FastQCFastQC Report
Thu 2 Feb 2017
SRR4050224_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4050224_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences548385
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACGGAAGCAGTGGTATCAACGCA268774.901118739571651No Hit
CTTCCGTACTCTGCGTTGATACCAC159772.9134640808920738No Hit
GAGTACGGAAGCAGTGGTATCAACG150942.7524458181751874No Hit
TCCGTACTCTGCGTTGATACCACTG133062.426397512696372No Hit
GCTTCCGTACTCTGCGTTGATACCA124612.272308688239102No Hit
CCCATGTACTCTGCGTTGATACCAC64501.176180967750759No Hit
GCGTTGATACCACTGCTTCCGTACT64191.170528004960019No Hit
GTATCAACGCAGAGTACGGAAGCAG63831.163963273977224No Hit
GTACATGGGAAGCAGTGGTATCAAC53060.9675684054086089No Hit
TCCATGTACTCTGCGTTGATACCAC52680.9606389671489921No Hit
TATCAACGCAGAGTACGGAAGCAGT52030.9487859806522789No Hit
GTACATGGAAGCAGTGGTATCAACG50150.9145034966310165No Hit
ACTCTGCGTTGATACCACTGCTTCC39880.7272263099829499No Hit
CAGTGGTATCAACGCAGAGTACGGA35880.6542848546185618No Hit
ACGCAGAGTACGGAAGCAGTGGTAT34110.6220082606198201No Hit
GGTATCAACGCAGAGTACGGAAGCA31810.5800669237852968No Hit
CATGTACTCTGCGTTGATACCACTG28940.5277314295613483No Hit
ATACCACTGCTTCCGTACTCTGCGT28490.5195255158328547No Hit
AAGCAGTGGTATCAACGCAGAGTAC26170.4772194717215095No Hit
GTGGTATCAACGCAGAGTACGGAAG23240.4237898556670952No Hit
GAAGCAGTGGTATCAACGCAGAGTA22850.4166780637690674No Hit
GTACGGGAAGCAGTGGTATCAACGC21200.38658971343125725No Hit
GAGTACATGGGAAGCAGTGGTATCA19940.36361315499147495No Hit
GAGTACATGGAAGCAGTGGTATCAA19440.35449547307092644No Hit
ATCAACGCAGAGTACGGAAGCAGTG17640.3216718181569518No Hit
CGGAAGCAGTGGTATCAACGCAGAG16480.30051879610127924No Hit
GATACCACTGCTTCCGTACTCTGCG16250.2963246624178269No Hit
CCCGTACTCTGCGTTGATACCACTG15190.27699517674626406No Hit
TTCCCGTACTCTGCGTTGATACCAC15000.27353045761645556No Hit
CCACTGCTTCCGTACTCTGCGTTGA14840.2706127994018801No Hit
GTATCAACGCAGAGTACATGGGAAG14790.26970103120982525No Hit
GTTGATACCACTGCTTCCGTACTCT14640.26696572663366064No Hit
GTACTCTGCGTTGATACCACTGCTT14220.2593068738203999No Hit
GCTTCCCATGTACTCTGCGTTGATA13690.24964213098461846No Hit
GCGTTGATACCACTGCTTCCCATGT13590.24781859460050876No Hit
CATGGAAGCAGTGGTATCAACGCAG13370.2438068145554674No Hit
GTATCAACGCAGAGTACTTTTTTTT13190.24052444906406995No Hit
GAGTACGGGAAGCAGTGGTATCAAC13110.23906561995678222No Hit
GCTTCCATGTACTCTGCGTTGATAC13020.23742443721108344No Hit
CTGCTTCCGTACTCTGCGTTGATAC12690.23140676714352143No Hit
GTACTTTTTTTTTTTTTTTTTTTTT11530.2102537450878489No Hit
CAGTGGTATCAACGCAGAGTACATG11280.2056949041275746No Hit
TATCAACGCAGAGTACATGGGAAGC11100.20241253863617714No Hit
CTTCCCGTACTCTGCGTTGATACCA10970.20004194133683453No Hit
GCAGAGTACGGAAGCAGTGGTATCA10600.19329485671562863No Hit
AAAAAGTACTCTGCGTTGATACCAC10500.1914713203315189No Hit
GCGTTGATACCACTGCTTCCATGTA10380.18928307667058727No Hit
GCAGTGGTATCAACGCAGAGTACGG10330.18837130847853242No Hit
TATCAACGCAGAGTACTTTTTTTTT10240.18673012573283368No Hit
ACGCAGAGTACATGGGAAGCAGTGG9570.17451243195929866No Hit
GTATCAACGCAGAGTACGGGAAGCA9400.17141242010631216No Hit
GTATCAACGCAGAGTACATGGAAGC8860.16156532363211978No Hit
CCCCATGTACTCTGCGTTGATACCA8690.15846531177913328No Hit
GCCCATGTACTCTGCGTTGATACCA8660.15791825086390038No Hit
CACTGCTTCCGTACTCTGCGTTGAT8440.15390647081885903No Hit
GCTTCCCGTACTCTGCGTTGATACC7670.13986524066121428No Hit
AAAGTACTCTGCGTTGATACCACTG7590.13840641155392655No Hit
TGATACCACTGCTTCCGTACTCTGC7480.13640052153140586No Hit
GCGTTGATACCACTGCTTCCCGTAC7360.1342122778704742No Hit
TATCAACGCAGAGTACATGGAAGCA7350.13402992423206325No Hit
CTGCGTTGATACCACTGCTTCCGTA7190.1311122660174877No Hit
TATCAACGCAGAGTACGGGAAGCAG7140.13020049782543286No Hit
TACGGAAGCAGTGGTATCAACGCAG7130.1300181441870219No Hit
TCAACGCAGAGTACGGAAGCAGTGG7080.12910637599496702No Hit
ACGCAGAGTACATGGAAGCAGTGGT6970.12710048597244636No Hit
GGTATCAACGCAGAGTACATGGGAA6940.12655342505721345No Hit
TACCACTGCTTCCGTACTCTGCGTT6640.12108281590488434No Hit
CCATGTACTCTGCGTTGATACCACT6580.11998869407441853No Hit
GAGTACTTTTTTTTTTTTTTTTTTT6160.11232984126115778No Hit
GGGAAGCAGTGGTATCAACGCAGAG6040.11014159760022611No Hit
GGTATCAACGCAGAGTACTTTTTTT5680.10357686661743118No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCCGT250.005920628619.0587651
ACCCGCA250.006043777318.990977
TTAGCTC555.80967E-717.296074
AACGCCA350.002178663616.27352115
CGCATCG755.8687874E-815.19000613
GCATCGC755.8687874E-815.19000614
GCTCGCT456.6975516E-414.79369419
CGCTCGC456.786216E-414.76806218
CGCCAGT902.2955646E-914.76806218
GGTCGCT456.79091E-414.76671415
CATCGCC851.7254933E-814.51853315
GTATTAG602.4887124E-514.2940731
AAGATCC400.00522628714.2692584
GATCCAA400.00528359314.2458276
AGCTCTA602.5693693E-514.2458266
GTCGTAG602.5782265E-514.24062911
CGTCGTA602.5782265E-514.24062910
TCGCCAG954.9258233E-913.98951817
TTACCGC903.657442E-813.71194816
GCCGCAT852.7098758E-713.402945511
Produced by FastQC (version 0.11.5)