##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR4050225_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 7192658 Sequences flagged as poor quality 0 Sequence length 25 %GC 52 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.127345968625228 31.0 31.0 34.0 28.0 34.0 2 31.271482253153145 31.0 31.0 34.0 28.0 34.0 3 31.443530333292642 33.0 31.0 34.0 28.0 34.0 4 34.99931541302256 37.0 35.0 37.0 32.0 37.0 5 34.895246235814355 37.0 35.0 37.0 32.0 37.0 6 34.89054296756498 37.0 35.0 37.0 32.0 37.0 7 34.8394233397445 37.0 35.0 37.0 32.0 37.0 8 34.81952846916953 37.0 35.0 37.0 32.0 37.0 9 36.48629741605954 39.0 37.0 39.0 32.0 39.0 10 36.364450805251685 39.0 37.0 39.0 32.0 39.0 11 36.37478022728176 39.0 37.0 39.0 32.0 39.0 12 36.25885896423826 39.0 37.0 39.0 32.0 39.0 13 36.28075545924747 39.0 37.0 39.0 32.0 39.0 14 37.48514179320079 40.0 38.0 41.0 32.0 41.0 15 37.442217466755686 40.0 38.0 41.0 32.0 41.0 16 37.40984139660193 40.0 38.0 41.0 32.0 41.0 17 37.37346777783679 40.0 38.0 41.0 32.0 41.0 18 37.32612533502914 40.0 38.0 41.0 32.0 41.0 19 37.33116408426481 40.0 38.0 41.0 32.0 41.0 20 37.27959608256086 40.0 38.0 41.0 32.0 41.0 21 37.22877745612262 40.0 37.0 41.0 32.0 41.0 22 37.17638583677967 40.0 37.0 41.0 32.0 41.0 23 37.09135412805669 40.0 37.0 41.0 31.0 41.0 24 37.00872167145998 40.0 37.0 41.0 31.0 41.0 25 36.68894322516099 40.0 37.0 41.0 31.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.41071265602552387 1101 2 0.3813807725814584 1101 3 0.3807133786205519 1101 4 0.2878670588446184 1101 5 0.3782139382523084 1101 6 0.4004728925363352 1101 7 0.4770718576436792 1101 8 0.453943089553313 1101 9 0.49393555681903933 1101 10 0.503595357198428 1101 11 0.515635987687439 1101 12 0.48881702057749976 1101 13 0.4421681273374105 1101 14 0.519109752078478 1101 15 0.5845886731325294 1101 16 0.5823649330467546 1101 17 0.570878531127903 1101 18 0.6386346998749559 1101 19 0.6257511961222093 1101 20 0.6572899581325089 1101 21 0.6479772214907271 1101 22 0.7069053839171389 1101 23 0.6520686292587641 1101 24 0.6464698675708505 1101 25 0.76490753640973 1102 1 0.4041631267144403 1102 2 0.3686131783807518 1102 3 0.3703030045864715 1102 4 0.3104311807706708 1102 5 0.3213244494657772 1102 6 0.33461860473649097 1102 7 0.3588910262610625 1102 8 0.4501064714657659 1102 9 0.4555622967319479 1102 10 0.47074860167978727 1102 11 0.45624816644854604 1102 12 0.45079536133613374 1102 13 0.530388161821044 1102 14 0.5122260839770902 1102 15 0.5858149499076859 1102 16 0.5028186633193528 1102 17 0.5631395369423018 1102 18 0.5788001017224715 1102 19 0.6200590380009388 1102 20 0.6379578896183205 1102 21 0.5658879416196001 1102 22 0.5669852374246531 1102 23 0.6066370805160304 1102 24 0.6299532801913585 1102 25 0.8254154462488899 1103 1 0.35950218302522075 1103 2 0.3473044817951134 1103 3 0.4043730548300779 1103 4 0.3253269619207373 1103 5 0.42736833622852544 1103 6 0.38694388523394707 1103 7 0.46184382362472576 1103 8 0.449421531711522 1103 9 0.4815717411214919 1103 10 0.5062716384247565 1103 11 0.49779448360236955 1103 12 0.5099323254317838 1103 13 0.48153765330756926 1103 14 0.5845608838375966 1103 15 0.6364995829521405 1103 16 0.6386079468793611 1103 17 0.5577747056497202 1103 18 0.6356758649470606 1103 19 0.6125008909369996 1103 20 0.5960399765171545 1103 21 0.6097121810077226 1103 22 0.6781427260849142 1103 23 0.6409598141862887 1103 24 0.6327915952868182 1103 25 0.7557890528492877 1104 1 0.2390786118210535 1104 2 0.29782928350252647 1104 3 0.27479976767824255 1104 4 0.22858979890330744 1104 5 0.24285447832313878 1104 6 0.2723789516443773 1104 7 0.31450980844984855 1104 8 0.28777341947389345 1104 9 0.37357651356634847 1104 10 0.3952557591869663 1104 11 0.36762450260162893 1104 12 0.3674311531682406 1104 13 0.4458432083986068 1104 14 0.513548456171776 1104 15 0.4692096221435378 1104 16 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24 -0.6214521957822186 2216 25 -0.7777816961389874 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 68674.0 3 11.0 4 1212.0 5 10718.0 6 12757.0 7 10199.0 8 8551.0 9 7606.0 10 7081.0 11 6607.0 12 6307.0 13 6310.0 14 6340.0 15 6612.0 16 6947.0 17 7645.0 18 8389.0 19 9729.0 20 11785.0 21 15197.0 22 19563.0 23 24251.0 24 29853.0 25 35874.0 26 41930.0 27 49096.0 28 58684.0 29 73538.0 30 95597.0 31 127874.0 32 169187.0 33 228742.0 34 319576.0 35 453733.0 36 676637.0 37 1217602.0 38 3352244.