##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4050227_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4285541
Sequences flagged as poor quality	0
Sequence length	25
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.032191268266946	31.0	31.0	34.0	28.0	34.0
2	31.17836207844004	31.0	31.0	34.0	28.0	34.0
3	31.349921048474393	33.0	31.0	34.0	28.0	34.0
4	34.90751132704133	37.0	35.0	37.0	32.0	37.0
5	34.79120885787815	37.0	35.0	37.0	32.0	37.0
6	34.77848677681534	37.0	35.0	37.0	32.0	37.0
7	34.72356698955861	37.0	35.0	37.0	32.0	37.0
8	34.7041636050151	37.0	35.0	37.0	32.0	37.0
9	36.35224864258678	39.0	37.0	39.0	32.0	39.0
10	36.227780809937414	39.0	37.0	39.0	32.0	39.0
11	36.23157753011814	39.0	37.0	39.0	32.0	39.0
12	36.11290523180154	39.0	37.0	39.0	32.0	39.0
13	36.13758029616331	39.0	37.0	39.0	32.0	39.0
14	37.322435370470146	40.0	37.0	41.0	32.0	41.0
15	37.27699769994033	40.0	37.0	41.0	32.0	41.0
16	37.241556666941236	40.0	37.0	41.0	32.0	41.0
17	37.20769816459579	40.0	37.0	41.0	32.0	41.0
18	37.15851020909612	40.0	37.0	41.0	31.0	41.0
19	37.165354619171765	40.0	37.0	41.0	31.0	41.0
20	37.110701776041815	40.0	37.0	41.0	31.0	41.0
21	37.05877600984333	40.0	37.0	41.0	31.0	41.0
22	37.003598145485014	40.0	37.0	41.0	31.0	41.0
23	36.91731919027259	40.0	37.0	41.0	31.0	41.0
24	36.8319099035571	40.0	37.0	41.0	31.0	41.0
25	36.51293897316582	40.0	37.0	41.0	30.0	41.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.34787856348561164
1101	2	0.33540413277795267
1101	3	0.3852707485861302
1101	4	0.33461052192710383
1101	5	0.4239765715347801
1101	6	0.43091156640404193
1101	7	0.46766193423666635
1101	8	0.49827921782686246
1101	9	0.5439578632346738
1101	10	0.5426082578685225
1101	11	0.5002189863984796
1101	12	0.5543500894946689
1101	13	0.5433311536555365
1101	14	0.7230942017771795
1101	15	0.6272061395878907
1101	16	0.647474906478088
1101	17	0.7052151834022595
1101	18	0.7629890659398839
1101	19	0.6825894065963425
1101	20	0.780329705837417
1101	21	0.6006659667206407
1101	22	0.7151929089842994
1101	23	0.672213322530034
1101	24	0.8282641371498443
1101	25	0.8782567818044456
1102	1	0.43546293978628015
1102	2	0.4109340948743352
1102	3	0.4306920998108872
1102	4	0.3689139528876737
1102	5	0.34418717416119193
1102	6	0.4219160780994926
1102	7	0.4027596333830772
1102	8	0.4463929632936541
1102	9	0.5594142244720715
1102	10	0.5388379706758428
1102	11	0.494748939969071
1102	12	0.5731130638808608
1102	13	0.5151015245560231
1102	14	0.5807902168983219
1102	15	0.6311712282677817
1102	16	0.5354952350725384
1102	17	0.6030746664239146
1102	18	0.6574055231002234
1102	19	0.7511964235319297
1102	20	0.724138829315983
1102	21	0.6738944035007108
1102	22	0.6652788515767654
1102	23	0.782881675064921
1102	24	0.8146070544235471
1102	25	0.9954949307562799
1103	1	0.22337556093269129
1103	2	0.3042163382534788
1103	3	0.30030291669325493
1103	4	0.237197895973857
1103	5	0.3157938436823713
1103	6	0.3719087585262173
1103	7	0.3780327794081444
1103	8	0.35078714754109086
1103	9	0.44410822766491265
1103	10	0.41596958891882707
1103	11	0.41518301182374273
1103	12	0.4473997919271966
1103	13	0.47779704122933
1103	14	0.4799710962115711
1103	15	0.5139446828755183
1103	16	0.5464010064819504
1103	17	0.42162909669415427
1103	18	0.5546657778071804
