FastQCFastQC Report
Thu 2 Feb 2017
SRR4050222_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4050222_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences485932
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACGGAAGCAGTGGTATCAACGCA296986.111554703127187No Hit
CTTCCGTACTCTGCGTTGATACCAC170773.51427771786999No Hit
GAGTACGGAAGCAGTGGTATCAACG161603.32556818649523No Hit
TCCGTACTCTGCGTTGATACCACTG145662.9975387502778164No Hit
GCTTCCGTACTCTGCGTTGATACCA133972.756970111044344No Hit
GTACATGGGAAGCAGTGGTATCAAC101842.0957664858457563No Hit
CCCATGTACTCTGCGTTGATACCAC86861.787492900241186No Hit
GCGTTGATACCACTGCTTCCGTACT74221.5273742005054205No Hit
GTACATGGAAGCAGTGGTATCAACG66751.3736489879242364No Hit
GTATCAACGCAGAGTACGGAAGCAG65501.3479252241054305No Hit
TCCATGTACTCTGCGTTGATACCAC50691.0431500703802177No Hit
ACTCTGCGTTGATACCACTGCTTCC46960.9663903591449009No Hit
TATCAACGCAGAGTACGGAAGCAGT44900.9239975963715088No Hit
GAGTACATGGGAAGCAGTGGTATCA37510.7719187046747281No Hit
CATGTACTCTGCGTTGATACCACTG36060.7420791386449133No Hit
CAGTGGTATCAACGCAGAGTACGGA34190.7035963879719797No Hit
GGTATCAACGCAGAGTACGGAAGCA32280.6642904768568442No Hit
ACGCAGAGTACGGAAGCAGTGGTAT29730.6118139986664801No Hit
ATACCACTGCTTCCGTACTCTGCGT29660.610373467892627No Hit
GAGTACATGGAAGCAGTGGTATCAA25120.5169447577027239No Hit
GTATCAACGCAGAGTACATGGGAAG24920.5128289554917149No Hit
GTACGGGAAGCAGTGGTATCAACGC24450.5031568202958439No Hit
AAGCAGTGGTATCAACGCAGAGTAC23900.49183836421556926No Hit
GCGTTGATACCACTGCTTCCCATGT22900.47125935316052453No Hit
GTGGTATCAACGCAGAGTACGGAAG22080.4543845640953878No Hit
GTACTCTGCGTTGATACCACTGCTT21320.4387445156935539No Hit
GAAGCAGTGGTATCAACGCAGAGTA21050.43318818270869175No Hit
GCTTCCCATGTACTCTGCGTTGATA19470.40067334524172105No Hit
GATACCACTGCTTCCGTACTCTGCG16890.3475794967197056No Hit
CCCGTACTCTGCGTTGATACCACTG16850.34675633627750385No Hit
TATCAACGCAGAGTACATGGGAAGC16620.34202316373484354No Hit
GTTGATACCACTGCTTCCGTACTCT16580.3412000032926418No Hit
CCACTGCTTCCGTACTCTGCGTTGA16100.3313220779862203No Hit
TTCCCGTACTCTGCGTTGATACCAC15870.32658890544356No Hit
CGGAAGCAGTGGTATCAACGCAGAG14930.3072446350518179No Hit
ACGCAGAGTACATGGGAAGCAGTGG14750.30354041306190993No Hit
CAGTGGTATCAACGCAGAGTACATG14540.2992188207403505No Hit
ATCAACGCAGAGTACGGAAGCAGTG14090.28995826576558037No Hit
GCTTCCATGTACTCTGCGTTGATAC13790.28378456244906697No Hit
CTGCTTCCGTACTCTGCGTTGATAC13480.2774050690220031No Hit
CATGGAAGCAGTGGTATCAACGCAG12950.26649819316282936No Hit
GAGTACGGGAAGCAGTGGTATCAAC12950.26649819316282936No Hit
CTTCCCGTACTCTGCGTTGATACCA11430.23521809635916138No Hit
GCGTTGATACCACTGCTTCCATGTA11150.22945597326374884No Hit
AAAAAGTACTCTGCGTTGATACCAC11050.22739807215824437No Hit
GTATCAACGCAGAGTACATGGAAGC11020.22678070182659302No Hit
