FastQCFastQC Report
Thu 2 Feb 2017
SRR4050222_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4050222_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences485932
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACGGAAGCAGTGGTATCAACGCA266985.4941843714758445No Hit
CTTCCGTACTCTGCGTTGATACCAC165243.400475786735593No Hit
GAGTACGGAAGCAGTGGTATCAACG161203.317336582073212No Hit
TCCGTACTCTGCGTTGATACCACTG149243.0712116098548767No Hit
GCTTCCGTACTCTGCGTTGATACCA128662.647695562342056No Hit
CCCATGTACTCTGCGTTGATACCAC95831.972086629404937No Hit
GTACATGGGAAGCAGTGGTATCAAC87811.8070429607434786No Hit
GCGTTGATACCACTGCTTCCGTACT70081.4421770947375352No Hit
GTATCAACGCAGAGTACGGAAGCAG65831.3547162977535951No Hit
TCCATGTACTCTGCGTTGATACCAC56781.1684762477054402No Hit
GTACATGGAAGCAGTGGTATCAACG55941.1511898784192027No Hit
TATCAACGCAGAGTACGGAAGCAGT51071.0509700945811349No Hit
ACTCTGCGTTGATACCACTGCTTCC49221.012898924129302No Hit
CAGTGGTATCAACGCAGAGTACGGA38820.7988772091568367No Hit
CATGTACTCTGCGTTGATACCACTG38610.7945556168352774No Hit
ACGCAGAGTACGGAAGCAGTGGTAT35210.7245869792481253No Hit
GAGTACATGGGAAGCAGTGGTATCA34880.7177959055999604No Hit
GGTATCAACGCAGAGTACGGAAGCA33040.6799305252586781No Hit
ATACCACTGCTTCCGTACTCTGCGT29820.6136661096614341No Hit
AAGCAGTGGTATCAACGCAGAGTAC27960.5753891490990509No Hit
GCGTTGATACCACTGCTTCCCATGT24060.4951310059843764No Hit
GAAGCAGTGGTATCAACGCAGAGTA23960.49307310487887196No Hit
GTGGTATCAACGCAGAGTACGGAAG23150.4764041059242857No Hit
GTATCAACGCAGAGTACATGGGAAG22290.45870615641694723No Hit
GAGTACATGGAAGCAGTGGTATCAA21720.4469761201155717No Hit
GTACGGGAAGCAGTGGTATCAACGC20680.4255739486183252No Hit
GCTTCCCATGTACTCTGCGTTGATA20430.420429195854564No Hit
ATCAACGCAGAGTACGGAAGCAGTG18020.3708337792119062No Hit
GATACCACTGCTTCCGTACTCTGCG17160.3531358297045677No Hit
TATCAACGCAGAGTACATGGGAAGC17010.350048978046311No Hit
GTACTCTGCGTTGATACCACTGCTT16950.3488142373830083No Hit
CCCGTACTCTGCGTTGATACCACTG16710.34387527472979756No Hit
CGGAAGCAGTGGTATCAACGCAGAG16350.33646683074998146No Hit
CCACTGCTTCCGTACTCTGCGTTGA16280.3350262999761283No Hit
ACGCAGAGTACATGGGAAGCAGTGG16050.33029312743346806No Hit
GTTGATACCACTGCTTCCGTACTCT16030.3298815472123672No Hit
TTCCCGTACTCTGCGTTGATACCAC15450.3179457208004412No Hit
CAGTGGTATCAACGCAGAGTACATG15000.30868516582567107No Hit
CTGCTTCCGTACTCTGCGTTGATAC14340.29510301852934157No Hit
GCTTCCATGTACTCTGCGTTGATAC14220.2926335372027362No Hit
CATGGAAGCAGTGGTATCAACGCAG13950.28707720421787414No Hit
GAGTACGGGAAGCAGTGGTATCAAC13140.27040820526328785No Hit
GTACTTTTTTTTTTTTTTTTTTTTT12340.25394499641925206No Hit
GCGTTGATACCACTGCTTCCATGTA11660.23995126890182164No Hit
AAAAAGTACTCTGCGTTGATACCAC11500.2366586271330145No Hit
GCAGTGGTATCAACGCAGAGTACGG11400.23460072602751003No Hit
CCATGTACTCTGCGTTGATACCACT11180.2300733435954002No Hit
GGTATCAACGCAGAGTACATGGGAA11110.22863281282154707No Hit
