##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4050222_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	485932
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.542063498596512	31.0	31.0	34.0	27.0	34.0
2	30.635428413852143	31.0	31.0	34.0	27.0	34.0
3	30.77846488809134	31.0	31.0	34.0	27.0	34.0
4	34.24189186964431	37.0	35.0	37.0	30.0	37.0
5	34.249300313624126	37.0	35.0	37.0	30.0	37.0
6	34.254990410180845	37.0	35.0	37.0	30.0	37.0
7	34.14194784455438	37.0	35.0	37.0	30.0	37.0
8	34.06986368463077	37.0	35.0	37.0	30.0	37.0
9	35.52177876739955	39.0	37.0	39.0	30.0	39.0
10	35.505807396919735	39.0	37.0	39.0	30.0	39.0
11	35.333021904299365	39.0	37.0	39.0	30.0	39.0
12	35.297220598766906	39.0	37.0	39.0	29.0	39.0
13	35.26643028242635	39.0	37.0	39.0	30.0	39.0
14	36.443426240708575	40.0	37.0	41.0	29.0	41.0
15	36.37366339323197	40.0	37.0	41.0	29.0	41.0
16	36.24049661269478	40.0	37.0	41.0	29.0	41.0
17	36.30991167488455	40.0	37.0	41.0	29.0	41.0
18	36.1973218475013	40.0	37.0	41.0	29.0	41.0
19	36.27828173489295	40.0	37.0	41.0	29.0	41.0
20	36.288916967806195	40.0	37.0	41.0	29.0	41.0
21	36.22869249195361	40.0	37.0	41.0	29.0	41.0
22	36.20196858819752	40.0	37.0	41.0	29.0	41.0
23	36.088129614843226	40.0	37.0	41.0	28.0	41.0
24	36.00892511709457	40.0	37.0	41.0	28.0	41.0
25	35.75798465628936	40.0	36.0	41.0	27.0	41.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.6189579760486481
1101	2	0.6912875775131724
1101	3	0.6372623128231503
1101	4	0.655623004831746
1101	5	0.5459534486458821
1101	6	0.7697310446177568
1101	7	0.7751914555215649
1101	8	0.7723835813056397
1101	9	1.0685270297663934
1101	10	0.7547265904641378
1101	11	0.5023474365451577
1101	12	0.7557912098929336
1101	13	0.9422340293669649
1101	14	1.1316188649790178
1101	15	1.1680329641658602
1101	16	1.2298402660314522
1101	17	1.3560566446944193
1101	18	1.1427903638876131
1101	19	1.2628593185687365
1101	20	1.3086588385102047
1101	21	1.0928738542487082
1101	22	1.1735193430703532
1101	23	1.375324468877615
1101	24	1.319030890288218
1101	25	1.5173622363924864
1102	1	0.5203362682052912
1102	2	0.6070620538137739
1102	3	0.5080600703882716
1102	4	0.44189583440875424
1102	5	0.5389473882901044
1102	6	0.2929844335645768
1102	7	0.2900367460985862
1102	8	0.2000216944391653
1102	9	0.12409440089349744
1102	10	0.2173784865192232
1102	11	0.20351511327112348
1102	12	0.2544087880219692
1102	13	0.197776622208103
1102	14	0.4981268817869591
1102	15	0.39163221668756876
1102	16	0.3176937437671299
1102	17	0.4902930088939286
1102	18	0.4699814354452343
1102	19	0.5397525344805203
1102	20	0.4757388207524329
1102	21	0.4273641958793206
1102	22	0.5642749775493385
1102	23	0.6575904431288464
1102	24	0.7308604569779433
1102	25	0.7361813445806789
1103	1	0.1783080921734026
1103	2	2.1052044615288423E-4
1103	3	0.036308430938611025
1103	4	0.1607884826021504
1103	5	0.16597459993984387
1103	6	0.029794498499640554
1103	7	-0.017621251844062158
1103	8	0.01511748973297955
1103	9	0.18806709280554657
1103	10	0.14682388641635669
1103	11	0.38191777496586354
1103	12	0.48977428885776675
1103	13	0.3092732629436057
1103	14	0.2947969505795527
1103	15	0.4005748520225296
1103	16	0.31875657326436624
1103	17	0.3375388941967401
1103	18	0.4785443453076539
1103	19	0.4083594844612364
1103	20	0.5231723587490862
1103	21	0.7015521057417402
1103	22	0.5662117783722778
1103	23	0.5755878232239979
1103	24	0.6773151470898071
1103	25	0.6261172131675394
1104	1	0.5869539661492098
1104	2	0.4812690943829452
1104	3	0.47710860001131294
1104	4	0.6788073244541408
1104	5	0.7070052118529659
1104	6	0.5470208120644742
1104	7	0.690260950002731
1104	8	0.4549666248610791
