##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4050224_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	548385
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.94111071601156	31.0	31.0	34.0	28.0	34.0
2	30.789290370816122	31.0	31.0	34.0	27.0	34.0
3	31.191527849959428	33.0	31.0	34.0	27.0	34.0
4	34.75623330324498	37.0	35.0	37.0	32.0	37.0
5	34.67256945394203	37.0	35.0	37.0	32.0	37.0
6	34.676579410450685	37.0	35.0	37.0	32.0	37.0
7	34.57654020441843	37.0	35.0	37.0	32.0	37.0
8	34.58980825514921	37.0	35.0	37.0	32.0	37.0
9	36.20915415264823	39.0	37.0	39.0	32.0	39.0
10	36.14812403694485	39.0	37.0	39.0	32.0	39.0
11	36.13725940716832	39.0	37.0	39.0	32.0	39.0
12	36.07553817117536	39.0	37.0	39.0	32.0	39.0
13	35.992151499402794	39.0	37.0	39.0	31.0	39.0
14	37.22304038221323	40.0	38.0	41.0	32.0	41.0
15	37.1919782634463	40.0	38.0	41.0	32.0	41.0
16	37.05317796803341	40.0	37.0	41.0	31.0	41.0
17	37.05826198747231	40.0	37.0	41.0	31.0	41.0
18	36.984751588756076	40.0	37.0	41.0	31.0	41.0
19	37.10379386744714	40.0	37.0	41.0	31.0	41.0
20	37.08456285274032	40.0	37.0	41.0	31.0	41.0
21	37.04721682759375	40.0	37.0	41.0	31.0	41.0
22	37.01084821794907	40.0	37.0	41.0	31.0	41.0
23	36.93485416267768	40.0	37.0	41.0	31.0	41.0
24	36.855513918141455	40.0	37.0	41.0	31.0	41.0
25	36.61426552513289	40.0	37.0	41.0	30.0	41.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.10314423564778608
1101	2	0.52089454731383
1101	3	0.2561709594449759
1101	4	0.33390893062326654
1101	5	0.28641509713182955
1101	6	0.22660552373287146
1101	7	0.38378714356815635
1101	8	0.2621385105204368
1101	9	0.31706580562325826
1101	10	0.2704897756769
1101	11	0.21365258004547627
1101	12	0.3148314890483732
1101	13	0.42040945194673895
1101	14	0.5114186134848637
1101	15	0.5376112320040818
1101	16	0.6165585189656397
1101	17	0.6412550336634126
1101	18	0.55063330926437
1101	19	0.520470429492228
1101	20	0.37269971293535775
1101	21	0.11111569834765334
1101	22	0.5892561511958903
1101	23	0.6406217294026746
1101	24	0.6864258779897554
1101	25	0.46787571143812556
1102	1	0.4496288917045028
1102	2	0.7125898731704723
1102	3	0.6174203241020884
1102	4	0.5592543261200902
1102	5	0.562959227883745
1102	6	0.5139752371849795
1102	7	0.45908435202002806
1102	8	0.5219013378457902
1102	9	0.5662637883683388
1102	10	0.7373364286575637
1102	11	0.5920875572391395
1102	12	0.5606118221330689
1102	13	0.43649105307682134
1102	14	0.7292537609834255
1102	15	0.7734323590528618
1102	16	0.8497623633676881
1102	17	0.7835421360377737
1102	18	0.788566745132691
1102	19	0.7584368701858537
1102	20	0.749992723173456
1102	21	0.7219260988282059
1102	22	0.931174589671599
1102	23	0.7270052586746516
1102	24	0.6195036439591419
1102	25	0.7980196784861064
1103	1	0.1374161563657097
1103	2	0.5597426388245097
1103	3	0.26097800872240384
1103	4	0.42037690379677883
1103	5	0.3004174791608847
1103	6	0.3326026321788902
1103	7	0.4654002995018871
1103	8	0.43662054658206273
1103	9	0.37473606656372027
1103	10	0.20299682941267605
1103	11	0.1618036414496018
1103	12	0.35022436461601814
1103	13	0.4857002951468061
1103	14	0.5743099127097508
1103	15	0.44878352149067524
1103	16	0.6964631741127931
1103	17	0.6929113081444882
1103	18	0.6495082145245732
1103	19	0.6934238907587797
1103	20	0.5968795370218771
1103	21	0.7023843517369528
1103	22	0.6448978660020543
1103	23	0.7454978829989614
1103	24	0.6839276135590566
1103	25	0.5959925709812097
1104	1	0.15819022247997339
1104	2	0.5929845608837141
1104	3	0.46128982158499454
1104	4	0.39280600053330517
1104	5	0.635864762910586
