FastQCFastQC Report
Thu 2 Feb 2017
SRR4050226_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4050226_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences528694
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACGGAAGCAGTGGTATCAACGCA491959.305004407086141No Hit
CTTCCGTACTCTGCGTTGATACCAC293805.557089734326472No Hit
GAGTACGGAAGCAGTGGTATCAACG264445.0017590515496675No Hit
TCCGTACTCTGCGTTGATACCACTG251024.747926021479343No Hit
GCTTCCGTACTCTGCGTTGATACCA187083.538530794750839No Hit
GTACATGGGAAGCAGTGGTATCAAC127142.4047936991908365No Hit
GCGTTGATACCACTGCTTCCGTACT122112.309653599246445No Hit
CCCATGTACTCTGCGTTGATACCAC118322.237967519964289No Hit
GTATCAACGCAGAGTACGGAAGCAG110842.0964868146791904No Hit
GTACATGGAAGCAGTGGTATCAACG99531.8825634488002512No Hit
TCCATGTACTCTGCGTTGATACCAC80261.5180804018959928No Hit
TATCAACGCAGAGTACGGAAGCAGT75431.4267232085100268No Hit
ACTCTGCGTTGATACCACTGCTTCC74141.4023234612081847No Hit
CAGTGGTATCAACGCAGAGTACGGA55821.0558092204564455No Hit
GGTATCAACGCAGAGTACGGAAGCA53481.0115492137228719No Hit
CATGTACTCTGCGTTGATACCACTG52330.9897975010119275No Hit
ATACCACTGCTTCCGTACTCTGCGT51090.9663434803496919No Hit
ACGCAGAGTACGGAAGCAGTGGTAT49740.9408088610803225No Hit
GAGTACATGGGAAGCAGTGGTATCA45990.8698793631098519No Hit
GTACGGGAAGCAGTGGTATCAACGC39310.7435302840584535No Hit
AAGCAGTGGTATCAACGCAGAGTAC38120.7210219900358241No Hit
GAAGCAGTGGTATCAACGCAGAGTA36130.6833820697794943No Hit
GAGTACATGGAAGCAGTGGTATCAA35920.679410017893148No Hit
GTGGTATCAACGCAGAGTACGGAAG35430.6701418968250065No Hit
GTATCAACGCAGAGTACATGGGAAG34440.6514165093608023No Hit
GTACTCTGCGTTGATACCACTGCTT33700.6374197550946293No Hit
GCGTTGATACCACTGCTTCCCATGT30190.5710297449942688No Hit
GTTGATACCACTGCTTCCGTACTCT29190.5521152122021434No Hit
CCCGTACTCTGCGTTGATACCACTG26990.5105032400594672No Hit
GATACCACTGCTTCCGTACTCTGCG26880.5084226414523335No Hit
CCACTGCTTCCGTACTCTGCGTTGA25810.48818409136475915No Hit
TTCCCGTACTCTGCGTTGATACCAC25510.48250973152712157No Hit
ATCAACGCAGAGTACGGAAGCAGTG24820.469458703900555No Hit
CGGAAGCAGTGGTATCAACGCAGAG23200.43881716077731164No Hit
GAGTACGGGAAGCAGTGGTATCAAC22840.43200792897214646No Hit
TATCAACGCAGAGTACATGGGAAGC22800.43125134766046147No Hit
CTGCTTCCGTACTCTGCGTTGATAC22480.4251986971669812No Hit
GCTTCCCATGTACTCTGCGTTGATA22030.41668715741052476No Hit
CAGTGGTATCAACGCAGAGTACATG20510.38793706756649404No Hit
GCTTCCATGTACTCTGCGTTGATAC20010.3784798011704313No Hit
CATGGAAGCAGTGGTATCAACGCAG19960.37753407453082505No Hit
CTTCCCGTACTCTGCGTTGATACCA19340.3658070641997072No Hit
GCGTTGATACCACTGCTTCCATGTA18780.3552149258361169No Hit
GTATCAACGCAGAGTACGGGAAGCA18760.3548366351802744No Hit
ACGCAGAGTACATGGGAAGCAGTGG18730.3542691991965107No Hit
AAAAAGTACTCTGCGTTGATACCAC18130.34292047952123533No Hit
GTATCAACGCAGAGTACATGGAAGC17030.3221144934498973No Hit
GTATCAACGCAGAGTACTTTTTTTT15650.2960124381967641No Hit
GCAGAGTACGGAAGCAGTGGTATCA15420.29166209565457524No Hit
GCAGTGGTATCAACGCAGAGTACGG15410.291472950326654No Hit
GCGTTGATACCACTGCTTCCCGTAC14890.28163739327474874No Hit
TGATACCACTGCTTCCGTACTCTGC14880.2814482479468275No Hit