1103	19	0.5403559188455205
1103	20	0.5332736770403486
1103	21	0.47266971738395824
1103	22	0.6067336938641006
1103	23	0.557193979172574
1103	24	0.6152469906387239
1103	25	0.7133567649351633
1104	1	0.1835551369426014
1104	2	0.22495123304414477
1104	3	0.23093627041919973
1104	4	0.2145245043367936
1104	5	0.25042305966254474
1104	6	0.26327005556661476
1104	7	0.26769216671915785
1104	8	0.2823732254664222
1104	9	0.2996089294528872
1104	10	0.265760993933398
1104	11	0.3034666459025601
1104	12	0.32413437480659724
1104	13	0.3532419110989977
1104	14	0.35799645554697435
1104	15	0.30904032589411656
1104	16	0.311271874303813
1104	17	0.3903820281270711
1104	18	0.43944310588458535
1104	19	0.44137736393198423
1104	20	0.4501316919022287
1104	21	0.5759329467730012
1104	22	0.4674518856235892
1104	23	0.46317607959123563
1104	24	0.5049488416065273
1104	25	0.5627129236505155
1105	1	0.23114885054911483
1105	2	0.2677180385813571
1105	3	0.2492441548244777
1105	4	0.17595313408070012
1105	5	0.18866726371820874
1105	6	0.3381249378468141
1105	7	0.2999462638004786
1105	8	0.33733203254041655
1105	9	0.41100322175337567
1105	10	0.45723234137790314
1105	11	0.49725365770456165
1105	12	0.5135825985042359
1105	13	0.4593087850709736
1105	14	0.5211202984591736
1105	15	0.5471427619316174
1105	16	0.4601665928208192
1105	17	0.5243281447543211
1105	18	0.45662445801146845
1105	19	0.5026453280577599
1105	20	0.5413673596214323
1105	21	0.5475748768734974
1105	22	0.521219999825604
1105	23	0.5414657147258808
1105	24	0.5684880715353273
1105	25	0.6366546940698896
1106	1	0.30381278827241687
1106	2	0.28864958627082515
1106	3	0.2693580606049508
1106	4	0.33433905084004323
1106	5	0.33339636482319435
1106	6	0.3448510576206161
1106	7	0.42128246804532665
1106	8	0.42052106313455795
1106	9	0.4424466592755607
1106	10	0.46209191121440085
1106	11	0.4259690352215344
1106	12	0.47832064360278537
1106	13	0.5054155924438533
1106	14	0.5330062507105495
1106	15	0.5416540365690068
1106	16	0.4736329818608809
1106	17	0.5376315543560963
1106	18	0.5480936864145605
1106	19	0.571487814393322
1106	20	0.542083588947321
1106	21	0.5401522140241966
1106	22	0.5244332123282689
1106	23	0.579150072201692
1106	24	0.6341819882000266
1106	25	0.7731709849153106
1107	1	0.20800261140166043
1107	2	0.21584538290648325
1107	3	0.19978274489914583
1107	4	0.22315761181472737
1107	5	0.18960719056941855
1107	6	0.24666649593933698
1107	7	0.18085344897441047
1107	8	0.22122170173496158
1107	9	0.2520296651565488
1107	10	0.3402298057091073
1107	11	0.24160372021989218
1107	12	0.3452947241425406
1107	13	0.1881008485444866
1107	14	0.25038084134936867
1107	15	0.21355029181628993
1107	16	0.13450597514592744
1107	17	0.1890249278436258
1107	18	0.22776471438277213
1107	19	0.21986766063935193
1107	20	0.1721226851101605
1107	21	0.1435980601310689
1107	22	0.028440229087458135
1107	23	0.30927419577847814
1107	24	0.2904061355463625
1107	25	0.4749655413571858
1108	1	0.14184826641229975
1108	2	0.14173614916854405
1108	3	0.2020709078214047
1108	4	0.17792502982752723
1108	5	0.23301460225950876
1108	6	0.24774123737528697
1108	7	0.2331441899974891
1108	8	0.2141172156403357
1108	9	0.2841218085645565
1108	10	0.23099122659149884
1108	11	0.20718262291440226
1108	12	0.17111177015600987
