##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4050226_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	528694
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.50751852678487	31.0	31.0	34.0	27.0	34.0
2	30.552642927667044	31.0	31.0	34.0	27.0	34.0
3	30.714702644629973	31.0	31.0	34.0	27.0	34.0
4	34.11188702727854	37.0	35.0	37.0	30.0	37.0
5	34.14209164469428	37.0	35.0	37.0	30.0	37.0
6	34.15169266153957	37.0	35.0	37.0	30.0	37.0
7	34.00190469345217	37.0	35.0	37.0	30.0	37.0
8	33.90646763534294	37.0	35.0	37.0	30.0	37.0
9	35.28572482381113	39.0	37.0	39.0	29.0	39.0
10	35.32909017314363	39.0	37.0	39.0	29.0	39.0
11	35.145350618694366	39.0	37.0	39.0	28.0	39.0
12	35.12294257169554	39.0	37.0	39.0	28.0	39.0
13	35.029264565135975	39.0	37.0	39.0	27.0	39.0
14	36.20628756899076	40.0	37.0	41.0	27.0	41.0
15	36.129608053051484	40.0	37.0	41.0	27.0	41.0
16	35.918843414148824	40.0	36.0	41.0	27.0	41.0
17	36.081780765433315	40.0	37.0	41.0	27.0	41.0
18	35.94607655846293	40.0	36.0	41.0	27.0	41.0
19	36.09031121972256	40.0	37.0	41.0	27.0	41.0
20	36.13516703423909	40.0	37.0	41.0	27.0	41.0
21	36.10672525127957	40.0	37.0	41.0	27.0	41.0
22	36.09851445259451	40.0	37.0	41.0	28.0	41.0
23	35.97605420148517	40.0	37.0	41.0	27.0	41.0
24	35.89642023552376	40.0	37.0	41.0	27.0	41.0
25	35.643619938943885	40.0	36.0	41.0	27.0	41.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.781629446039755
1101	2	0.6548149664525589
1101	3	0.7193517915464938
1101	4	0.8648538382729498
1101	5	0.8634796232270503
1101	6	1.0217107949045712
1101	7	0.833815758261018
1101	8	1.0523989154775961
1101	9	1.1851089560209758
1101	10	1.208200652196254
1101	11	0.9212864887034158
1101	12	1.1724006141942382
1101	13	0.9870349465488104
1101	14	1.2727800001238876
1101	15	1.140667008087462
1101	16	1.2031133158174612
1101	17	1.0996543403857686
1101	18	1.2518049872983141
1101	19	1.169098960275278
1101	20	1.2937293859551389
1101	21	1.3529619362176035
1101	22	1.4244714331997699
1101	23	1.37839237068264
1101	24	1.217241597866071
1101	25	1.48107809585985
1102	1	0.49083604258565927
1102	2	0.4045184681622942
1102	3	0.34206745617879264
1102	4	0.4580408702601346
1102	5	0.2636960453367294
1102	6	0.3104438598744892
1102	7	0.2824674485176928
1102	8	0.3359205360463591
1102	9	-0.02152221741970095
1102	10	0.2528874894035411
1102	11	0.23078741931848867
1102	12	0.24237421069685894
1102	13	0.14186332381583355
1102	14	0.1954913363139923
1102	15	0.22013720676295634
1102	16	0.31713314008271
1102	17	0.2519202798741489
1102	18	0.29018961267164656
1102	19	0.36625084531185337
1102	20	0.22082976252961117
1102	21	0.18685363859392368
1102	22	0.19566132195880925
1102	23	0.20849235769730967
1102	24	0.21629799746785494
1102	25	0.347848645139166
1103	1	0.5895799151346779
1103	2	0.5829140972472615
1103	3	0.5513709692506872
1103	4	0.6756930352928165
1103	5	0.6589421646177769
1103	6	0.4881756817699099
1103	7	0.5988992295634432
1103	8	0.5561258056541973
1103	9	0.698657521142394
1103	10	0.7204936985538666
1103	11	0.7434201807563952
1103	12	0.6756411633112336
1103	13	0.6006766408092972
1103	14	0.7536868020002672
1103	15	0.8097205400962935
1103	16	0.851150705442187
1103	17	0.6045091279133601
1103	18	0.6913538283579186
1103	19	0.9176244155732931
1103	20	0.6150852772354938
1103	21	0.744114668005686
1103	22	0.5939354232659966
1103	23	0.7596872106384893
1103	24	0.8210978340691568
1103	25	0.972618865727398
1104	1	0.8289301907507607
1104	2	0.9142664399839688
1104	3	0.9004204990820774
1104	4	0.8929484260191458
1104	5	0.9538361740211485
1104	6	0.8175723662705607
1104	7	0.7507413988241467
1104	8	0.8381712417761165
1104	9	1.0980576508440762
1104	10	1.124798173262036
1104	11	1.0082244122739041
1104	12	1.1301797090310828
