FastQCFastQC Report
Thu 2 Feb 2017
SRR4050227_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4050227_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4285541
Sequences flagged as poor quality0
Sequence length25
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACAAAGGGCAGGGACTTAATCAA85690.19995141803566924No Hit
GTATCAACGCAGAGTACTTTTTTTT68530.15990979901954036No Hit
GATTAAGAGGGACGGCCGGGGGCAT64620.15078609678451332No Hit
GAATAGGACCGCGGTTCTATTTTGT63500.14817265778112962No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG57820.13491878854968367No Hit
GTATCTGATCGTCTTCGAACCTCCG57610.13442876873654924No Hit
ATACAGGACTCTTTCGAGGCCCTGT55020.12838519104122445No Hit
ATCAGATACCGTCGTAGTTCCGACC53550.12495505234928332No Hit
CTATTGGAGCTGGAATTACCGCGGC53220.12418502121435777No Hit
GTCCTATTCCATTATTCCTAGCTGC52770.12313497875764111No Hit
CTCTTAATCATGGCCTCAGTTCCGA51860.12101155956739185No Hit
TCGTAGTTCCGACCATAAACGATGC50430.11767475798271443No Hit
GTATCAACGCAGAGTACGGGCGGTG49850.11632136992739074No Hit
GTGTAGCGCGCGTGCAGCCCCGGAC49390.11524799319385813No Hit
GGGTAGGCACACGCTGAGCCAGTCA48990.1143146221212211No Hit
GAACTACGACGGTATCTGATCGTCT48470.11310123972679295No Hit
CTTTAATATACGCTATTGGAGCTGG48100.11223787148460371No Hit
GAATAACGCCGCCGCATCGCCAGTC45560.1063109651733585No Hit
ATATTAAAGTTGCTGCAGTTAAAAA45300.10570427397614444No Hit
GACTTAATCAACGCAAGCTTATGAC44570.10400087176858183No Hit
CTTATGACCCGCACTTACTGGGAAT44310.10339418057136776No Hit
GTCTTGCGCCGGTCCAAGAATTTCA43900.1024374752219148No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA31700.016.57741
GAACCGT551.9626135E-413.8171896
ACCGCTA2050.012.9687718
GTATCAA95500.012.8295621
ACTCTAA9350.012.08425410
AACCGCG6900.011.1473377
CACTCTA10150.010.9449689
AGAACCG7950.010.8757185
CGTCGTA10000.010.82403910
GCTACGG2200.010.78951311
GAACGCG803.7541514E-410.69347519
CATTCCG1605.511538E-1010.6818689
CGAGCCG11450.010.61089815
CGCTACG2250.010.54974610
GGTAGCG2250.010.54974611
AGGCCCG12100.010.51487110
CGCCAGT8050.010.49401918
CGGTTCT11800.010.379271512
TCGATAA1106.091959E-610.35793210
AACGACG1106.114902E-610.3546715
Produced by FastQC (version 0.11.5)