1104	6	0.5472317487919298
1104	7	0.37648704807637046
1104	8	0.464317230930547
1104	9	0.4009854920081466
1104	10	0.5806056222869387
1104	11	0.47802660628058646
1104	12	0.5358384247673271
1104	13	0.5357471913044307
1104	14	0.46651862856251114
1104	15	0.5374855849216118
1104	16	0.7093363246649886
1104	17	0.6422727750314579
1104	18	0.7566379833358354
1104	19	0.8488365148317243
1104	20	0.8026263350391929
1104	21	0.6644466025040572
1104	22	0.8311330673138997
1104	23	0.7692969065650601
1104	24	0.8707187362095894
1104	25	0.7711186626367947
1105	1	0.03438217746883154
1105	2	0.3100223624498497
1105	3	0.2898979591515207
1105	4	0.1432398538817381
1105	5	0.23284088118662538
1105	6	0.2503847209157115
1105	7	0.4761941590088625
1105	8	0.18458685936940356
1105	9	0.23835594492059187
1105	10	0.3522450094728953
1105	11	0.14079620427359885
1105	12	0.13213407831791812
1105	13	0.3785781072373524
1105	14	0.4251959999896968
1105	15	0.17982906281183375
1105	16	0.2616453378541337
1105	17	0.22431520436663988
1105	18	0.17248413437160082
1105	19	0.02588217142690752
1105	20	0.3136294648724771
1105	21	0.15430661249029498
1105	22	0.20831603468813142
1105	23	0.29617145748878215
1105	24	0.4996310278077516
1105	25	0.5189524404795236
1106	1	0.5294292881341107
1106	2	0.7634281829158205
1106	3	0.5830215335911468
1106	4	0.6648068656683606
1106	5	0.2847641188656027
1106	6	0.17310889961380838
1106	7	0.21046522744770613
1106	8	0.38744036848702024
1106	9	0.5644599705239557
1106	10	0.5518398816352104
1106	11	0.5615962496526876
1106	12	0.44690975034648517
1106	13	0.6329667433567181
1106	14	0.5945466012626994
1106	15	0.6259816918138554
1106	16	0.5652673061126521
1106	17	0.6117407169712834
1106	18	0.6164260252468239
1106	19	0.664130180120587
1106	20	0.6599391294254318
1106	21	0.6159269447130384
1106	22	0.7207923002274867
1106	23	0.5099726731708429
1106	24	0.5189932724010689
1106	25	0.6747161825829693
1107	1	0.05136263959988341
1107	2	0.38065143658468514
1107	3	0.2095381543354442
1107	4	0.15853934914222378
1107	5	0.2859111926449174
1107	6	0.2397101318312096
1107	7	0.0051848447744333725
1107	8	0.33387379681855833
1107	9	0.3347658371559348
1107	10	0.15854878505632541
1107	11	0.23413181485260282
1107	12	0.4306615093900419
1107	13	0.12743536438483005
1107	14	0.11043120800560047
1107	15	0.3184668803714956
1107	16	0.2777538546031266
1107	17	0.26848627945132364
1107	18	-0.091333278467566
1107	19	0.1336588155758065
1107	20	0.22182050780604357
1107	21	0.0728096830264846
1107	22	0.2643722347141946
1107	23	-0.07295957852316093
1107	24	-0.035012321061792306
1107	25	-0.05011027816227909
1108	1	0.1271227309298517
1108	2	0.33411517273862756
1108	3	0.17294886444947366
1108	4	0.05291629684641208
1108	5	0.22336132921378038
1108	6	0.08087003421275085
1108	7	-0.03574467569914219
1108	8	-0.13853841048401705
1108	9	-0.06766933487381976
1108	10	0.048204960628495996
1108	11	-0.1616681231441035
1108	12	-0.10407576267071761
1108	13	0.05001793381897102
1108	14	-0.09090024661812635
1108	15	-0.2186711827455028
1108	16	-0.05385504272265251
1108	17	-0.04887067586369653
1108	18	-0.1618803914208229
1108	19	0.08977752690639562
1108	20	0.0401067778965114
1108	21	0.12557566173084922
1108	22	0.04190382774338275
1108	23	0.05357415817442046
1108	24	-0.038227089012259796
1108	25	-0.2390704594117139
1109	1	-0.17159602717302747
1109	2	0.2732843665652176
1109	3	-0.001419533462659217
1109	4	0.00307018115697133
1109	5	-0.08483709699503095
1109	6	0.04626015630557845
1109	7	-0.03169746196341805
1109	8	0.09160741321980481
1109	9	0.012094032768764862
