##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4050222_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	485932
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.90373550208671	31.0	31.0	34.0	28.0	34.0
2	30.722335635438704	31.0	31.0	34.0	27.0	34.0
3	31.14757003037462	31.0	31.0	34.0	27.0	34.0
4	34.686579603730564	37.0	35.0	37.0	32.0	37.0
5	34.5972790431583	37.0	35.0	37.0	32.0	37.0
6	34.626268696031545	37.0	35.0	37.0	32.0	37.0
7	34.509886156910845	37.0	35.0	37.0	32.0	37.0
8	34.526841204119094	37.0	35.0	37.0	32.0	37.0
9	36.14382053456039	39.0	37.0	39.0	32.0	39.0
10	36.09293892972679	39.0	37.0	39.0	32.0	39.0
11	36.082235786077064	39.0	37.0	39.0	32.0	39.0
12	36.04873315607945	39.0	37.0	39.0	32.0	39.0
13	35.93278483409201	39.0	37.0	39.0	31.0	39.0
14	37.159979174040814	40.0	38.0	41.0	31.0	41.0
15	37.11660067663788	40.0	37.0	41.0	32.0	41.0
16	36.95186980894446	40.0	37.0	41.0	31.0	41.0
17	36.99481614711524	40.0	37.0	41.0	31.0	41.0
18	36.883384918054375	40.0	37.0	41.0	31.0	41.0
19	36.983610875595765	40.0	37.0	41.0	31.0	41.0
20	36.98690145946346	40.0	37.0	41.0	31.0	41.0
21	36.9509128849304	40.0	37.0	41.0	31.0	41.0
22	36.92562539614596	40.0	37.0	41.0	31.0	41.0
23	36.84242239654931	40.0	37.0	41.0	31.0	41.0
24	36.76319320398739	40.0	37.0	41.0	31.0	41.0
25	36.50952602421738	40.0	37.0	41.0	30.0	41.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.24774963519637794
1101	2	0.3328042632708197
1101	3	0.26939756358228095
1101	4	0.22742672759225258
1101	5	0.2566583436557366
1101	6	0.25068533355651823
1101	7	0.49375815489050723
1101	8	0.5331731330989413
1101	9	0.5340501763705134
1101	10	0.2557345691228292
1101	11	0.28800295040510804
1101	12	0.21138769178681827
1101	13	0.32717955945424393
1101	14	0.3782517423192999
1101	15	0.6068217330042032
1101	16	0.6399777992959201
1101	17	0.6162184746983925
1101	18	0.32002168756920213
1101	19	0.4198326161183914
1101	20	0.5045480020922142
1101	21	0.20261133233979933
1101	22	0.7169678217006563
1101	23	0.767365476236769
1101	24	0.9207739378868709
1101	25	0.8561374264334631
1102	1	0.3236992113844437
1102	2	0.6288923913745563
1102	3	0.24765255279340437
1102	4	0.38118425141554013
1102	5	0.25700936937353447
1102	6	0.2962133691546569
1102	7	0.28470169137137447
1102	8	0.535190498547685
1102	9	0.6691104185782564
1102	10	0.44878838962479506
1102	11	0.5503698961716381
1102	12	0.542679569671165
1102	13	0.7204336711010697
1102	14	0.7798933037642826
1102	15	0.5436866603728561
1102	16	0.7251118601472584
1102	17	0.5790758240412544
1102	18	0.5731442678147189
1102	19	0.6069169345606653
1102	20	0.5657606927043375
1102	21	0.7030707630586832
1102	22	0.6875849042639715
1102	23	0.35804677585787203
1102	24	0.47591904572403365
1102	25	0.5449221547498837
1103	1	0.13735646996744677
1103	2	0.4303863800591401
1103	3	0.3830919895942273
1103	4	0.2936344250043277
1103	5	0.2366890337685419
1103	6	0.3657794775512144
1103	7	0.26123907725281725
1103	8	0.44743574092906613
1103	9	0.40564523277396347
1103	10	0.33194143707239476
1103	11	0.19395434390212074
1103	12	0.37779031288834375