1104	9	0.8285742296243797
1104	10	0.7942199229620712
1104	11	0.6530925050998206
1104	12	0.7358482256505852
1104	13	0.6927451871210053
1104	14	0.8824119687873804
1104	15	0.8381581690988753
1104	16	1.0411014002899819
1104	17	0.847321538233679
1104	18	1.0027550684185869
1104	19	0.802506155029576
1104	20	0.9483881702980526
1104	21	0.9944725928011735
1104	22	0.8179268908706518
1104	23	1.0310578941044781
1104	24	1.0329266537876904
1104	25	1.2069103917343185
1105	1	0.5500333203970591
1105	2	0.5058229998426285
1105	3	0.5025424235847389
1105	4	0.4879189663095147
1105	5	0.6099508424908748
1105	6	0.558375945818014
1105	7	0.570795605008982
1105	8	0.4751149539145416
1105	9	0.4702739680432799
1105	10	0.64169479740913
1105	11	0.661927601262299
1105	12	0.7137425277318599
1105	13	0.7068717636409545
1105	14	0.8753666380399281
1105	15	1.0615735682644498
1105	16	0.8981296714005396
1105	17	0.8177634726169316
1105	18	1.0491153799215738
1105	19	0.7484959431907896
1105	20	1.1047801468814598
1105	21	0.9883670385425489
1105	22	0.7959648530521335
1105	23	0.9496864190145402
1105	24	0.9976940142129251
1105	25	1.0262099290290791
1106	1	0.059713087073312465
1106	2	0.17430884060425456
1106	3	0.12268322374748308
1106	4	0.2087950649048409
1106	5	0.09595396051615523
1106	6	0.13810565175732847
1106	7	0.2355941950512559
1106	8	0.27203448578040934
1106	9	0.017399379558064254
1106	10	0.13791267578267252
1106	11	0.3042290716635563
1106	12	0.31276134738129
1106	13	0.23706516336436323
1106	14	0.3835245580001825
1106	15	0.44269640946228606
1106	16	0.5049357298371078
1106	17	0.33051431814165966
1106	18	0.4727071337814479
1106	19	0.4485904228776576
1106	20	0.4746358452976054
1106	21	0.2603844243698106
1106	22	0.38934227690599954
1106	23	0.38614540641920314
1106	24	0.29241977860836244
1106	25	0.2761116349449182
1107	1	-0.02829673623112683
1107	2	0.029614119691618868
1107	3	0.1254300690060326
1107	4	-0.04941026694147865
1107	5	0.029761185676726143
1107	6	0.10843671889638529
1107	7	0.10826486839446403
1107	8	-0.0851624177758481
1107	9	-0.08896718065192033
1107	10	0.0807508822785934
1107	11	0.2995352144206507
1107	12	0.13174368158016847
1107	13	-0.10331458846201969
1107	14	0.08437001556677615
1107	15	0.14851547816346056
1107	16	0.23963516338240964
1107	17	0.2589480155305637
1107	18	0.34140868144005765
1107	19	0.4402560099571602
1107	20	0.537439655207649
1107	21	0.646850344149918
1107	22	0.32797854239807833
1107	23	0.35334848709159417
1107	24	0.43806078114116787
1107	25	0.5498386139220699
1108	1	0.02396688852711648
1108	2	0.18041833701566645
1108	3	0.07465489007292803
1108	4	-0.1149986778840244
1108	5	-0.12549375861156165
1108	6	0.0939978812355946
1108	7	0.08784120009690355
1108	8	-0.017128572526729613
1108	9	-0.036413171984236214
1108	10	-0.08028799811793164
1108	11	-0.09092887020342744
1108	12	0.12519999889124733
1108	13	-0.04498577525557579
1108	14	0.2529022618780772
1108	15	0.17485822979755028
1108	16	-0.10133946729126109
1108	17	-0.05995195908367634
1108	18	0.049001216199378916
1108	19	-0.019129508469433176
1108	20	-0.043689773831836476
1108	21	-0.02226540945111566
1108	22	0.026097986979493726
1108	23	-0.07320721274275854
1108	24	0.1974786253237184
1108	25	0.1220903311137107
1109	1	0.04478917308980357
1109	2	0.13508515485999695
1109	3	0.03550109484307029
1109	4	-0.03438501849461062
1109	5	0.09584679711264243
1109	6	-0.1712055319658674
1109	7	-0.04684529522695158
1109	8	-0.10528221779809854
1109	9	0.03766266311995281
1109	10	-0.1060589127549818
1109	11	0.01887548883376411
1109	12	0.04118484211147688
1109	13	-0.20358699174808237
1109	14	0.11592249895878837
1109	15	-0.17384097176493896
