##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR4050224_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 548385 Sequences flagged as poor quality 0 Sequence length 25 %GC 50 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.429160170318298 31.0 31.0 34.0 27.0 34.0 2 30.515608559679787 31.0 31.0 34.0 27.0 34.0 3 30.64722412173929 31.0 31.0 34.0 27.0 34.0 4 34.141329540377654 37.0 35.0 37.0 30.0 37.0 5 34.14972327835371 37.0 35.0 37.0 30.0 37.0 6 34.1536493521887 37.0 35.0 37.0 30.0 37.0 7 34.03465266190724 37.0 35.0 37.0 30.0 37.0 8 33.968655233093536 37.0 35.0 37.0 30.0 37.0 9 35.439702034154834 39.0 37.0 39.0 30.0 39.0 10 35.392311970604595 39.0 37.0 39.0 30.0 39.0 11 35.22622062966711 39.0 37.0 39.0 29.0 39.0 12 35.181447340828065 39.0 37.0 39.0 29.0 39.0 13 35.15466870902742 39.0 37.0 39.0 29.0 39.0 14 36.32763295859661 40.0 37.0 41.0 28.0 41.0 15 36.26742161072969 40.0 37.0 41.0 28.0 41.0 16 36.14948621862378 40.0 37.0 41.0 27.0 41.0 17 36.20028447167592 40.0 37.0 41.0 28.0 41.0 18 36.119609398506526 40.0 37.0 41.0 28.0 41.0 19 36.21047986359948 40.0 37.0 41.0 28.0 41.0 20 36.20047411945987 40.0 37.0 41.0 28.0 41.0 21 36.141705188872784 40.0 37.0 41.0 28.0 41.0 22 36.090434639897154 40.0 37.0 41.0 28.0 41.0 23 35.99615051469314 40.0 37.0 41.0 27.0 41.0 24 35.91080901191681 40.0 37.0 41.0 27.0 41.0 25 35.68989304959107 40.0 36.0 41.0 27.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.46525481790243717 1101 2 0.5662947834197674 1101 3 0.3552678695946234 1101 4 0.3641757766541289 1101 5 0.40699663588989665 1101 6 0.359812768217175 1101 7 0.493886348676952 1101 8 0.45378220231361865 1101 9 0.41295323278551166 1101 10 0.5189969828667813 1101 11 0.34398372900516705 1101 12 0.4009296183713573 1101 13 0.5789534404092578 1101 14 0.6013578188238569 1101 15 0.6500446513446789 1101 16 0.6232926756460984 1101 17 0.7158459020363779 1101 18 0.5388332136350158 1101 19 0.5206672915888291 1101 20 0.5297540447280724 1101 21 0.3003355671194825 1101 22 0.4197487185762654 1101 23 0.5273291765593697 1101 24 0.4785718547100899 1101 25 0.52049200665612 1102 1 0.4744664646451895 1102 2 0.5355887441968328 1102 3 0.4765969079000243 1102 4 0.3830165811797528 1102 5 0.2916002149331547 1102 6 0.4525876819095558 1102 7 0.3361562885421563 1102 8 0.33686425891028904 1102 9 0.49779048599192777 1102 10 0.5657945246673961 1102 11 0.5980713766192522 1102 12 0.7734154758037164 1102 13 0.7125619239695524 1102 14 0.6691975869979601 1102 15 0.6260732467253263 1102 16 0.7292959233209046 1102 17 0.6973896037255685 1102 18 0.6082357602873358 1102 19 0.6595713993693906 1102 20 0.6256825694783927 1102 21 0.5691021107881653 1102 22 0.7936752366332058 1102 23 0.6101877904887303 1102 24 0.690558547164521 1102 25 0.6672842676846855 1103 1 0.434431744065904 1103 2 0.5109237537335716 1103 3 0.47870983788943633 1103 4 0.5549307652216626 1103 5 0.5056995764856609 1103 6 0.5594366751269675 1103 7 0.3808737188277078 1103 8 0.4157573120207019 1103 9 0.5884033662173493 1103 10 0.581079411262607 1103 11 0.3918249355602086 1103 12 0.23920640505237856 1103 13 0.42655931013646864 1103 14 0.44441936306654384 1103 15 0.5097756221807472 1103 16 0.5331752301493395 1103 17 0.3960107333517797 1103 18 0.5578958151419329 1103 19 0.6329729555953492 