FastQCFastQC Report
Thu 2 Feb 2017
SRR4050224_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4050224_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences548385
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACGGAAGCAGTGGTATCAACGCA290725.301384975883732No Hit
CTTCCGTACTCTGCGTTGATACCAC169073.0830529646142764No Hit
GAGTACGGAAGCAGTGGTATCAACG148462.7072221158492664No Hit
TCCGTACTCTGCGTTGATACCACTG134572.4539329120964286No Hit
GCTTCCGTACTCTGCGTTGATACCA127212.3197206342259546No Hit
GCGTTGATACCACTGCTTCCGTACT67231.225963511036954No Hit
GTATCAACGCAGAGTACGGAAGCAG62941.1477338001586477No Hit
CCCATGTACTCTGCGTTGATACCAC62601.1415337764526747No Hit
GTACATGGGAAGCAGTGGTATCAAC61431.120198400758591No Hit
GTACATGGAAGCAGTGGTATCAACG59551.0859159167373287No Hit
TCCATGTACTCTGCGTTGATACCAC46940.855967978701095No Hit
TATCAACGCAGAGTACGGAAGCAGT44500.8114736909288182No Hit
ACTCTGCGTTGATACCACTGCTTCC38650.7047968124584005No Hit
CAGTGGTATCAACGCAGAGTACGGA32650.5953846294118184No Hit
GGTATCAACGCAGAGTACGGAAGCA30320.5528962316620623No Hit
ACGCAGAGTACGGAAGCAGTGGTAT28680.5229902349626632No Hit
ATACCACTGCTTCCGTACTCTGCGT26620.4854253854500032No Hit
CATGTACTCTGCGTTGATACCACTG25010.456066449665837No Hit
GTACGGGAAGCAGTGGTATCAACGC24520.4471311213836994No Hit
AAGCAGTGGTATCAACGCAGAGTAC21410.3904191398378876No Hit
GAGTACATGGAAGCAGTGGTATCAA21070.3842191161319146No Hit
GAAGCAGTGGTATCAACGCAGAGTA21020.38330734793985977No Hit
GTGGTATCAACGCAGAGTACGGAAG20450.3729131905504344No Hit
GAGTACATGGGAAGCAGTGGTATCA20370.3714543614431467No Hit
GTACTCTGCGTTGATACCACTGCTT17190.3134659044284581No Hit
GATACCACTGCTTCCGTACTCTGCG16440.29978938154763535No Hit
GTTGATACCACTGCTTCCGTACTCT15330.2795481276840176No Hit
CCACTGCTTCCGTACTCTGCGTTGA15220.27754223766149694No Hit
GTATCAACGCAGAGTACATGGGAAG14900.2717069212323459No Hit
ATCAACGCAGAGTACGGAAGCAGTG14570.26568925116478387No Hit
CCCGTACTCTGCGTTGATACCACTG14300.2607657029276877No Hit
TTCCCGTACTCTGCGTTGATACCAC14290.26058334928927673No Hit
GCGTTGATACCACTGCTTCCCATGT13440.24508329002434423No Hit
CGGAAGCAGTGGTATCAACGCAGAG13350.2434421072786455No Hit
CTGCTTCCGTACTCTGCGTTGATAC13340.24325975364023453No Hit
GCTTCCCATGTACTCTGCGTTGATA13120.23924797359519318No Hit
GAGTACGGGAAGCAGTGGTATCAAC12480.22757734073689104No Hit
CTTCCCGTACTCTGCGTTGATACCA12380.22575380435278136No Hit
GCTTCCATGTACTCTGCGTTGATAC12080.22028319520045223No Hit
CATGGAAGCAGTGGTATCAACGCAG11740.21408317149447922No Hit
TATCAACGCAGAGTACATGGGAAGC10860.19803605131431387No Hit
CAGTGGTATCAACGCAGAGTACATG10300.1878242475632995No Hit
GCGTTGATACCACTGCTTCCATGTA10110.18435952843349107No Hit
GTATCAACGCAGAGTACATGGAAGC9960.18162422385732652No Hit
GCAGAGTACGGAAGCAGTGGTATCA9760.1779771510891071No Hit
AAAAAGTACTCTGCGTTGATACCAC9750.17779479745069615No Hit
GTATCAACGCAGAGTACGGGAAGCA9710.17706538289705226No Hit
GTATCAACGCAGAGTACTTTTTTTT9650.17597126106658642No Hit
CACTGCTTCCGTACTCTGCGTTGAT8900.16229473818576365No Hit
ACGCAGAGTACATGGGAAGCAGTGG8510.1551829462877358No Hit
GCTTCCCGTACTCTGCGTTGATACC8470.15445353173409193No Hit
GCCCATGTACTCTGCGTTGATACCA8380.1528123489883932No Hit
GCAGTGGTATCAACGCAGAGTACGG8320.15171822715792738No Hit
CCCCATGTACTCTGCGTTGATACCA8300.15135351988110543No Hit
TGATACCACTGCTTCCGTACTCTGC8020.14624761800559827No Hit
GTACTTTTTTTTTTTTTTTTTTTTT7650.13950053338439236No Hit
CTGCGTTGATACCACTGCTTCCGTA7560.13785935063869362No Hit
GGTATCAACGCAGAGTACATGGGAA7380.13457698514729616No Hit
TATCAACGCAGAGTACATGGAAGCA7240.13202403420954256No Hit
TACCACTGCTTCCGTACTCTGCGTT7180.13092991237907675No Hit
AAAGTACTCTGCGTTGATACCACTG7030.1281946078029122No Hit
GCGTTGATACCACTGCTTCCCGTAC6970.12710048597244636No Hit
CCATGTACTCTGCGTTGATACCACT6730.12272399865058307No Hit
ACGCAGAGTACATGGAAGCAGTGGT6430.11725338949825397No Hit
TATCAACGCAGAGTACGGGAAGCAG5760.10503569572471894No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGAGTG250.005988511719.021185
AAGACGA250.005988511719.021186
TTCGAAG350.002210360716.238362
TATTAGC508.916224E-515.1558032
TCGCCTC456.695583E-414.794250510
CACTACC456.7375985E-414.78205318
TACGAAA400.00537617714.2085662
TGCTGGA400.00537617714.2085662
AGCCCCG759.608266E-713.93736416
AAAAGTA1700.013.9299662
AACTTTA551.9516927E-413.82217916
GTATATA551.9853194E-413.7943781
TGGAGAG500.00148485413.3148255
TGCCCTC500.00148485413.3148255
TCACTAC500.001494114613.30384717
TTTGAAC1001.0433723E-813.2613283
AAAAAGT1800.013.1717151
GCAGTTA655.425112E-513.15765315
TTGCGCG655.425112E-513.15765318
AGTTAAA655.425112E-513.15765317
Produced by FastQC (version 0.11.5)