1108	13	0.16818456802543835
1108	14	0.09662981120930425
1108	15	0.23501359497171137
1108	16	0.17809818547336675
1108	17	0.2426057153081942
1108	18	0.22671515170326018
1108	19	0.17522866725592223
1108	20	0.2748304229381091
1108	21	0.2533384539490555
1108	22	0.25191181487240755
1108	23	0.17895993200600913
1108	24	0.25987470442058935
1108	25	0.5157700904149252
1109	1	0.024392005111582904
1109	2	0.10740817421922344
1109	3	0.026905406759688333
1109	4	0.08045797498926532
1109	5	0.06719844656359442
1109	6	0.09359583462801879
1109	7	0.07184653415193765
1109	8	0.04906394556581262
1109	9	0.13136567756131257
1109	10	0.03986330008576289
1109	11	0.08663235770686839
1109	12	0.1178804010321386
1109	13	0.07572970149308844
1109	14	0.09130871262836138
1109	15	0.0392833909031296
1109	16	0.033329473796861464
1109	17	0.10729641889531649
1109	18	0.021193536274743963
1109	19	0.06580150801477913
1109	20	0.057723160624277625
1109	21	0.10604436413000684
1109	22	0.1345411634922158
1109	23	0.21897723354650367
1109	24	0.21210565131211467
1109	25	0.3438768212968242
1110	1	0.0035768158691631413
1110	2	-0.03483960760938132
1110	3	0.02774129557341709
1110	4	0.09771120454740156
1110	5	0.14182387688920528
1110	6	0.11541178529193985
1110	7	0.15138631176210993
1110	8	0.08927696889902137
1110	9	0.1358668078076093
1110	10	0.14793009087061648
1110	11	0.1334399701930309
1110	12	0.1989096599320206
1110	13	0.11273140082452215
1110	14	0.12682948495612578
1110	15	0.05581459369697939
1110	16	0.12428944838252676
1110	17	0.12368278411975098
1110	18	0.15821789095741678
1110	19	0.15264394454918317
1110	20	0.1589476031189463
1110	21	0.1579547883289436
1110	22	0.2022359562912044
1110	23	0.22552409878786506
1110	24	0.20430922999317858
1110	25	0.3249171917640368
1111	1	-0.11748815274807711
1111	2	-0.1328556269635861
1111	3	-0.08986338338778666
1111	4	-0.09172312955844575
1111	5	-0.11621785587571054
1111	6	-0.055091758845797756
1111	7	0.013835790976791884
1111	8	0.04683838331146006
1111	9	0.08022340825416308
1111	10	0.03379080133008472
1111	11	0.06689824113703224
1111	12	0.08303585433663585
1111	13	0.09646354899904708
1111	14	0.03898974790794085
1111	15	0.10571228049671788
1111	16	0.12340089718195202
1111	17	0.05622859457699292
1111	18	0.08630591104068941
1111	19	0.0900220800434397
1111	20	0.08532455281857665
1111	21	0.01842754578360939
1111	22	0.028944033046769846
1111	23	0.05684699892997003
1111	24	0.004781600660500374
1111	25	0.13221801482631435
1112	1	-0.20819817448489175
1112	2	-0.14319496628605322
1112	3	-0.17734132837564687
1112	4	-0.1497772360898253
1112	5	-0.1626541540135591
1112	6	-0.0507031964622513
1112	7	-0.08122398316722723
1112	8	-0.0908419127602258
1112	9	-0.12029049782530166
1112	10	-0.16670857522412774
1112	11	-0.13302305403905024
1112	12	-0.08182279049518826
1112	13	-0.18121756976665182
1112	14	-0.14343586786463902
1112	15	-0.14501553571111714
1112	16	-0.11417563224915739
1112	17	-0.13889866759743796
1112	18	-0.11495287536683207
1112	19	-0.12014078689406915
1112	20	-0.17912623855300325
1112	21	-0.24804975919480654
1112	22	-0.1223528820638009
1112	23	-0.09559942764307294
1112	24	-0.07847574325575124
1112	25	0.04442326161223775
1113	1	-0.16945120994085627
1113	2	-0.14217195929029103
1113	3	-0.16749635441144406
