##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR4050223_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 10768456 Sequences flagged as poor quality 0 Sequence length 25 %GC 52 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.692656588836876 31.0 31.0 34.0 27.0 34.0 2 30.814109469361252 31.0 31.0 34.0 27.0 34.0 3 30.918299707961847 33.0 31.0 34.0 27.0 34.0 4 34.45230504726026 37.0 35.0 37.0 32.0 37.0 5 34.45422509968003 37.0 35.0 37.0 31.0 37.0 6 34.48614759627564 37.0 35.0 37.0 31.0 37.0 7 34.41476902538302 37.0 35.0 37.0 31.0 37.0 8 34.39249545152991 37.0 35.0 37.0 31.0 37.0 9 35.9789477711568 39.0 37.0 39.0 31.0 39.0 10 35.87104214383195 39.0 37.0 39.0 31.0 39.0 11 35.76043250768727 39.0 37.0 39.0 31.0 39.0 12 35.68696895822391 39.0 37.0 39.0 30.0 39.0 13 35.690111284291824 39.0 37.0 39.0 30.0 39.0 14 36.883425627592295 40.0 38.0 41.0 31.0 41.0 15 36.83308869906698 40.0 38.0 41.0 31.0 41.0 16 36.83301403655269 40.0 38.0 41.0 31.0 41.0 17 36.79547300002897 40.0 38.0 41.0 31.0 41.0 18 36.75550506033548 40.0 37.0 41.0 30.0 41.0 19 36.76427428407564 40.0 38.0 41.0 30.0 41.0 20 36.71858797584352 40.0 37.0 41.0 30.0 41.0 21 36.66552893005274 40.0 37.0 41.0 30.0 41.0 22 36.584557247575695 40.0 37.0 41.0 30.0 41.0 23 36.50001690121592 40.0 37.0 41.0 30.0 41.0 24 36.42950261393091 40.0 37.0 41.0 30.0 41.0 25 36.19238811952243 40.0 37.0 41.0 30.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.5114248305663018 1101 2 0.5285180335102098 1101 3 0.5364494036515524 1101 4 0.5363334522683942 1101 5 0.5250877759296273 1101 6 0.5501579596699173 1101 7 0.5760873378396383 1101 8 0.5556545354355364 1101 9 0.5709287973199011 1101 10 0.6064629412063383 1101 11 0.4750604850977709 1101 12 0.5611849280238488 1101 13 0.4850366375458961 1101 14 0.6066023499140556 1101 15 0.6625228924777886 1101 16 0.6865761388774061 1101 17 0.6755272472048759 1101 18 0.6901525264990838 1101 19 0.6998413891598432 1101 20 0.6756246131025492 1101 21 0.7072815831589878 1101 22 0.7596874291419127 1101 23 0.7416134644590358 1101 24 0.7685759925922753 1101 25 0.800832944302627 1102 1 0.4701021059523889 1102 2 0.48419276552915136 1102 3 0.46144688201341566 1102 4 0.4625468833178914 1102 5 0.4006473146898202 1102 6 0.4214883725975511 1102 7 0.4068880176357226 1102 8 0.3905340990472794 1102 9 0.5140765374157255 1102 10 0.5753728196030252 1102 11 0.4820693648855112 1102 12 0.472142498645475 1102 13 0.4834924862776546 1102 14 0.6068000235419149 1102 15 0.5757982408762956 1102 16 0.6483624398367951 1102 17 0.5236332052322084 1102 18 0.6174589133122055 1102 19 0.6476263614305466 1102 20 0.623890842694216 1102 21 0.6589424339623804 1102 22 0.6760443232938727 1102 23 0.6853007747577422 1102 24 0.6056693499401931 1102 25 0.6938783501529073 1103 1 0.5519610161505994 1103 2 0.4864207541305987 1103 3 0.4830575155386363 1103 4 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-0.9407688937853678 2215 20 -0.9858996522961121 2215 21 -0.8592312417534131 2215 22 -0.942508068492387 2215 