##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4050226_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	528694
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.778531626990283	31.0	31.0	34.0	27.0	34.0
2	30.525549750895603	31.0	31.0	34.0	26.0	34.0
3	30.960998233382636	31.0	31.0	34.0	27.0	34.0
4	34.41147052926646	37.0	35.0	37.0	31.0	37.0
5	34.35320052809376	37.0	35.0	37.0	31.0	37.0
6	34.385094213287836	37.0	35.0	37.0	31.0	37.0
7	34.211585529625836	37.0	35.0	37.0	30.0	37.0
8	34.24620669044854	37.0	35.0	37.0	31.0	37.0
9	35.799751841329766	39.0	37.0	39.0	30.0	39.0
10	35.805537796910876	39.0	37.0	39.0	30.0	39.0
11	35.7861182460932	39.0	37.0	39.0	30.0	39.0
12	35.785444888725806	39.0	37.0	39.0	30.0	39.0
13	35.60261323185056	39.0	37.0	39.0	30.0	39.0
14	36.77905177664206	40.0	37.0	41.0	31.0	41.0
15	36.72041672498648	40.0	37.0	41.0	31.0	41.0
16	36.426719425603466	39.0	37.0	41.0	30.0	41.0
17	36.53374541795443	40.0	37.0	41.0	30.0	41.0
18	36.44576636012514	40.0	37.0	41.0	30.0	41.0
19	36.62288582809716	40.0	37.0	41.0	30.0	41.0
20	36.65768289407483	40.0	37.0	41.0	30.0	41.0
21	36.6647191002735	40.0	37.0	41.0	30.0	41.0
22	36.667242298947976	40.0	37.0	41.0	30.0	41.0
23	36.581364645711886	40.0	37.0	41.0	30.0	41.0
24	36.49433698888204	40.0	37.0	41.0	30.0	41.0
25	36.21978308813794	40.0	37.0	41.0	30.0	41.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.21670537976161697
1101	2	0.6417168689479844
1101	3	0.39832505618846525
1101	4	0.40467965095879777
1101	5	0.3063662509114735
1101	6	0.17900908841333063
1101	7	0.24258362753475637
1101	8	0.23287417439874503
1101	9	0.16369693217158954
1101	10	0.33554877333885
1101	11	0.24372626976366263
1101	12	0.43774299404568495
1101	13	0.28829062980162234
1101	14	0.2554063445486534
1101	15	0.1608036942570834
1101	16	0.18728067396621384
1101	17	0.1873724663375924
1101	18	0.2738227260289676
1101	19	0.24346383677882244
1101	20	0.23732817091274683
1101	21	-0.17872490814010433
1101	22	0.30023971133474703
1101	23	0.32419684613838484
1101	24	0.23553692766151357
1101	25	0.25903554066827894
1102	1	0.386241803602676
1102	2	0.6884294171019043
1102	3	0.5591439974515069
1102	4	0.39848132173748496
1102	5	0.4867843784273802
1102	6	0.26144859843367385
1102	7	0.20335928437586404
1102	8	0.24953001995992707
1102	9	0.20230663653899228
1102	10	0.47144454445082573
1102	11	0.32608786782452626
1102	12	0.4380632987680144
1102	13	0.5725221148901412
1102	14	0.40575911258743247
1102	15	0.5430051399567759
1102	16	0.4422951742475618
1102	17	0.5705521399730529
1102	18	0.48684268014748255
1102	19	0.4507789040860999
1102	20	0.8651600542179452
1102	21	0.7539764726329565
1102	22	0.6658199390108095
1102	23	0.7050911022955901
1102	24	0.5979573925362089
1102	25	0.3211659999159906
1103	1	0.20837722026934458
1103	2	0.5675705527228203
1103	3	0.23534906281098245
1103	4	0.10342413219500202
1103	5	-0.05050116078830058
1103	6	0.10776702163628471
1103	7	0.1195255425457944
1103	8	0.06511927649587079
1103	9	0.23713098294421542
1103	10	-0.0767580699282604
1103	11	0.1808468547526303
1103	12	0.11146505530395956
1103	13	-0.08596951092685146
1103	14	0.18213268284887363
1103	15	0.14452679028357096
1103	16	-0.018432971177269053
1103	17	0.02431541611684196
1103	18	-0.04577578390480852
1103	19	-0.01464184754789244
1103	20	0.24954016042709526
1103	21	0.2013161376656356
1103	22	0.3009812952199553
1103	23	0.1295345254982081
1103	24	0.28231707390875727
1103	25	0.32529692311860714
1104	1	0.4287839566506655
1104	2	0.7719064395580979
1104	3	0.6261361899186362
1104	4	0.48381485852444683
1104	5	0.5266409436215511
1104	6	0.3534655868113816
1104	7	0.2646191709504677
1104	8	0.3075651665736956
1104	9	0.424732312386503
1104	10	0.3961667030937903
1104	11	0.3713664721326566
1104	12	0.4333228292987741