1109	10	0.032581193675859765
1109	11	-0.009608722672872716
1109	12	0.09327804723424293
1109	13	-0.021114102301567073
1109	14	-0.06328844092948316
1109	15	-0.049240611062472794
1109	16	-0.17750785040671957
1109	17	-0.11529176646942574
1109	18	-0.28729187693991065
1109	19	-0.34695093774531216
1109	20	-0.172517090518312
1109	21	-0.12637184849992167
1109	22	0.027672347410131692
1109	23	0.02624841947336165
1109	24	0.07110968651956995
1109	25	0.19833019220097015
1110	1	-0.18315137921266356
1110	2	0.08533817936244503
1110	3	-0.0015960773945131734
1110	4	0.038616198436209004
1110	5	-0.08542879501663236
1110	6	-0.11206664858027438
1110	7	-0.05623706849112864
1110	8	-0.08028982717786448
1110	9	-0.03360601909101746
1110	10	0.10064577565292865
1110	11	0.13125816320251005
1110	12	0.15679386446714005
1110	13	0.008389423060073398
1110	14	-0.09197407135683022
1110	15	-0.13600091957299298
1110	16	-0.13126299500723349
1110	17	0.20586647206349795
1110	18	-0.06219698457653777
1110	19	0.01001399621622312
1110	20	0.010979637901947115
1110	21	0.05145754385793566
1110	22	-0.14989636679834462
1110	23	-0.015210440273520476
1110	24	-0.08656059561970153
1110	25	-0.1128091781992353
1111	1	-0.03881775776377694
1111	2	0.06554818560646325
1111	3	-0.009567425802561758
1111	4	0.14753069355688098
1111	5	0.06746104032124123
1111	6	0.11593272974452873
1111	7	0.09209654205117346
1111	8	0.010643803541618979
1111	9	-0.04742874895406146
1111	10	0.029308546851915196
1111	11	0.014109198373510878
1111	12	-0.10071463585051532
1111	13	-0.06382578562374874
1111	14	-0.010046551050137964
1111	15	-0.030397935532050724
1111	16	-0.16143060191219405
1111	17	-0.1003071989012696
1111	18	-0.13878709612361462
1111	19	-0.1727775980807209
1111	20	-0.11057422289188423
1111	21	-0.08085157050030745
1111	22	-0.08741528290218525
1111	23	-0.14103968136625156
1111	24	-0.042507546002930496
1111	25	0.021315882127801444
1112	1	-0.29796939006052625
1112	2	-0.02077380754149516
1112	3	-0.08958117201379423
1112	4	-0.07285240966347573
1112	5	0.10463769493583186
1112	6	-0.08041425412078951
1112	7	-0.22345142353589864
1112	8	-0.18654585993954242
1112	9	-0.08978524983228908
1112	10	-0.25459284909953794
1112	11	-0.07353869848250127
1112	12	-0.33795923150913154
1112	13	-0.02336663056806998
1112	14	-0.42699915822186796
1112	15	-0.3976245452476235
1112	16	-0.29046328471441996
1112	17	-0.21797029032411075
1112	18	-0.23088276132700258
1112	19	-0.20344850272362436
1112	20	-0.025026441640982
1112	21	-0.13790756392740633
1112	22	-0.32511940228881997
1112	23	-0.25166508130989484
1112	24	-0.27065120181043767
1112	25	-0.3859551262700265
1113	1	-0.35223040197524824
1113	2	-0.39680434464340664
1113	3	-0.2544310733035893
1113	4	-0.06655915155752723
1113	5	-0.19017506454845545
1113	6	-0.22912432925379989
1113	7	-0.2273895359702962
1113	8	-0.28124073344376654
1113	9	-0.2707955687164372
1113	10	-0.1734033847085641
1113	11	-0.18498213251654505
1113	12	-0.4067140415402548
1113	13	-0.19827544910859984
1113	14	-0.3484775719604727
1113	15	-0.11362743834460076
1113	16	-0.19357027525008164
1113	17	-0.25871173651224666
1113	18	-0.1938207476388456
1113	19	-0.3034945760651766
1113	20	-0.2865943251758125
1113	21	-0.4725776247493627
1113	22	-0.3673588755598445
1113	23	-0.3660929868260894
1113	24	-0.34895529101255107
1113	25	-0.30943863124157645
1114	1	-0.43308658812791734
1114	2	-0.6592409665470775
1114	3	-0.44760350677223215
1114	4	-0.3720152986697869
1114	5	-0.37985388212464954
1114	6	-0.6746404384267919
1114	7	-0.27299924201605563
1114	8	-0.3515485019842117
