 25.932906241375463 25 25.77475470123189 23.828358855710047 23.860887643687406 26.53599879937066 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1816.0 1 1816.0 2 2136.5 3 2457.0 4 2457.0 5 2457.0 6 4537.0 7 6617.0 8 6617.0 9 6617.0 10 9353.0 11 12089.0 12 12089.0 13 12089.0 14 15973.0 15 19857.0 16 19857.0 17 19857.0 18 30574.5 19 41292.0 20 41292.0 21 41292.0 22 58185.5 23 75079.0 24 75079.0 25 75079.0 26 101023.5 27 126968.0 28 126968.0 29 126968.0 30 149891.5 31 172815.0 32 172815.0 33 172815.0 34 215710.5 35 258606.0 36 258606.0 37 258606.0 38 288612.0 39 318618.0 40 318618.0 41 318618.0 42 363126.0 43 407634.0 44 407634.0 45 407634.0 46 436952.5 47 466271.0 48 466271.0 49 466271.0 50 481192.0 51 496113.0 52 496113.0 53 496113.0 54 468403.0 55 440693.0 56 440693.0 57 440693.0 58 424338.0 59 407983.0 60 407983.0 61 407983.0 62 373212.0 63 338441.0 64 338441.0 65 338441.0 66 295444.5 67 252448.0 68 252448.0 69 252448.0 70 207341.0 71 162234.0 72 162234.0 73 162234.0 74 138062.5 75 113891.0 76 113891.0 77 113891.0 78 98873.5 79 83856.0 80 83856.0 81 83856.0 82 65094.5 83 46333.0 84 46333.0 85 46333.0 86 34254.0 87 22175.0 88 22175.0 89 22175.0 90 16191.0 91 10207.0 92 10207.0 93 10207.0 94 5527.5 95 848.0 96 848.0 97 848.0 98 524.0 99 200.0 100 200.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.14287577694391443 2 0.15958311914411738 3 0.1731870025278022 4 0.19407118027805592 5 0.1845274610603422 6 0.0677860741502648 7 0.05460220774926666 8 0.05901238606747666 9 0.06566265496001555 10 0.06776273987344889 11 0.7007283327822555 12 0.6600800225689126 13 0.6575832549496085 14 0.6569998980292103 15 0.6593799942644348 16 0.6656335804511029 17 0.6564865439392599 18 0.6675936597036407 19 0.6626467930186644 20 0.6685036964994618 21 0.6568365580914989 22 0.6595900027557781 23 0.6445627284863218 24 0.6548998131157769 25 0.6480862042855265 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 4285541.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 41.549780478533954 #Duplication Level Percentage of deduplicated Percentage of total 1 83.57943675543179 34.727072497077025 2 10.3719161073203 8.619016748018577 3 2.3530818121705255 2.9331009823114864 4 0.929029168488572 1.544038320354204 5 0.5071176941264769 1.0535314433867722 6 0.32354758980504145 0.8065998798454934 7 0.23813676354435587 0.6926171174395572 8 0.18071189564004952 0.6006831674963036 9 0.14588629246143847 0.5455389083939973 >10 1.0147980246505797 8.469489245058654 >50 0.15309193842927152 4.503599131869072 >100 0.1629408127037038 14.167567236148903 >500 0.02226647883444458 6.386465056966293 >1k 0.01741858723633153 13.425700057594748 >5k 6.200791572804104E-4 1.5249802080388275 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTACAAAGGGCAGGGACTTAATCAA 8795 0.2052249645960685 No Hit GTATCTGATCGTCTTCGAACCTCCG 6529 0.15234949333118036 No Hit GAATAGGACCGCGGTTCTATTTTGT 6349 0.14814932350431367 No Hit GATTAAGAGGGACGGCCGGGGGCAT 6116 0.14271243700620295 No Hit GTCCTATTCCATTATTCCTAGCTGC 5896 0.13757889610669924 No Hit GTTCAAAGCAGGCCCGAGCCGCCTG 5585 0.13032193601694628 No Hit CTATTGGAGCTGGAATTACCGCGGC 5356 0.12497838662609924 No Hit GTATCAACGCAGAGTACGGGCGGTG 5200 0.1213382394428148 No Hit GTGTAGCGCGCGTGCAGCCCCGGAC 5181 0.12089488818331222 No Hit ATACAGGACTCTTTCGAGGCCCTGT 5052 0.11788476647405777 No Hit CTCTTAATCATGGCCTCAGTTCCGA 5050 0.11783809792042593 No Hit GAACTACGACGGTATCTGATCGTCT 4982 0.11625136709694295 No Hit CTTTAATATACGCTATTGGAGCTGG 4873 0.11370793092400702 No Hit GTCTTGCGCCGGTCCAAGAATTTCA 4708 0.10985777524937924 No Hit GGGTAGGCACACGCTGAGCCAGTCA 4662 0.10878439851584665 No Hit ATCAGATACCGTCGTAGTTCCGACC 4652 0.10855105574768739 No Hit GACTTAATCAACGCAAGCTTATGAC 4594 0.1071976676923637 No Hit TCGTAGTTCCGACCATAAACGATGC 4532 0.10575094252977628 No Hit GTATCAACGCAGAGTACTTTTTTTT 4514 0.10533092554708963 No Hit ATATTAAAGTTGCTGCAGTTAAAAA 4437 0.10353418623226332 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 2.3334276815925925E-5 0.0 5 0.0 0.0 0.0 2.3334276815925925E-5 0.0 6 2.3334276815925925E-5 0.0 0.0 2.3334276815925925E-5 0.0 7 2.3334276815925925E-5 0.0 0.0 4.666855363185185E-5 0.0 8 2.3334276815925925E-5 0.0 0.0 4.666855363185185E-5 0.0 9 2.3334276815925925E-5 0.0 0.0 9.33371072637037E-5 0.0 10 2.3334276815925925E-5 0.0 0.0 1.1667138407962961E-4 0.0 11 2.3334276815925925E-5 0.0 0.0 1.6333993771148146E-4 0.0 12 2.3334276815925925E-5 0.0 0.0 1.866742145274074E-4 2.3334276815925925E-5 13 2.3334276815925925E-5 0.0 0.0 1.866742145274074E-4 2.3334276815925925E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3010 0.0 16.352674 1 CGCTCGA 40 0.0052416488 14.266114 10 CGCTTAC 295 0.0 13.2144375 18 CTCGACG 285 0.0 13.011795 13 GTATCAA 9390 0.0 12.903168 1 CGGTAGC 245 0.0 12.4221945 10 ACGCTTA 305 0.0 12.157706 17 CGTCGTA 835 0.0 11.731815 10 CGTTAGA 105 2.8548857E-7 11.719417 1 ACGTTCC 205 0.0 11.595878 16 CGAACGA 510 0.0 11.559499 16 TCGACGC 340 0.0 11.466276 14 TAATACC 395 0.0 11.262437 4 GTGCGAG 330 0.0 11.236668 18 CGCCAGT 755 0.0 11.082113 18 CGGTTCT 1280 0.0 10.993954 12 ATCGCCA 765 0.0 10.93802 16 GACGCTT 340 0.0 10.906945 16 TCGCCAG 785 0.0 10.900833 17 GCTCGTA 615 0.0 10.825236 9 >>END_MODULE