23 -0.7835626042679138 2215 24 -0.9237810749349009 2215 25 -0.8675210226037535 2216 1 -0.5101792023327505 2216 2 -0.6253523462886328 2216 3 -0.6189741939332905 2216 4 -0.5345594717415665 2216 5 -0.5738745802101448 2216 6 -0.5209217671031965 2216 7 -0.6044523461414428 2216 8 -0.5813044125103488 2216 9 -0.526036418764221 2216 10 -0.5876496172789913 2216 11 -0.4315953560214254 2216 12 -0.505290922638693 2216 13 -0.5191767128062992 2216 14 -0.5739208814795589 2216 15 -0.563983450313529 2216 16 -0.7377365595952199 2216 17 -0.8058760998380947 2216 18 -0.6820044493809974 2216 19 -0.6740487626528946 2216 20 -0.6078305544043445 2216 21 -0.6981381032164293 2216 22 -0.6583325159855207 2216 23 -0.812719994033003 2216 24 -0.9581955897164036 2216 25 -0.8323907136168742 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 262686.0 3 16.0 4 2225.0 5 12686.0 6 17097.0 7 17138.0 8 14605.0 9 14151.0 10 14178.0 11 16735.0 12 28735.0 13 26011.0 14 11368.0 15 11635.0 16 12337.0 17 13110.0 18 14509.0 19 17085.0 20 21413.0 21 27149.0 22 33452.0 23 40060.0 24 47311.0 25 54589.0 26 63321.0 27 72800.0 28 84255.0 29 102838.0 30 131081.0 31 170045.0 32 220162.0 33 295782.0 34 411914.0 35 584788.0 36 879187.0 37 1634448.0 38 5387554.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.65512440823854 16.91489664436171 12.238379267829288 26.19159967957046 2 17.603533536957986 19.909630166212857 36.633007024176194 25.853829272652963 3 18.76288055606978 23.54870308370536 27.77157193715563 29.916844423069232 4 13.382021417062544 15.780333821535397 35.72383934309421 35.11380541830785 5 16.605967661475386 34.35015098617819 32.001487440394925 17.042393911951493 6 34.175171721129125 35.35268670758212 15.637922760711497 14.834218810577257 7 30.704079093565323 29.82965975249625 20.458130767448203 19.008130386490226 8 27.882121484712595 31.198856646827473 19.253098091447253 21.66592377701268 9 27.821639210693068 14.646080037437894 18.1142333413776 39.41804741049143 10 17.231103168110074 24.871342340329758 29.232358428252237 28.66519606330793 11 37.57531932475803 21.255740279693295 21.516929260039685 19.652011135508992 12 26.094767668992503 23.01675213074363 26.957647441775507 23.930832758488364 13 29.965778395667858 19.306517061818006 24.06402865161943 26.663675890894705 14 24.85068886119133 19.701956373448873 23.74700777103142 31.700346994328378 15 25.833865952680966 25.88489831808205 21.927071487285424 26.354164241951562 16 26.521342126674636 25.375277183680122 22.271923138446137 25.831457551199104 17 26.09091850703485 24.467529986026403 23.439122486425568 26.002429020513183 18 27.648266282183435 23.064057331430362 24.12627888135126 25.161397505034937 19 28.111583448914413 23.84673519138095 23.35663901570918 24.685042343995462 20 27.054975516834368 23.55711998504131 23.17174677745445 26.21615772066987 21 27.59218549950343 23.695087303913944 23.20081508082754 25.511912115755088 22 26.6470020955267 23.422998368151585 23.91833952318915 26.011660013132566 23 25.301915524595163 