1113	4	-0.10846075172768366
1113	5	-0.07749686573032477
1113	6	-0.09874009110995985
1113	7	-0.12326794910180894
1113	8	-0.15010839044128943
1113	9	-0.12799490753832998
1113	10	-0.18095968066732837
1113	11	-0.11027436371337274
1113	12	-0.1109459942078388
1113	13	-0.16930404552351064
1113	14	-0.2514395249997321
1113	15	-0.06283124978551058
1113	16	-0.060515263549106635
1113	17	-0.09609938490256553
1113	18	-0.06725913240423154
1113	19	-0.02602210033443697
1113	20	-0.13366607788906748
1113	21	-0.13141234182014472
1113	22	-0.0451498179203611
1113	23	-0.04148935466955095
1113	24	-0.10363530559722278
1113	25	0.010458012056695054
1114	1	-0.28972960809205617
1114	2	-0.26277160360320195
1114	3	-0.21949494411800785
1114	4	-0.20985245769099237
1114	5	-0.21481441091601283
1114	6	-0.19870745386693045
1114	7	-0.20092408242580007
1114	8	-0.1743501346332721
1114	9	-0.24252220197543295
1114	10	-0.18244895660848215
1114	11	-0.24036216150447842
1114	12	-0.2709394933398883
1114	13	-0.23495863489136326
1114	14	-0.28144148627210797
1114	15	-0.42795067570418865
1114	16	-0.4011212530432928
1114	17	-0.2869854869999884
1114	18	-0.27295920126710627
1114	19	-0.3102345792289256
1114	20	-0.24215140033800253
1114	21	-0.14811163623674872
1114	22	-0.18560312055683426
1114	23	-0.35564076071957373
1114	24	-0.4199108854398972
1114	25	-0.29870268677901635
1115	1	-0.41035196525258755
1115	2	-0.3853936776277038
1115	3	-0.32743789861863704
1115	4	-0.3200562881588098
1115	5	-0.4371430380743959
1115	6	-0.33002579637471285
1115	7	-0.4489347588150565
1115	8	-0.40792343765310335
1115	9	-0.5251115523613947
1115	10	-0.4836655807589594
1115	11	-0.5302733069728518
1115	12	-0.4569279961125474
1115	13	-0.4880981757165159
1115	14	-0.6861529356813207
1115	15	-0.6195015377215256
1115	16	-0.6723177664776969
1115	17	-0.6403903442296581
1115	18	-0.5984619737475327
1115	19	-0.6145080102460199
1115	20	-0.5400601312750837
1115	21	-0.5639065974044399
1115	22	-0.49252220683695214
1115	23	-0.5897559780356332
1115	24	-0.7230695370311295
1115	25	-0.5037931648558498
1116	1	-0.19433163997485536
1116	2	-0.21947259687014764
1116	3	-0.2036365586649289
1116	4	-0.18041698855029153
1116	5	-0.30369171064076994
1116	6	-0.24279590942185791
1116	7	-0.3004015622864813
1116	8	-0.3594773320080762
1116	9	-0.3968161762399305
1116	10	-0.38952351146490827
1116	11	-0.36905403460313124
1116	12	-0.32998930785027625
1116	13	-0.3864459529446336
1116	14	-0.4099677728897717
1116	15	-0.4611961455377198
1116	16	-0.44973307273102137
1116	17	-0.39338868913642955
1116	18	-0.36271110497627035
1116	19	-0.39985188487423784
1116	20	-0.4016203772793503
1116	21	-0.4243716114208169
1116	22	-0.5392928206872156
1116	23	-0.5153837836018766
1116	24	-0.5765308805271019
1116	25	-0.44091275297581944
1201	1	0.2711152449253156
1201	2	0.32342364645859334
1201	3	0.3284548880492224
1201	4	0.23822621651490294
1201	5	0.28035117000456466
1201	6	0.384966401112095
1201	7	0.34785010076911504
1201	8	0.31989676378115917
1201	9	0.42903334924410075
1201	10	0.3942864606386749
1201	11	0.4224950817796085
1201	12	0.4677966996395284
1201	13	0.40738750844916893
1201	14	0.501230227059466
1201	15	0.5157468548614546
1201	16	0.5233489621152714
1201	17	0.4705763168974144
1201	18	0.4613601566385839