1109	16	-0.014380003630591887
1109	17	0.01979120957573599
1109	18	-0.08815227911350121
1109	19	0.06322330361795281
1109	20	0.11902067095966373
1109	21	-0.046319116534647264
1109	22	-0.11766282557605479
1109	23	-0.2867956461886223
1109	24	-0.2771198699708748
1109	25	-0.10925172419536722
1110	1	-0.014437377226478532
1110	2	0.024038877546569637
1110	3	-0.05683625117665869
1110	4	-0.062167176934394774
1110	5	-0.21258860564542204
1110	6	-0.34903237315811
1110	7	-0.22399751447032656
1110	8	-0.21566088222186863
1110	9	-0.09469991551293333
1110	10	-0.06894594424317546
1110	11	-0.25772126246729243
1110	12	-0.2564601139942866
1110	13	-0.3014089265988815
1110	14	-0.01587409814296592
1110	15	-0.26507614322405004
1110	16	-0.11603063707431716
1110	17	-0.0372642846505542
1110	18	-0.1080755299151619
1110	19	-0.17569800216978138
1110	20	-0.2827824095218574
1110	21	-0.20036597209545448
1110	22	-0.23924722341025273
1110	23	-0.14880128848128749
1110	24	-0.04632381607121516
1110	25	-0.2877233988348422
1111	1	-0.3333800135026479
1111	2	-0.3154836996738304
1111	3	-0.3433927688768037
1111	4	-0.28489535449983805
1111	5	-0.34497804196140436
1111	6	-0.3114390617218632
1111	7	-0.28742109033044727
1111	8	-0.32832967224210563
1111	9	-0.6068610913199706
1111	10	-0.5502587739435825
1111	11	-0.4425226957309505
1111	12	-0.5575549681796161
1111	13	-0.47015249847771656
1111	14	-0.5654577217785501
1111	15	-0.41925815443801184
1111	16	-0.4059131890798895
1111	17	-0.3909884477506509
1111	18	-0.6426629689516901
1111	19	-0.5979135889120926
1111	20	-0.6760590095019481
1111	21	-0.4467015283145628
1111	22	-0.3390431824029889
1111	23	-0.8707016899278202
1111	24	-0.6018943014565679
1111	25	-0.7370432621439562
1112	1	-0.08864366484267094
1112	2	-0.1742055565507279
1112	3	-0.12528607613742793
1112	4	-0.07750682202726011
1112	5	-0.1737988769131178
1112	6	-0.08319347823338319
1112	7	-0.09643961356150044
1112	8	-0.12460426608376451
1112	9	-0.21050345768617973
1112	10	-0.08684650816267947
1112	11	-0.015644590109694434
1112	12	-0.06334096659917776
1112	13	-0.05471915130441829
1112	14	0.05017518876616833
1112	15	-0.064865751239644
1112	16	-0.1727019599570312
1112	17	-0.0015786999505351673
1112	18	0.1500857821030479
1112	19	0.17367340866368153
1112	20	0.3072895031482261
1112	21	-0.004668660629093324
1112	22	-0.1668327167104735
1112	23	-0.20730750539197373
1112	24	0.015596959274986943
1112	25	0.06368467538869282
1113	1	-0.18196403701320207
1113	2	-0.06512856521144883
1113	3	-0.27792747756879876
1113	4	-0.15811723860219473
1113	5	-0.10450802638762013
1113	6	-0.006300363794672137
1113	7	-0.012672453851301668
1113	8	0.10649337956842686
1113	9	-0.15174976155319087
1113	10	-0.14065678519059333
1113	11	0.21597805037723106
1113	12	0.10840685975284714
1113	13	-0.024106871844587374
1113	14	-0.010907113488599407
1113	15	0.06801890908869979
1113	16	0.007093529151866562
1113	17	-0.12339951751990696
1113	18	-0.07774504085175948
1113	19	-0.19064929719356627
1113	20	-0.28477088141895734
1113	21	-0.0921209453727414
1113	22	0.046015431073875845
1113	23	0.0061350720278312565
1113	24	-0.08852137117369807
1113	25	-0.037061833395654276
1114	1	-0.13629361869298862
1114	2	-0.19442335695580226
1114	3	-0.18063307934344408
1114	4	-0.14452496553808913
1114	5	-0.12795414205363898
1114	6	-0.41317837766622034
1114	7	-0.2969819946241543
1114	8	-0.26120569264706717
1114	9	-0.4720123402768195
1114	10	-0.34529777051138666
1114	11	-0.10952086982568687
1114	12	-0.2161828411568436
1114	13	0.023754876046204743
1114	14	-0.4760322858515096
1114	15	-0.2425469172773944
1114	16	-0.4603530879335196
1114	17	-0.26703395106677164