1104	13	1.2828973768663658
1104	14	1.3931414469418968
1104	15	1.4893979071520604
1104	16	1.5781590549882267
1104	17	1.5522788668887202
1104	18	1.5692923821841163
1104	19	1.822683794626471
1104	20	1.5276734338502749
1104	21	1.4941855986152817
1104	22	1.3574629038107418
1104	23	1.5698870322986664
1104	24	1.4121119391275272
1104	25	1.587338993807812
1105	1	0.699822970690235
1105	2	0.7215267866411992
1105	3	0.7368981157153343
1105	4	0.584823401454436
1105	5	0.6272581368399983
1105	6	0.5433761236891073
1105	7	0.5566801639068828
1105	8	0.679429151613796
1105	9	0.748531258516131
1105	10	0.7921477389579081
1105	11	0.8776531353018484
1105	12	0.8190676153314413
1105	13	0.95918358525374
1105	14	0.8334295418992568
1105	15	0.8163493279750824
1105	16	0.908205629684609
1105	17	0.9035542668022529
1105	18	0.7596934748225621
1105	19	0.7262181655732931
1105	20	0.9494839514779159
1105	21	0.9618387841673055
1105	22	0.9202453980134706
1105	23	0.9936676399314166
1105	24	0.992025864372188
1105	25	0.8849847116869967
1106	1	0.31784362954003953
1106	2	0.3945700331769082
1106	3	0.37519058817866124
1106	4	0.4353703280063854
1106	5	0.2649605377333515
1106	6	0.318584234952489
1106	7	0.3212479523440166
1106	8	0.422456155319189
1106	9	0.3097337355477521
1106	10	0.5764924422724604
1106	11	0.5393137109071517
1106	12	0.47236567126768136
1106	13	0.5959786717975675
1106	14	0.7124585251995939
1106	15	0.8000367042503953
1106	16	0.7907052489932767
1106	17	0.6407122790858892
1106	18	0.6980278233327937
1106	19	0.7884040952215372
1106	20	0.6724358320931145
1106	21	0.6007022936338302
1106	22	0.7250205363313214
1106	23	0.5212423823302785
1106	24	0.7824933533321428
1106	25	0.7488241107022731
1107	1	0.19977172017772915
1107	2	0.14514022732106113
1107	3	0.22880985301452483
1107	4	-0.021036861693652043
1107	5	0.2572806870655029
1107	6	0.1541937091376866
1107	7	-0.11567065051035286
1107	8	-0.2398699277406351
1107	9	-0.03927298316265393
1107	10	0.2651200823177007
1107	11	0.3196328960085495
1107	12	0.43054679830508746
1107	13	0.4111106045239339
1107	14	0.5314149308440506
1107	15	0.5200142055329451
1107	16	0.4093937097472278
1107	17	0.4150343816033981
1107	18	0.35969254299506304
1107	19	0.4071837252288901
1107	20	0.17730179783819722
1107	21	0.2626513377473856
1107	22	0.17966151490191606
1107	23	0.14955306242200805
1107	24	0.23816809852180398
1107	25	0.31239534942973535
1108	1	0.4395881621911144
1108	2	0.3474495342143733
1108	3	0.433714021422837
1108	4	0.5488993005181513
1108	5	0.4548227599283763
1108	6	0.2044309471982544
1108	7	0.31742402148386617
1108	8	0.2452133640179852
1108	9	0.20766584432550417
1108	10	0.5061590456915042
1108	11	0.5910431253361779
1108	12	0.5671590657675907
1108	13	0.5501634201434413
1108	14	0.4727428946718035
1108	15	0.5789679010382329
1108	16	0.62002694884454
1108	17	0.49233488664255276
1108	18	0.6593615932787458
1108	19	0.42490878676695587
1108	20	0.6415123351932692
1108	21	0.4437283251311399
1108	22	0.4599619914754953
1108	23	0.5766564808374284
1108	24	0.49329002854743464
1108	25	0.4132638489795326
1109	1	-0.12686785056580518
1109	2	-0.18103140396046769
1109	3	-0.0460399607010018
1109	4	-0.279526620504285
1109	5	-0.09556795011652497
1109	6	-0.22764934842332707
1109	7	-0.23877438154766395
1109	8	-0.37124058927333436
1109	9	-0.3161371648479445
1109	10	0.030125341065946998
1109	11	-0.10406623228708156
1109	12	-0.354570944176686
1109	13	-0.02994684952886928
1109	14	-0.2639573707050502
1109	15	-0.19314781739163323
1109	16	-0.21591602161095835
1109	17	-0.08799162691755669
1109	18	-0.2786793842024693
1109	19	-0.1499895638953035
1109	20	-0.15772948725726366
1109	21	-0.33668998175276954
1109	22	-0.13964700427023757
1109	23	0.06558244010708592