1103	13	0.45925487464999293
1103	14	0.35089192148319626
1103	15	0.4230605352603405
1103	16	0.49093375483672475
1103	17	0.3984410194893684
1103	18	0.4757677890954142
1103	19	0.49738321333140334
1103	20	0.5881785594910198
1103	21	0.5710689468056245
1103	22	0.5695827022580531
1103	23	0.38749860496935185
1103	24	0.6162326306539541
1103	25	0.3079352864201965
1104	1	0.4169360641929636
1104	2	0.6637107720476969
1104	3	0.5203253749499304
1104	4	0.4583990237818014
1104	5	0.5305219444201228
1104	6	0.6280189592783572
1104	7	0.4286495012351281
1104	8	0.46020967121908996
1104	9	0.7559793139288615
1104	10	0.6671565170567888
1104	11	0.7285614124415929
1104	12	0.7116560626607438
1104	13	0.6799191352820699
1104	14	0.7397713271276274
1104	15	0.7705029042289979
1104	16	0.5959315601430077
1104	17	0.774910245006339
1104	18	0.6315971394660735
1104	19	0.868770097191458
1104	20	0.8043193125148207
1104	21	0.878352484977043
1104	22	0.8279814699781767
1104	23	0.9073422940056375
1104	24	0.9939190983350557
1104	25	0.791603107333188
1105	1	0.48030214005746785
1105	2	0.7095443298499333
1105	3	0.6737364044541216
1105	4	0.644480754407148
1105	5	0.53179094197192
1105	6	0.5682982584565011
1105	7	0.6511251239118749
1105	8	0.6875817089777527
1105	9	0.545816559007001
1105	10	0.651300033496895
1105	11	0.7080479473001162
1105	12	0.922884296659106
1105	13	0.7718975144369864
1105	14	0.7478941086265962
1105	15	0.5967819280586184
1105	16	0.7960620514006962
1105	17	0.743496569761966
1105	18	1.0015637983511567
1105	19	0.9774968496950365
1105	20	0.8824404778376689
1105	21	0.6251036716693292
1105	22	0.7692843473702666
1105	23	0.7925698456185231
1105	24	0.6312850903978386
1105	25	0.5664877303152096
1106	1	0.2568977806903838
1106	2	0.6057457769735848
1106	3	0.3911325990548917
1106	4	0.18941728870444763
1106	5	0.07834321584173409
1106	6	0.07608843139540511
1106	7	-0.14582374947165277
1106	8	-0.023633205902498844
1106	9	-0.015379088276652908
1106	10	0.22230944038745548
1106	11	0.24298293627749246
1106	12	0.0716614400080644
1106	13	0.18009670813208345
1106	14	0.19789322838106926
1106	15	0.22789645582645335
1106	16	0.4414130835374763
1106	17	0.4322789163400671
1106	18	0.4582860378522682
1106	19	0.28464972487034856
1106	20	0.3475778646038563
1106	21	0.35224125013343865
1106	22	0.2025177239334326
1106	23	0.3812330656349161
1106	24	0.4274456550068848
1106	25	0.2737826184360017
1107	1	0.10701540875288984
1107	2	0.451261547395152
1107	3	0.16332656425651138
1107	4	0.26891433251315533
1107	5	0.23167275653840846
1107	6	0.36360220786641406
1107	7	0.18149205887628028
1107	8	0.16712890753488807
1107	9	0.027066523999472736
1107	10	0.23567379072945016
1107	11	0.15173622901834705
1107	12	0.26212690166769903
1107	13	0.002359109772307022
1107	14	-0.07254805052605207
1107	15	0.23214345785983426
1107	16	0.09523923433045667
1107	17	0.1393953671831767
1107	18	0.2581606179867393
1107	19	-0.0693572333036272
1107	20	0.2568418490127442
1107	21	0.32608332249018446
1107	22	0.0802408652129003
1107	23	-0.07540414238369664
1107	24	0.010845171727339675