1114	18	-0.27039163064470273
1114	19	-0.07332033911444569
1114	20	-0.2231105803451925
1114	21	-0.04724867243558606
1114	22	-0.07028529995113075
1114	23	-0.0793505827766765
1114	24	-0.03222911350126623
1114	25	0.1434681795682522
1115	1	-0.4469787636107192
1115	2	-0.499709989396802
1115	3	-0.48706858742016124
1115	4	-0.42369572996144456
1115	5	-0.4732679625463305
1115	6	-0.6105609261851583
1115	7	-0.652789079758243
1115	8	-0.6585166620033647
1115	9	-0.7129814860486903
1115	10	-0.9143731662268593
1115	11	-0.7796476972747897
1115	12	-0.6483450431484314
1115	13	-0.8159168222145468
1115	14	-0.9404005997411744
1115	15	-1.0000935565154023
1115	16	-1.0083592473505405
1115	17	-1.051485934381006
1115	18	-1.0576962541172534
1115	19	-1.1522258395821154
1115	20	-1.072606368302452
1115	21	-1.1660069243401026
1115	22	-1.3354830900196717
1115	23	-1.2524127573997532
1115	24	-1.0116260440183495
1115	25	-1.1811779095929182
1116	1	-0.6582049948580497
1116	2	-0.5250008647326503
1116	3	-0.7058016509226555
1116	4	-0.5520844708661841
1116	5	-0.614891675372526
1116	6	-0.6469275501862697
1116	7	-1.0480364823481594
1116	8	-1.0212892315078648
1116	9	-0.9612287387325864
1116	10	-0.9122225913949293
1116	11	-0.9005843792779942
1116	12	-1.0471440652999249
1116	13	-1.001523538004264
1116	14	-1.240512452694901
1116	15	-1.083288515060623
1116	16	-1.1951450316473142
1116	17	-1.2720178701289768
1116	18	-1.342370669772059
1116	19	-1.264909042035967
1116	20	-1.612282455985941
1116	21	-1.4454144496223265
1116	22	-1.4226130816018738
1116	23	-1.8396494128811298
1116	24	-1.7492587973306968
1116	25	-1.7450724680043734
1201	1	0.4354103649006724
1201	2	0.28288097499160614
1201	3	0.20960388548406428
1201	4	0.2105043916930569
1201	5	0.3463723272125705
1201	6	0.32666949849964055
1201	7	0.3587992027013911
1201	8	0.3190947624602529
1201	9	0.44801027462372645
1201	10	0.2618807045981768
1201	11	0.3733950476931369
1201	12	0.30511519794867326
1201	13	0.31353462657997255
1201	14	0.40275149603409943
1201	15	0.38068848838616987
1201	16	0.21790430053709287
1201	17	0.4099820760149271
1201	18	0.5112147998531071
1201	19	0.32171175718850975
1201	20	-0.03222536852364044
1201	21	0.2043930148326467
1201	22	0.25797314200864463
1201	23	0.26592873231491154
1201	24	0.3875424198170805
1201	25	0.1616285768038992
1202	1	0.6086107262547991
1202	2	0.5727686065371884
1202	3	0.5471728280477777
1202	4	0.5506804725856682
1202	5	0.4384027253360614
1202	6	0.4607469360551164
1202	7	0.42993089092251324
1202	8	0.5741386638169104
1202	9	0.6111386821297486
1202	10	0.7323503064746859
1202	11	0.6730852023780614
1202	12	0.7360577299633775
1202	13	0.6761883605726169
1202	14	0.6354782140510835
1202	15	0.6166642237735118
1202	16	0.7614490577324773
1202	17	0.9516546860060515
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2210	18	-0.2386668289347753
2210	19	-0.15792839432664607
2210	20	-0.24647726246303137
2210	21	-0.1588882351673533
2210	22	-0.12720678223378457
2210	23	0.15608024746642712
2210	24	-0.005923489273826021
2210	25	0.051543349531172566
2211	1	-0.16107211254554343
2211	2	-0.027096182075244002
2211	3	-0.00892925122655086
2211	4	-0.0864898831161014
2211	5	-0.18486321523480598
2211	6	-0.20083599537035468
2211	7	-0.11721210310476238
2211	8	-0.12159312789366794
2211	9	-0.02684081258422566
2211	10	0.06045085264351968
2211	11	0.012657423358184872
2211	12	0.08685675473793708
2211	13	0.019236685335464188
2211	14	0.11873648909447354
2211	15	0.2180301781849181
2211	16	0.03520420800875712
2211	17	0.06559955808756257
2211	18	0.03179874040365149
2211	19	-0.03125854699840147
2211	20	0.24543192964892313
2211	21	0.09096267132119351