1109	24	0.16220592585659688
1109	25	0.04989468094478866
1110	1	-0.23957858406113886
1110	2	-0.14051492114839448
1110	3	-0.28692881462546893
1110	4	-0.22268320867581792
1110	5	-0.1319061842965752
1110	6	-0.372356900156106
1110	7	-0.3012660046544866
1110	8	-0.3888364354998046
1110	9	-0.21240500398828743
1110	10	-0.1995530445100684
1110	11	-0.13313478003974666
1110	12	-0.08294253441695076
1110	13	-0.17112736502102166
1110	14	-0.30976659315454214
1110	15	-0.11231152665079236
1110	16	-0.028652395683671728
1110	17	-0.2798661862201328
1110	18	-0.09982907272772934
1110	19	-0.07158038689956925
1110	20	-0.14207853709904583
1110	21	0.16775195690797773
1110	22	0.08467162652293325
1110	23	0.07874945933168931
1110	24	0.1982527898391666
1110	25	0.4349841024584009
1111	1	0.07641027227753483
1111	2	0.22141856153297468
1111	3	0.028528707345927273
1111	4	-0.046814405183376095
1111	5	0.17289305747492278
1111	6	0.15145693176991415
1111	7	0.14971768194439505
1111	8	0.07275526993991122
1111	9	0.23377656876144215
1111	10	0.11721988903005354
1111	11	0.06477434742306087
1111	12	0.11466646093028032
1111	13	0.167120093190249
1111	14	0.15781626628597678
1111	15	0.07489911152486428
1111	16	0.22324891972790084
1111	17	0.2218082350562227
1111	18	0.2191812093102996
1111	19	0.3285991179542478
1111	20	0.21333676533073032
1111	21	0.1575819299104495
1111	22	0.34549792326599515
1111	23	0.2322318534956267
1111	24	0.00934188168820782
1111	25	0.2045013062035892
1112	1	-0.07043903523225836
1112	2	0.06761952163891749
1112	3	-0.15193810278484676
1112	4	-0.057844587824213534
1112	5	0.0013568879665584177
1112	6	-0.08823986235909587
1112	7	-0.40238323953659005
1112	8	-0.24324925469728953
1112	9	-0.11012482725467265
1112	10	-0.23304772670685026
1112	11	-0.1115099322215869
1112	12	-0.00775946193017063
1112	13	-0.25167646367120255
1112	14	-0.15984913127513067
1112	15	-0.08600416056033566
1112	16	-0.2107548324102666
1112	17	-0.19139873950648933
1112	18	-0.1299635239016439
1112	19	-0.18933803373145963
1112	20	0.04170541290563534
1112	21	-0.28757891542421277
1112	22	-0.28147194927166197
1112	23	-0.3943931534016798
1112	24	-0.13223236098684765
1112	25	-0.11421938021697997
1113	1	-0.4024497565824916
1113	2	-0.198927758813344
1113	3	-0.36916249034527127
1113	4	-0.2775503359193081
1113	5	-0.11670931546078833
1113	6	-0.5650694430897971
1113	7	-0.578561138001092
1113	8	-0.6809805004625247
1113	9	-0.518584341932808
1113	10	-0.6168028783260411
1113	11	-0.6429697603210442
1113	12	-0.4035754823116591
1113	13	-0.592724383320899
1113	14	-0.7044492459795322
1113	15	-0.4436113903189707
1113	16	-0.5950210818754371
1113	17	-0.5032919733773227
1113	18	-0.5516140448182867
1113	19	-0.577513596491805
1113	20	-0.38623434257371514
1113	21	-0.40684808451956656
1113	22	-0.4623303595622872
1113	23	-0.4791157495186411
1113	24	-0.3668405778275883
1113	25	-0.53813537809976
1114	1	-0.5117931909259283
1114	2	-0.3815035163891025
1114	3	-0.5338089549917342
1114	4	-0.2654291237980928
1114	5	-0.25746408538222454
1114	6	-0.5345279167149357
1114	7	-0.7091026643759477
1114	8	-0.4650152928306497
1114	9	-0.46649399400912017
1114	10	-0.3641653923552255
1114	11	-0.14166504651633005
1114	12	-0.19280107153724657
1114	13	-0.1481396470694918
1114	14	-0.20912096315124984
1114	15	-0.4741809750552264
1114	16	-0.2664155824366006
1114	17	-0.45200128875330137
1114	18	-0.40040753527844686
1114	19	-0.6851454707903457
1114	20	-0.6467281697342031
1114	21	-0.5861410138124938
1114	22	-0.7567748040067315
1114	23	-0.44054953178575573
1114	24	-0.7492620144156916
1114	25	-0.6359748842725992
1115	1	-0.4015255522137231
1115	2	-0.4421674756806162
1115	3	-0.37691493405035104
1115	4	-0.5233995710042763
1115	5	-0.45462501110880993