1107	25	0.20335598436348334
1108	1	-0.013355113810305852
1108	2	0.2266432013415418
1108	3	0.1184649897182517
1108	4	0.15722427989705068
1108	5	0.326105969152394
1108	6	0.3456276265032159
1108	7	0.08072701598529619
1108	8	0.07828421544227382
1108	9	-0.022355356336788645
1108	10	-0.15568336138971262
1108	11	-0.2655326646554883
1108	12	-0.14166196914067797
1108	13	-0.14240548749808823
1108	14	0.00928907514808941
1108	15	0.09754777636167233
1108	16	-0.03823691674953267
1108	17	0.10037617079781569
1108	18	0.017154757345444693
1108	19	0.023881570022460608
1108	20	0.19134658007889271
1108	21	0.09195718629464977
1108	22	0.029063897222705748
1108	23	0.005574615759378787
1108	24	-0.039451512965044344
1108	25	-0.21278242100880362
1109	1	0.14462249482730627
1109	2	0.5467570234907768
1109	3	0.3876770493066317
1109	4	0.36544163923781525
1109	5	0.36286957239881446
1109	6	0.19075891332990835
1109	7	0.13688646940489946
1109	8	0.19756521935948257
1109	9	0.21231870340520942
1109	10	0.30050864399416355
1109	11	0.07304433902763918
1109	12	0.1580501837145647
1109	13	-0.03920661694152017
1109	14	-0.11525327715194322
1109	15	-0.2767284910618599
1109	16	-0.1378934752388261
1109	17	-0.3469701126090925
1109	18	-0.22284831497477597
1109	19	-0.1149964478920964
1109	20	-0.07387512074295444
1109	21	-0.13730373781537963
1109	22	-0.1690422368109168
1109	23	0.07581736563228247
1109	24	0.0038682021869789196
1109	25	-0.15091723003118318
1110	1	0.12663900116174176
1110	2	0.03385860228136295
1110	3	0.10243988026564566
1110	4	0.1732690061094999
1110	5	0.0542729612789401
1110	6	0.06679143536464238
1110	7	0.14876507249940119
1110	8	0.16752263932478684
1110	9	0.38318905333248665
1110	10	0.36429809185552386
1110	11	0.21797766535191698
1110	12	0.09420816790913733
1110	13	0.24045659776348316
1110	14	0.44829611043150663
1110	15	0.274146765206865
1110	16	0.31681573641724015
1110	17	0.2360171484257947
1110	18	0.24899454191776016
1110	19	0.22586286276693102
1110	20	0.102420241012112
1110	21	0.3422420751478725
1110	22	0.28072909263238444
1110	23	0.26498486462472925
1110	24	0.23135072334557805
1110	25	0.1199320927184715
1111	1	-0.46309922915576607
1111	2	-0.06247529506776317
1111	3	-0.172947465874163
1111	4	-0.023451407944449443
1111	5	-0.17033969258125126
1111	6	-0.26002088008607416
1111	7	-0.3295402259313107
1111	8	-0.4368368709767907
1111	9	-0.2751202263861643
1111	10	-0.2935632929691323
1111	11	-0.2570580003526075
1111	12	-0.27811358651175055
1111	13	-0.30052246729739807
1111	14	-0.4238398505518717
1111	15	-0.5643572658471783
1111	16	-0.6108558736819631
1111	17	-0.6055607686970603
1111	18	-0.5944545805400239
1111	19	-0.4565133025892294
1111	20	-0.27449160663726957
1111	21	-0.25620522255293565
1111	22	-0.544666547857311
1111	23	-0.30416181265870534
1111	24	-0.26276194710331424
1111	25	-0.4060341990435816
1112	1	-0.023068691941883657
1112	2	0.26968387346283507
1112	3	0.05116286990125474
1112	4	0.15796040245695764
1112	5	0.22428348087816374
1112	6	0.25852578961645634
1112	7	0.2171562636284463
1112	8	0.17667986422002713