2211	22	-0.056894282233784566
2211	23	0.031915171130549425
2211	24	0.23763567103151928
2211	25	0.12436062052353947
2212	1	-0.3516350896447804
2212	2	-0.22427811591748537
2212	3	-0.12755520542502552
2212	4	-0.010745608306940824
2212	5	-0.268854945514704
2212	6	-0.11844413786399599
2212	7	-0.2933030700258854
2212	8	-0.3057916011761108
2212	9	-0.21483063446717665
2212	10	-0.29414202267454925
2212	11	-0.0933094977614104
2212	12	-0.31761207477860154
2212	13	-0.33010173705639545
2212	14	-0.33628259487499434
2212	15	-0.321072875250195
2212	16	-0.5000502449174533
2212	17	-0.3550747421668916
2212	18	-0.40236474560143876
2212	19	-0.1083450609933081
2212	20	-0.1057480957963648
2212	21	-0.04998198516735641
2212	22	-0.02856094890044858
2212	23	-0.10537808586691
2212	24	-0.2921734892738286
2212	25	-0.2600191504688283
2213	1	-0.6225243931273674
2213	2	-0.7226736383055439
2213	3	-0.766410926818061
2213	4	-0.7582456083069431
2213	5	-0.7166223584500244
2213	6	-0.7448620483117523
2213	7	-0.7952247118169282
2213	8	-0.42280652654925177
2213	9	-0.8384127240194204
2213	10	-0.8153609281471859
2213	11	-0.7412448211444911
2213	12	-0.7815113285099429
2213	13	-0.9779624335738077
2213	14	-0.9027875699993757
2213	15	-0.8325280991307906
2213	16	-1.1369407921811359
2213	17	-1.082562304355946
2213	18	-1.0829804172432276
2213	19	-0.8294147127346037
2213	20	-0.9177692400749748
2213	21	-0.9738563632768091
2213	22	-0.9250534862138835
2213	23	-0.8764912699465128
2213	24	-0.8083177678807942
2213	25	-0.8123014887772868
2214	1	-0.5155293204140108
2214	2	-0.6205281159174838
2214	3	-0.6048628977327191
2214	4	-0.6272359929223299
2214	5	-0.3302010993608562
2214	6	-0.7075306763255327
2214	7	-0.6422934546412691
2214	8	-0.6803589088684205
2214	9	-0.9983402498517933
2214	10	-0.7622670226745498
2214	11	-0.7826364208383296
2214	12	-0.5673716901632133
2214	13	-0.5464959678256278
2214	14	-0.6641191333365342
2214	15	-0.748572875250197
2214	16	-0.6580790910713006
2214	17	-0.6870458960130463
2214	18	-0.6183743609860528
2214	19	-0.7476719840702302
2214	20	-0.7390654034886737
2214	21	-0.8208473697827401
2214	22	-0.9435128719773687
2214	23	-1.0149934704822954
2214	24	-0.9794811815815194
2214	25	-0.7086729966226741
2215	1	-1.0128697144910284
2215	2	-1.0749023471597958
2215	3	-1.0593879237522081
2215	4	-1.3923169490203477
2215	5	-1.3955662001272486
2215	6	-1.3042743961665764
2215	7	-1.1254325288204683
2215	8	-1.3168740120002198
2215	9	-1.177216858329416
2215	10	-1.010556536819692
2215	11	-1.1979281939483712
2215	12	-0.9595785569434199
2215	13	-1.1721804578436021
2215	14	-1.3972020290693337
2215	15	-1.5556146956684032
2215	16	-1.198629580710815
2215	17	-1.6486479278987503
2215	18	-1.7424001084550653
2215	19	-1.7619797473401704
2215	20	-1.7635725115405236
2215	21	-1.7628771266187684
2215	22	-1.7463161764527229
2215	23	-1.7313544081301302
2215	24	-1.6128192457313872
2215	25	-1.8930003927812535
2216	1	-1.1064544538066308
2216	2	-1.1396393875937854
2216	3	-1.2707921996446778
2216	4	-1.0628698857635897
2216	5	-1.1914922287516987
2216	6	-1.3663054095402956
2216	7	-1.289379659621261
2216	8	-1.2977569190951925
2216	9	-1.3467381489180497
2216	10	-1.434286531345073
2216	11	-1.4531649890908866
2216	12	-1.3564487799809157
2216	13	-1.3105063613338501
2216	14	-1.4248808607709478
2216	15	-1.5169254764062643
2216	16	-1.6615820368249672
2216	17	-1.5173579791611118
2216	18	-1.743686999936699
2216	19	-1.5234028644615165
2216	20	-1.636448962848391
2216	21	-1.7412175343003042
2216	22	-1.6987054575709664
2216	23	-1.7210934037859857
2216	24	-1.6730116395628443
2216	25	-1.914433890353223
