FastQCFastQC Report
Thu 2 Feb 2017
SRR4050226_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4050226_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences528694
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACGGAAGCAGTGGTATCAACGCA448538.48373539325205No Hit
CTTCCGTACTCTGCGTTGATACCAC281145.3176317491781635No Hit
GAGTACGGAAGCAGTGGTATCAACG269455.096520860838217No Hit
TCCGTACTCTGCGTTGATACCACTG254394.811667996988806No Hit
GCTTCCGTACTCTGCGTTGATACCA181803.438662061608416No Hit
CCCATGTACTCTGCGTTGATACCAC130262.463807041502268No Hit
GCGTTGATACCACTGCTTCCGTACT117792.227942817584463No Hit
GTATCAACGCAGAGTACGGAAGCAG111432.1076463890265447No Hit
GTACATGGGAAGCAGTGGTATCAAC108592.053929115896908No Hit
TCCATGTACTCTGCGTTGATACCAC90711.715737269573704No Hit
TATCAACGCAGAGTACGGAAGCAGT86521.6364853771746986No Hit
GTACATGGAAGCAGTGGTATCAACG83091.571608529697708No Hit
ACTCTGCGTTGATACCACTGCTTCC77761.4707940699156792No Hit
CAGTGGTATCAACGCAGAGTACGGA65061.2305795034556852No Hit
ACGCAGAGTACGGAAGCAGTGGTAT59391.1233341025243335No Hit
CATGTACTCTGCGTTGATACCACTG57781.0928817047290116No Hit
GGTATCAACGCAGAGTACGGAAGCA54851.0374621236480837No Hit
ATACCACTGCTTCCGTACTCTGCGT51810.9799619439600223No Hit
AAGCAGTGGTATCAACGCAGAGTAC44790.8471819237593011No Hit
GAGTACATGGGAAGCAGTGGTATCA41990.7942212319413497No Hit
GTGGTATCAACGCAGAGTACGGAAG40770.7711455019349567No Hit
GAAGCAGTGGTATCAACGCAGAGTA38500.7282095124968319No Hit
GTACGGGAAGCAGTGGTATCAACGC36290.6864083950262344No Hit
GAGTACATGGAAGCAGTGGTATCAA33420.6321236859128342No Hit
GTATCAACGCAGAGTACATGGGAAG33080.6256927447635117No Hit
GCGTTGATACCACTGCTTCCCATGT31840.602238724101276No Hit
CCCGTACTCTGCGTTGATACCACTG29250.5532500841696709No Hit
ATCAACGCAGAGTACGGAAGCAGTG28350.536227004656758No Hit
GATACCACTGCTTCCGTACTCTGCG28340.5360378593288367No Hit
CCACTGCTTCCGTACTCTGCGTTGA27960.528850336867829No Hit
GTTGATACCACTGCTTCCGTACTCT27300.5163667452250262No Hit
GTACTCTGCGTTGATACCACTGCTT26990.5105032400594672No Hit
CGGAAGCAGTGGTATCAACGCAGAG26360.4985870844004282No Hit
TTCCCGTACTCTGCGTTGATACCAC26100.4936693058744756No Hit
TATCAACGCAGAGTACATGGGAAGC24870.47040443054016123No Hit
GTATCAACGCAGAGTACTTTTTTTT23620.4467612645500043No Hit
GAGTACGGGAAGCAGTGGTATCAAC23490.444302375287028No Hit
GCTTCCCATGTACTCTGCGTTGATA23090.43673656217017787No Hit
CATGGAAGCAGTGGTATCAACGCAG22960.4342776729072015No Hit
CTGCTTCCGTACTCTGCGTTGATAC21610.4087430536378321No Hit
CAGTGGTATCAACGCAGAGTACATG21170.4004206592092969No Hit
GTACTTTTTTTTTTTTTTTTTTTTT21140.3998532232255331No Hit
ACGCAGAGTACATGGGAAGCAGTGG20820.393800572732053No Hit
GCTTCCATGTACTCTGCGTTGATAC20400.3858564689593602No Hit
AAAAAGTACTCTGCGTTGATACCAC19800.3745077492840849No Hit
GTATCAACGCAGAGTACGGGAAGCA19400.3669419361672347No Hit
GCAGTGGTATCAACGCAGAGTACGG18400.34802740337510923No Hit
GCGTTGATACCACTGCTTCCATGTA18120.3427313341933141No Hit
CTTCCCGTACTCTGCGTTGATACCA18110.34254218886539284No Hit
GCAGAGTACGGAAGCAGTGGTATCA18080.34197475288162904No Hit
TATCAACGCAGAGTACTTTTTTTTT17370.3285454345992199No Hit
GTATCAACGCAGAGTACATGGAAGC16300.3083068845116457No Hit
CACTGCTTCCGTACTCTGCGTTGAT15730.29752560082013413No Hit
GCGTTGATACCACTGCTTCCCGTAC14980.28333970122604No Hit