1115	6	-0.31938088667286024
1115	7	-0.4641361803755544
1115	8	-0.40491401090482526
1115	9	-0.47395191552786287
1115	10	-0.5433316512846673
1115	11	-0.369814816552541
1115	12	-0.38344594872679494
1115	13	-0.42596771243792375
1115	14	-0.4970686586204778
1115	15	-0.4177529977580292
1115	16	-0.6073087715940559
1115	17	-0.44155995398473635
1115	18	-0.6683911931706064
1115	19	-0.697759229475146
1115	20	-0.710540675850261
1115	21	-0.6634724552451203
1115	22	-0.597155467476739
1115	23	-0.5345668035344602
1115	24	-0.5125326377643518
1115	25	-0.7884775753382627
1116	1	-0.7860509768966253
1116	2	-0.7614904184944677
1116	3	-0.8304132045308847
1116	4	-0.7475868806623609
1116	5	-0.7642543735359055
1116	6	-0.9831659782265305
1116	7	-0.9621527759506066
1116	8	-1.0094244322504622
1116	9	-1.0190808281994777
1116	10	-1.0167995244984311
1116	11	-1.1079755636406148
1116	12	-1.0967110356393164
1116	13	-0.9522654838794296
1116	14	-1.2462061964878188
1116	15	-1.2076109255742864
1116	16	-1.096035015831383
1116	17	-1.191489015540924
1116	18	-1.3330091652386642
1116	19	-1.5041713755152841
1116	20	-1.348513364262196
1116	21	-1.7957315833283616
1116	22	-1.938306772571785
1116	23	-1.891957555458987
1116	24	-2.3700985817970803
1116	25	-2.4025437209855127
1201	1	0.4440203351706167
1201	2	0.5257312299965875
1201	3	0.5360758771572485
1201	4	0.5417749928399829
1201	5	0.6506558607094206
1201	6	0.5200328527043254
1201	7	0.4651040251609331
1201	8	0.47711286203335845
1201	9	0.6090910341702411
1201	10	0.5108407335942999
1201	11	0.6040375998938643
1201	12	0.5154077008224647
1201	13	0.24676968887757766
1201	14	0.8828387281008929
1201	15	0.779705939916596
1201	16	0.7587835109228678
1201	17	0.7967857732413037
1201	18	0.897932332401048
1201	19	0.7218381116649368
1201	20	0.7360456602094345
1201	21	0.828156952372261
1201	22	0.7787189475247587
1201	23	0.9662165794614879
1201	24	0.9520642536558626
1201	25	0.8941464095279343
1202	1	0.3162981910341145
1202	2	0.32390787975701585
1202	3	0.3845383673867957
1202	4	0.4392404588949006
1202	5	0.33010093412362806
1202	6	0.21421159150116154
1202	7	0.23785471829339855
1202	8	0.3226871542671148
1202	9	0.4788644999026843
1202	10	0.3900726972534798
1202	11	0.5393300474664073
1202	12	0.48857970633247305
1202	13	0.29681543036282676
1202	14	0.35837667250308414
1202	15	0.40011119896061587
1202	16	0.20378331814266204
1202	17	0.5020949401153558
1202	18	0.46677239142683646
1202	19	0.27254456349266576
1202	20	0.3513685320035904
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2210	18	-0.13276761292321737
2210	19	0.07660369344992546
2210	20	0.18701495546799407
2210	21	0.18375545982063102
2210	22	0.1989083621746559
2210	23	0.19219202973694394
2210	24	0.0498011258841089
2210	25	0.2166644988827926
2211	1	-0.4469810914364096
2211	2	-0.31568674654007367
2211	3	-0.27336581390343184
2211	4	-0.38214466632008737
2211	5	-0.26894855372152904
2211	6	-0.32282622235194935
2211	7	-0.45054260138039837
2211	8	-0.5711003368189154
2211	9	-0.8048520368026502
2211	10	-0.6516314507890257
2211	11	-0.48982541362831
2211	12	-0.5103420729492214
2211	13	-0.6779131889398116
2211	14	-0.5635781696245843
2211	15	-0.6529810728069378
2211	16	-0.6362578220369102
2211	17	-0.6406512290161501
2211	18	-0.617505938667172
2211	19	-0.7258427663263518
2211	20	-0.5749675528122964
2211	21	-0.6979309562475962
2211	22	-0.43398236347235297
2211	23	-0.49446638852518987
2211	24	-0.5511206545807781
2211	25	-0.5556582773430918
2212	1	-0.24870835665117497
2212	2	-0.4076385478289417
2212	3	-0.1708013881154713
2212	4	-0.14695955116673787
2212	5	-0.11562645349476952
2212	6	-0.2851687032437624
2212	7	-0.3245175103740294
2212	8	-0.4857469351625241
2212	9	-0.20963184970559468
2212	10	-0.17044270238400117