1103 20 0.6860030495621956 1103 21 0.6515781710466442 1103 22 0.5320814510947898 1103 23 0.8240032525677492 1103 24 0.7831244599747507 1103 25 0.893406935099712 1104 1 0.3946662869681248 1104 2 0.566897889415646 1104 3 0.5045617329911138 1104 4 0.5485314584151979 1104 5 0.6163372400010729 1104 6 0.50475296020592 1104 7 0.6816721957895808 1104 8 0.5694152123151213 1104 9 0.713513618773348 1104 10 0.7925935049555406 1104 11 0.783283623461621 1104 12 0.6681369863162772 1104 13 0.8938375937992191 1104 14 0.9291082334592247 1104 15 0.8090259047999737 1104 16 0.9371716523762572 1104 17 0.9017407605577645 1104 18 0.9248147686433086 1104 19 1.0421403779837632 1104 20 1.0302503507038523 1104 21 0.9695884695670856 1104 22 1.0705440646083346 1104 23 1.217508600593142 1104 24 1.4494971198254305 1104 25 1.3135701088825869 1105 1 -0.09168788350427448 1105 2 0.060586464915264315 1105 3 -0.11793409592756987 1105 4 -0.2382851845037024 1105 5 -0.11676226921184707 1105 6 0.08845925518966169 1105 7 0.1555268229497102 1105 8 0.013486187678346084 1105 9 -0.07128294206354724 1105 10 0.016330915596974194 1105 11 -0.02908774282629878 1105 12 -0.06570083473520327 1105 13 0.12296696430084353 1105 14 0.12752992064639557 1105 15 0.18211238318951217 1105 16 0.1955777512887309 1105 17 0.1811027373458458 1105 18 0.1785519966096487 1105 19 0.21585525869692646 1105 20 0.08725868068813725 1105 21 -0.06996525517808294 1105 22 0.045583035295074126 1105 23 0.05817938353860086 1105 24 0.016252688371217516 1105 25 0.06570588519084453 1106 1 0.47828399339896066 1106 2 0.6020680326435617 1106 3 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15 -0.04940259943899861 1107 16 0.02927739157380671 1107 17 0.013452510295465459 1107 18 0.08821276191400784 1107 19 -0.11449967700833952 1107 20 -0.06633404890541783 1107 21 0.03778741796375584 1107 22 -0.15645060501697117 1107 23 -0.09185814606934173 1107 24 -0.20798409723659006 1107 25 0.058593987031670736 1108 1 0.28911146128049126 1108 2 0.31400164080405446 1108 3 0.24090159134365763 1108 4 0.3055108204044714 1108 5 0.4066368972285517 1108 6 0.34856902154667324 1108 7 0.3417735346327504 1108 8 0.40237631300902876 1108 9 0.5309245516639862 1108 10 0.4733821206084201 1108 11 0.27432913935217584 1108 12 0.0573256186897666 1108 13 0.2822394617302777 1108 14 0.41635876792160076 1108 15 0.21570996886379845 1108 16 0.3100950122635098 1108 17 0.360497583164026 1108 18 0.4857449720375939 1108 19 0.1200255790491056 1108 20 0.2993222971023499 1108 21 0.2797692313103326 1108 22 0.07484812891102166 1108 23 0.3761991379219083 1108 24 0.509380792149237 1108 25 0.43038895301067015 1109 1 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-0.7780082506259518 2216 4 -0.6421198394997916 2216 5 -0.7868762570937591 2216 6 -0.8454462298055816 2216 7 -0.6913537116315851 2216 8 -0.7420131555987481 2216 9 -1.1100289615895207 2216 10 -0.959049876237664 2216 11 -0.9886705246767278 2216 12 -1.0082606508780714 2216 13 -0.8560988078573928 2216 14 -1.0591184412123553 2216 15 -0.9458177450326346 2216 16 -1.1206112584944776 2216 17 -1.3315557705341945 2216 18 -1.403534574513003 2216 19 -1.0898261845512138 2216 20 -1.0853956049502997 2216 21 -1.3368728677279975 2216 22 -1.3114492092308723 2216 23 -1.443995576546051 2216 24 -1.5921419875591027 2216 25 -1.651434266683438 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 14798.0 3 0.0 4 114.0 5 849.0 6 1477.0 7 1449.0 8 1248.0 9 1070.0 10 1058.0 11 1474.0 12 1486.0 13 1380.0 14 670.0 15 635.0 16 675.0 17 780.0 18 879.0 19 1052.0 20 1546.0 21 1953.0 22 2283.0 23 2639.0 24 3027.0 25 3508.0 26 3791.0 27 4181.0 28 4785.0 29 5660.0 30 6996.0 31 9016.0 32 11768.0 33 15502.0 34 21155.0 35 30286.0 36 45851.0 37 85632.0 38 257712.