1201	19	0.4989490768483762
1201	20	0.5986937285239833
1201	21	0.5326418799229842
1201	22	0.5496059746213859
1201	23	0.5412914896514849
1201	24	0.5270882699358808
1201	25	0.5729121249052511
1202	1	0.26929046533439305
1202	2	0.29797318492968117
1202	3	0.28464645676804423
1202	4	0.19669381683569753
1202	5	0.1150436473917722
1202	6	0.29457972459290716
1202	7	0.2758718481541109
1202	8	0.258316192918123
1202	9	0.32418944433982233
1202	10	0.3581744013849999
1202	11	0.4664810266148649
1202	12	0.5463900156061356
1202	13	0.44373052265919455
1202	14	0.4979370114011843
1202	15	0.5237768116154413
1202	16	0.5299841008017907
1202	17	0.4940180550983868
1202	18	0.5057064448749742
1202	19	0.36673170598258764
1202	20	0.47739766895274727
1202	21	0.4097401226628463
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2210	14	-0.07365956275447161
2210	15	-0.07361170433148345
2210	16	-0.016988286848452105
2210	17	-0.0060008084421951935
2210	18	0.016501026740243674
2210	19	0.05406862644842647
2210	20	-0.032430071354504264
2210	21	-0.004364293361263094
2210	22	-0.02199570960891606
2210	23	-0.06716650142648461
2210	24	-0.17005289996917128
2210	25	-0.3331743329491559
2211	1	-0.16244208912345925
2211	2	-0.1836746503623523
2211	3	-0.1999525260456707
2211	4	-0.17076092736805748
2211	5	-0.15606294484873473
2211	6	-0.21843752010340012
2211	7	-0.18940650587882146
2211	8	-0.30142688201441104
2211	9	-0.35311608750878776
2211	10	-0.25407288400895567
2211	11	-0.3162552007838926
2211	12	-0.2992718531665375
2211	13	-0.266410883016043
2211	14	-0.3188442675870249
2211	15	-0.30224275051511995
2211	16	-0.2585618216218819
2211	17	-0.3621472981701004
2211	18	-0.381432953964314
2211	19	-0.3376619157188685
2211	20	-0.3513081281560986
2211	21	-0.3254845534920605
2211	22	-0.38262615402054934
2211	23	-0.3958134958594499
2211	24	-0.353300221803984
2211	25	-0.48139105609831034
2212	1	-0.19165630662892497
2212	2	-0.1225427176116014
2212	3	-0.12967892209859855
2212	4	-0.1272796632537947
2212	5	-0.06096825706985953
2212	6	-0.10932602550245463
2212	7	-0.14442690728456142
2212	8	-0.19622468193154674
2212	9	-0.16675804146551343
2212	10	-0.17448140626805042
2212	11	-0.23949297419461857
2212	12	-0.18996020897077415
2212	13	-0.15354475686731206
2212	14	-0.16178336661398873
2212	15	-0.11272541132638025
2212	16	-0.2017876299808563
2212	17	-0.19948729780688268
2212	18	-0.14345029209718518
2212	19	-0.10018097934482029
2212	20	-0.16798074989616651
2212	21	-0.15289216644100634
2212	22	-0.2041457831672986
2212	23	-0.3552061670646438
2212	24	-0.25360638015234116
2212	25	-0.2965297185043525
2213	1	-0.31097281225717666
2213	2	-0.2717040132178745
2213	3	-0.2917260667130961
2213	4	-0.33615414374448704
2213	5	-0.24969756954678957
2213	6	-0.3773083736910934
2213	7	-0.4150625245585502
2213	8	-0.3038054892088269
2213	9	-0.5215463554523012
2213	10	-0.4810155280268589
2213	11	-0.45680507912121726
2213	12	-0.42327770759219874
2213	13	-0.4204758613444213
2213	14	-0.43273609554302084
2213	15	-0.4966745776226418
2213	16	-0.48837961532918683
2213	17	-0.5298458049093284
2213	18	-0.5362282233800428
2213	19	-0.4146067859138469
2213	20	-0.33604921746233885
2213	21	-0.45722341200086447
2213	22	-0.4855868225121185
2213	23	-0.4483449532115671