22.825191275790075 24.340077960961196 27.532815238653562 24 25.878913543981362 24.487616121789983 23.54042258670416 26.09304774752449 25 25.81381332315356 23.73035886508727 23.76671965309004 26.689108158669132 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 4068.0 1 4068.0 2 4483.5 3 4899.0 4 4899.0 5 4899.0 6 8411.5 7 11924.0 8 11924.0 9 11924.0 10 16700.5 11 21477.0 12 21477.0 13 21477.0 14 29824.5 15 38172.0 16 38172.0 17 38172.0 18 60585.5 19 82999.0 20 82999.0 21 82999.0 22 123208.0 23 163417.0 24 163417.0 25 163417.0 26 230139.0 27 296861.0 28 296861.0 29 296861.0 30 352788.5 31 408716.0 32 408716.0 33 408716.0 34 529621.0 35 650526.0 36 650526.0 37 650526.0 38 729135.5 39 807745.0 40 807745.0 41 807745.0 42 923916.5 43 1040088.0 44 1040088.0 45 1040088.0 46 1127538.0 47 1214988.0 48 1214988.0 49 1214988.0 50 1259724.0 51 1304460.0 52 1304460.0 53 1304460.0 54 1231078.5 55 1157697.0 56 1157697.0 57 1157697.0 58 1105775.5 59 1053854.0 60 1053854.0 61 1053854.0 62 958420.0 63 862986.0 64 862986.0 65 862986.0 66 745197.0 67 627408.0 68 627408.0 69 627408.0 70 505641.5 71 383875.0 72 383875.0 73 383875.0 74 324132.5 75 264390.0 76 264390.0 77 264390.0 78 228694.0 79 192998.0 80 192998.0 81 192998.0 82 148138.5 83 103279.0 84 103279.0 85 103279.0 86 75591.5 87 47904.0 88 47904.0 89 47904.0 90 34799.0 91 21694.0 92 21694.0 93 21694.0 94 11704.5 95 1715.0 96 1715.0 97 1715.0 98 1015.5 99 316.0 100 316.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1429174247450145 2 0.16067298784523984 3 0.17389679634666289 4 0.1936860771869245 5 0.18377750719323177 6 0.06875637510150016 7 0.05677694183827282 8 0.05968357952152101 9 0.06655550247872119 10 0.06816204662952609 11 0.7040470797299074 12 0.6627598236924588 13 0.6604475144811847 14 0.6608468289232923 15 0.6623326501032274 16 0.6688888360596914 17 0.6606332421286766 18 0.6704210891514994 19 0.6665765268484173 20 0.671693323536819 21 0.660373223422188 22 0.6628991194280777 23 0.6478923255107324 24 0.6589524069188749 25 0.6511425593418407 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1.0768456E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 37.6334503220346 #Duplication Level Percentage of deduplicated Percentage of total 1 79.9375689359651 30.083265294158583 2 12.418351816692658 9.346908523501025 3 3.14733140530422 3.5533482026548713 4 1.2608843137719428 1.8980570873667637 5 0.6773189126794172 1.274492382624767 6 0.4499912779980148 1.016083464353229 7 0.3072435960001226 0.8093845624783716 8 0.21733058051514087 0.6543119684220394 9 0.17968384727852027 0.608591082620542 >10 1.0995766330357715 8.131685510904116 >50 0.1378258871778294 3.6322522656480194 >100 0.12482503791409495 9.867705852323768 >500 0.02084877924434242 5.487536852077669 >1k 0.01819123948407235 14.229714537343192 >5k 0.0023328464938426855 5.920969617227105 >10k+ 6.948904449744169E-4 3.485692796295937 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTACAAAGGGCAGGGACTTAATCAA 22241 0.20653843039336373 No Hit