1104	13	0.3332962559343713
1104	14	0.5374190398527645
1104	15	0.44435047705400166
1104	16	0.5451765862157245
1104	17	0.6483477199430467
1104	18	0.5787986324376035
1104	19	0.5754400266414734
1104	20	0.7476604992727545
1104	21	0.82027183643347
1104	22	0.7003307602005009
1104	23	0.59088240973945
1104	24	0.6111564464768477
1104	25	0.6977484876451925
1105	1	0.3301811975420712
1105	2	0.6728025602985781
1105	3	0.3999639618008821
1105	4	0.5342953443162131
1105	5	0.41390856143392085
1105	6	0.2889144332524509
1105	7	0.4654062243639814
1105	8	0.4494706022534487
1105	9	0.25875345769168945
1105	10	0.6088479906778019
1105	11	0.4777218547526303
1105	12	0.5321958002534544
1105	13	0.7183628754367817
1105	14	0.6018375439599808
1105	15	0.37850721957649824
1105	16	0.6259404505399004
1105	17	0.6594558832885582
1105	18	0.9408088120547902
1105	19	0.6818543645733186
1105	20	0.690709668002853
1105	21	0.6782337513020025
1105	22	0.5979352093613741
1105	23	0.5961933007507341
1105	24	0.6311570360299683
1105	25	0.6741368852398182
1106	1	-0.14918533207571372
1106	2	0.26572931528563615
1106	3	-0.050533474877461515
1106	4	0.16492957608110004
1106	5	0.04051380989846365
1106	6	0.02909894165303939
1106	7	-0.217877619095745
1106	8	0.12413178696488814
1106	9	0.07965087406649474
1106	10	0.08006772571663134
1106	11	-0.18098836300281818
1106	12	0.19239705907280324
1106	13	0.15863240490229202
1106	14	0.2013629487617692
1106	15	0.19921429028357096
1106	16	0.1868447193587386
1106	17	0.262426544676309
1106	18	0.20979896483891025
1106	19	0.1887448777452434
1106	20	0.05792453019259369
1106	21	0.19127242954168366
1106	22	0.022152254181435183
1106	23	0.07743156276788454
1106	24	0.0725115358015529
1106	25	0.1228196932191068
1107	1	0.018960130785949048
1107	2	0.1778344287990805
1107	3	0.1621056741270941
1107	4	-0.01807802032652006
1107	5	-0.0521635992876881
1107	6	0.0124458731122985
1107	7	0.1232251889172602
1107	8	-0.12321194275381941
1107	9	-0.07823263944200676
1107	10	-0.19437799612752116
1107	11	-0.2565954884208068
1107	12	-0.1833871510306011
1107	13	-0.0677547584668261
1107	14	-0.13657984021386227
1107	15	-0.04678816974102773
1107	16	-0.013335146761527028
1107	17	-0.23160882281304396
1107	18	-0.5080105625025979
1107	19	-0.2934894797742089
1107	20	-0.13943681528015617
1107	21	-0.29656497395797743
1107	22	-0.2666982097000883
1107	23	-0.4172203561131127
1107	24	-0.4864069805192912
1107	25	-0.2842942899810268
1108	1	0.30439040287186003
1108	2	0.7733266809201389
1108	3	0.5490861212762681
1108	4	0.5439057602908193
1108	5	0.7216294979611888
1108	6	0.5551809350548851
1108	7	0.38260347088522195
1108	8	0.3806151783432128
1108	9	0.5996779277270008
1108	10	0.6248761160238345
1108	11	0.4525493009564414
1108	12	0.4472645757059084
1108	13	0.6876630636772916
1108	14	0.5447256208310094
1108	15	0.7302739735966028
1108	16	0.5689715532847686
1108	17	0.6612752339203354
1108	18	0.44183459977363526
1108	19	0.5783405418146756
1108	20	0.6635439794486189
1108	21	0.6103241436542675
1108	22	0.5302209166166278
1108	23	0.5380033008737612
1108	24	0.5240398839980784
1108	25	0.34417158054856856
1109	1	0.06909204514856881
1109	2	0.4599127930609832
1109	3	0.22198855556460728
1109	4	0.41456543654282996
1109	5	0.43081677399430873
1109	6	0.31993112187783623
1109	7	0.34820156911584377
1109	8	0.5065632680417664
1109	9	0.3733779636205483
1109	10	0.45162357258382
1109	11	0.44511315910045113
1109	12	0.41224630530395956
1109	13	0.41895576081228114
1109	14	0.47019503188268885
1109	15	0.36708868641883896
1109	16	0.2966145831705518
1109	17	0.15061749945017766
1109	18	0.5435207136797331
1109	19	0.2873660026936591
1109	20	0.3401387413449726
1109	21	0.49998386109558624
1109	22	0.5690594957030513
1109	23	0.5944915001358879
1109	24	0.614084133087502