2213	24	-0.4623753376386972
2213	25	-0.4710851793688775
2214	1	-0.34699831897403044
2214	2	-0.37826092785002885
2214	3	-0.3361962737017521
2214	4	-0.25421973828873945
2214	5	-0.3226242497605156
2214	6	-0.34964912584300123
2214	7	-0.3585186347954661
2214	8	-0.3973022473762313
2214	9	-0.42438735023313967
2214	10	-0.4004541842649445
2214	11	-0.38052474797712676
2214	12	-0.4933348709218137
2214	13	-0.45677827295680373
2214	14	-0.5004627175887464
2214	15	-0.5081627593801414
2214	16	-0.5011358956423564
2214	17	-0.541561669020048
2214	18	-0.5496270938687928
2214	19	-0.5283978409417287
2214	20	-0.5003313628729202
2214	21	-0.5864094352262583
2214	22	-0.5829027105373541
2214	23	-0.6640331888666253
2214	24	-0.7281253711841984
2214	25	-1.5867507366927995
2215	1	-0.3928536468135846
2215	2	-0.5058187436959791
2215	3	-0.49688811928260535
2215	4	-0.4002364071365463
2215	5	-0.43500433308689423
2215	6	-0.4955115667065684
2215	7	-0.4444220541396291
2215	8	-0.5007438356160563
2215	9	-0.4766528741797984
2215	10	-0.498714180613959
2215	11	-0.5781892151237074
2215	12	-0.5734945829205529
2215	13	-0.6281489427595517
2215	14	-0.6684633739557384
2215	15	-0.6370336466024682
2215	16	-0.6741206900055019
2215	17	-0.7342637302053134
2215	18	-0.6693573284640237
2215	19	-0.6372549927241735
2215	20	-0.617576643478742
2215	21	-0.624643943768497
2215	22	-0.6836197168529026
2215	23	-0.6794057356820886
2215	24	-0.6940621233020181
2215	25	-0.6500592502561489
2216	1	-0.371061474915642
2216	2	-0.2648976731342856
2216	3	-0.34358068298831057
2216	4	-0.2571437421118503
2216	5	-0.2963871984744557
2216	6	-0.3436650637886842
2216	7	-0.3410147567523225
2216	8	-0.4088219226468439
2216	9	-0.3985571555022531
2216	10	-0.38179726119528823
2216	11	-0.47768621036500747
2216	12	-0.4616578747007978
2216	13	-0.4907630679519812
2216	14	-0.5176631066544957
2216	15	-0.5205162905679686
2216	16	-0.4700994487209087
2216	17	-0.5572581824549729
2216	18	-0.5498410056531498
2216	19	-0.44245143910137585
2216	20	-0.4831122783876154
2216	21	-0.35876781516161316
2216	22	-0.48833132765626885
2216	23	-0.5690835956377427
2216	24	-0.5731331463679581
2216	25	-0.7253543557038711
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	46777.0
3	2.0
4	826.0
5	7121.0
6	8483.0
7	6853.0
8	5697.0
9	4971.0
10	4581.0
11	4358.0
12	4108.0
13	4110.0
14	4052.0
15	4092.0
16	4408.0
17	4833.0
18	5209.0
19	6106.0
20	7425.0
21	9512.0
22	12415.0
23	15417.0
24	18640.0
25	22182.0
26	25858.0
27	30115.0
28	35819.0
29	45229.0
30	59123.0
31	77828.0
32	102768.0
33	138614.0
34	194339.0
35	274152.0
36	409619.0
37	730587.0
38	1949312.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.988407282182834	17.916625332183358	13.321063313535575	25.77390407209823
2	18.01514255592119	20.547388042113162	35.658208347120436	25.77926105484521
3	19.091290108639697	23.817095875220005	28.16191361190934	28.929700404230964
4	14.000115460243462	16.563847060520096	35.70729566166316	33.72874181757328
5	16.480853217021767	34.58333411131629	31.85833210412026	17.07748056754168
6	33.14813556289963	36.006767075152695	15.577934981803676	15.267162380143997
7	30.05600743341676	30.144746180568525	20.324301482947966	19.474944903066753
8	27.84140054100354	31.08795858685581	19.100202351194852	21.970438520945798