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	12180.0
3	0.0
4	83.0
5	620.0
6	1331.0
7	1306.0
8	1206.0
9	1041.0
10	980.0
11	1305.0
12	1390.0
13	1231.0
14	610.0
15	555.0
16	595.0
17	670.0
18	770.0
19	914.0
20	1328.0
21	1693.0
22	1909.0
23	2353.0
24	2590.0
25	2930.0
26	3302.0
27	3546.0
28	4022.0
29	4731.0
30	5999.0
31	7778.0
32	9953.0
33	13689.0
34	18627.0
35	26512.0
36	40783.0
37	77369.0
38	230031.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	51.664082538164	13.132252397878815	12.933082068072544	22.270582995884638
2	9.875757987427598	21.729384594484443	39.540493921692125	28.85436349639583
3	22.303052759422794	30.63443051524321	26.40338417834867	20.659132546985322
4	13.425141490108562	13.604461802302959	32.72935397335519	40.2410427342333
5	17.572201980279544	32.703225673644994	28.739167129829763	20.985405216245702
6	37.776647530248994	28.018927756591356	13.783162697987795	20.421262015171855
7	27.150685439280398	32.47831038239213	21.092213566281785	19.278790612045693
8	26.735766447409066	33.21835008999584	24.488341728826267	15.55754173376883
9	29.722979483927293	21.59499905285092	14.31039310804912	34.37162835517267
10	14.041036663701947	25.33682511447113	28.91034193102085	31.711796290806078
11	31.034489900747296	26.75204221968153	17.134761644380376	25.0787062351908
12	27.52697475043311	23.25261778750127	24.049079246673305	25.171328215392318
13	31.85106400589321	19.474080634347814	27.185743728117735	21.48911163164124
14	33.68872538877256	19.054200564434034	22.089895637636662	25.167178409156744
15	35.76171685045431	17.175540137028538	26.13371353130943	20.929029481207724
16	28.634201744822324	20.420048285692417	32.00013240292694	18.945617566558333
17	25.59296224572554	28.816806527963983	27.690467914659784	17.899763311650695
18	27.494119131803856	17.025449625217494	38.63373518403962	16.846696058939024
19	24.490864320152294	33.85002172698491	19.254454031907628	22.404659920955158
20	19.12919697759681	29.590916238382537	28.958835067159917	22.32105171686074
21	20.041135488463233	33.563910023361146	21.55743759841583	24.837516889759787
22	19.122146801252065	31.64405071985535	17.064678366686596	32.16912411220599
23	23.309393899893028	25.545051448056395	18.084514269516223	33.06104038253435
24	17.63289471234503	29.636601718213768	19.0147473464499	33.7157562229913
25	26.914197290789183	28.83997947818316	18.037063394209206	26.20875983681846
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	271.0
1	271.0
2	299.5
3	328.0
4	328.0
5	328.0
6	774.5
7	1221.0
8	1221.0
9	1221.0
10	1235.5
11	1250.0
12	1250.0
13	1250.0
14	1357.0
15	1464.0
16	1464.0
17	1464.0
18	2166.0
19	2868.0
20	2868.0
21	2868.0
22	3434.5
23	4001.0
24	4001.0
25	4001.0
26	5446.0
27	6891.0
28	6891.0
29	6891.0
30	8157.0
31	9423.0
32	9423.0
33	9423.0
34	11600.5
35	13778.0
36	13778.0
37	13778.0
38	15848.5
39	17919.0
40	17919.0
41	17919.0
42	23194.5
43	28470.0
44	28470.0
45	28470.0
46	63870.5
47	99271.0
48	99271.0
49	99271.0
50	147103.5
51	194936.0
52	194936.0
53	194936.0
54	121071.0
55	47206.0
56	47206.0
57	47206.0
58	33356.0
59	19506.0
60	19506.0
61	19506.0
62	16859.5
63	14213.0
64	14213.0
65	14213.0
66	11895.5
67	9578.0
68	9578.0
69	9578.0
70	7420.5
71	5263.0
72	5263.0
73	5263.0
74	4353.5
75	3444.0
76	3444.0
77	3444.0
78	2987.0
79	2530.0
80	2530.0
81	2530.0
82	1876.5
83	1223.0
84	1223.0
85	1223.0
86	921.0
87	619.0
88	619.0
89	619.0
90	426.0
91	233.0
92	233.0
93	233.0
94	126.5
95	20.0
96	20.0
97	20.0
98	13.0
99	6.0
100	6.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.1893269017064116
2	0.021813751718347426
3	0.029222195698163526
4	0.04301013310504351
5	0.029222195698163526
