FastQCFastQC Report
Thu 2 Feb 2017
SRR4050225_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4050225_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7192658
Sequences flagged as poor quality0
Sequence length25
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACAAAGGGCAGGGACTTAATCAA138240.1921959865184748No Hit
GTATCTGATCGTCTTCGAACCTCCG107090.14888793544750772No Hit
GAATAGGACCGCGGTTCTATTTTGT100820.1401707129686967No Hit
GTCCTATTCCATTATTCCTAGCTGC96190.13373359333920784No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG95610.13292721550225245No Hit
CTATTGGAGCTGGAATTACCGCGGC88600.12318116612801554No Hit
GATTAAGAGGGACGGCCGGGGGCAT88030.12238869135721454No Hit
GTCTTGCGCCGGTCCAAGAATTTCA85540.11892682788476805No Hit
GAACTACGACGGTATCTGATCGTCT84870.11799532245242303No Hit
GTGTAGCGCGCGTGCAGCCCCGGAC82890.1152425153538511No Hit
ATACAGGACTCTTTCGAGGCCCTGT82060.1140885608630356No Hit
CTCTTAATCATGGCCTCAGTTCCGA78960.1097786103551705No Hit
GGGTAGGCACACGCTGAGCCAGTCA77790.10815195161510528No Hit
CTTTAATATACGCTATTGGAGCTGG73890.1027297558148879No Hit
ATCAGATACCGTCGTAGTTCCGACC72990.10147847986099158No Hit
GACTTAATCAACGCAAGCTTATGAC72060.10018549470863206No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA38100.016.1764971
GTATCAA133250.013.5136221
ACGCTTA5000.013.5014117
CGCTTAC5400.012.85354418
CGAACGA7600.012.51118516
CGGTAGC5400.012.50366810
TCGACGC5600.012.3950114
CTCGACG5450.012.38748113
GACGCTT5800.012.13153616
CGGTTCT18950.011.94140512
GGTAGCG5750.011.90796411
TATATCG752.0570302E-411.4111595
CCGGTAG6100.011.3806199
CGTCGTA14350.011.332273510
GGTTCTA20650.011.18941513
CGACGCT6300.011.168715515
ATCGCCA11950.011.06009816
ACTCTAA13000.010.97288310
CGCCAGT12100.010.92252918
CGTCTTA2900.010.82001815
Produced by FastQC (version 0.11.5)