1109	25	0.6552523880944534
1110	1	-0.04415626636113501
1110	2	0.20774791249362679
1110	3	-0.07984620256899433
1110	4	0.014222785190582954
1110	5	0.16203213736208255
1110	6	0.04160265442679645
1110	7	-0.0602499148325677
1110	8	1.7060590882067572E-4
1110	9	-0.24454246900592125
1110	10	-0.054190719707108315
1110	11	-0.10169109257346776
1110	12	-0.04523258492523041
1110	13	0.00751190493563314
1110	14	0.09949201387018292
1110	15	-0.07099077606142146
1110	16	-0.23993635125366808
1110	17	-0.013134008390593976
1110	18	-0.07056091659479335
1110	19	0.04974923608700976
1110	20	0.12050825702942802
1110	21	0.14868464138498183
1110	22	0.08377369067633111
1110	23	0.1804602769055208
1110	24	0.20376642302415604
1110	25	0.05700964175552059
1111	1	0.11838466074553367
1111	2	0.2902268027228203
1111	3	0.1378788247157452
1111	4	0.2590788940997655
1111	5	-0.005932708407350162
1111	6	0.28086970020771673
1111	7	0.278826137783895
1111	8	0.0820463598292065
1111	9	-0.05051782657959336
1111	10	0.14109907292888835
1111	11	0.03367423570500705
1111	12	0.06700821006586466
1111	13	0.2819992390731514
1111	14	0.3073930995155365
1111	15	0.18254762361690524
1111	16	0.2970804216798726
1111	17	0.20656988040256152
1111	18	0.16270635895233454
1111	19	0.028066485785444684
1111	20	0.27361010090329074
1111	21	0.34316881623706763
1111	22	0.08617474760090715
1111	23	0.15189315645058343
1111	24	0.19760725248018218
1111	25	0.06677757788051508
1112	1	-0.10112783718527396
1112	2	0.1672546126880583
1112	3	0.031101525151655096
1112	4	0.12726296958395977
1112	5	0.0521537011274944
1112	6	-0.09455379479862813
1112	7	0.048989742043673346
1112	8	0.22037196817219495
1112	9	0.22510293157303352
1112	10	0.05278036189870505
1112	11	0.11078481342392621
1112	12	0.17499253937116777
1112	13	0.18947316723073726
1112	14	0.16782183647961801
1112	15	0.10419690905529677
1112	16	0.15753536843261884
1112	17	0.2986476269125191
1112	18	0.1477171187603119
1112	19	0.25208154951661044
1112	20	0.05905125303041814
1112	21	0.22078703617857798
1112	22	0.16168101036093674
1112	23	0.13085773185201077
1112	24	0.2706677281188732
1112	25	0.3226761597929979
1113	1	-0.4209411369135978
1113	2	-0.27613122196853723
1113	3	-0.4064333445964259
1113	4	-0.04525011022924019
1113	5	-0.114430383571694
1113	6	-0.3874323680943519
1113	7	-0.36245572849797725
1113	8	-0.43212311267360093
1113	9	-0.43709390482233346
1113	10	-0.5609050957419512
1113	11	-0.6008415571441148
1113	12	-0.5021756924513738
1113	13	-0.42782118741394726
1113	14	-0.4351994930770573
1113	15	-0.4461280217254071
1113	16	-0.6735825573164433
1113	17	-0.5286754298427496
1113	18	-0.5290284494491431
1113	19	-0.44216359277796613
1113	20	-0.5988526215594376
1113	21	-0.6159581678899144
1113	22	-0.3669918388990112
1113	23	-0.3119155656914714
1113	24	-0.3092189460126846
1113	25	-0.2285006007310031
1114	1	-0.6785309236700492
1114	2	-0.8688641063680898
1114	3	-0.6012986644617442
1114	4	-0.5547198071989357
1114	5	-0.5984651759398147
1114	6	-0.39618657684856373
1114	7	-0.5683058968481447
1114	8	-0.5190900038071575
1114	9	-0.5542515928133582
1114	10	-0.47751564568583404
1114	11	-0.43515693312615866
1114	12	-0.5468446037869512
1114	13	-0.6532280336541234
1114	14	-0.6596523550299196
1114	15	-0.6564675278982506
1114	16	-0.6234282363287846
1114	17	-0.8937006823680065
1114	18	-0.6494689657229884
1114	19	-0.5207971505781899
1114	20	-0.5028509380577475
1114	21	-0.40547836991012076
1114	22	-0.34764559871943845
1114	23	-0.24534710328967435
1114	24	-0.5279338730609453
1114	25	-0.5152570541541195
1115	1	-0.3907226743305863
1115	2	-0.47928342332669516
1115	3	-0.30688384892206244
1115	4	-0.2046916733306503
1115	5	-0.23125948066630997
1115	6	-0.4197505263543846
1115	7	-0.225785836176847
1115	8	-0.2918772139059911