1114	9	-0.35787819923903186
1114	10	-0.4080362297031357
1114	11	-0.4782531680383073
1114	12	-0.4357566005888671
1114	13	-0.5354669423040548
1114	14	-0.5640707108592338
1114	15	-0.550059145161157
1114	16	-0.7249077094945235
1114	17	-0.5544353104112361
1114	18	-0.6620328471618464
1114	19	-0.6036727604860488
1114	20	-0.3998390334488704
1114	21	-0.36147763626127727
1114	22	-0.4624197635789429
1114	23	-0.3167769447225126
1114	24	-0.42357502243456935
1114	25	-0.4237919869647442
1115	1	-0.47189170164164196
1115	2	-1.0921113823389241
1115	3	-0.5045933817795891
1115	4	-0.4735963476661169
1115	5	-0.4380750425743827
1115	6	-0.7156702051202757
1115	7	-0.4783933514680072
1115	8	-0.6120978220791784
1115	9	-0.5689062980558219
1115	10	-0.7050335611004215
1115	11	-0.8414774680206492
1115	12	-0.5755148565785078
1115	13	-0.6394149045511739
1115	14	-0.6419213083515345
1115	15	-0.89445606057037
1115	16	-0.7566783077717076
1115	17	-0.6530264451560512
1115	18	-0.7491229909916868
1115	19	-0.7188494573053106
1115	20	-0.7686629841582118
1115	21	-0.6639617914326763
1115	22	-0.6727659951587199
1115	23	-0.7241754495201107
1115	24	-0.9677001720529219
1115	25	-0.7793391486310526
1116	1	-0.6435987547010065
1116	2	-1.0087289902997298
1116	3	-0.7456536195402315
1116	4	-0.44866661239322525
1116	5	-0.368565186522126
1116	6	-0.5140864001903509
1116	7	-0.5950422936990094
1116	8	-0.4417295969282051
1116	9	-0.5515917399639818
1116	10	-0.43838366934608075
1116	11	-0.5118599360217146
1116	12	-0.7148574066183855
1116	13	-0.7441948050411327
1116	14	-0.9016824082793704
1116	15	-0.9889241375957027
1116	16	-0.9360764998792632
1116	17	-1.0478857257971654
1116	18	-0.6737735664028293
1116	19	-0.7365010961825931
1116	20	-0.7391660416233634
1116	21	-0.883671996909527
1116	22	-0.8726074247610569
1116	23	-0.7613302977862801
1116	24	-0.8273701177401875
1116	25	-0.8735983305217445
1201	1	0.5092805846807522
1201	2	0.7681412221194606
1201	3	0.5094286861043571
1201	4	0.4779075170140672
1201	5	0.42823222750514844
1201	6	0.43686704143511434
1201	7	0.3964582224089952
1201	8	0.33965248597503717
1201	9	0.4510920216484493
1201	10	0.4099294723934719
1201	11	0.5177809614629396
1201	12	0.6340180212079858
1201	13	0.526889565035475
1201	14	0.5499908681915429
1201	15	0.4724085075936273
1201	16	0.5919970590928614
1201	17	0.498827481134974
1201	18	0.5466398632706628
1201	19	0.5934223025244165
1201	20	0.5993301826391431
1201	21	0.7764437199373191
1201	22	0.7332354610975855
1201	23	0.704921671573203
1201	24	0.7116041855140125
1201	25	0.7245871423961674
1202	1	0.5380045486558664
1202	2	0.7774631248770341
1202	3	0.5386942612954684
1202	4	0.20479309777603305
1202	5	0.30662658236882123
1202	6	0.3006874122066776
1202	7	0.2849513052600372
1202	8	0.3202566145999626
1202	9	0.43712773703948926
1202	10	0.34428000317246443
1202	11	0.38959551378258084
1202	12	0.33849946715518087
1202	13	0.42626439094615165
1202	14	0.4283883179764416
1202	15	0.45735171578897393
1202	16	0.4734615758209131
1202	17	0.500953692022911
1202	18	0.5905815549546105
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2210	15	-0.09004739021890629
2210	16	0.11045877479396182
2210	17	0.02912946411333195
2210	18	0.06537231240014307
2210	19	0.21985049336268503
2210	20	0.15567153717032767
2210	21	-0.022882326634587002
2210	22	-0.0730461304693577
2210	23	-0.04736300174658936
2210	24	0.015816394439575276
2210	25	0.23692279424449936
2211	1	0.09786824540037742
2211	2	-0.4690873521568584
2211	3	-0.045047257567240706
2211	4	-0.1454795912429674
2211	5	-0.0568982704231189