CACTGCTTCCGTACTCTGCGTTGAT14740.2788002133559299No Hit
GGTATCAACGCAGAGTACATGGGAA14270.2699103829436309No Hit
GTACTTTTTTTTTTTTTTTTTTTTT13690.2589399539241981No Hit
AAAGTACTCTGCGTTGATACCACTG13650.2581833726125131No Hit
TACCACTGCTTCCGTACTCTGCGTT13410.25364388474240296No Hit
CTGCGTTGATACCACTGCTTCCGTA13230.2502392688398204No Hit
GCCCATGTACTCTGCGTTGATACCA13110.24796952490476531No Hit
CCATGTACTCTGCGTTGATACCACT13020.24626721695347403No Hit
TATCAACGCAGAGTACATGGAAGCA11470.21694969112567952No Hit
GCTTCCCGTACTCTGCGTTGATACC11420.21600396448607323No Hit
CCCCATGTACTCTGCGTTGATACCA11390.21543652850230946No Hit
ATACCACTGCTTCCCATGTACTCTG11380.21524738317438818No Hit
GGGAAGCAGTGGTATCAACGCAGAG11280.21335592989517568No Hit
ACGCAGAGTACATGGAAGCAGTGGT10990.20787071538545926No Hit
TATCAACGCAGAGTACGGGAAGCAG10900.20616840743416798No Hit
TATCAACGCAGAGTACTTTTTTTTT10020.1895236185770975No Hit
CTGCTTCCCATGTACTCTGCGTTGA9850.1863081480024362No Hit
TACCACTGCTTCCCATGTACTCTGC9600.18157951480440482No Hit
TACGGAAGCAGTGGTATCAACGCAG9240.17477028299923963No Hit
GTGGTATCAACGCAGAGTACATGGG9060.17136566709665704No Hit
GCAGTGGTATCAACGCAGAGTACAT8990.17004164980120828No Hit
CTCTGCGTTGATACCACTGCTTCCG8950.16928506848952324No Hit
TCAACGCAGAGTACGGAAGCAGTGG8950.16928506848952324No Hit
CATGGGAAGCAGTGGTATCAACGCA8790.16625874324278317No Hit
GGTATCAACGCAGAGTACGGGAAGC8410.15907122078177546No Hit
ACGGAAGCAGTGGTATCAACGCAGA7970.15074882635324025No Hit
GCAGAGTACATGGGAAGCAGTGGTA7820.14791164643442142No Hit
ATACCACTGCTTCCATGTACTCTGC7780.1471550651227364No Hit
AGCAGTGGTATCAACGCAGAGTACG7580.1433721585643113No Hit
GGTATCAACGCAGAGTACATGGAAG7530.14242643192470503No Hit
TCCCATGTACTCTGCGTTGATACCA7510.1420481412688625No Hit
CTTCCCATGTACTCTGCGTTGATAC7000.1324017295448785No Hit
GTTGATACCACTGCTTCCCATGTAC6830.12918625897021718No Hit
GAGTACTTTTTTTTTTTTTTTTTTT6830.12918625897021718No Hit
GGTATCAACGCAGAGTACTTTTTTT6640.12559249773971334No Hit
CCACTGCTTCCCATGTACTCTGCGT6400.12105300986960321No Hit
TTGATACCACTGCTTCCGTACTCTG5710.10800198224303661No Hit
GATACCACTGCTTCCCATGTACTCT5700.10781283691511537No Hit
ACATGGGAAGCAGTGGTATCAACGC5480.10365163970084774No Hit
ATCAACGCAGAGTACATGGGAAGCA5480.10365163970084774No Hit
CAACGCAGAGTACGGAAGCAGTGGT5410.10232762240539896No Hit
GTGGTATCAACGCAGAGTACATGGA5330.10081445978202892No Hit
CCGTACTCTGCGTTGATACCACTGC5330.10081445978202892No Hit
AACGCAGAGTACGGAAGCAGTGGTA5300.10024702379826517No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATGGTA504.725871E-617.0323544
ACATGGT508.995594E-515.1398713
ACCCCGT456.8882236E-414.7388521
CCCCGTA1001.0586518E-813.2473872
AAAAAGT3700.013.0601021
CCCCATG1700.012.8190861
GCCCATG2500.012.50696851
AAAAGTA4100.012.2319092
ATGGTAA550.003032945612.1072615
ACATGGA22700.010.2544283
TGGGCAG1054.029882E-59.965846
AGCAGAG1451.5748992E-79.8408778
CATGGAA23900.09.699974
ATGGAAG23850.09.5726765
TGGAAGC24000.09.55248456
ATGGAAA800.0044570899.5128485
GCTCCCG900.001138079.4749771
TCCATGT16800.09.305781
CCATGTA46000.09.1744332
TACATGG67600.09.0564882
Produced by FastQC (version 0.11.5)