##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR4050225_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 7192658 Sequences flagged as poor quality 0 Sequence length 25 %GC 52 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.537426497965008 31.0 31.0 34.0 27.0 34.0 2 30.627800737919138 31.0 31.0 34.0 27.0 34.0 3 30.741332202921367 31.0 31.0 34.0 27.0 34.0 4 34.26877713357148 37.0 35.0 37.0 30.0 37.0 5 34.27741288408263 37.0 35.0 37.0 30.0 37.0 6 34.31457661409732 37.0 35.0 37.0 31.0 37.0 7 34.2610315963862 37.0 35.0 37.0 30.0 37.0 8 34.22757567508423 37.0 35.0 37.0 30.0 37.0 9 35.80219162373631 39.0 37.0 39.0 30.0 39.0 10 35.687084107154824 39.0 37.0 39.0 30.0 39.0 11 35.5791469579118 39.0 37.0 39.0 30.0 39.0 12 35.49726679622471 39.0 37.0 39.0 30.0 39.0 13 35.49874483118758 39.0 37.0 39.0 30.0 39.0 14 36.67476891018592 40.0 37.0 41.0 30.0 41.0 15 36.617321440835916 40.0 37.0 41.0 30.0 41.0 16 36.61581059463692 40.0 37.0 41.0 30.0 41.0 17 36.57488469492085 40.0 37.0 41.0 30.0 41.0 18 36.53203808661554 40.0 37.0 41.0 30.0 41.0 19 36.54108953880471 40.0 37.0 41.0 30.0 41.0 20 36.4922957549212 40.0 37.0 41.0 30.0 41.0 21 36.43563033304239 40.0 37.0 41.0 30.0 41.0 22 36.34501960193297 40.0 37.0 41.0 30.0 41.0 23 36.2590630612494 40.0 37.0 41.0 30.0 41.0 24 36.18193788721777 40.0 37.0 41.0 29.0 41.0 25 35.93775013353895 40.0 37.0 41.0 28.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.5458105671977513 1101 2 0.5473697517435561 1101 3 0.5266131762580137 1101 4 0.5492747135814326 1101 5 0.5683906383975881 1101 6 0.5519754342090053 1101 7 0.5585618299107438 1101 8 0.5536280678943015 1101 9 0.5941988290392288 1101 10 0.6218192829173859 1101 11 0.4819449821523065 1101 12 0.4382852689536776 1101 13 0.42401640453329037 1101 14 0.5782077687905627 1101 15 0.6004960943884114 1101 16 0.6190905127005308 1101 17 0.628668646501211 1101 18 0.6388286237582506 1101 19 0.6929500885267998 1101 20 0.6653038471693336 1101 21 0.6790181097541961 1101 22 0.6743773014672811 1101 23 0.7175727228323012 1101 24 0.76051838317521 1101 25 0.7386971784517868 1102 1 0.5004023011955887 1102 2 0.5173436687814892 1102 3 0.5511783553463374 1102 4 0.48050898199996794 1102 5 0.4644523942937866 1102 6 0.5023682517981669 1102 7 0.4877590158454552 1102 8 0.5703339061988189 1102 9 0.6368433708097214 1102 10 0.6852639643843261 1102 11 0.6294744945991297 1102 12 0.6092683669425512 1102 13 0.6017710044477127 1102 14 0.7648964911344436 1102 15 0.6762546712466246 1102 16 0.7278295635198262 1102 17 0.7131379573320942 1102 18 0.7838151260613557 1102 19 0.8107516314803078 1102 20 0.7582592449895671 1102 21 0.7796712850165974 1102 22 0.8363041999545331 1102 23 0.8189442021043689 1102 24 0.7936888946403826 1102 25 0.8626470953890362 1103 1 0.5680764754362819 1103 2 0.5170223278008557 1103 3 0.49447334995619485 1103 4 0.4962830436668071 1103 5 0.5289539624895738 1103 6 0.502930507637295 1103 7 0.5196428841601701 1103 8 0.5675546156980715 1103 9 0.6084836661144521 1103 10 0.6278971474298629 1103 11 0.6587417876353427 1103 12 0.593758030254719 1103 13 0.5616129239511594 1103 14 0.7092594719446339 