1115	9	-0.39611601203245783
1115	10	-0.44646564079298656
1115	11	-0.45948661313398986
1115	12	-0.4093360413052949
1115	13	-0.3020158308945611
1115	14	-0.2796359639936128
1115	15	-0.27979539755281024
1115	16	-0.35556322996808376
1115	17	-0.37763869000084327
1115	18	-0.6420991154440969
1115	19	-0.5074824916096077
1115	20	-0.374006383796079
1115	21	-0.4149056622087812
1115	22	-0.3604293838256112
1115	23	-0.40057877957893595
1115	24	-0.3767571434396473
1115	25	-0.450524692866324
1116	1	-0.6707485912745916
1116	2	-0.9892555878855056
1116	3	-0.5251732157375777
1116	4	-0.370239599573047
1116	5	-0.2027462470565382
1116	6	-0.43144416877638037
1116	7	-0.33171789840048405
1116	8	-0.5522919539381377
1116	9	-0.5977087093361106
1116	10	-0.45753893083185204
1116	11	-0.6933916279937264
1116	12	-0.7310301087835569
1116	13	-0.7576485730933413
1116	14	-0.7892451075282167
1116	15	-0.6801880576353199
1116	16	-0.765582839996199
1116	17	-1.0721679861421407
1116	18	-0.9154229290488871
1116	19	-1.0918107820907608
1116	20	-0.9779297679791625
1116	21	-0.8764822992785071
1116	22	-1.2323392535171465
1116	23	-1.343902696575789
1116	24	-1.412296442188719
1116	25	-1.6465755755473452
1201	1	0.40996148015254263
1201	2	0.6092968835853547
1201	3	0.5009740628109824
1201	4	0.4752514008383102
1201	5	0.3091616312152894
1201	6	0.2999173192553357
1201	7	0.14353862655298144
1201	8	0.28251805794241136
1201	9	0.10251451843388537
1201	10	0.11270733887677409
1201	11	0.044658063198269815
1201	12	0.1285535829319926
1201	13	0.14871082936964797
1201	14	0.26520981110583364
1201	15	0.2261684681811431
1201	16	0.21556649535462213
1201	17	0.26854202775206204
1201	18	0.18143951259114743
1201	19	0.20603144535417783
1201	20	0.15730282327525913
1201	21	0.22576090967821472
1201	22	0.43693844930800907
1201	23	0.4394268491729605
1201	24	0.4377914393624849
1201	25	0.4236059696145631
1202	1	0.5373471352021575
1202	2	0.7286208209282812
1202	3	0.7161886271802942
1202	4	0.5084008335387438
1202	5	0.46661048312153497
1202	6	0.38667191706324644
1202	7	0.3322984890130698
1202	8	0.5106874521569083
1202	9	0.647898484027877
1202	10	0.611419216591031
1202	11	0.6372363757756005
1202	12	0.7175128852779551
1202	13	0.7728314887480536
1202	14	0.9636349720773083
1202	15	0.8077968650560052
1202	16	0.9224179038769122
1202	17	0.9727891509456228
1202	18	0.9041795151849215
1202	19	0.6346659656293951
1202	20	1.0428001436303944
1202	21	0.9335289004528065
1202	22	0.9628358411670774
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2210	18	0.020264399962329094
2210	19	0.12157182386373222
2210	20	0.032778038540975274
2210	21	0.0748287043916136
2210	22	-0.152801697069485
2210	23	0.06842001630484873
2210	24	-0.15258339317309577
2210	25	-0.005659297225399484
2211	1	-0.0455383636613611
2211	2	-0.3410455987108705
2211	3	-0.18421112237420445
2211	4	-0.051332438892210064
2211	5	-0.20407232267599795
2211	6	-0.1491354201199826
2211	7	-0.12665687979590246
2211	8	-0.061173472906752124
2211	9	-0.14789365863284587
2211	10	-0.10856810577055143
2211	11	-0.21881339016971424
2211	12	-0.033691568053271226
2211	13	-0.049123819469265584
2211	14	-0.18430343572938312
2211	15	-0.30735679693268025
2211	16	-0.06802881197774724
2211	17	0.04816827603320917
2211	18	0.03496271669256146
2211	19	-0.19036242699830552
2211	20	0.007207984501171438
2211	21	-0.09146593372026501
2211	22	-0.2728967663093158
2211	23	-0.08542123167025295
2211	24	0.17037990993026142
2211	25	0.042959520191487854
2212	1	-0.07753310847235184
2212	2	-0.9013030917672147
2212	3	-0.1994948703660384
2212	4	-0.2244749494775249
2212	5	-0.10442542319549375
2212	6	-5.348663082216376E-4
2212	7	-0.11622633807163396
2212	8	-0.12882702821299574
2212	9	-0.06671695570368996
2212	10	0.08468194570284737