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 49.96264055463705 14.082227317153375 12.964001571106259 22.991130557103318 2 11.31751981249943 21.280037937858516 39.720025899884185 27.682416349757872 3 21.024300551970203 29.40782681342094 27.134461108537117 22.433411526071744 4 13.222716824169009 14.140183164884885 33.458787900901235 39.17831211004488 5 17.4250508477668 33.081055444587335 29.23596099999088 20.257932707654984 6 36.997939844931814 29.970785577819665 14.087154230616934 18.944120346631582 7 27.41508690225048 32.19598811618112 21.304008233997358 19.084916747571043 8 26.264143368129062 33.92054164537557 23.295861011752685 16.51945397474268 9 29.0940474105214 20.146004846149353 15.08363986687686 35.676307876452384 10 14.25855652046997 25.29628548493031 29.49116981682843 30.95398817777129 11 32.222456674709406 25.68013137494151 18.38386803790791 23.71354391244117 12 26.719806656984847 22.885585823468148 25.410743886517334 24.983863633029678 13 31.58395512127156 19.642693457018716 27.28784351110052 21.4855079106092 14 31.90530309973787 18.677616263083607 22.82422598207248 26.592854655106045 15 33.237634816660346 19.619295900913535 25.39915631307715 21.743912969348976 16 28.278979231619406 21.655128677447678 30.049909364008435 20.01598272692448 17 24.5362563237774 28.379096707969794 27.455825207126622 19.628821761126183 18 26.641897601237595 18.929639113372545 36.24721164720395 18.18125163818591 19 24.945965715039957 31.98591350481313 19.927147668047084 23.14097311209983 20 20.201379558123463 28.96118870030814 28.23658157008599 22.600850171482413 21 21.757653528872595 32.14115490375802 21.492208982584785 24.6089825847846 22 20.333226412500576 30.146909224212987 18.87971406314439 30.64015030014205 23 24.28981777320729 24.49860594028915 19.282751237789377 31.92882504871418 24 19.049591396509413 28.33045291850473 20.3255265844267 32.294429100559164 25 26.379745165026907 28.475205642146012 19.651471386050055 25.49357780677703 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 263.0 1 263.0 2 298.5 3 334.0 4 334.0 5 334.0 6 831.5 7 1329.0 8 1329.0 9 1329.0 10 1386.5 11 1444.0 12 1444.0 13 1444.0 14 1732.5 15 2021.0 16 2021.0 17 2021.0 18 3009.5 19 3998.0 20 3998.0 21 3998.0 22 5146.0 23 6294.0 24 6294.0 25 6294.0 26 8583.5 27 10873.0 28 10873.0 29 10873.0 30 13368.5 31 15864.0 32 15864.0 33 15864.0 34 18337.0 35 20810.0 36 20810.0 37 20810.0 38 23287.0 39 25764.0 40 25764.0 41 25764.0 42 31303.0 43 36842.0 44 36842.0 45 36842.0 46 64666.0 47 92490.0 48 92490.0 49 92490.0 50 142436.0 51 192382.0 52 192382.0 53 192382.0 54 122877.0 55 53372.0 56 53372.0 57 53372.0 58 40650.0 59 27928.0 60 27928.0 61 27928.0 62 24633.5 63 21339.0 64 21339.0 65 21339.0 66 17943.5 67 14548.0 68 14548.0 69 14548.0 70 11388.0 71 8228.0 72 8228.0 73 8228.0 74 6813.5 75 5399.0 76 5399.0 77 5399.0 78 4581.5 79 3764.0 80 3764.0 81 3764.0 82 2793.5 83 1823.0 84 1823.0 85 1823.0 86 1361.0 87 899.0 88 899.0 89 899.0 90 613.5 91 328.0 92 328.0 93 328.0 94 183.0 95 38.0 96 38.0 97 38.0 98 24.5 99 11.0 100 11.