GTATCAACGCAGAGTACTTTTTTTT10680.21978383806787782No Hit
GCAGAGTACGGAAGCAGTGGTATCA10570.2175201468518229No Hit
CTTCCCGTACTCTGCGTTGATACCA10240.2107290732036581No Hit
GTATCAACGCAGAGTACATGGAAGC9490.19529481491237455No Hit
CACTGCTTCCGTACTCTGCGTTGAT9060.18644584015870533No Hit
GTATCAACGCAGAGTACGGGAAGCA8990.1850053093848522No Hit
ATACCACTGCTTCCCATGTACTCTG8970.1845937291637513No Hit
GGGAAGCAGTGGTATCAACGCAGAG8950.18418214894265042No Hit
CTGCGTTGATACCACTGCTTCCGTA8590.1767737049628343No Hit
GCTTCCCGTACTCTGCGTTGATACC8430.17348106319402715No Hit
CTGCTTCCCATGTACTCTGCGTTGA8380.1724521126412749No Hit
GCGTTGATACCACTGCTTCCCGTAC8320.17121737197797224No Hit
TGATACCACTGCTTCCGTACTCTGC8220.16915947087246774No Hit
GCCCATGTACTCTGCGTTGATACCA8130.16730735987751372No Hit
CATGGGAAGCAGTGGTATCAACGCA8000.16463208844035793No Hit
CCCCATGTACTCTGCGTTGATACCA7880.16216260711375255No Hit
TATCAACGCAGAGTACATGGAAGCA7870.16195681700320208No Hit
AAAGTACTCTGCGTTGATACCACTG7830.1611336565610003No Hit
TATCAACGCAGAGTACTTTTTTTTT7540.15516574335503733No Hit
GCAGTGGTATCAACGCAGAGTACAT7250.14919783014907434No Hit
ACGGAAGCAGTGGTATCAACGCAGA7160.14734571915412034No Hit
ACGCAGAGTACATGGAAGCAGTGGT7150.1471399290435699No Hit
TACCACTGCTTCCGTACTCTGCGTT7140.14693413893301943No Hit
TACCACTGCTTCCCATGTACTCTGC7130.146728348822469No Hit
TCAACGCAGAGTACGGAAGCAGTGG7100.14611097849081764No Hit
GTGGTATCAACGCAGAGTACATGGG7050.1450820279380654No Hit
TATCAACGCAGAGTACGGGAAGCAG6720.13829095428990065No Hit
TACGGAAGCAGTGGTATCAACGCAG6700.13787937406879977No Hit
TCCCATGTACTCTGCGTTGATACCA6620.13623305318439619No Hit
CTTCCCATGTACTCTGCGTTGATAC5910.1216219553353144No Hit
GAGTACTTTTTTTTTTTTTTTTTTT5850.12038721467201173No Hit
GCAGAGTACATGGGAAGCAGTGGTA5840.12018142456146128No Hit
ACATGGGAAGCAGTGGTATCAACGC5480.11277298058164516No Hit
GATACCACTGCTTCCCATGTACTCT5400.11112665969724159No Hit
CCACTGCTTCCCATGTACTCTGCGT5400.11112665969724159No Hit
CTCTGCGTTGATACCACTGCTTCCG5380.11071507947614069No Hit
GTTGATACCACTGCTTCCCATGTAC5290.10886296848118668No Hit
GGTATCAACGCAGAGTACTTTTTTT5030.10351242560687503No Hit
ATACCACTGCTTCCATGTACTCTGC5000.1028950552752237No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGCAC250.00597771619.0266063
AAACGCT659.969881E-917.53950719
CGTCTTG453.5196583E-516.8951155
TAAACGC753.2923708E-916.45578618
TACCCTA350.0021619816.291725
AGAACCG350.0021619816.291725
GAACCGC350.002169431416.283336
AACCGCG350.002172418216.2799787
GTAAACG702.5140253E-816.27327717
AGTAAAC702.5140253E-816.27327716
GTTATCA601.4159668E-615.8817111
GGACGGA551.09304565E-515.5929521
TCAAAGT807.727067E-915.42888512
CCGTCTT508.591097E-515.2212854
TCCGTCT508.591097E-515.2212853
TTTCAAA755.856964E-815.19151910
GCGCCGA456.747439E-414.77865919
GCACTTA456.779124E-414.76953211
TGCACTG400.00525930814.2552545
CGCGGTC400.00529175114.24204810
Produced by FastQC (version 0.11.5)