2212	11	0.023638228336061218
2212	12	0.05204700870372392
2212	13	0.11628997764290006
2212	14	-0.08645215253604732
2212	15	-0.2322042325769189
2212	16	-0.0011792221542137327
2212	17	-1.2101679636213376
2212	18	-0.41877915436202073
2212	19	-0.2561363278136213
2212	20	-0.11571329551271958
2212	21	0.16038675851362427
2212	22	-0.0013371152919603446
2212	23	0.020286222938615595
2212	24	-0.03167734529796462
2212	25	-0.013658418323359456
2213	1	-0.2772576170230465
2213	2	-0.6009956769990801
2213	3	-0.28871307508009636
2213	4	-0.6595272969282107
2213	5	-0.5689355225109765
2213	6	-0.5487832270118389
2213	7	-0.4040809416179698
2213	8	-0.3404623307849306
2213	9	-0.5686398262485213
2213	10	-0.40724281307232957
2213	11	-0.5195743986270571
2213	12	-0.6233852126566433
2213	13	-0.5162047006719277
2213	14	-0.7278907938795953
2213	15	-0.6617358835287135
2213	16	-0.6640173546264805
2213	17	-1.4047127438870177
2213	18	-1.1964312010542884
2213	19	-0.9831444064509398
2213	20	-0.6640078431457113
2213	21	-0.914786544354449
2213	22	-0.6077280278777621
2213	23	-0.5759788846022218
2213	24	-0.4408038825416085
2213	25	-0.4757015922348131
2214	1	-0.7648693075084338
2214	2	-1.8981065306105123
2214	3	-0.9001370483001274
2214	4	-0.6372703122494414
2214	5	-0.5527581052327477
2214	6	-0.5732572839192684
2214	7	-0.33030084634309276
2214	8	-0.2640647512819072
2214	9	-0.5882162392780046
2214	10	-0.560646958817145
2214	11	-0.6700559230251528
2214	12	-0.6121981391404816
2214	13	-0.7202229831490712
2214	14	-0.5914957893733543
2214	15	-0.739674598605319
2214	16	-0.6752211656217142
2214	17	-0.48049361166093263
2214	18	-0.5879285616825882
2214	19	-0.5713779586589993
2214	20	-0.6790883117951267
2214	21	-0.6159581678899144
2214	22	-0.656206204780041
2214	23	-0.8296147800573479
2214	24	-0.6326308427579121
2214	25	-0.7646509935480594
2215	1	-0.9549219895007717
2215	2	-2.0233507834840765
2215	3	-1.189758695809708
2215	4	-1.0653977068854559
2215	5	-0.9853969941216363
2215	6	-0.682687360547618
2215	7	-0.7602337965346635
2215	8	-0.7067467436190711
2215	9	-0.9462382699293457
2215	10	-0.7768299090087183
2215	11	-0.8092752716841503
2215	12	-0.808658867109834
2215	13	-0.763905933340645
2215	14	-0.91588276255343
2215	15	-1.024923640750913
2215	16	-0.9513083303726759
2215	17	-0.9420549143429255
2215	18	-1.0829525080427373
2215	19	-0.8564162728352471
2215	20	-1.0934465493430139
2215	21	-0.8865520376217191
2215	22	-0.982872871446709
2215	23	-1.0447201440420244
2215	24	-0.760202681838372
2215	25	-0.9216050165365672
2216	1	-0.7165066107736457
2216	2	-1.8321304911461027
2216	3	-0.9778842881403911
2216	4	-0.6840802723152137
2216	5	-0.4286448799567282
2216	6	-0.42705338445955476
2216	7	-0.5043788355373593
2216	8	-0.4050043801285099
2216	9	-0.36891861184084007
2216	10	-0.2944289649247338
2216	11	-0.372524797819608
2216	12	-0.2954703965987875
2216	13	-0.30330731483805806
2216	14	-0.5127971753259004
2216	15	-0.5111451177502531
2216	16	-0.5136237965824861
2216	17	-0.5327863060466527
2216	18	-0.7109118832705619
2216	19	-0.585939856709274
2216	20	-0.8445264137448518
2216	21	-1.12347519866276
2216	22	-0.8026403133071724
2216	23	-0.8900352639662046
2216	24	-1.0128704481137945
2216	25	-1.0015149822725107
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	8689.0
3	0.0
4	116.0
5	1117.0
6	2312.0
7	1493.0
8	1200.0
9	757.0
10	748.0
11	696.0
12	689.0
13	538.0
14	536.0
15	502.0
16	499.0
17	559.0
18	715.0
19	1090.0
20	1510.0
21	1782.0
22	2085.0
23	2391.0
24	2884.0
25	3101.0
26	3536.0
27	3920.0
28	4457.0
29	5269.0
30	6661.0
31	8857.0
32	11779.0
33	16343.0
34	22362.0
35	31765.0
36	50300.0
37	96312.0
38	231124.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	51.188703339509885	12.419998408494095	15.077434340908145	21.31386391108787