1103 15 0.7616390865271327 1103 16 0.7106786248174828 1103 17 0.6828948273473472 1103 18 0.713946772718046 1103 19 0.7231784442771456 1103 20 0.688659523981876 1103 21 0.679447795817893 1103 22 0.7380326572838172 1103 23 0.8030590887751003 1103 24 0.7812731006766569 1103 25 0.8336542962532789 1104 1 0.5363031143922647 1104 2 0.5577834395548642 1104 3 0.5268922441465627 1104 4 0.5638957621297962 1104 5 0.6148084885416978 1104 6 0.5564103231877908 1104 7 0.5657550027230087 1104 8 0.5165517355976093 1104 9 0.5977099186638313 1104 10 0.5905869744120125 1104 11 0.530896730217286 1104 12 0.5778073886257786 1104 13 0.5540373923856663 1104 14 0.6442587550968355 1104 15 0.511872877015378 1104 16 0.6574272640887457 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2215 20 -0.7817400558429455 2215 21 -0.6868330986922331 2215 22 -0.7746926583101867 2215 23 -0.7160708430763592 2215 24 -0.7773009101182851 2215 25 -0.7209980690235298 2216 1 -0.5345804556785332 2216 2 -0.6971576344138128 2216 3 -0.661457324641102 2216 4 -0.6124329806512279 2216 5 -0.6349536830723252 2216 6 -0.5848900763424538 2216 7 -0.6561796801184343 2216 8 -0.6517406676633826 2216 9 -0.6441433737339537 2216 10 -0.7673826081235546 2216 11 -0.5880379990814362 2216 12 -0.6176812392367808 2216 13 -0.6052687797350202 2216 14 -0.6903454749488702 2216 15 -0.6828771430955172 2216 16 -0.9014953056648238 2216 17 -0.9464270520254772 2216 18 -0.8469232768543122 2216 19 -0.816463328467826 2216 20 -0.7089055656614462 2216 21 -0.8099697285293459 2216 22 -0.8462017257927243 2216 23 -0.9055845972232675 2216 24 -1.050753443821769 2216 25 -0.9996758165384279 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 194549.0 3 15.0 4 1657.0 5 9413.0 6 12497.0 7 12353.0 8 10576.0 9 9928.0 10 10319.0 11 12010.0 12 19813.0 13 17124.0 14 8195.0 15 8405.0 16 8847.0 17 9522.0 18 10323.0 19 12377.0 20 15403.0 21 19484.0 22 23881.0 23 28875.0 24 34048.0 25 38648.0 26 44961.0 27 50707.0 28 59247.0 29 72117.0 30 91442.0 31 116824.0 32 152343.0 33 202405.0 34 280055.0 35 395296.0 36 592206.0 37 1104977.0 38 3501816.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.026276200494124 16.758416981616755 12.104149683495177 26.11115713439394 2 17.779706916645186 19.745256191359065 36.51903044896901 25.95600644302674 3 19.35178142838779 23.35599640618269 27.27519874698281 30.01702341844671 4 13.659244507767717 15.617221763766173 35.541960058417395 35.181573670048714 5 16.7768622208281 34.164196854370545 31.770682002485458 17.288258922315894 6 34.12293269454173 35.10310197728409 15.550956364853588 15.22300896332059 7 30.925443527532 29.624178110451826 20.02621366165235 19.424164700363825 8 27.919672260910318 31.168364146630108 18.939566671364048 21.972396921095527 9 27.828857772361932 14.838626743070323 17.97392390584092 39.35859157872682 10 17.36280688007942 24.75500234841588 28.989301908338323 28.892888863166377 11 37.49280527845795 21.400738604408133 21.25724319971931 19.849212917414608 12 26.29497349313957 22.958118040899496 26.67888911903176 24.06801934692918 13 30.0488224177271 19.316077502334338 23.914503809956717 26.72059626998184 