GTATCTGATCGTCTTCGAACCTCCG 18658 0.17326532234519043 No Hit GAATAGGACCGCGGTTCTATTTTGT 16851 0.15648482939429756 No Hit GTCCTATTCCATTATTCCTAGCTGC 16751 0.15555619115683808 No Hit CTATTGGAGCTGGAATTACCGCGGC 16190 0.15034653064469036 No Hit GTTCAAAGCAGGCCCGAGCCGCCTG 15806 0.14678055981284596 No Hit GATTAAGAGGGACGGCCGGGGGCAT 15021 0.13949074964878902 No Hit GAACTACGACGGTATCTGATCGTCT 14372 0.13346388748767699 No Hit GTCTTGCGCCGGTCCAAGAATTTCA 14245 0.13228451692610343 No Hit ATACAGGACTCTTTCGAGGCCCTGT 14204 0.13190377524874503 No Hit CTCTTAATCATGGCCTCAGTTCCGA 13830 0.12843066824064658 No Hit CTTTAATATACGCTATTGGAGCTGG 12825 0.11909785395417877 No Hit ATCAGATACCGTCGTAGTTCCGACC 12495 0.11603334777056246 No Hit ATATTAAAGTTGCTGCAGTTAAAAA 12352 0.1147053950909954 No Hit GCTTTGAACACTCTAATTTTTTCAA 12115 0.11250452246821642 No Hit GTGTAGCGCGCGTGCAGCCCCGGAC 12079 0.11217021270273102 No Hit CTTATGACCCGCACTTACTGGGAAT 11899 0.11049866387530395 No Hit GGGTAGGCACACGCTGAGCCAGTCA 11739 0.10901284269536878 No Hit GTCCTGTATTGTTATTTTTCGTCAC 11676 0.10842780060576929 No Hit GACTTAATCAACGCAAGCTTATGAC 11563 0.10737843939744007 No Hit TCGTAGTTCCGACCATAAACGATGC 11252 0.10449037447894109 No Hit GAATAACGCCGCCGCATCGCCAGTC 11132 0.1033760085939897 No Hit CTCCCGACCCGGGGAGGTAGTGACG 11037 0.1024938022684032 No Hit GTGCATGGCCGTTCTTAGTTGGTGG 11012 0.10226164270903833 No Hit GTATCAACGCAGAGTACGGGCGGTG 10961 0.10178803720793399 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 1.857276474918967E-5 2 0.0 0.0 0.0 0.0 1.857276474918967E-5 3 0.0 0.0 0.0 0.0 1.857276474918967E-5 4 0.0 0.0 0.0 9.286382374594834E-6 2.7859147123784505E-5 5 0.0 0.0 0.0 1.857276474918967E-5 2.7859147123784505E-5 6 9.286382374594834E-6 0.0 0.0 1.857276474918967E-5 2.7859147123784505E-5 7 9.286382374594834E-6 0.0 0.0 1.857276474918967E-5 2.7859147123784505E-5 8 9.286382374594834E-6 9.286382374594834E-6 0.0 1.857276474918967E-5 2.7859147123784505E-5 9 9.286382374594834E-6 9.286382374594834E-6 0.0 6.500467662216385E-5 2.7859147123784505E-5 10 9.286382374594834E-6 9.286382374594834E-6 0.0 8.357744137135352E-5 3.714552949837934E-5 11 9.286382374594834E-6 9.286382374594834E-6 0.0 8.357744137135352E-5 3.714552949837934E-5 12 9.286382374594834E-6 9.286382374594834E-6 0.0 9.286382374594835E-5 8.357744137135352E-5 13 9.286382374594834E-6 9.286382374594834E-6 0.0 1.1143658849513802E-4 9.286382374594835E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5595 0.0 16.055267 1 GTATCAA 18985 0.0 13.382071 1 CTCGACG 665 0.0 12.440405 13 ACGCTTA 690 0.0 12.126683 17 CGCTTAC 735 0.0 11.5136 18 ACTCTAA 2610 0.0 11.405114 10 GCTCGTA 1470 0.0 11.386524 9 TCGACGC 735 0.0 11.384661 14 CGTCGTA 2200 0.0 11.369172 10 GACGCTT 740 0.0 11.307736 16 CGACCAT 2250 0.0 11.074256 10 CGCCAGT 2010 0.0 11.069508 18 GGTAGCG 750 0.0 11.032041 11 ACCGTCC 750 0.0 10.905184 8 CGAACGA 1285 0.0 10.877746 16 CGGTTCT 3035 0.0 10.871295 12 AACCGCG 1935 0.0 10.763232 7 CGGTCCA 2815 0.0 10.709683 10 CGAGCCG 2835 0.0 10.699471 15 GGTTCTA 3110 0.0 10.670923 13 >>END_MODULE