2	6.967030929902185	23.564874103134983	38.663607284255846	30.804487682706988
3	24.10922007815485	31.774145346835788	25.900615478897056	18.21601909611231
4	14.952596398690432	13.528404268218747	29.651502061355643	41.867497271735175
5	18.086612265907682	30.968691808093503	27.966367916672972	22.978328009325843
6	40.300391576469075	26.083837865766302	11.0129699059573	22.60280065180733
7	23.86900346659804	33.09125175979049	22.04128127885678	20.998463494754688
8	25.816015317551617	34.36270206453956	26.196683719803836	13.624598898104983
9	28.723613565247664	24.133631991766713	12.952859137396752	34.18989530558888
10	12.432490716586807	25.043555901942945	29.801695322446093	32.722258059024156
11	28.776100540882098	28.01141168524786	14.674572109099646	28.537915664770395
12	27.54739112338719	23.204056150440806	23.35824283930531	25.890309886866696
13	34.78270738987821	19.213759027848713	27.490059435307828	18.51347414696524
14	36.62955228172126	17.836091374783404	22.341649074221202	23.19270726927413
15	39.416591322637636	13.517475401277885	28.87044483871334	18.195488437371143
16	29.565852265311875	17.966827504156495	35.410854355379605	17.05646587515203
17	25.00406662455031	30.427808902692295	29.66139203395537	14.906732438802027
18	27.906690826829887	13.983703238546305	44.51421805429984	13.595387880323967
19	23.23820584307747	38.89433207110351	16.714772628401306	21.15268945741771
20	15.993213452797747	29.983374094228598	32.62611761457027	21.397294838403383
21	17.11726196443535	37.37277058889177	19.887226649466303	25.62274079720658
22	16.332641638276904	34.16380877437563	14.859274420082738	34.64427516726472
23	23.052726749992434	24.51691735011954	16.4441031383349	35.986252761553125
24	14.78738837913548	30.780215893335754	18.53232631027432	35.90006941725444
25	28.545484844697445	28.94827540496832	16.858120007726306	25.648119742607932
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	482.0
1	482.0
2	586.0
3	690.0
4	690.0
5	690.0
6	1592.5
7	2495.0
8	2495.0
9	2495.0
10	2132.5
11	1770.0
12	1770.0
13	1770.0
14	1289.0
15	808.0
16	808.0
17	808.0
18	1226.5
19	1645.0
20	1645.0
21	1645.0
22	1995.0
23	2345.0
24	2345.0
25	2345.0
26	3771.5
27	5198.0
28	5198.0
29	5198.0
30	5747.5
31	6297.0
32	6297.0
33	6297.0
34	6098.5
35	5900.0
36	5900.0
37	5900.0
38	6988.0
39	8076.0
40	8076.0
41	8076.0
42	13293.0
43	18510.0
44	18510.0
45	18510.0
46	67955.0
47	117400.0
48	117400.0
49	117400.0
50	200884.0
51	284368.0
52	284368.0
53	284368.0
54	168092.5
55	51817.0
56	51817.0
57	51817.0
58	30387.0
59	8957.0
60	8957.0
61	8957.0
62	6894.0
63	4831.0
64	4831.0
65	4831.0
66	4015.5
67	3200.0
68	3200.0
69	3200.0
70	2597.0
71	1994.0
72	1994.0
73	1994.0
74	1545.0
75	1096.0
76	1096.0
77	1096.0
78	817.0
79	538.0
80	538.0
81	538.0
82	368.5
83	199.0
84	199.0
85	199.0
86	130.0
87	61.0
88	61.0
89	61.0
90	37.0
91	13.0
92	13.0
93	13.0
94	7.5
95	2.0
96	2.0
97	2.0
98	2.0
99	2.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.1687176325057595
2	0.008889830412298986
3	0.0
4	0.16758276053823196
5	0.051069238538738855
6	0.059959068951037836
7	0.04199026279851861
8	0.028371799188188253
9	0.02042769541549554
10	0.011916155659039066
11	0.021373422055101815
12	0.021562567383023073
13	0.00983555705190526
14	0.01097042901943279
15	0.0015131626233700402
16	5.67435983763765E-4
17	0.0
18	0.0
19	0.0
20	1.8914532792125503E-4
21	0.004350342542188865
22	0.007754958444771456
23	0.00113487196752753
24	0.001324017295448785
25	0.11878326593454815
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	528694.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	13.090180709446294
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.7702255552184	11.227477520077777
2	7.093213114280347	1.8570288295308817
3	2.1905298596962735	0.8602329513858679