2212	11	-0.08934835675825781
2212	12	-0.24613156285132476
2212	13	-0.20810181268816308
2212	14	-0.19642646245851125
2212	15	-0.07669749429214079
2212	16	-0.18421772460861519
2212	17	-0.058214653348848344
2212	18	-0.1864487800828769
2212	19	-0.20153681684170976
2212	20	-0.21591712848940148
2212	21	-0.22700747443276725
2212	22	-0.3049417015874596
2212	23	-0.16432790757175297
2212	24	-0.05928146448496108
2212	25	-0.11542974945431439
2213	1	-0.5605896725439621
2213	2	-0.4445473381525069
2213	3	-0.486816434186931
2213	4	-0.5758051903734653
2213	5	-0.40652260218407577
2213	6	-0.4683093872721855
2213	7	-0.36414453733497254
2213	8	-0.14939062202057585
2213	9	-0.38474823397541513
2213	10	-0.5273698588041853
2213	11	-0.7592503725460347
2213	12	-0.7319773066578605
2213	13	-0.7499383408438547
2213	14	-0.5909035924570532
2213	15	-0.6248222370377405
2213	16	-0.8260503132909136
2213	17	-0.43473593533499866
2213	18	-0.5980979387336234
2213	19	-0.5300101078913642
2213	20	-0.7323386195393269
2213	21	-0.7174746280545676
2213	22	-0.8365125054709708
2213	23	-0.8057488892070097
2213	24	-0.9931825347991321
2213	25	-1.048170712777818
2214	1	-0.6540659181986541
2214	2	-0.7862004860860701
2214	3	-0.5373443085270893
2214	4	-0.8563382147071863
2214	5	-0.6910751964933368
2214	6	-0.8157652904523118
2214	7	-0.7087743815476628
2214	8	-0.5259748887902447
2214	9	-0.5688424788576114
2214	10	-0.7432563014461309
2214	11	-0.6585589859102683
2214	12	-0.667952586688763
2214	13	-0.5285941925240394
2214	14	-0.849802781333068
2214	15	-0.7520850154592651
2214	16	-0.5488145723355942
2214	17	-0.7342235109755251
2214	18	-0.8462156160865248
2214	19	-0.6454485010933766
2214	20	-0.5942029172089534
2214	21	-0.7487672764387554
2214	22	-0.8017242989562234
2214	23	-0.5689586226948435
2214	24	-0.39605494370862004
2214	25	-0.34653043982815745
2215	1	-1.1675141940607219
2215	2	-0.9818757734423933
2215	3	-0.9681153813240257
2215	4	-1.2296140767761514
2215	5	-1.1294468440029135
2215	6	-0.7990459417933096
2215	7	-0.7824764888273563
2215	8	-0.9312574558400541
2215	9	-0.9611844328806001
2215	10	-1.0068769911013078
2215	11	-1.0768923192436048
2215	12	-1.0647054602519859
2215	13	-1.2151459166619674
2215	14	-1.0458372640916878
2215	15	-1.1669317587542807
2215	16	-1.1931201278911487
2215	17	-1.5614840473739946
2215	18	-1.4417280681938038
2215	19	-1.674643903392223
2215	20	-1.5871866336840483
2215	21	-1.440960763028798
2215	22	-1.3982520767340034
2215	23	-1.9252086226948464
2215	24	-1.852573142942333
2215	25	-1.8521386773760469
2216	1	-1.4985268061479147
2216	2	-1.4290193726258877
2216	3	-1.5917370733849694
2216	4	-1.3466130561448182
2216	5	-1.262247383479476
2216	6	-1.373084999167368
2216	7	-1.2028923049679179
2216	8	-1.2192964767629988
2216	9	-1.3908861715989786
2216	10	-1.2104656037717163
2216	11	-1.3720825940850432
2216	12	-1.5826530799939107
2216	13	-1.498826750663575
2216	14	-1.744031111142796
2216	15	-1.7781714613131498
2216	16	-1.89191769660151
2216	17	-1.8031640794509798
2216	18	-2.2358726515575142
2216	19	-1.982819889394996
2216	20	-1.7549734128631727
2216	21	-2.0126737833685198
2216	22	-2.438487277579668
2216	23	-2.4291066142381865
2216	24	-2.438030513358605
2216	25	-2.5259854550971284
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	13145.0
3	0.0
4	93.0
5	764.0
6	1861.0
7	1789.0
8	1624.0
9	1336.0
10	1184.0
11	1654.0
12	1729.0
13	1508.0
14	714.0
15	571.0
16	631.0
17	682.0
18	797.0
19	1185.0
20	1590.0
21	2117.0
22	2503.0
23	2707.0
24	3018.0
25	3424.0
26	3754.0
27	4086.0
28	4422.0
29	5214.0
30	6310.0
31	8295.0
32	10716.0
33	14384.0
34	19854.0
35	28684.0
36	44573.0
37	86427.0
38	245349.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	53.89757796482345	11.494150506501333	14.076113526029962	20.53215800264525