14 24.871264537363338 19.57709692032725 23.57440038558711 31.977238156722297 15 25.917280038716157 25.604138755107 21.91422182046837 26.56435938570847 16 26.59878151019397 25.458389258926985 22.056023098961358 25.886806131917687 17 26.231737255508946 24.407890888297317 23.217822876813816 26.142548979379914 18 27.721735450043518 23.055487362973732 23.908961456992905 25.313815729989848 19 28.291238981897983 23.690675795584642 23.20837053852592 24.80971468399146 20 27.12109794454516 23.49598919254227 23.18910419701552 26.193808665897052 21 27.470370140754163 23.614237558635907 23.188743697326696 25.72664860328323 22 26.72982310394235 23.397742804177163 23.826372652556373 26.046061439324113 23 25.4565955741434 22.822040510648208 24.226250313445934 27.495113601762462 24 25.87553726810513 24.39425658243956 23.51860385933186 26.211602290123448 25 25.88171417056624 23.674797931173455 23.7255670241587 26.7179208741016 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 2718.0 1 2718.0 2 3088.5 3 3459.0 4 3459.0 5 3459.0 6 6115.0 7 8771.0 8 8771.0 9 8771.0 10 12428.0 11 16085.0 12 16085.0 13 16085.0 14 21973.5 15 27862.0 16 27862.0 17 27862.0 18 44311.0 19 60760.0 20 60760.0 21 60760.0 22 86836.0 23 112912.0 24 112912.0 25 112912.0 26 154793.5 27 196675.0 28 196675.0 29 196675.0 30 235184.5 31 273694.0 32 273694.0 33 273694.0 34 348367.0 35 423040.0 36 423040.0 37 423040.0 38 475764.5 39 528489.0 40 528489.0 41 528489.0 42 603069.0 43 677649.0 44 677649.0 45 677649.0 46 733060.0 47 788471.0 48 788471.0 49 788471.0 50 820772.5 51 853074.0 52 853074.0 53 853074.0 54 809587.5 55 766101.0 56 766101.0 57 766101.0 58 734201.5 59 702302.0 60 702302.0 61 702302.0 62 641250.5 63 580199.0 64 580199.0 65 580199.0 66 507060.0 67 433921.0 68 433921.0 69 433921.0 70 354497.5 71 275074.0 72 275074.0 73 275074.0 74 233305.0 75 191536.0 76 191536.0 77 191536.0 78 165042.5 79 138549.0 80 138549.0 81 138549.0 82 108009.5 83 77470.0 84 77470.0 85 77470.0 86 56868.0 87 36266.0 88 36266.0 89 36266.0 90 26155.0 91 16044.0 92 16044.0 93 16044.0 94 8669.0 95 1294.0 96 1294.0 97 1294.0 98 768.5 99 243.0 100 243.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.14324329058881988 2 0.16073334781105955 3 0.17446957717161027 4 0.19428144644163534 5 0.18491077985356733 6 0.06840308547966552 7 0.05616838726379038 8 0.05918535261929596 9 0.06527489559492472 10 0.0672074217903868 11 0.6988932325157126 12 0.6581433456171557 13 0.655807630503216 14 0.6560995948924584 15 0.6579069934925309 16 0.6638436027404612 17 0.6548344158724076 18 0.6654007461497544 19 0.6617581428173006 20 0.6668188588975036 21 0.6545424514831653 22 0.6580599272202293 23 0.6435729322873408 24 0.6536804613815922 25 0.6475770153398089 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 7192658.