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.88858817796252 17.25583447189351 12.685692860226347 26.16988448991762 2 18.234947103582048 20.078629641375482 35.75746688925183 25.92895636579064 3 19.49529606129428 23.32773178414301 27.61927114603358 29.55770100852913 4 14.05249538139909 16.06593521063132 35.203445435892036 34.67812397207755 5 16.663201728251025 34.39280720503234 31.878124093930193 17.06586697278644 6 34.12664397745625 35.3159768016785 15.440412157327568 15.116967063537675 7 30.841940507700414 29.69817347812564 20.025291544094532 19.434594470079418 8 27.96107939853317 31.34209493248162 18.858171978818973 21.838653690166236 9 27.70676886238738 14.816399865143485 17.918753400620652 39.55807787184848 10 17.35668291562257 24.933536796424217 29.092630730204522 28.61714955774869 11 37.672158558827206 21.29218695734839 21.2338265915474 19.801827892277004 12 26.301703413418977 23.03029350580066 26.678859799738152 23.989143281042214 13 30.240884669872088 19.170178458682376 23.943990966877017 26.644945904568523 14 24.889640423124526 19.51873352948488 23.62545843542029 31.966167611970302 15 25.95213353602804 25.740146648710745 21.814016577994686 26.493703237266537 16 26.685293785187408 25.429158994775165 22.096229329341703 25.789317890695724 17 26.287630728316312 24.451104783835035 23.25114532610598 26.01011916174267 18 27.75297395598712 23.04576750031978 23.991607854914328 25.209650688778773 19 28.326273819775665 23.647850349620406 23.28806680367675 24.73780902692718 20 27.213769442806164 23.381753174762732 23.275837378515916 26.128640003915187 21 27.547369858191427 23.537306257189627 23.238139913305993 25.677183971312957 22 26.751556934863302 23.34942103461613 23.869075938269273 26.029946092251294 23 25.49405622654117 22.749283192168456 24.36077480729294 27.395885773997442 24 25.911348664499062 24.31103322840115 23.643192389047492 26.134425718052295 25 25.905597861599016 23.578354732798037 23.905330255163154 26.61071715043979 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 385.0 1 385.0 2 772.5 3 1160.0 4 1160.0 5 1160.0 6 3016.0 7 4872.0 8 4872.0 9 4872.0 10 7198.5 11 9525.0 12 9525.0 13 9525.0 14 14882.5 15 20240.0 16 20240.0 17 20240.0 18 37050.5 19 53861.0 20 53861.0 21 53861.0 22 82320.0 23 110779.0 24 110779.0 25 110779.0 26 154702.0 27 198625.0 28 198625.0 29 198625.0 30 238097.0 31 277569.0 32 277569.0 33 277569.0 34 351769.5 35 425970.0 36 425970.0 37 425970.0 38 478863.0 39 531756.0 40 531756.0 41 531756.0 42 606121.5 43 680487.0 44 680487.0 45 680487.0 46 738064.5 47 795642.0 48 795642.0 49 795642.0 50 825703.5 51 855765.0 52 855765.0 53 855765.0 54 813478.0 55 771191.0 56 771191.0 57 771191.0 58 738042.5 59 704894.0 60 704894.0 61 704894.0 62 642690.5 63 580487.0 64 580487.0 65 580487.0 66 506811.0 67 433135.0 68 433135.0 69 433135.0 70 353964.0 71 274793.0 72 274793.0 73 274793.0 74 232654.5 75 190516.0 76 190516.0 77 190516.0 78 164956.0 79 139396.0 80 139396.0 81 139396.0 82 108636.0 83 77876.0 84 77876.0 85 77876.0 86 56950.5 87 36025.0 88 36025.0 89 36025.0 90 26151.0 91 16277.0 92 16277.0 93 16277.0 94 8762.0 95 1247.0 96 1247.0 97 1247.0 98 716.0 99 185.0 100 185.