2	6.589895229122444	22.50417153819086	41.30428202246766	29.60165121021904
3	23.694940203088137	33.558734549389925	25.46983287893609	17.276492368585856
4	14.221484650712163	12.446897689706525	30.22417926772269	43.10743839185862
5	18.736932924394964	30.913318814886015	27.09967210509287	23.25007615562615
6	40.11295264808474	25.227920955857996	11.874790626898553	22.7843357691587
7	25.042586858263366	33.80828721334312	21.179674901387763	19.969451027005746
8	26.094223198061783	34.36156117504543	25.97970926711084	13.56450635978195
9	30.649437653644608	24.33900883971	12.000317934335206	33.01123557231019
10	12.684875412516275	24.85353780011505	28.715863332223197	33.74572345514547
11	28.37346267105491	29.29362340413787	14.461028318641345	27.871885606165876
12	28.588737902489502	22.812937488587252	22.523053746424008	26.075270862499238
13	33.80254102397828	19.95241998208618	27.713363671458264	18.531675322477277
14	37.74894754655704	17.986400818384755	21.496100122833774	22.768551512224434
15	40.26433644895359	13.549620135210946	28.491285454839353	17.69475796099611
16	29.457753441326336	18.481443455776105	35.71583390371892	16.344969199178642
17	24.782913737800484	31.30719488613785	28.842859397311116	15.067031978750551
18	27.822743943827465	14.29886054024044	44.59597947337299	13.282416042559097
19	22.741500557184636	38.796024782355694	16.37875624217945	22.08371841828022
20	15.745200306511078	31.55447892165904	31.487168176087955	21.21315259574193
21	16.74384021936939	38.39781999768004	19.48424613640988	25.37409364654069
22	15.843140982983172	34.95313242703679	14.015590835630764	35.18813575434927
23	23.469560438933584	25.94697581910247	14.984474559383623	35.59898918258032
24	14.15201231915743	31.272029048877396	17.400999980988953	37.17495865097622
25	28.15800330425161	30.706345569964387	15.680746175300245	25.454904950483755
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	429.0
1	429.0
2	457.5
3	486.0
4	486.0
5	486.0
6	1204.0
7	1922.0
8	1922.0
9	1922.0
10	1776.0
11	1630.0
12	1630.0
13	1630.0
14	1554.5
15	1479.0
16	1479.0
17	1479.0
18	2286.0
19	3093.0
20	3093.0
21	3093.0
22	2834.5
23	2576.0
24	2576.0
25	2576.0
26	3527.0
27	4478.0
28	4478.0
29	4478.0
30	5216.5
31	5955.0
32	5955.0
33	5955.0
34	6075.5
35	6196.0
36	6196.0
37	6196.0
38	7649.5
39	9103.0
40	9103.0
41	9103.0
42	14339.5
43	19576.0
44	19576.0
45	19576.0
46	69186.5
47	118797.0
48	118797.0
49	118797.0
50	201077.0
51	283357.0
52	283357.0
53	283357.0
54	166239.5
55	49122.0
56	49122.0
57	49122.0
58	28898.5
59	8675.0
60	8675.0
61	8675.0
62	6743.5
63	4812.0
64	4812.0
65	4812.0
66	4003.5
67	3195.0
68	3195.0
69	3195.0
70	2531.0
71	1867.0
72	1867.0
73	1867.0
74	1491.5
75	1116.0
76	1116.0
77	1116.0
78	833.5
79	551.0
80	551.0
81	551.0
82	367.0
83	183.0
84	183.0
85	183.0
86	127.0
87	71.0
88	71.0
89	71.0
90	44.0
91	17.0
92	17.0
93	17.0
94	10.5
95	4.0
96	4.0
97	4.0
98	4.0
99	4.0
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.1808229334927198
2	0.02118427672718056
3	0.029317525827794528
4	0.044070861405652424
5	0.03234385107453461
6	0.06260710354193541
7	0.06847060870749432
8	0.07074035264254938
9	0.05352812780171517
10	0.043503425421888654
11	0.6338259938641255
12	0.5610050426144424
13	0.5377401672801281
14	0.5258240116210889
15	0.5388750392476555
16	0.5171233265367112
17	0.5203387971113725
18	0.5188256344880026
19	0.5364161499846792
20	0.5248782849814827
21	0.5339572607217029
22	0.5190147798159237
23	0.5263914476048527
24	0.5076660601406485
25	0.5116381120269948
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	528694.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	13.555667361460506