4	1.1212738595806782	0.5871070978675755
5	0.6198794919589059	0.405716728391092
6	0.4522663892380829	0.3552149258361169
7	0.31933185949398185	0.29260782229418153
8	0.31066221624980134	0.32532996402455866
9	0.19362203245336454	0.22810926547303356
>10	1.4781741731327755	3.853457765739728
>50	0.12715476758131403	1.1500035937612305
>100	0.17628274596500354	5.2101972029188905
>500	0.041903275680205763	3.9294941875640728
>1k	0.07947172973832127	21.85763409458023
>5k	0.013004464866270753	11.858844624678925
>10k+	0.013004464866270753	36.00154342587584
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTACGGAAGCAGTGGTATCAACGCA	44853	8.48373539325205	No Hit
CTTCCGTACTCTGCGTTGATACCAC	28114	5.3176317491781635	No Hit
GAGTACGGAAGCAGTGGTATCAACG	26945	5.096520860838217	No Hit
TCCGTACTCTGCGTTGATACCACTG	25439	4.811667996988806	No Hit
GCTTCCGTACTCTGCGTTGATACCA	18180	3.438662061608416	No Hit
CCCATGTACTCTGCGTTGATACCAC	13026	2.463807041502268	No Hit
GCGTTGATACCACTGCTTCCGTACT	11779	2.227942817584463	No Hit
GTATCAACGCAGAGTACGGAAGCAG	11143	2.1076463890265447	No Hit
GTACATGGGAAGCAGTGGTATCAAC	10859	2.053929115896908	No Hit
TCCATGTACTCTGCGTTGATACCAC	9071	1.715737269573704	No Hit
TATCAACGCAGAGTACGGAAGCAGT	8652	1.6364853771746986	No Hit
GTACATGGAAGCAGTGGTATCAACG	8309	1.571608529697708	No Hit
ACTCTGCGTTGATACCACTGCTTCC	7776	1.4707940699156792	No Hit
CAGTGGTATCAACGCAGAGTACGGA	6506	1.2305795034556852	No Hit
ACGCAGAGTACGGAAGCAGTGGTAT	5939	1.1233341025243335	No Hit
CATGTACTCTGCGTTGATACCACTG	5778	1.0928817047290116	No Hit
GGTATCAACGCAGAGTACGGAAGCA	5485	1.0374621236480837	No Hit
ATACCACTGCTTCCGTACTCTGCGT	5181	0.9799619439600223	No Hit
AAGCAGTGGTATCAACGCAGAGTAC	4479	0.8471819237593011	No Hit
GAGTACATGGGAAGCAGTGGTATCA	4199	0.7942212319413497	No Hit
GTGGTATCAACGCAGAGTACGGAAG	4077	0.7711455019349567	No Hit
GAAGCAGTGGTATCAACGCAGAGTA	3850	0.7282095124968319	No Hit
GTACGGGAAGCAGTGGTATCAACGC	3629	0.6864083950262344	No Hit
GAGTACATGGAAGCAGTGGTATCAA	3342	0.6321236859128342	No Hit
GTATCAACGCAGAGTACATGGGAAG	3308	0.6256927447635117	No Hit
GCGTTGATACCACTGCTTCCCATGT	3184	0.602238724101276	No Hit
CCCGTACTCTGCGTTGATACCACTG	2925	0.5532500841696709	No Hit
ATCAACGCAGAGTACGGAAGCAGTG	2835	0.536227004656758	No Hit
GATACCACTGCTTCCGTACTCTGCG	2834	0.5360378593288367	No Hit
CCACTGCTTCCGTACTCTGCGTTGA	2796	0.528850336867829	No Hit
GTTGATACCACTGCTTCCGTACTCT	2730	0.5163667452250262	No Hit
GTACTCTGCGTTGATACCACTGCTT	2699	0.5105032400594672	No Hit
CGGAAGCAGTGGTATCAACGCAGAG	2636	0.4985870844004282	No Hit
TTCCCGTACTCTGCGTTGATACCAC	2610	0.4936693058744756	No Hit
TATCAACGCAGAGTACATGGGAAGC	2487	0.47040443054016123	No Hit
GTATCAACGCAGAGTACTTTTTTTT	2362	0.4467612645500043	No Hit
GAGTACGGGAAGCAGTGGTATCAAC	2349	0.444302375287028	No Hit
GCTTCCCATGTACTCTGCGTTGATA	2309	0.43673656217017787	No Hit
CATGGAAGCAGTGGTATCAACGCAG	2296	0.4342776729072015	No Hit
CTGCTTCCGTACTCTGCGTTGATAC	2161	0.4087430536378321	No Hit
CAGTGGTATCAACGCAGAGTACATG	2117	0.4004206592092969	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	2114	0.3998532232255331	No Hit
ACGCAGAGTACATGGGAAGCAGTGG	2082	0.393800572732053	No Hit
GCTTCCATGTACTCTGCGTTGATAC	2040	0.3858564689593602	No Hit
AAAAAGTACTCTGCGTTGATACCAC	1980	0.3745077492840849	No Hit
GTATCAACGCAGAGTACGGGAAGCA	1940	0.3669419361672347	No Hit
GCAGTGGTATCAACGCAGAGTACGG	1840	0.34802740337510923	No Hit
GCGTTGATACCACTGCTTCCATGTA	1812	0.3427313341933141	No Hit
CTTCCCGTACTCTGCGTTGATACCA	1811	0.34254218886539284	No Hit
GCAGAGTACGGAAGCAGTGGTATCA	1808	0.34197475288162904	No Hit
TATCAACGCAGAGTACTTTTTTTTT	1737	0.3285454345992199	No Hit
GTATCAACGCAGAGTACATGGAAGC	1630	0.3083068845116457	No Hit
CACTGCTTCCGTACTCTGCGTTGAT	1573	0.29752560082013413	No Hit
GCGTTGATACCACTGCTTCCCGTAC	1498	0.28333970122604	No Hit