TGATACCACTGCTTCCGTACTCTGC14760.2791785040117724No Hit
CCATGTACTCTGCGTTGATACCACT14340.2712344002390797No Hit
AAAGTACTCTGCGTTGATACCACTG14270.2699103829436309No Hit
GGTATCAACGCAGAGTACATGGGAA13960.264046877778072No Hit
TACCACTGCTTCCGTACTCTGCGTT13460.25458961138200925No Hit
CTGCGTTGATACCACTGCTTCCGTA13450.254400466054088No Hit
GCCCATGTACTCTGCGTTGATACCA13080.24740208892100155No Hit
TATCAACGCAGAGTACATGGAAGCA12830.2426734557229702No Hit
TATCAACGCAGAGTACGGGAAGCAG12740.2409711477716789No Hit
GGGAAGCAGTGGTATCAACGCAGAG12390.23435106129443498No Hit
CCCCATGTACTCTGCGTTGATACCA12340.2334053346548287No Hit
TACGGAAGCAGTGGTATCAACGCAG11500.21751712710944326No Hit
GAGTACTTTTTTTTTTTTTTTTTTT11260.21297763923933316No Hit
ATACCACTGCTTCCCATGTACTCTG11050.20900558735298677No Hit
GCTTCCCGTACTCTGCGTTGATACC10980.20768157005753798No Hit
TCAACGCAGAGTACGGAAGCAGTGG10840.20503353546664044No Hit
ACGCAGAGTACATGGAAGCAGTGGT10820.20465524481079791No Hit
CTGCTTCCCATGTACTCTGCGTTGA10470.198035158333554No Hit
CATGGGAAGCAGTGGTATCAACGCA10270.1942522517751289No Hit
TACCACTGCTTCCCATGTACTCTGC9650.1825252414440111No Hit
GGTATCAACGCAGAGTACTTTTTTT9450.178742334885586No Hit
ACGGAAGCAGTGGTATCAACGCAGA9380.1774183175901372No Hit
GCAGTGGTATCAACGCAGAGTACAT9380.1774183175901372No Hit
ATACCACTGCTTCCATGTACTCTGC9280.17552686431092465No Hit
CTCTGCGTTGATACCACTGCTTCCG8890.1681501965219957No Hit
ACGCAGAGTACTTTTTTTTTTTTTT8610.16285412734020058No Hit
GTGGTATCAACGCAGAGTACATGGG8440.15963865676553923No Hit
GGTATCAACGCAGAGTACGGGAAGC8170.15453173291166536No Hit
AGCAGTGGTATCAACGCAGAGTACG8140.1539642969279016No Hit
GCAGAGTACATGGGAAGCAGTGGTA8100.15320771561621657No Hit
TCCCATGTACTCTGCGTTGATACCA7900.14942480905779146No Hit
ACATGGGAAGCAGTGGTATCAACGC7000.1324017295448785No Hit
GGTATCAACGCAGAGTACATGGAAG6870.1299428402819022No Hit
CCACTGCTTCCCATGTACTCTGCGT6780.1282405323306109No Hit
CTTCCCATGTACTCTGCGTTGATAC6730.12729480569100463No Hit
GTTGATACCACTGCTTCCCATGTAC6710.12691651503516213No Hit
AACGCAGAGTACGGAAGCAGTGGTA6530.12351189913257953No Hit
CAACGCAGAGTACGGAAGCAGTGGT6420.12143130052544573No Hit
GATACCACTGCTTCCCATGTACTCT6210.11745924863909936No Hit
TTGATACCACTGCTTCCGTACTCTG5960.112730615441068No Hit
CCACTGCTTCCATGTACTCTGCGTT5710.10800198224303661No Hit
GGAAGCAGTGGTATCAACGCAGAGT5670.1072454009313516No Hit
ATCAACGCAGAGTACATGGGAAGCA5610.10611052896382406No Hit
CAGTGGTATCAACGCAGAGTACGGG5480.10365163970084774No Hit
AGTGGTATCAACGCAGAGTACGGAA5300.10024702379826517No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCCGT250.00592853919.0542071
GTTATCA552.737761E-819.0542051
GTCCGTA250.00598075319.0253092
TTATCAA607.5409844E-817.4398652
TACATGA402.7289317E-416.6471442
TACGAAA402.7289317E-416.6471442
CGAAAGC402.7289317E-416.6471444
ACGAAAG350.002148457316.3074053
AGGCAAA350.002174442216.2780911
GGACGGA704.2795E-714.9711611
TACGGAG456.684062E-414.79746152
GACGGAA653.315814E-614.6348522
GCCCATG2850.014.039941
ACAAAGA500.00150255613.2937758
GTACGAA655.2956893E-513.1913731
AAAAAGT3550.012.6133461
CCCCATG3200.012.5043211
GAGTATG550.002999134812.1254031
AGTATGG550.003033434712.1070152
AAAAGTA3800.011.7656512
Produced by FastQC (version 0.11.5)