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.70352179494336	11.617684331579326
2	7.068705698498633	1.9164204624981558
3	2.2339119272199586	0.9084650100057878
4	1.1385834682145448	0.6173703503349763
5	0.6306859407266842	0.42746844110203636
6	0.44371267511302115	0.36088928567375456
7	0.2944131271976335	0.27936764933969366
8	0.25953005525478595	0.2814482479468275
9	0.21767036892336886	0.26556004040144204
>10	1.525087905341296	4.106723359826289
>50	0.17162471395881007	1.6045198167560064
>100	0.1758106825919518	5.334465683363155
>500	0.04185968633141709	4.07816241531018
>1k	0.07116146676340905	20.749053327633753
>5k	0.011162583021711223	10.253189935955392
>10k+	0.012557905899425128	37.19921164227322
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTACGGAAGCAGTGGTATCAACGCA	49195	9.305004407086141	No Hit
CTTCCGTACTCTGCGTTGATACCAC	29380	5.557089734326472	No Hit
GAGTACGGAAGCAGTGGTATCAACG	26444	5.0017590515496675	No Hit
TCCGTACTCTGCGTTGATACCACTG	25102	4.747926021479343	No Hit
GCTTCCGTACTCTGCGTTGATACCA	18708	3.538530794750839	No Hit
GTACATGGGAAGCAGTGGTATCAAC	12714	2.4047936991908365	No Hit
GCGTTGATACCACTGCTTCCGTACT	12211	2.309653599246445	No Hit
CCCATGTACTCTGCGTTGATACCAC	11832	2.237967519964289	No Hit
GTATCAACGCAGAGTACGGAAGCAG	11084	2.0964868146791904	No Hit
GTACATGGAAGCAGTGGTATCAACG	9953	1.8825634488002512	No Hit
TCCATGTACTCTGCGTTGATACCAC	8026	1.5180804018959928	No Hit
TATCAACGCAGAGTACGGAAGCAGT	7543	1.4267232085100268	No Hit
ACTCTGCGTTGATACCACTGCTTCC	7414	1.4023234612081847	No Hit
CAGTGGTATCAACGCAGAGTACGGA	5582	1.0558092204564455	No Hit
GGTATCAACGCAGAGTACGGAAGCA	5348	1.0115492137228719	No Hit
CATGTACTCTGCGTTGATACCACTG	5233	0.9897975010119275	No Hit
ATACCACTGCTTCCGTACTCTGCGT	5109	0.9663434803496919	No Hit
ACGCAGAGTACGGAAGCAGTGGTAT	4974	0.9408088610803225	No Hit
GAGTACATGGGAAGCAGTGGTATCA	4599	0.8698793631098519	No Hit
GTACGGGAAGCAGTGGTATCAACGC	3931	0.7435302840584535	No Hit
AAGCAGTGGTATCAACGCAGAGTAC	3812	0.7210219900358241	No Hit
GAAGCAGTGGTATCAACGCAGAGTA	3613	0.6833820697794943	No Hit
GAGTACATGGAAGCAGTGGTATCAA	3592	0.679410017893148	No Hit
GTGGTATCAACGCAGAGTACGGAAG	3543	0.6701418968250065	No Hit
GTATCAACGCAGAGTACATGGGAAG	3444	0.6514165093608023	No Hit
GTACTCTGCGTTGATACCACTGCTT	3370	0.6374197550946293	No Hit
GCGTTGATACCACTGCTTCCCATGT	3019	0.5710297449942688	No Hit
GTTGATACCACTGCTTCCGTACTCT	2919	0.5521152122021434	No Hit
CCCGTACTCTGCGTTGATACCACTG	2699	0.5105032400594672	No Hit
GATACCACTGCTTCCGTACTCTGCG	2688	0.5084226414523335	No Hit
CCACTGCTTCCGTACTCTGCGTTGA	2581	0.48818409136475915	No Hit
TTCCCGTACTCTGCGTTGATACCAC	2551	0.48250973152712157	No Hit
ATCAACGCAGAGTACGGAAGCAGTG	2482	0.469458703900555	No Hit
CGGAAGCAGTGGTATCAACGCAGAG	2320	0.43881716077731164	No Hit
GAGTACGGGAAGCAGTGGTATCAAC	2284	0.43200792897214646	No Hit
TATCAACGCAGAGTACATGGGAAGC	2280	0.43125134766046147	No Hit
CTGCTTCCGTACTCTGCGTTGATAC	2248	0.4251986971669812	No Hit
GCTTCCCATGTACTCTGCGTTGATA	2203	0.41668715741052476	No Hit
CAGTGGTATCAACGCAGAGTACATG	2051	0.38793706756649404	No Hit
GCTTCCATGTACTCTGCGTTGATAC	2001	0.3784798011704313	No Hit
CATGGAAGCAGTGGTATCAACGCAG	1996	0.37753407453082505	No Hit
CTTCCCGTACTCTGCGTTGATACCA	1934	0.3658070641997072	No Hit
GCGTTGATACCACTGCTTCCATGTA	1878	0.3552149258361169	No Hit
GTATCAACGCAGAGTACGGGAAGCA	1876	0.3548366351802744	No Hit
ACGCAGAGTACATGGGAAGCAGTGG	1873	0.3542691991965107	No Hit
AAAAAGTACTCTGCGTTGATACCAC	1813	0.34292047952123533	No Hit
GTATCAACGCAGAGTACATGGAAGC	1703	0.3221144934498973	No Hit
GTATCAACGCAGAGTACTTTTTTTT	1565	0.2960124381967641	No Hit
GCAGAGTACGGAAGCAGTGGTATCA	1542	0.29166209565457524	No Hit
GCAGTGGTATCAACGCAGAGTACGG	1541	0.291472950326654	No Hit
GCGTTGATACCACTGCTTCCCGTAC	1489	0.28163739327474874	No Hit