GGTATCAACGCAGAGTACATGGGAA10880.22389964027888673No Hit
CCATGTACTCTGCGTTGATACCACT9850.20270325889219068No Hit
GCAGTGGTATCAACGCAGAGTACGG9630.1981758764600808No Hit
GCAGAGTACGGAAGCAGTGGTATCA9560.1967353456862277No Hit
CACTGCTTCCGTACTCTGCGTTGAT9100.18726900060090712No Hit
GTATCAACGCAGAGTACGGGAAGCA9070.18665163026925577No Hit
GGGAAGCAGTGGTATCAACGCAGAG8670.17842002584723787No Hit
GCCCATGTACTCTGCGTTGATACCA8310.17101158186742177No Hit
ATACCACTGCTTCCCATGTACTCTG8250.1697768412041191No Hit
GCTTCCCGTACTCTGCGTTGATACC8240.16957105109356865No Hit
GCGTTGATACCACTGCTTCCCGTAC8050.16566103899311016No Hit
CTGCGTTGATACCACTGCTTCCGTA8020.1650436686614588No Hit
TGATACCACTGCTTCCGTACTCTGC7960.16380892799815613No Hit
GTACTTTTTTTTTTTTTTTTTTTTT7830.1611336565610003No Hit
CTGCTTCCCATGTACTCTGCGTTGA7430.15290205213898242No Hit
GTATCAACGCAGAGTACTTTTTTTT7190.1479630894857717No Hit
AAAGTACTCTGCGTTGATACCACTG7180.14775729937522122No Hit
TACCACTGCTTCCGTACTCTGCGTT7100.14611097849081764No Hit
TATCAACGCAGAGTACATGGAAGCA7030.14467044771696452No Hit
CCCCATGTACTCTGCGTTGATACCA7010.1442588674958636No Hit
GTGGTATCAACGCAGAGTACATGGG6810.14014306528485468No Hit
ACGCAGAGTACATGGAAGCAGTGGT6780.13952569495320333No Hit
CATGGGAAGCAGTGGTATCAACGCA6750.138908324621552No Hit
TACCACTGCTTCCCATGTACTCTGC6530.13438094218944213No Hit
GCAGTGGTATCAACGCAGAGTACAT6150.12656091798852515No Hit
TCCCATGTACTCTGCGTTGATACCA6020.12388564655136933No Hit
CTTCCCATGTACTCTGCGTTGATAC5790.11915247400870904No Hit
TATCAACGCAGAGTACGGGAAGCAG5640.11606562235045231No Hit
ACGGAAGCAGTGGTATCAACGCAGA5620.11565404212935143No Hit
TCAACGCAGAGTACGGAAGCAGTGG5540.11400772124494786No Hit
GCAGAGTACATGGGAAGCAGTGGTA5510.11339035091329651No Hit
CTCTGCGTTGATACCACTGCTTCCG5380.11071507947614069No Hit
CCACTGCTTCCCATGTACTCTGCGT5370.11050928936559025No Hit
TACGGAAGCAGTGGTATCAACGCAG5310.10927454870228756No Hit
GTTGATACCACTGCTTCCCATGTAC5290.10886296848118668No Hit
ATACCACTGCTTCCATGTACTCTGC5190.10680506737568218No Hit
GATACCACTGCTTCCCATGTACTCT5120.10536453660182905No Hit
ACATGGGAAGCAGTGGTATCAACGC4880.10042557394861834No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATTCTT250.005968462719.0316895
GCAAGAC307.9177634E-418.925853
CAAGACG250.00616387418.9258484
ACTCATT504.53531E-617.10549213
AGAATTT753.254172E-916.47025317
TTGAGCA456.665634E-414.8024269
ACTTTGG400.005214162614.2737678
TATACTA400.005214162614.2737675
ATTGAGC400.005214162614.2737678
CTCATTC602.5533047E-514.25457714
CAATAAC400.00526092614.25457714
GACTCAT602.5533047E-514.25457712
TCGCCGT400.00526453814.25310219
ATCGCCA602.5557969E-514.25310216
CTGCCCG400.005344525414.220751
TTCAAGA400.00541072814.1943872
TTATACT400.00541072814.1943874
CGAGATT400.00541072814.1943874
CGGTCCA759.453688E-713.956573510
CATCGCC551.9504398E-413.8226215
Produced by FastQC (version 0.11.5)