FastQCFastQC Report
Thu 2 Feb 2017
SRR4050223_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4050223_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10768456
Sequences flagged as poor quality0
Sequence length25
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACAAAGGGCAGGGACTTAATCAA222410.20653843039336373No Hit
GTATCTGATCGTCTTCGAACCTCCG186580.17326532234519043No Hit
GAATAGGACCGCGGTTCTATTTTGT168510.15648482939429756No Hit
GTCCTATTCCATTATTCCTAGCTGC167510.15555619115683808No Hit
CTATTGGAGCTGGAATTACCGCGGC161900.15034653064469036No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG158060.14678055981284596No Hit
GATTAAGAGGGACGGCCGGGGGCAT150210.13949074964878902No Hit
GAACTACGACGGTATCTGATCGTCT143720.13346388748767699No Hit
GTCTTGCGCCGGTCCAAGAATTTCA142450.13228451692610343No Hit
ATACAGGACTCTTTCGAGGCCCTGT142040.13190377524874503No Hit
CTCTTAATCATGGCCTCAGTTCCGA138300.12843066824064658No Hit
CTTTAATATACGCTATTGGAGCTGG128250.11909785395417877No Hit
ATCAGATACCGTCGTAGTTCCGACC124950.11603334777056246No Hit
ATATTAAAGTTGCTGCAGTTAAAAA123520.1147053950909954No Hit
GCTTTGAACACTCTAATTTTTTCAA121150.11250452246821642No Hit
GTGTAGCGCGCGTGCAGCCCCGGAC120790.11217021270273102No Hit
CTTATGACCCGCACTTACTGGGAAT118990.11049866387530395No Hit
GGGTAGGCACACGCTGAGCCAGTCA117390.10901284269536878No Hit
GTCCTGTATTGTTATTTTTCGTCAC116760.10842780060576929No Hit
GACTTAATCAACGCAAGCTTATGAC115630.10737843939744007No Hit
TCGTAGTTCCGACCATAAACGATGC112520.10449037447894109No Hit
GAATAACGCCGCCGCATCGCCAGTC111320.1033760085939897No Hit
CTCCCGACCCGGGGAGGTAGTGACG110370.1024938022684032No Hit
GTGCATGGCCGTTCTTAGTTGGTGG110120.10226164270903833No Hit
GTATCAACGCAGAGTACGGGCGGTG109610.10178803720793399No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA55950.016.0552671
GTATCAA189850.013.3820711
CTCGACG6650.012.44040513
ACGCTTA6900.012.12668317
CGCTTAC7350.011.513618
ACTCTAA26100.011.40511410
GCTCGTA14700.011.3865249
TCGACGC7350.011.38466114
CGTCGTA22000.011.36917210
GACGCTT7400.011.30773616
CGACCAT22500.011.07425610
CGCCAGT20100.011.06950818
GGTAGCG7500.011.03204111
ACCGTCC7500.010.9051848
CGAACGA12850.010.87774616
CGGTTCT30350.010.87129512
AACCGCG19350.010.7632327
CGGTCCA28150.010.70968310
CGAGCCG28350.010.69947115
GGTTCTA31100.010.67092313
Produced by FastQC (version 0.11.5)