TGATACCACTGCTTCCGTACTCTGC	1488	0.2814482479468275	No Hit
CACTGCTTCCGTACTCTGCGTTGAT	1474	0.2788002133559299	No Hit
GGTATCAACGCAGAGTACATGGGAA	1427	0.2699103829436309	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	1369	0.2589399539241981	No Hit
AAAGTACTCTGCGTTGATACCACTG	1365	0.2581833726125131	No Hit
TACCACTGCTTCCGTACTCTGCGTT	1341	0.25364388474240296	No Hit
CTGCGTTGATACCACTGCTTCCGTA	1323	0.2502392688398204	No Hit
GCCCATGTACTCTGCGTTGATACCA	1311	0.24796952490476531	No Hit
CCATGTACTCTGCGTTGATACCACT	1302	0.24626721695347403	No Hit
TATCAACGCAGAGTACATGGAAGCA	1147	0.21694969112567952	No Hit
GCTTCCCGTACTCTGCGTTGATACC	1142	0.21600396448607323	No Hit
CCCCATGTACTCTGCGTTGATACCA	1139	0.21543652850230946	No Hit
ATACCACTGCTTCCCATGTACTCTG	1138	0.21524738317438818	No Hit
GGGAAGCAGTGGTATCAACGCAGAG	1128	0.21335592989517568	No Hit
ACGCAGAGTACATGGAAGCAGTGGT	1099	0.20787071538545926	No Hit
TATCAACGCAGAGTACGGGAAGCAG	1090	0.20616840743416798	No Hit
TATCAACGCAGAGTACTTTTTTTTT	1002	0.1895236185770975	No Hit
CTGCTTCCCATGTACTCTGCGTTGA	985	0.1863081480024362	No Hit
TACCACTGCTTCCCATGTACTCTGC	960	0.18157951480440482	No Hit
TACGGAAGCAGTGGTATCAACGCAG	924	0.17477028299923963	No Hit
GTGGTATCAACGCAGAGTACATGGG	906	0.17136566709665704	No Hit
GCAGTGGTATCAACGCAGAGTACAT	899	0.17004164980120828	No Hit
CTCTGCGTTGATACCACTGCTTCCG	895	0.16928506848952324	No Hit
TCAACGCAGAGTACGGAAGCAGTGG	895	0.16928506848952324	No Hit
CATGGGAAGCAGTGGTATCAACGCA	879	0.16625874324278317	No Hit
GGTATCAACGCAGAGTACGGGAAGC	841	0.15907122078177546	No Hit
ACGGAAGCAGTGGTATCAACGCAGA	797	0.15074882635324025	No Hit
GCAGAGTACATGGGAAGCAGTGGTA	782	0.14791164643442142	No Hit
ATACCACTGCTTCCATGTACTCTGC	778	0.1471550651227364	No Hit
AGCAGTGGTATCAACGCAGAGTACG	758	0.1433721585643113	No Hit
GGTATCAACGCAGAGTACATGGAAG	753	0.14242643192470503	No Hit
TCCCATGTACTCTGCGTTGATACCA	751	0.1420481412688625	No Hit
CTTCCCATGTACTCTGCGTTGATAC	700	0.1324017295448785	No Hit
GTTGATACCACTGCTTCCCATGTAC	683	0.12918625897021718	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	683	0.12918625897021718	No Hit
GGTATCAACGCAGAGTACTTTTTTT	664	0.12559249773971334	No Hit
CCACTGCTTCCCATGTACTCTGCGT	640	0.12105300986960321	No Hit
TTGATACCACTGCTTCCGTACTCTG	571	0.10800198224303661	No Hit
GATACCACTGCTTCCCATGTACTCT	570	0.10781283691511537	No Hit
ACATGGGAAGCAGTGGTATCAACGC	548	0.10365163970084774	No Hit
ATCAACGCAGAGTACATGGGAAGCA	548	0.10365163970084774	No Hit
CAACGCAGAGTACGGAAGCAGTGGT	541	0.10232762240539896	No Hit
GTGGTATCAACGCAGAGTACATGGA	533	0.10081445978202892	No Hit
CCGTACTCTGCGTTGATACCACTGC	533	0.10081445978202892	No Hit
AACGCAGAGTACGGAAGCAGTGGTA	530	0.10024702379826517	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	3.7829065584251006E-4
8	0.0	0.0	0.0	0.0	3.7829065584251006E-4
9	0.0	0.0	0.0	1.8914532792125503E-4	3.7829065584251006E-4
10	0.0	0.0	0.0	5.67435983763765E-4	3.7829065584251006E-4
11	0.0	0.0	0.0	7.565813116850201E-4	5.67435983763765E-4
12	0.0	0.0	0.0	0.0020805986071338054	5.67435983763765E-4
13	0.0	0.0	0.0	0.0030263252467400805	5.67435983763765E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CATGGTA	50	4.725871E-6	17.032354	4
ACATGGT	50	8.995594E-5	15.139871	3
ACCCCGT	45	6.8882236E-4	14.738852	1
CCCCGTA	100	1.0586518E-8	13.247387	2
AAAAAGT	370	0.0	13.060102	1
CCCCATG	170	0.0	12.819086	1
GCCCATG	250	0.0	12.5069685	1
AAAAGTA	410	0.0	12.231909	2
ATGGTAA	55	0.0030329456	12.107261	5
ACATGGA	2270	0.0	10.254428	3
TGGGCAG	105	4.029882E-5	9.96584	6
AGCAGAG	145	1.5748992E-7	9.840877	8
CATGGAA	2390	0.0	9.69997	4
ATGGAAG	2385	0.0	9.572676	5
TGGAAGC	2400	0.0	9.5524845	6
ATGGAAA	80	0.004457089	9.512848	5
GCTCCCG	90	0.00113807	9.474977	1
TCCATGT	1680	0.0	9.30578	1
CCATGTA	4600	0.0	9.174433	2
TACATGG	6760	0.0	9.056488	2
>>END_MODULE