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.18235363841097038 2 0.021882436609316446 3 0.030453057614632056 4 0.044676641410687744 5 0.032823654913974674 6 0.0671061389352371 7 0.07531205266373077 8 0.07750029632466242 9 0.058535517929921496 10 0.04832371417890716 11 0.6163552978290799 12 0.5530785853004732 13 0.5315608559679788 14 0.5206196376633204 15 0.5328373314368554 16 0.5098607729970732 17 0.5151490285109914 18 0.5127784312116488 19 0.5284608441149922 20 0.5193431621944437 21 0.5260902468156495 22 0.5124137239348268 23 0.5208019913017314 24 0.5007430910765247 25 0.5063960538672647 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 548385.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 36.52208846215743 #Duplication Level Percentage of deduplicated Percentage of total 1 89.23275499450932 32.58966571631489 2 6.15565133473542 4.496344851788089 3 1.4154374779259744 1.550841983443963 4 0.6939906593812659 1.0138395301533423 5 0.4186514093289599 0.7645011903159578 6 0.2963718293970786 0.6494470902558921 7 0.218926054916435 0.5596945721030422 8 0.15956490437977383 0.4662114842571033 9 0.1248338168753686 0.41032725326918756 >10 0.9678460035250012 7.11483406463153 >50 0.15898884168739788 4.068103477673627 >100 0.12237626931143546 8.944552249911327 >500 0.013040094270890663 3.641492058901869 >1k 0.0165508888822843 12.046414751191218 >5k 0.002507710436709743 5.747073106038539 >10k+ 0.002507710436709743 15.936656619750469 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTACGGAAGCAGTGGTATCAACGCA 29072 5.301384975883732 No Hit CTTCCGTACTCTGCGTTGATACCAC 16907 3.0830529646142764 No Hit GAGTACGGAAGCAGTGGTATCAACG 14846 2.7072221158492664 No Hit TCCGTACTCTGCGTTGATACCACTG 13457 2.4539329120964286 No Hit GCTTCCGTACTCTGCGTTGATACCA 12721 2.3197206342259546 No Hit GCGTTGATACCACTGCTTCCGTACT 6723 1.225963511036954 No Hit GTATCAACGCAGAGTACGGAAGCAG 6294 1.1477338001586477 No Hit CCCATGTACTCTGCGTTGATACCAC 6260 1.1415337764526747 No Hit GTACATGGGAAGCAGTGGTATCAAC 6143 1.120198400758591 No Hit GTACATGGAAGCAGTGGTATCAACG 5955 1.0859159167373287 No Hit TCCATGTACTCTGCGTTGATACCAC 4694 0.855967978701095 No Hit TATCAACGCAGAGTACGGAAGCAGT 4450 0.8114736909288182 No Hit ACTCTGCGTTGATACCACTGCTTCC 3865 0.7047968124584005 No Hit CAGTGGTATCAACGCAGAGTACGGA 3265 0.5953846294118184 No Hit GGTATCAACGCAGAGTACGGAAGCA 3032 0.5528962316620623 No Hit ACGCAGAGTACGGAAGCAGTGGTAT 2868 0.5229902349626632 No Hit ATACCACTGCTTCCGTACTCTGCGT 2662 0.4854253854500032 No Hit CATGTACTCTGCGTTGATACCACTG 2501 0.456066449665837 No Hit GTACGGGAAGCAGTGGTATCAACGC 2452 0.4471311213836994 No Hit AAGCAGTGGTATCAACGCAGAGTAC 2141 0.3904191398378876 No Hit GAGTACATGGAAGCAGTGGTATCAA 2107 0.3842191161319146 No Hit GAAGCAGTGGTATCAACGCAGAGTA 2102 0.38330734793985977 No Hit GTGGTATCAACGCAGAGTACGGAAG 2045 0.3729131905504344 No Hit GAGTACATGGGAAGCAGTGGTATCA 2037 0.3714543614431467 No Hit GTACTCTGCGTTGATACCACTGCTT 1719 0.3134659044284581 No Hit GATACCACTGCTTCCGTACTCTGCG 1644 0.29978938154763535 No Hit GTTGATACCACTGCTTCCGTACTCT 