9	27.713164496623726	14.85182882832621	18.372436280058444	39.062570394991624
10	17.732841412472933	24.969581710888427	28.826688457201648	28.47088841943699
11	37.170072505380716	21.769121951902743	21.297370104252746	19.7634354384638
12	26.184573520387456	23.404507608108812	26.498659369741556	23.912259501762183
13	30.049894719598026	19.45642035431995	23.972498047359494	26.52118687872253
14	24.939485844949818	19.81118976907663	23.668577746425566	31.580746639547986
15	25.867433243267275	25.72858885329235	22.08545207149996	26.318525831940416
16	26.46113192365977	25.677297167880646	22.11678787830703	25.74478303015255
17	26.15900136158873	24.526191623487463	23.276003486227186	26.03880352869662
18	27.695889806164846	23.0462123202674	24.122426082642576	25.135471790925173
19	28.43405301687698	23.6310888170245	23.424113781667238	24.510744384431277
20	27.211354700206513	23.442065594044966	23.381488525125715	25.965091180622807
21	27.535980955452192	23.648201354744604	23.33815809522787	25.47765959457533
22	26.64824036177471	23.444163395978105	24.03897758508846	25.868618657158727
23	25.40841439474213	22.749167723904844	24.530578081579975	27.311839799773054
24	25.791669583479177	24.448302415120757	23.884400886711003	25.87562711468907
25	25.765900623890186	23.700139200488664	24.09963081609529	26.434329359525865
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	378.0
1	378.0
2	668.5
3	959.0
4	959.0
5	959.0
6	2554.5
7	4150.0
8	4150.0
9	4150.0
10	6043.0
11	7936.0
12	7936.0
13	7936.0
14	11382.0
15	14828.0
16	14828.0
17	14828.0
18	26135.5
19	37443.0
20	37443.0
21	37443.0
22	56019.5
23	74596.0
24	74596.0
25	74596.0
26	101813.5
27	129031.0
28	129031.0
29	129031.0
30	152420.0
31	175809.0
32	175809.0
33	175809.0
34	218085.5
35	260362.0
36	260362.0
37	260362.0
38	290035.5
39	319709.0
40	319709.0
41	319709.0
42	364955.0
43	410201.0
44	410201.0
45	410201.0
46	440081.0
47	469961.0
48	469961.0
49	469961.0
50	484269.5
51	498578.0
52	498578.0
53	498578.0
54	471413.0
55	444248.0
56	444248.0
57	444248.0
58	426574.5
59	408901.0
60	408901.0
61	408901.0
62	373378.0
63	337855.0
64	337855.0
65	337855.0
66	295345.5
67	252836.0
68	252836.0
69	252836.0
70	207278.5
71	161721.0
72	161721.0
73	161721.0
74	137096.0
75	112471.0
76	112471.0
77	112471.0
78	97720.0
79	82969.0
80	82969.0
81	82969.0
82	64983.5
83	46998.0
84	46998.0
85	46998.0
86	34676.0
87	22354.0
88	22354.0
89	22354.0
90	16304.0
91	10254.0
92	10254.0
93	10254.0
94	5556.5
95	859.0
96	859.0
97	859.0
98	496.5
99	134.0
100	134.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.032341307666873334
2	0.0031501273701499996
3	0.008563679591444814
4	0.1636199490332726
5	0.022330902912841108
6	0.08475009339544295
7	0.050892057735534436
8	0.021257526179308518
9	0.0391549164971237
10	0.02594771581930963
11	0.04015829040020852
12	0.03178128502329111
13	0.007420300027464443
14	0.004200169826866666
15	0.003920158505075555
16	0.004643521086369258
17	0.002473433342488148
18	0.0034301386919411113
19	0.0
20	0.002123419190249259
21	2.3334276815925923E-4
22	2.3334276815925925E-5
23	9.33371072637037E-5
24	9.567053494529628E-4
25	0.14238575713077997
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	4285541.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	40.57091694393454