2211	6	0.09542807573620848
2211	7	-0.12969689427737308
2211	8	-0.033789441378978324
2211	9	0.09937839091558232
2211	10	0.07751643541549669
2211	11	-0.0015310232731451379
2211	12	-0.07386738552123973
2211	13	-0.044692784639174477
2211	14	-0.009571148581677846
2211	15	-0.008241063108769708
2211	16	-0.0660955211067602
2211	17	0.09159563195157006
2211	18	0.17907141059307463
2211	19	-0.04992892414186656
2211	20	0.19882186010351433
2211	21	0.19914879319556889
2211	22	0.07919379858387998
2211	23	0.21120237382719864
2211	24	0.20047377301810343
2211	25	0.1450439207419123
2212	1	-0.3431573496348683
2212	2	-1.0296529591325232
2212	3	-0.42342792125990414
2212	4	-0.3128453447834616
2212	5	-0.1639277448139893
2212	6	-0.3465186740863473
2212	7	-0.029132158491208315
2212	8	-0.04125348559441022
2212	9	-0.2573852425596215
2212	10	-0.09957717389700349
2212	11	-0.17385999468019264
2212	12	-0.04156803455742164
2212	13	-0.2033171764079711
2212	14	-0.17395307806145155
2212	15	-0.10243074156297638
2212	16	-0.03966004422019154
2212	17	-0.980953955386461
2212	18	-0.616755079205042
2212	19	-0.3275488344093489
2212	20	-0.21532625748657352
2212	21	-0.21985108125064556
2212	22	-0.12205994560925859
2212	23	-0.06910156881778562
2212	24	-0.06176451781057324
2212	25	-0.0012685134384398111
2213	1	-0.489445264752959
2213	2	-0.6171075009540488
2213	3	-0.4844392907999264
2213	4	-0.3316055853621265
2213	5	-0.4622657738272977
2213	6	-0.5534283172146743
2213	7	-0.732847240615655
2213	8	-0.47620083403384683
2213	9	-0.6164823731994318
2213	10	-0.5258300212774145
2213	11	-0.7565139530385068
2213	12	-0.5374093605375236
2213	13	-0.49143710607268076
2213	14	-0.6505670931675525
2213	15	-0.766807015391052
2213	16	-0.528200586920903
2213	17	-1.4136808466464998
2213	18	-1.1517778582483231
2213	19	-1.0538905999850172
2213	20	-1.0369928242451536
2213	21	-0.7937455982949686
2213	22	-0.8185217007944914
2213	23	-0.7799800606053395
2213	24	-0.6400724744904309
2213	25	-0.8822055513635476
2214	1	-0.7137386370726482
2214	2	-2.071570865247132
2214	3	-1.2124736331918555
2214	4	-0.5491766781267202
2214	5	-0.758999233292954
2214	6	-0.6187877943388571
2214	7	-0.6089580650623958
2214	8	-0.7505601391594041
2214	9	-0.8700298448196548
2214	10	-0.6421154243275211
2214	11	-0.5401683504699903
2214	12	-0.7568151052915653
2214	13	-0.7269175555624159
2214	14	-0.7351115834783002
2214	15	-0.6847851715657995
2214	16	-0.6658281525670446
2214	17	-0.5849484765054598
2214	18	-0.9940981196862282
2214	19	-0.7397924461035856
2214	20	-0.6877854512374739
2214	21	-0.5211349458468533
2214	22	-0.6522061979851799
2214	23	-0.7691799803393167
2214	24	-0.5881862229137766
2214	25	-0.736773548152911
2215	1	-0.8424246479242647
2215	2	-1.9578578641231168
2215	3	-1.1051161820726954
2215	4	-0.7102163239921424
2215	5	-0.881593352327684
2215	6	-0.8326215231594674
2215	7	-0.9848097503932891
2215	8	-0.8183549853356666
2215	9	-0.8965066775909847
2215	10	-0.841912823825183
2215	11	-0.9857853800135814
2215	12	-0.6723057176723941
2215	13	-0.8235552198284068
2215	14	-0.9521161489279706
2215	15	-1.0040891139495471
2215	16	-1.12449626241537
2215	17	-0.8118957309911181
2215	18	-1.0097927202440786
2215	19	-1.10090702640138
2215	20	-0.7526384271058078
2215	21	-0.8613858374278323
2215	22	-1.1153495586557014
2215	23	-1.0872280771435001
2215	24	-0.8416033717973903
2215	25	-1.063168508118494
2216	1	-0.5937584307615076
2216	2	-1.8392793875049556
2216	3	-0.8103158076315751
2216	4	-0.5656833607358109
2216	5	-0.573516247132865
2216	6	-0.6039593209830869
