FastQCFastQC Report
Thu 2 Feb 2017
SRR4050225_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4050225_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7192658
Sequences flagged as poor quality0
Sequence length25
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACAAAGGGCAGGGACTTAATCAA132880.1847439430597145No Hit
GAATAGGACCGCGGTTCTATTTTGT104120.14475872479964988No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG96800.13458168037462645No Hit
GATTAAGAGGGACGGCCGGGGGCAT95880.13330259828842134No Hit
GTATCTGATCGTCTTCGAACCTCCG95750.1331218584284141No Hit
CTATTGGAGCTGGAATTACCGCGGC91200.12679596332816045No Hit
GTATCAACGCAGAGTACTTTTTTTT89240.12407096236189738No Hit
ATACAGGACTCTTTCGAGGCCCTGT87090.12108180313870061No Hit
GTCCTATTCCATTATTCCTAGCTGC86130.11974710878787785No Hit
GAACTACGACGGTATCTGATCGTCT83120.11556228587540239No Hit
ATCAGATACCGTCGTAGTTCCGACC82600.1148393264353734No Hit
CTCTTAATCATGGCCTCAGTTCCGA78460.10908345704745033No Hit
GTCTTGCGCCGGTCCAAGAATTTCA78370.1089583294520607No Hit
GTGTAGCGCGCGTGCAGCCCCGGAC78020.10847172213665657No Hit
GGGTAGGCACACGCTGAGCCAGTCA77830.10820756387972291No Hit
TCGTAGTTCCGACCATAAACGATGC76470.10631674688272404No Hit
CTTTAATATACGCTATTGGAGCTGG75670.10520450159037173No Hit
CTTATGACCCGCACTTACTGGGAAT74060.10296610793951277No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA43050.015.9005861
GTATCAA137650.013.0996931
CGCTTAC5100.013.02794718
CTCGACG5350.012.06458313
CGGTAGC5450.012.02055510
ACGCTTA5450.011.84296817
ACCGTCC5400.011.7805368
TAGGTCG658.023219E-411.6934225
CGAACGA6700.011.61689916
GCTCGTA8950.011.4573659
GGTAGCG5650.011.09091611
CCGTCCA5450.010.97559459
AACCGCG9500.010.8952757
TATAACG803.706455E-410.70848752
TAGTACG6200.010.5933422
CTCGTAG9950.010.59184910
TCGACGC6100.010.58108514
CGTCGTA14600.010.46995810
GCATCGC14300.010.42112814
AAGCTCG10500.010.4002527
Produced by FastQC (version 0.11.5)