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.031838021493584154 2 0.0028501285616527295 3 0.008341839692642137 4 0.16561332403125523 5 0.021869523060876802 6 0.08725564318503674 7 0.05135792637436675 8 0.020924114562377357 9 0.038845166835403545 10 0.02553993252563934 11 0.03958202934158694 12 0.03158776630280489 13 0.006409313497180042 14 0.0036147972001449257 15 0.003225511347821626 16 0.0038511493247697856 17 0.0022940059154765873 18 0.003030868421659976 19 0.0 20 0.0021132660554693413 21 5.5612264617614234E-5 22 0.0 23 6.95153307720178E-5 24 0.0010566330277346706 25 0.14555119957045087 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 7192658.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 39.75261825593852 #Duplication Level Percentage of deduplicated Percentage of total 1 81.50572400398357 32.40065932004244 2 11.608035703323061 9.22899624031013 3 2.8176704051131334 3.3602932795655414 4 1.0913221683035634 1.7353165420325858 5 0.5721168400180614 1.1371571169515922 6 0.3929928452895963 0.9373496733667457 7 0.2750173012626956 0.7652860453612063 8 0.20715102883613343 0.6587836616518181 9 0.1561261718204394 0.5585781697325095 >10 1.0492266785183082 8.212191712020262 >50 0.13875619204366532 3.8708529675122456 >100 0.14320498152384276 12.07084116580189 >500 0.022960359455441122 6.3039359233669305 >1k 0.018150589488650202 14.413544893293334 >5k 0.0014745159739243587 4.015452304757116 >10k+ 7.021504637735041E-5 0.33076098423372685 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTACAAAGGGCAGGGACTTAATCAA 13288 0.1847439430597145 No Hit GAATAGGACCGCGGTTCTATTTTGT 10412 0.14475872479964988 No Hit GTTCAAAGCAGGCCCGAGCCGCCTG 9680 0.13458168037462645 No Hit GATTAAGAGGGACGGCCGGGGGCAT 9588 0.13330259828842134 No Hit GTATCTGATCGTCTTCGAACCTCCG 9575 0.1331218584284141 No Hit CTATTGGAGCTGGAATTACCGCGGC 9120 0.12679596332816045 No Hit GTATCAACGCAGAGTACTTTTTTTT 8924 0.12407096236189738 No Hit ATACAGGACTCTTTCGAGGCCCTGT 8709 0.12108180313870061 No Hit GTCCTATTCCATTATTCCTAGCTGC 8613 0.11974710878787785 No Hit GAACTACGACGGTATCTGATCGTCT 8312 0.11556228587540239 No Hit ATCAGATACCGTCGTAGTTCCGACC 8260 0.1148393264353734 No Hit CTCTTAATCATGGCCTCAGTTCCGA 7846 0.10908345704745033 No Hit GTCTTGCGCCGGTCCAAGAATTTCA 7837 0.1089583294520607 No Hit GTGTAGCGCGCGTGCAGCCCCGGAC 7802 0.10847172213665657 No Hit GGGTAGGCACACGCTGAGCCAGTCA 7783 0.10820756387972291 No Hit TCGTAGTTCCGACCATAAACGATGC 7647 0.10631674688272404 No Hit CTTTAATATACGCTATTGGAGCTGG 7567 0.10520450159037173 No Hit CTTATGACCCGCACTTACTGGGAAT 7406 0.10296610793951277 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 1.390306615440356E-5 0.0 5 0.0 0.0 0.0 2.780613230880712E-5 0.0 6 0.0 1.390306615440356E-5 0.0 2.780613230880712E-5 0.0 7 0.0 1.390306615440356E-5 0.0 2.780613230880712E-5 1.390306615440356E-5 8 0.0 1.390306615440356E-5 0.0 4.170919846321068E-5 1.390306615440356E-5 9 0.0 1.390306615440356E-5 0.0 8.341839692642136E-5 1.390306615440356E-5 10 1.390306615440356E-5 1.390306615440356E-5 0.0 8.341839692642136E-5 1.390306615440356E-5 11 1.390306615440356E-5 1.390306615440356E-5 0.0 9.732146308082492E-5 1.390306615440356E-5 12 1.390306615440356E-5 1.390306615440356E-5 0.0 1.1122452923522848E-4 2.780613230880712E-5 13 1.390306615440356E-5 1.390306615440356E-5 0.0 1.2512759538963204E-4 2.780613230880712E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4305 0.0 15.900586 1 GTATCAA 13765 0.0 13.099693 1 CGCTTAC 510 0.0 13.027947 18 CTCGACG 535 0.0 12.064583 13 CGGTAGC 545 0.0 12.020555 10 ACGCTTA 545 0.0 11.842968 17 ACCGTCC 540 0.0 11.780536 8 TAGGTCG 65 8.023219E-4 11.693422 5 CGAACGA 670 0.0 11.616899 16 GCTCGTA 895 0.0 11.457365 9 GGTAGCG 565 0.0 11.090916 11 CCGTCCA 545 0.0 10.9755945 9 AACCGCG 950 0.0 10.895275 7 TATAACG 80 3.706455E-4 10.7084875 2 TAGTACG 620 0.0 10.593342 2 CTCGTAG 995 0.0 10.591849 10 TCGACGC 610 0.0 10.581085 14 CGTCGTA 1460 0.0 10.469958 10 GCATCGC 1430 0.0 10.421128 14 AAGCTCG 1050 0.0 10.400252 7 >>END_MODULE