FastQCFastQC Report
Thu 2 Feb 2017
SRR4050223_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4050223_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10768456
Sequences flagged as poor quality0
Sequence length25
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACAAAGGGCAGGGACTTAATCAA218350.20276815914927823No Hit
GAATAGGACCGCGGTTCTATTTTGT172670.16034796446212904No Hit
GTATCTGATCGTCTTCGAACCTCCG168420.1564012519529262No Hit
GATTAAGAGGGACGGCCGGGGGCAT167220.15528688606797483No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG161970.15041153532131254No Hit
CTATTGGAGCTGGAATTACCGCGGC160290.1488514230823806No Hit
GTCCTATTCCATTATTCCTAGCTGC151980.1411344393290923No Hit
ATACAGGACTCTTTCGAGGCCCTGT150170.13945360411929064No Hit
GAACTACGACGGTATCTGATCGTCT141580.13147660165951366No Hit
CTCTTAATCATGGCCTCAGTTCCGA137460.1276506121211806No Hit
ATCAGATACCGTCGTAGTTCCGACC137120.1273348751204444No Hit
CTTTAATATACGCTATTGGAGCTGG132430.1229795617867594No Hit
ATATTAAAGTTGCTGCAGTTAAAAA131540.12215307375542045No Hit
CTTATGACCCGCACTTACTGGGAAT126750.11770489659798955No Hit
GTCTTGCGCCGGTCCAAGAATTTCA124930.11601477500581328No Hit
TCGTAGTTCCGACCATAAACGATGC123820.11498398656223324No Hit
GGGTAGGCACACGCTGAGCCAGTCA120080.11151087955413477No Hit
GCTTTGAACACTCTAATTTTTTCAA119560.11102798767065586No Hit
GACTTAATCAACGCAAGCTTATGAC116010.10773132192767468No Hit
GAATAACGCCGCCGCATCGCCAGTC115050.10683982921971358No Hit
GCGCAAGACGGACCAGAGCGAAAGC112790.10474110680305515No Hit
GTGTAGCGCGCGTGCAGCCCCGGAC112700.1046575293616838No Hit
CTCCCGACCCGGGGAGGTAGTGACG109140.10135157723632802No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA61100.016.0179841
GTATCAA191650.013.1095121
CGGTAGC9250.012.00959910
GGTAGCG8750.011.93627811
CGTCGTA24350.011.73685810
ACGCTTA5700.011.489449517
CGAACGA12350.011.29742316
GCTCGTA15950.011.1322119
CGCTTAC6000.011.07316518
CTCGACG5950.011.00705713
ACCGTCC8750.010.9600818
TCGACGC6700.010.90804514
ACTCTAA25200.010.88884110
AAGCTCG17050.010.8047857
CCGTCCA8800.010.7899099
TACCGTC27850.010.7063947
GTCGTAG26750.010.64833711
ATCGCCA20200.010.619060516
TCCATTA30000.010.6028848
CGAGCCG30450.010.59791915
Produced by FastQC (version 0.11.5)