2216	7	-0.6717739188219625
2216	8	-0.6044249464732161
2216	9	-0.7266487513750874
2216	10	-0.7263695896227418
2216	11	-0.6890386100046584
2216	12	-0.629612665562675
2216	13	-0.39301884819777655
2216	14	-0.5875870260933951
2216	15	-0.6357588464074411
2216	16	-0.5970372469162157
2216	17	-0.7969704555608743
2216	18	-0.8088950216604474
2216	19	-0.7236630526995427
2216	20	-0.6601660901437114
2216	21	-0.729742289663676
2216	22	-0.6658434674413201
2216	23	-0.773850753524691
2216	24	-0.6255938318406393
2216	25	-0.5889713417387696
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	6782.0
3	0.0
4	88.0
5	976.0
6	1607.0
7	1100.0
8	925.0
9	663.0
10	682.0
11	609.0
12	549.0
13	469.0
14	464.0
15	464.0
16	493.0
17	521.0
18	635.0
19	889.0
20	1177.0
21	1534.0
22	1877.0
23	2149.0
24	2627.0
25	3014.0
26	3533.0
27	3974.0
28	4436.0
29	5660.0
30	7283.0
31	9356.0
32	12433.0
33	16589.0
34	23250.0
35	33197.0
36	50734.0
37	94714.0
38	252932.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	47.90261850694134	14.95470182124438	13.718250626172646	23.424429045641638
2	11.638654236150625	22.14276856188267	38.054412687296654	28.164164514670055
3	21.434576073379105	28.373496722193348	27.652835143193194	22.539092061234353
4	13.772508680190901	14.910385362120579	33.17358007419029	38.14352588349823
5	17.045218209879923	33.3559552086868	29.616048396218495	19.98277818521478
6	36.777450583294105	30.923892616959865	13.498049604629864	18.800607195116168
7	26.79648064739159	31.87849002771043	21.693857186378654	19.631172138519325
8	26.285405772173693	33.85584340019444	23.325812447218517	16.532938380413352
9	28.1745061919787	20.020974301920518	15.402797789490963	36.40172171660983
10	14.129733042022446	25.692744826982487	29.838636139814632	30.33888599118043
11	32.69365452237478	25.0	18.528313173906067	23.77803230371916
12	26.369381446119334	23.200102134799696	25.62562124403834	24.80489517504263
13	32.062186559679034	19.23078325886751	27.14215373392906	21.56487644752439
14	31.283259595359063	18.736983393454402	23.19737096462401	26.78238604656252
15	32.77830255772478	19.798022531903175	25.512329086870732	21.911345823501307
16	28.265361014615642	21.546176500086617	29.705042989870257	20.483419495427484
17	24.77237707085351	27.99018937425349	27.757870838917913	19.479562715975092
18	26.790849494424542	18.863389771784423	36.07283204318134	18.2729286906097
19	25.26564366275518	31.923010293862887	20.10394157389425	22.707404469487678
20	20.52029234988621	28.163294333897415	28.630849915387756	22.685563400828617
21	21.84558881187234	31.573562960572026	21.845771171496462	24.735077056059172
22	20.729378853557083	29.74905034493952	19.206101134844637	30.31546966665876
23	24.25165806859854	23.950224206251516	19.979977351430307	31.818140373719633
24	19.36142704224633	28.009460610271365	21.00025712144338	31.628855226038926
25	26.802983139964766	27.527590393339967	20.328254936147296	25.341171530547975
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	273.0
1	273.0
2	321.0
3	369.0
4	369.0
5	369.0
6	898.5
7	1428.0
8	1428.0
9	1428.0
10	1358.5
11	1289.0
12	1289.0
13	1289.0
14	1317.5
15	1346.0
16	1346.0
17	1346.0
18	2168.0
19	2990.0
20	2990.0
21	2990.0
22	4514.5
23	6039.0
24	6039.0
25	6039.0
26	8772.0
27	11505.0
28	11505.0
29	11505.0
30	13712.5
31	15920.0
32	15920.0
33	15920.0
34	18652.5
35	21385.0
36	21385.0
37	21385.0
38	23525.5
39	25666.0
40	25666.0
41	25666.0
42	31082.5
43	36499.0
44	36499.0
45	36499.0
46	64788.5
47	93078.0
48	93078.0
49	93078.0
50	142288.0
51	191498.0
52	191498.0
53	191498.0
54	122995.0