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.90390600284552	33.63487484769202
2	10.887395750485954	8.83423257457423
3	2.4157114714819397	2.94022888410011
4	0.9425186181027945	1.5295537829264174
5	0.514519497768664	1.043726390500369
6	0.33822106529053375	0.8233163249154786
7	0.2554811696608191	0.725557372053384
8	0.17937522678655266	0.5821933942205321
9	0.15133707366295662	0.5525895461506125
>10	1.0392168855824648	8.461261426723766
>50	0.1619535747308114	4.647929663201758
>100	0.168427061869638	14.322654798047738
>500	0.023221093522344572	6.53703300070918
>1k	0.01802234123839066	13.690111036492913
>5k	6.93166970707333E-4	1.6747369576915023
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACAAAGGGCAGGGACTTAATCAA	8569	0.19995141803566924	No Hit
GTATCAACGCAGAGTACTTTTTTTT	6853	0.15990979901954036	No Hit
GATTAAGAGGGACGGCCGGGGGCAT	6462	0.15078609678451332	No Hit
GAATAGGACCGCGGTTCTATTTTGT	6350	0.14817265778112962	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG	5782	0.13491878854968367	No Hit
GTATCTGATCGTCTTCGAACCTCCG	5761	0.13442876873654924	No Hit
ATACAGGACTCTTTCGAGGCCCTGT	5502	0.12838519104122445	No Hit
ATCAGATACCGTCGTAGTTCCGACC	5355	0.12495505234928332	No Hit
CTATTGGAGCTGGAATTACCGCGGC	5322	0.12418502121435777	No Hit
GTCCTATTCCATTATTCCTAGCTGC	5277	0.12313497875764111	No Hit
CTCTTAATCATGGCCTCAGTTCCGA	5186	0.12101155956739185	No Hit
TCGTAGTTCCGACCATAAACGATGC	5043	0.11767475798271443	No Hit
GTATCAACGCAGAGTACGGGCGGTG	4985	0.11632136992739074	No Hit
GTGTAGCGCGCGTGCAGCCCCGGAC	4939	0.11524799319385813	No Hit
GGGTAGGCACACGCTGAGCCAGTCA	4899	0.1143146221212211	No Hit
GAACTACGACGGTATCTGATCGTCT	4847	0.11310123972679295	No Hit
CTTTAATATACGCTATTGGAGCTGG	4810	0.11223787148460371	No Hit
GAATAACGCCGCCGCATCGCCAGTC	4556	0.1063109651733585	No Hit
ATATTAAAGTTGCTGCAGTTAAAAA	4530	0.10570427397614444	No Hit
GACTTAATCAACGCAAGCTTATGAC	4457	0.10400087176858183	No Hit
CTTATGACCCGCACTTACTGGGAAT	4431	0.10339418057136776	No Hit
GTCTTGCGCCGGTCCAAGAATTTCA	4390	0.1024374752219148	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	2.3334276815925925E-5	0.0
6	0.0	0.0	0.0	2.3334276815925925E-5	2.3334276815925925E-5
7	0.0	0.0	0.0	2.3334276815925925E-5	2.3334276815925925E-5
8	0.0	0.0	0.0	4.666855363185185E-5	4.666855363185185E-5
9	0.0	0.0	0.0	7.000283044777778E-5	4.666855363185185E-5
10	0.0	0.0	0.0	9.33371072637037E-5	4.666855363185185E-5
11	0.0	0.0	0.0	1.1667138407962961E-4	4.666855363185185E-5
12	0.0	0.0	0.0	1.1667138407962961E-4	7.000283044777778E-5
13	0.0	0.0	0.0	1.4000566089555555E-4	7.000283044777778E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	3170	0.0	16.5774	1
GAACCGT	55	1.9626135E-4	13.817189	6
ACCGCTA	205	0.0	12.968771	8
GTATCAA	9550	0.0	12.829562	1
ACTCTAA	935	0.0	12.084254	10
AACCGCG	690	0.0	11.147337	7
CACTCTA	1015	0.0	10.944968	9
AGAACCG	795	0.0	10.875718	5
CGTCGTA	1000	0.0	10.824039	10
GCTACGG	220	0.0	10.789513	11
GAACGCG	80	3.7541514E-4	10.693475	19
CATTCCG	160	5.511538E-10	10.681868	9
CGAGCCG	1145	0.0	10.610898	15
CGCTACG	225	0.0	10.549746	10
GGTAGCG	225	0.0	10.549746	11
AGGCCCG	1210	0.0	10.514871	10
CGCCAGT	805	0.0	10.494019	18
CGGTTCT	1180	0.0	10.3792715	12
TCGATAA	110	6.091959E-6	10.357932	10
AACGACG	110	6.114902E-6	10.35467	15
>>END_MODULE