TGATACCACTGCTTCCGTACTCTGC	1476	0.2791785040117724	No Hit
CCATGTACTCTGCGTTGATACCACT	1434	0.2712344002390797	No Hit
AAAGTACTCTGCGTTGATACCACTG	1427	0.2699103829436309	No Hit
GGTATCAACGCAGAGTACATGGGAA	1396	0.264046877778072	No Hit
TACCACTGCTTCCGTACTCTGCGTT	1346	0.25458961138200925	No Hit
CTGCGTTGATACCACTGCTTCCGTA	1345	0.254400466054088	No Hit
GCCCATGTACTCTGCGTTGATACCA	1308	0.24740208892100155	No Hit
TATCAACGCAGAGTACATGGAAGCA	1283	0.2426734557229702	No Hit
TATCAACGCAGAGTACGGGAAGCAG	1274	0.2409711477716789	No Hit
GGGAAGCAGTGGTATCAACGCAGAG	1239	0.23435106129443498	No Hit
CCCCATGTACTCTGCGTTGATACCA	1234	0.2334053346548287	No Hit
TACGGAAGCAGTGGTATCAACGCAG	1150	0.21751712710944326	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	1126	0.21297763923933316	No Hit
ATACCACTGCTTCCCATGTACTCTG	1105	0.20900558735298677	No Hit
GCTTCCCGTACTCTGCGTTGATACC	1098	0.20768157005753798	No Hit
TCAACGCAGAGTACGGAAGCAGTGG	1084	0.20503353546664044	No Hit
ACGCAGAGTACATGGAAGCAGTGGT	1082	0.20465524481079791	No Hit
CTGCTTCCCATGTACTCTGCGTTGA	1047	0.198035158333554	No Hit
CATGGGAAGCAGTGGTATCAACGCA	1027	0.1942522517751289	No Hit
TACCACTGCTTCCCATGTACTCTGC	965	0.1825252414440111	No Hit
GGTATCAACGCAGAGTACTTTTTTT	945	0.178742334885586	No Hit
ACGGAAGCAGTGGTATCAACGCAGA	938	0.1774183175901372	No Hit
GCAGTGGTATCAACGCAGAGTACAT	938	0.1774183175901372	No Hit
ATACCACTGCTTCCATGTACTCTGC	928	0.17552686431092465	No Hit
CTCTGCGTTGATACCACTGCTTCCG	889	0.1681501965219957	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	861	0.16285412734020058	No Hit
GTGGTATCAACGCAGAGTACATGGG	844	0.15963865676553923	No Hit
GGTATCAACGCAGAGTACGGGAAGC	817	0.15453173291166536	No Hit
AGCAGTGGTATCAACGCAGAGTACG	814	0.1539642969279016	No Hit
GCAGAGTACATGGGAAGCAGTGGTA	810	0.15320771561621657	No Hit
TCCCATGTACTCTGCGTTGATACCA	790	0.14942480905779146	No Hit
ACATGGGAAGCAGTGGTATCAACGC	700	0.1324017295448785	No Hit
GGTATCAACGCAGAGTACATGGAAG	687	0.1299428402819022	No Hit
CCACTGCTTCCCATGTACTCTGCGT	678	0.1282405323306109	No Hit
CTTCCCATGTACTCTGCGTTGATAC	673	0.12729480569100463	No Hit
GTTGATACCACTGCTTCCCATGTAC	671	0.12691651503516213	No Hit
AACGCAGAGTACGGAAGCAGTGGTA	653	0.12351189913257953	No Hit
CAACGCAGAGTACGGAAGCAGTGGT	642	0.12143130052544573	No Hit
GATACCACTGCTTCCCATGTACTCT	621	0.11745924863909936	No Hit
TTGATACCACTGCTTCCGTACTCTG	596	0.112730615441068	No Hit
CCACTGCTTCCATGTACTCTGCGTT	571	0.10800198224303661	No Hit
GGAAGCAGTGGTATCAACGCAGAGT	567	0.1072454009313516	No Hit
ATCAACGCAGAGTACATGGGAAGCA	561	0.10611052896382406	No Hit
CAGTGGTATCAACGCAGAGTACGGG	548	0.10365163970084774	No Hit
AGTGGTATCAACGCAGAGTACGGAA	530	0.10024702379826517	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	1.8914532792125503E-4
8	0.0	0.0	0.0	0.0	1.8914532792125503E-4
9	0.0	0.0	0.0	1.8914532792125503E-4	1.8914532792125503E-4
10	0.0	0.0	0.0	7.565813116850201E-4	1.8914532792125503E-4
11	0.0	0.0	0.0	9.457266396062751E-4	1.8914532792125503E-4
12	0.0	0.0	0.0	0.0024588892629763153	1.8914532792125503E-4
13	0.0	0.0	0.0	0.0032154705746613352	1.8914532792125503E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTCCGT	25	0.005928539	19.054207	1
GTTATCA	55	2.737761E-8	19.054205	1
GTCCGTA	25	0.005980753	19.025309	2
TTATCAA	60	7.5409844E-8	17.439865	2
TACATGA	40	2.7289317E-4	16.647144	2
TACGAAA	40	2.7289317E-4	16.647144	2
CGAAAGC	40	2.7289317E-4	16.647144	4
ACGAAAG	35	0.0021484573	16.307405	3
AGGCAAA	35	0.0021744422	16.27809	11
GGACGGA	70	4.2795E-7	14.971161	1
TACGGAG	45	6.684062E-4	14.7974615	2
GACGGAA	65	3.315814E-6	14.634852	2
GCCCATG	285	0.0	14.03994	1
ACAAAGA	50	0.001502556	13.293775	8
GTACGAA	65	5.2956893E-5	13.191373	1
AAAAAGT	355	0.0	12.613346	1
CCCCATG	320	0.0	12.504321	1
GAGTATG	55	0.0029991348	12.125403	1
AGTATGG	55	0.0030334347	12.107015	2
AAAAGTA	380	0.0	11.765651	2
>>END_MODULE