55	54492.0
56	54492.0
57	54492.0
58	41334.0
59	28176.0
60	28176.0
61	28176.0
62	24705.0
63	21234.0
64	21234.0
65	21234.0
66	17921.5
67	14609.0
68	14609.0
69	14609.0
70	11468.0
71	8327.0
72	8327.0
73	8327.0
74	6856.5
75	5386.0
76	5386.0
77	5386.0
78	4620.5
79	3855.0
80	3855.0
81	3855.0
82	2830.0
83	1805.0
84	1805.0
85	1805.0
86	1333.0
87	861.0
88	861.0
89	861.0
90	590.0
91	319.0
92	319.0
93	319.0
94	175.0
95	31.0
96	31.0
97	31.0
98	18.0
99	5.0
100	5.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.18454188207190206
2	0.010394157389425313
3	0.0
4	0.15937707997118813
5	0.044311934133865805
6	0.05306490877759238
7	0.03938838589676961
8	0.02443538754707003
9	0.01732359564904219
10	0.010758864666247253
11	0.016229473818576364
12	0.016411827456987337
13	0.006382377344383964
14	0.008570621005315608
15	0.0012764754688767926
16	0.0
17	0.0
18	0.0
19	0.0
20	1.823536384109704E-4
21	0.0032823654913974668
22	0.005105901875507171
23	0.0010941218304658224
24	0.0010941218304658224
25	0.11670632858302105
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	548385.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	35.915534435069056
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.06272430743124	31.987353417446084
2	6.235357859950258	4.47892419868044
3	1.4744809630904152	1.5887031541118277
4	0.7252698312602242	1.041938143973732
5	0.4349651941788958	0.7811003704794315
6	0.2846156243193603	0.6133273353600398
7	0.20533569677031493	0.5162318901672203
8	0.15599436018323484	0.4482096651870029
9	0.11819943540054426	0.38206763031005714
>10	0.9873206125020273	7.188594888852038
>50	0.14996190272143256	3.8049805534448846
>100	0.12853877376122785	9.187095318955583
>500	0.012241787977259798	3.207578454995978
>1k	0.018872756464942186	12.101743398171187
>5k	0.0035705214933674408	7.335899917291619
>10k+	0.0025503724952624577	15.336251662572867
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTACGGAAGCAGTGGTATCAACGCA	26877	4.901118739571651	No Hit
CTTCCGTACTCTGCGTTGATACCAC	15977	2.9134640808920738	No Hit
GAGTACGGAAGCAGTGGTATCAACG	15094	2.7524458181751874	No Hit
TCCGTACTCTGCGTTGATACCACTG	13306	2.426397512696372	No Hit
GCTTCCGTACTCTGCGTTGATACCA	12461	2.272308688239102	No Hit
CCCATGTACTCTGCGTTGATACCAC	6450	1.176180967750759	No Hit
GCGTTGATACCACTGCTTCCGTACT	6419	1.170528004960019	No Hit
GTATCAACGCAGAGTACGGAAGCAG	6383	1.163963273977224	No Hit
GTACATGGGAAGCAGTGGTATCAAC	5306	0.9675684054086089	No Hit
TCCATGTACTCTGCGTTGATACCAC	5268	0.9606389671489921	No Hit
TATCAACGCAGAGTACGGAAGCAGT	5203	0.9487859806522789	No Hit
GTACATGGAAGCAGTGGTATCAACG	5015	0.9145034966310165	No Hit
ACTCTGCGTTGATACCACTGCTTCC	3988	0.7272263099829499	No Hit
CAGTGGTATCAACGCAGAGTACGGA	3588	0.6542848546185618	No Hit
ACGCAGAGTACGGAAGCAGTGGTAT	3411	0.6220082606198201	No Hit
GGTATCAACGCAGAGTACGGAAGCA	3181	0.5800669237852968	No Hit
CATGTACTCTGCGTTGATACCACTG	2894	0.5277314295613483	No Hit
ATACCACTGCTTCCGTACTCTGCGT	2849	0.5195255158328547	No Hit
AAGCAGTGGTATCAACGCAGAGTAC	2617	0.4772194717215095	No Hit
GTGGTATCAACGCAGAGTACGGAAG	2324	0.4237898556670952	No Hit
GAAGCAGTGGTATCAACGCAGAGTA	2285	0.4166780637690674	No Hit
GTACGGGAAGCAGTGGTATCAACGC	2120	0.38658971343125725	No Hit
GAGTACATGGGAAGCAGTGGTATCA	1994	0.36361315499147495	No Hit
GAGTACATGGAAGCAGTGGTATCAA	1944	0.35449547307092644	No Hit
ATCAACGCAGAGTACGGAAGCAGTG	1764	0.3216718181569518	No Hit
CGGAAGCAGTGGTATCAACGCAGAG	1648	0.30051879610127924	No Hit
GATACCACTGCTTCCGTACTCTGCG	1625	0.2963246624178269	No Hit
CCCGTACTCTGCGTTGATACCACTG	1519	0.27699517674626406	No Hit