6	0.06091387272293242
7	0.06811652659219809
8	0.07284969913485838
9	0.054328589185318105
10	0.04527382432109842
11	0.6428883053595976
12	0.5751833589885004
13	0.5482248545063919
14	0.5383469291999704
15	0.5504885457224468
16	0.5266168928985949
17	0.5342311269889614
18	0.5319674357729065
19	0.5478132742852909
20	0.5367006083155668
21	0.545549583069236
22	0.5290863742252003
23	0.5410222006371262
24	0.5192084489187788
25	0.5235300412403382
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	485932.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.996767572343018
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.75733901770367	25.12915358390645
2	5.252940032537759	2.941306823248312
3	1.3285559795993624	1.1158581890306953
4	0.6603297313609942	0.7394839204008584
5	0.4577496424367799	0.6407755172812825
6	0.32439667005430745	0.5449234903651495
7	0.22108982112247696	0.4332860234203817
8	0.19158880133776957	0.42910937124138687
9	0.14958161170347312	0.37690214543627415
>10	1.2504269793417158	7.1950836243650045
>50	0.1982113625111609	3.8422041799114175
>100	0.1466321975454313	7.688740509502198
>500	0.02652642769666094	5.245614383183224
>1k	0.02652642769666094	15.728178850190943
>5k	0.0036842260689806854	7.0968863461644345
>10k+	0.004421071282776823	20.852493042352
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTACGGAAGCAGTGGTATCAACGCA	29698	6.111554703127187	No Hit
CTTCCGTACTCTGCGTTGATACCAC	17077	3.51427771786999	No Hit
GAGTACGGAAGCAGTGGTATCAACG	16160	3.32556818649523	No Hit
TCCGTACTCTGCGTTGATACCACTG	14566	2.9975387502778164	No Hit
GCTTCCGTACTCTGCGTTGATACCA	13397	2.756970111044344	No Hit
GTACATGGGAAGCAGTGGTATCAAC	10184	2.0957664858457563	No Hit
CCCATGTACTCTGCGTTGATACCAC	8686	1.787492900241186	No Hit
GCGTTGATACCACTGCTTCCGTACT	7422	1.5273742005054205	No Hit
GTACATGGAAGCAGTGGTATCAACG	6675	1.3736489879242364	No Hit
GTATCAACGCAGAGTACGGAAGCAG	6550	1.3479252241054305	No Hit
TCCATGTACTCTGCGTTGATACCAC	5069	1.0431500703802177	No Hit
ACTCTGCGTTGATACCACTGCTTCC	4696	0.9663903591449009	No Hit
TATCAACGCAGAGTACGGAAGCAGT	4490	0.9239975963715088	No Hit
GAGTACATGGGAAGCAGTGGTATCA	3751	0.7719187046747281	No Hit
CATGTACTCTGCGTTGATACCACTG	3606	0.7420791386449133	No Hit
CAGTGGTATCAACGCAGAGTACGGA	3419	0.7035963879719797	No Hit
GGTATCAACGCAGAGTACGGAAGCA	3228	0.6642904768568442	No Hit
ACGCAGAGTACGGAAGCAGTGGTAT	2973	0.6118139986664801	No Hit
ATACCACTGCTTCCGTACTCTGCGT	2966	0.610373467892627	No Hit
GAGTACATGGAAGCAGTGGTATCAA	2512	0.5169447577027239	No Hit
GTATCAACGCAGAGTACATGGGAAG	2492	0.5128289554917149	No Hit
GTACGGGAAGCAGTGGTATCAACGC	2445	0.5031568202958439	No Hit
AAGCAGTGGTATCAACGCAGAGTAC	2390	0.49183836421556926	No Hit
GCGTTGATACCACTGCTTCCCATGT	2290	0.47125935316052453	No Hit
GTGGTATCAACGCAGAGTACGGAAG	2208	0.4543845640953878	No Hit
GTACTCTGCGTTGATACCACTGCTT	2132	0.4387445156935539	No Hit
GAAGCAGTGGTATCAACGCAGAGTA	2105	0.43318818270869175	No Hit
GCTTCCCATGTACTCTGCGTTGATA	1947	0.40067334524172105	No Hit
GATACCACTGCTTCCGTACTCTGCG	1689	0.3475794967197056	No Hit
CCCGTACTCTGCGTTGATACCACTG	1685	0.34675633627750385	No Hit
TATCAACGCAGAGTACATGGGAAGC	1662	0.34202316373484354	No Hit
GTTGATACCACTGCTTCCGTACTCT	1658	0.3412000032926418	No Hit
CCACTGCTTCCGTACTCTGCGTTGA	1610	0.3313220779862203	No Hit
TTCCCGTACTCTGCGTTGATACCAC	1587	0.32658890544356	No Hit
CGGAAGCAGTGGTATCAACGCAGAG	1493	0.3072446350518179	No Hit
ACGCAGAGTACATGGGAAGCAGTGG	1475	0.30354041306190993	No Hit
CAGTGGTATCAACGCAGAGTACATG	1454	0.2992188207403505	No Hit
ATCAACGCAGAGTACGGAAGCAGTG	1409	0.28995826576558037	No Hit
GCTTCCATGTACTCTGCGTTGATAC	1379	0.28378456244906697	No Hit
CTGCTTCCGTACTCTGCGTTGATAC	1348	0.2774050690220031	No Hit