1533 0.2795481276840176 No Hit CCACTGCTTCCGTACTCTGCGTTGA 1522 0.27754223766149694 No Hit GTATCAACGCAGAGTACATGGGAAG 1490 0.2717069212323459 No Hit ATCAACGCAGAGTACGGAAGCAGTG 1457 0.26568925116478387 No Hit CCCGTACTCTGCGTTGATACCACTG 1430 0.2607657029276877 No Hit TTCCCGTACTCTGCGTTGATACCAC 1429 0.26058334928927673 No Hit GCGTTGATACCACTGCTTCCCATGT 1344 0.24508329002434423 No Hit CGGAAGCAGTGGTATCAACGCAGAG 1335 0.2434421072786455 No Hit CTGCTTCCGTACTCTGCGTTGATAC 1334 0.24325975364023453 No Hit GCTTCCCATGTACTCTGCGTTGATA 1312 0.23924797359519318 No Hit GAGTACGGGAAGCAGTGGTATCAAC 1248 0.22757734073689104 No Hit CTTCCCGTACTCTGCGTTGATACCA 1238 0.22575380435278136 No Hit GCTTCCATGTACTCTGCGTTGATAC 1208 0.22028319520045223 No Hit CATGGAAGCAGTGGTATCAACGCAG 1174 0.21408317149447922 No Hit TATCAACGCAGAGTACATGGGAAGC 1086 0.19803605131431387 No Hit CAGTGGTATCAACGCAGAGTACATG 1030 0.1878242475632995 No Hit GCGTTGATACCACTGCTTCCATGTA 1011 0.18435952843349107 No Hit GTATCAACGCAGAGTACATGGAAGC 996 0.18162422385732652 No Hit GCAGAGTACGGAAGCAGTGGTATCA 976 0.1779771510891071 No Hit AAAAAGTACTCTGCGTTGATACCAC 975 0.17779479745069615 No Hit GTATCAACGCAGAGTACGGGAAGCA 971 0.17706538289705226 No Hit GTATCAACGCAGAGTACTTTTTTTT 965 0.17597126106658642 No Hit CACTGCTTCCGTACTCTGCGTTGAT 890 0.16229473818576365 No Hit ACGCAGAGTACATGGGAAGCAGTGG 851 0.1551829462877358 No Hit GCTTCCCGTACTCTGCGTTGATACC 847 0.15445353173409193 No Hit GCCCATGTACTCTGCGTTGATACCA 838 0.1528123489883932 No Hit GCAGTGGTATCAACGCAGAGTACGG 832 0.15171822715792738 No Hit CCCCATGTACTCTGCGTTGATACCA 830 0.15135351988110543 No Hit TGATACCACTGCTTCCGTACTCTGC 802 0.14624761800559827 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 765 0.13950053338439236 No Hit CTGCGTTGATACCACTGCTTCCGTA 756 0.13785935063869362 No Hit GGTATCAACGCAGAGTACATGGGAA 738 0.13457698514729616 No Hit TATCAACGCAGAGTACATGGAAGCA 724 0.13202403420954256 No Hit TACCACTGCTTCCGTACTCTGCGTT 718 0.13092991237907675 No Hit AAAGTACTCTGCGTTGATACCACTG 703 0.1281946078029122 No Hit GCGTTGATACCACTGCTTCCCGTAC 697 0.12710048597244636 No Hit CCATGTACTCTGCGTTGATACCACT 673 0.12272399865058307 No Hit ACGCAGAGTACATGGAAGCAGTGGT 643 0.11725338949825397 No Hit TATCAACGCAGAGTACGGGAAGCAG 576 0.10503569572471894 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 1.823536384109704E-4 0.0 9 0.0 0.0 0.0 1.823536384109704E-4 0.0 10 0.0 0.0 0.0 3.647072768219408E-4 0.0 11 0.0 0.0 0.0 3.647072768219408E-4 0.0 12 0.0 0.0 0.0 7.294145536438816E-4 0.0 13 0.0 0.0 0.0 0.0016411827456987336 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGAGTG 25 0.0059885117 19.02118 5 AAGACGA 25 0.0059885117 19.02118 6 TTCGAAG 35 0.0022103607 16.23836 2 TATTAGC 50 8.916224E-5 15.155803 2 TCGCCTC 45 6.695583E-4 14.7942505 10 CACTACC 45 6.7375985E-4 14.782053 18 TACGAAA 40 0.005376177 14.208566 2 TGCTGGA 40 0.005376177 14.208566 2 AGCCCCG 75 9.608266E-7 13.937364 16 AAAAGTA 170 0.0 13.929966 2 AACTTTA 55 1.9516927E-4 13.822179 16 GTATATA 55 1.9853194E-4 13.794378 1 TGGAGAG 50 0.001484854 13.314825 5 TGCCCTC 50 0.001484854 13.314825 5 TCACTAC 50 0.0014941146 13.303847 17 TTTGAAC 100 1.0433723E-8 13.261328 3 AAAAAGT 180 0.0 13.171715 1 GCAGTTA 65 5.425112E-5 13.157653 15 TTGCGCG 65 5.425112E-5 13.157653 18 AGTTAAA 65 5.425112E-5 13.157653 17 >>END_MODULE