TTCCCGTACTCTGCGTTGATACCAC	1500	0.27353045761645556	No Hit
CCACTGCTTCCGTACTCTGCGTTGA	1484	0.2706127994018801	No Hit
GTATCAACGCAGAGTACATGGGAAG	1479	0.26970103120982525	No Hit
GTTGATACCACTGCTTCCGTACTCT	1464	0.26696572663366064	No Hit
GTACTCTGCGTTGATACCACTGCTT	1422	0.2593068738203999	No Hit
GCTTCCCATGTACTCTGCGTTGATA	1369	0.24964213098461846	No Hit
GCGTTGATACCACTGCTTCCCATGT	1359	0.24781859460050876	No Hit
CATGGAAGCAGTGGTATCAACGCAG	1337	0.2438068145554674	No Hit
GTATCAACGCAGAGTACTTTTTTTT	1319	0.24052444906406995	No Hit
GAGTACGGGAAGCAGTGGTATCAAC	1311	0.23906561995678222	No Hit
GCTTCCATGTACTCTGCGTTGATAC	1302	0.23742443721108344	No Hit
CTGCTTCCGTACTCTGCGTTGATAC	1269	0.23140676714352143	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	1153	0.2102537450878489	No Hit
CAGTGGTATCAACGCAGAGTACATG	1128	0.2056949041275746	No Hit
TATCAACGCAGAGTACATGGGAAGC	1110	0.20241253863617714	No Hit
CTTCCCGTACTCTGCGTTGATACCA	1097	0.20004194133683453	No Hit
GCAGAGTACGGAAGCAGTGGTATCA	1060	0.19329485671562863	No Hit
AAAAAGTACTCTGCGTTGATACCAC	1050	0.1914713203315189	No Hit
GCGTTGATACCACTGCTTCCATGTA	1038	0.18928307667058727	No Hit
GCAGTGGTATCAACGCAGAGTACGG	1033	0.18837130847853242	No Hit
TATCAACGCAGAGTACTTTTTTTTT	1024	0.18673012573283368	No Hit
ACGCAGAGTACATGGGAAGCAGTGG	957	0.17451243195929866	No Hit
GTATCAACGCAGAGTACGGGAAGCA	940	0.17141242010631216	No Hit
GTATCAACGCAGAGTACATGGAAGC	886	0.16156532363211978	No Hit
CCCCATGTACTCTGCGTTGATACCA	869	0.15846531177913328	No Hit
GCCCATGTACTCTGCGTTGATACCA	866	0.15791825086390038	No Hit
CACTGCTTCCGTACTCTGCGTTGAT	844	0.15390647081885903	No Hit
GCTTCCCGTACTCTGCGTTGATACC	767	0.13986524066121428	No Hit
AAAGTACTCTGCGTTGATACCACTG	759	0.13840641155392655	No Hit
TGATACCACTGCTTCCGTACTCTGC	748	0.13640052153140586	No Hit
GCGTTGATACCACTGCTTCCCGTAC	736	0.1342122778704742	No Hit
TATCAACGCAGAGTACATGGAAGCA	735	0.13402992423206325	No Hit
CTGCGTTGATACCACTGCTTCCGTA	719	0.1311122660174877	No Hit
TATCAACGCAGAGTACGGGAAGCAG	714	0.13020049782543286	No Hit
TACGGAAGCAGTGGTATCAACGCAG	713	0.1300181441870219	No Hit
TCAACGCAGAGTACGGAAGCAGTGG	708	0.12910637599496702	No Hit
ACGCAGAGTACATGGAAGCAGTGGT	697	0.12710048597244636	No Hit
GGTATCAACGCAGAGTACATGGGAA	694	0.12655342505721345	No Hit
TACCACTGCTTCCGTACTCTGCGTT	664	0.12108281590488434	No Hit
CCATGTACTCTGCGTTGATACCACT	658	0.11998869407441853	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	616	0.11232984126115778	No Hit
GGGAAGCAGTGGTATCAACGCAGAG	604	0.11014159760022611	No Hit
GGTATCAACGCAGAGTACTTTTTTT	568	0.10357686661743118	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	5.470609152329112E-4	0.0
11	0.0	0.0	0.0	5.470609152329112E-4	0.0
12	0.0	0.0	0.0	0.0010941218304658224	5.470609152329112E-4
13	0.0	0.0	0.0	0.0020058900225206743	5.470609152329112E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTCCGT	25	0.0059206286	19.058765	1
ACCCGCA	25	0.0060437773	18.99097	7
TTAGCTC	55	5.80967E-7	17.29607	4
AACGCCA	35	0.0021786636	16.273521	15
CGCATCG	75	5.8687874E-8	15.190006	13
GCATCGC	75	5.8687874E-8	15.190006	14
GCTCGCT	45	6.6975516E-4	14.793694	19
CGCTCGC	45	6.786216E-4	14.768062	18
CGCCAGT	90	2.2955646E-9	14.768062	18
GGTCGCT	45	6.79091E-4	14.766714	15
CATCGCC	85	1.7254933E-8	14.518533	15
GTATTAG	60	2.4887124E-5	14.294073	1
AAGATCC	40	0.005226287	14.269258	4
GATCCAA	40	0.005283593	14.245827	6
AGCTCTA	60	2.5693693E-5	14.245826	6
GTCGTAG	60	2.5782265E-5	14.240629	11
CGTCGTA	60	2.5782265E-5	14.240629	10
TCGCCAG	95	4.9258233E-9	13.989518	17
TTACCGC	90	3.657442E-8	13.711948	16
GCCGCAT	85	2.7098758E-7	13.4029455	11
>>END_MODULE