CATGGAAGCAGTGGTATCAACGCAG	1295	0.26649819316282936	No Hit
GAGTACGGGAAGCAGTGGTATCAAC	1295	0.26649819316282936	No Hit
CTTCCCGTACTCTGCGTTGATACCA	1143	0.23521809635916138	No Hit
GCGTTGATACCACTGCTTCCATGTA	1115	0.22945597326374884	No Hit
AAAAAGTACTCTGCGTTGATACCAC	1105	0.22739807215824437	No Hit
GTATCAACGCAGAGTACATGGAAGC	1102	0.22678070182659302	No Hit
GGTATCAACGCAGAGTACATGGGAA	1088	0.22389964027888673	No Hit
CCATGTACTCTGCGTTGATACCACT	985	0.20270325889219068	No Hit
GCAGTGGTATCAACGCAGAGTACGG	963	0.1981758764600808	No Hit
GCAGAGTACGGAAGCAGTGGTATCA	956	0.1967353456862277	No Hit
CACTGCTTCCGTACTCTGCGTTGAT	910	0.18726900060090712	No Hit
GTATCAACGCAGAGTACGGGAAGCA	907	0.18665163026925577	No Hit
GGGAAGCAGTGGTATCAACGCAGAG	867	0.17842002584723787	No Hit
GCCCATGTACTCTGCGTTGATACCA	831	0.17101158186742177	No Hit
ATACCACTGCTTCCCATGTACTCTG	825	0.1697768412041191	No Hit
GCTTCCCGTACTCTGCGTTGATACC	824	0.16957105109356865	No Hit
GCGTTGATACCACTGCTTCCCGTAC	805	0.16566103899311016	No Hit
CTGCGTTGATACCACTGCTTCCGTA	802	0.1650436686614588	No Hit
TGATACCACTGCTTCCGTACTCTGC	796	0.16380892799815613	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	783	0.1611336565610003	No Hit
CTGCTTCCCATGTACTCTGCGTTGA	743	0.15290205213898242	No Hit
GTATCAACGCAGAGTACTTTTTTTT	719	0.1479630894857717	No Hit
AAAGTACTCTGCGTTGATACCACTG	718	0.14775729937522122	No Hit
TACCACTGCTTCCGTACTCTGCGTT	710	0.14611097849081764	No Hit
TATCAACGCAGAGTACATGGAAGCA	703	0.14467044771696452	No Hit
CCCCATGTACTCTGCGTTGATACCA	701	0.1442588674958636	No Hit
GTGGTATCAACGCAGAGTACATGGG	681	0.14014306528485468	No Hit
ACGCAGAGTACATGGAAGCAGTGGT	678	0.13952569495320333	No Hit
CATGGGAAGCAGTGGTATCAACGCA	675	0.138908324621552	No Hit
TACCACTGCTTCCCATGTACTCTGC	653	0.13438094218944213	No Hit
GCAGTGGTATCAACGCAGAGTACAT	615	0.12656091798852515	No Hit
TCCCATGTACTCTGCGTTGATACCA	602	0.12388564655136933	No Hit
CTTCCCATGTACTCTGCGTTGATAC	579	0.11915247400870904	No Hit
TATCAACGCAGAGTACGGGAAGCAG	564	0.11606562235045231	No Hit
ACGGAAGCAGTGGTATCAACGCAGA	562	0.11565404212935143	No Hit
TCAACGCAGAGTACGGAAGCAGTGG	554	0.11400772124494786	No Hit
GCAGAGTACATGGGAAGCAGTGGTA	551	0.11339035091329651	No Hit
CTCTGCGTTGATACCACTGCTTCCG	538	0.11071507947614069	No Hit
CCACTGCTTCCCATGTACTCTGCGT	537	0.11050928936559025	No Hit
TACGGAAGCAGTGGTATCAACGCAG	531	0.10927454870228756	No Hit
GTTGATACCACTGCTTCCCATGTAC	529	0.10886296848118668	No Hit
ATACCACTGCTTCCATGTACTCTGC	519	0.10680506737568218	No Hit
GATACCACTGCTTCCCATGTACTCT	512	0.10536453660182905	No Hit
ACATGGGAAGCAGTGGTATCAACGC	488	0.10042557394861834	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	2.057901105504474E-4	0.0
9	0.0	0.0	0.0	2.057901105504474E-4	0.0
10	0.0	0.0	0.0	0.001028950552752237	0.0
11	0.0	0.0	0.0	0.0014405307738531317	0.0
12	0.0	0.0	0.0	0.0018521109949540264	0.0
13	0.0	0.0	0.0	0.0028810615477062635	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATTCTT	25	0.0059684627	19.031689	5
GCAAGAC	30	7.9177634E-4	18.92585	3
CAAGACG	25	0.006163874	18.925848	4
ACTCATT	50	4.53531E-6	17.105492	13
AGAATTT	75	3.254172E-9	16.470253	17
TTGAGCA	45	6.665634E-4	14.802426	9
ACTTTGG	40	0.0052141626	14.273767	8
TATACTA	40	0.0052141626	14.273767	5
ATTGAGC	40	0.0052141626	14.273767	8
CTCATTC	60	2.5533047E-5	14.254577	14
CAATAAC	40	0.005260926	14.254577	14
GACTCAT	60	2.5533047E-5	14.254577	12
TCGCCGT	40	0.005264538	14.253102	19
ATCGCCA	60	2.5557969E-5	14.253102	16
CTGCCCG	40	0.0053445254	14.22075	1
TTCAAGA	40	0.005410728	14.194387	2
TTATACT	40	0.005410728	14.194387	4
CGAGATT	40	0.005410728	14.194387	4
CGGTCCA	75	9.453688E-7	13.9565735	10
CATCGCC	55	1.9504398E-4	13.82262	15
>>END_MODULE