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 40.670591325803606 #Duplication Level Percentage of deduplicated Percentage of total 1 82.15219935997912 33.411785266856555 2 11.16734691708776 9.08365205316698 3 2.739602745236249 3.342637909396594 4 1.0724617607113056 1.7447061592976514 5 0.5602287373937351 1.1392417013755776 6 0.3630464889964478 0.885918923174543 7 0.2662212330231288 0.757916247937464 8 0.19871847513474572 0.6465598312873686 9 0.14476736658008726 0.5298996963142374 >10 1.0230275980603474 8.202059682213228 >50 0.13252905553262848 3.76840277788058 >100 0.13932234049685155 12.078951212894351 >500 0.02185970129470103 6.217450530534994 >1k 0.017295557202870265 14.172276958468968 >5k 0.001269713524804578 3.5354279152990276 >10k+ 1.0294974525442525E-4 0.48311313390183624 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTACAAAGGGCAGGGACTTAATCAA 13824 0.1921959865184748 No Hit GTATCTGATCGTCTTCGAACCTCCG 10709 0.14888793544750772 No Hit GAATAGGACCGCGGTTCTATTTTGT 10082 0.1401707129686967 No Hit GTCCTATTCCATTATTCCTAGCTGC 9619 0.13373359333920784 No Hit GTTCAAAGCAGGCCCGAGCCGCCTG 9561 0.13292721550225245 No Hit CTATTGGAGCTGGAATTACCGCGGC 8860 0.12318116612801554 No Hit GATTAAGAGGGACGGCCGGGGGCAT 8803 0.12238869135721454 No Hit GTCTTGCGCCGGTCCAAGAATTTCA 8554 0.11892682788476805 No Hit GAACTACGACGGTATCTGATCGTCT 8487 0.11799532245242303 No Hit GTGTAGCGCGCGTGCAGCCCCGGAC 8289 0.1152425153538511 No Hit ATACAGGACTCTTTCGAGGCCCTGT 8206 0.1140885608630356 No Hit CTCTTAATCATGGCCTCAGTTCCGA 7896 0.1097786103551705 No Hit GGGTAGGCACACGCTGAGCCAGTCA 7779 0.10815195161510528 No Hit CTTTAATATACGCTATTGGAGCTGG 7389 0.1027297558148879 No Hit ATCAGATACCGTCGTAGTTCCGACC 7299 0.10147847986099158 No Hit GACTTAATCAACGCAAGCTTATGAC 7206 0.10018549470863206 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 1.390306615440356E-5 2 0.0 0.0 0.0 0.0 1.390306615440356E-5 3 0.0 0.0 0.0 1.390306615440356E-5 1.390306615440356E-5 4 0.0 0.0 0.0 1.390306615440356E-5 1.390306615440356E-5 5 0.0 2.780613230880712E-5 0.0 1.390306615440356E-5 1.390306615440356E-5 6 1.390306615440356E-5 2.780613230880712E-5 0.0 1.390306615440356E-5 1.390306615440356E-5 7 1.390306615440356E-5 2.780613230880712E-5 0.0 1.390306615440356E-5 1.390306615440356E-5 8 1.390306615440356E-5 2.780613230880712E-5 0.0 1.390306615440356E-5 1.390306615440356E-5 9 1.390306615440356E-5 2.780613230880712E-5 0.0 8.341839692642136E-5 1.390306615440356E-5 10 1.390306615440356E-5 2.780613230880712E-5 0.0 8.341839692642136E-5 1.390306615440356E-5 11 1.390306615440356E-5 2.780613230880712E-5 0.0 8.341839692642136E-5 1.390306615440356E-5 12 2.780613230880712E-5 2.780613230880712E-5 0.0 9.732146308082492E-5 4.170919846321068E-5 13 2.780613230880712E-5 2.780613230880712E-5 0.0 1.1122452923522848E-4 8.341839692642136E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3810 0.0 16.176497 1 GTATCAA 13325 0.0 13.513622 1 ACGCTTA 500 0.0 13.50141 17 CGCTTAC 540 0.0 12.853544 18 CGAACGA 760 0.0 12.511185 16 CGGTAGC 540 0.0 12.503668 10 TCGACGC 560 0.0 12.39501 14 CTCGACG 545 0.0 12.387481 13 GACGCTT 580 0.0 12.131536 16 CGGTTCT 1895 0.0 11.941405 12 GGTAGCG 575 0.0 11.907964 11 TATATCG 75 2.0570302E-4 11.411159 5 CCGGTAG 610 0.0 11.380619 9 CGTCGTA 1435 0.0 11.3322735 10 GGTTCTA 2065 0.0 11.189415 13 CGACGCT 630 0.0 11.1687155 15 ATCGCCA 1195 0.0 11.060098 16 ACTCTAA 1300 0.0 10.972883 10 CGCCAGT 1210 0.0 10.922529 18 CGTCTTA 290 0.0 10.820018 15 >>END_MODULE