1112	9	0.034745436659783024
1112	10	0.09842470644154844
1112	11	0.08279909084383519
1112	12	0.1434640356036212
1112	13	0.3454835865713193
1112	14	0.3356829385136635
1112	15	0.49309756452395703
1112	16	-0.044487659171430494
1112	17	0.3677794399738943
1112	18	0.3230145808920071
1112	19	0.40097969929925625
1112	20	0.3371366429642748
1112	21	0.5642814977770811
1112	22	0.5216882731383592
1112	23	0.6331397959024301
1112	24	0.6800443960653269
1112	25	0.5861607301716916
1113	1	-0.015906746277156714
1113	2	0.10380582806940808
1113	3	-0.058361213813405755
1113	4	0.18894017830109533
1113	5	0.12393156498572466
1113	6	0.0492442196448053
1113	7	0.18126693931373694
1113	8	0.050857231184636476
1113	9	-0.078633125260815
1113	10	-0.04768643665849481
1113	11	-0.09790074420617145
1113	12	0.03429559355119949
1113	13	0.012958632381355528
1113	14	-0.10790213266547966
1113	15	-0.2097164077901894
1113	16	-0.10971064649365303
1113	17	0.2825490688533563
1113	18	-0.10558104098977594
1113	19	-0.10979454953474743
1113	20	0.07182965646849482
1113	21	-0.006425145270462451
1113	22	0.0813784923152383
1113	23	-0.0265721004805215
1113	24	0.1771535374108737
1113	25	0.2344345132844694
1114	1	-0.2000339671603193
1114	2	-0.49664544935989596
1114	3	-0.13044183443094326
1114	4	-0.17803373995802474
1114	5	-0.11581194948799833
1114	6	-0.03275371438046193
1114	7	-0.03964796061660536
1114	8	-0.2639468582393789
1114	9	-0.17476759451000135
1114	10	-0.16573737515369658
1114	11	-0.07489257710023622
1114	12	0.1802435379698082
1114	13	-0.02545548606469339
1114	14	0.008047922157430776
1114	15	-0.22478437708935672
1114	16	-0.16699304873670684
1114	17	-0.21613470531116974
1114	18	-0.3393360849353755
1114	19	-0.08554394140508492
1114	20	-0.14943591966394365
1114	21	-0.19854175383489547
1114	22	0.05779591441672238
1114	23	-0.025091643935020613
1114	24	0.04137520285308227
1114	25	-0.1849694641135713
1115	1	-0.5610094888912123
1115	2	-1.1131811381890344
1115	3	-0.7799941639116348
1115	4	-0.6066365327483823
1115	5	-0.5851908047627532
1115	6	-0.6381224523315581
1115	7	-0.7057552271046248
1115	8	-0.7549047257818344
1115	9	-0.9569473905394617
1115	10	-0.8403724868382412
1115	11	-1.1185663926605827
1115	12	-0.9287582936146421
1115	13	-0.992041851737973
1115	14	-0.8847652893108702
1115	15	-0.9912317949763292
1115	16	-1.0826499379303627
1115	17	-1.2399859048636515
1115	18	-1.4225573878565854
1115	19	-1.5517002305969356
1115	20	-1.243627180389538
1115	21	-1.204931246735626
1115	22	-1.1139324216083466
1115	23	-1.2735104361719891
1115	24	-1.172413410045003
1115	25	-1.0745195078731058
1116	1	-0.46327364636172064
1116	2	-0.9056621981399111
1116	3	-0.43910210777759673
1116	4	-0.037849849015486825
1116	5	-0.3461281786399013
1116	6	-0.29838063662371184
1116	7	-0.21453914334175295
1116	8	-0.10115592214719271
1116	9	-0.18527732215925852
1116	10	-0.2756765077789609
1116	11	-0.5374787668260126
1116	12	-0.3217599887058
1116	13	-0.38297369062359365
1116	14	-0.49880917718117246
1116	15	-0.48824520579954367
1116	16	-0.5529253037587054
1116	17	-0.5746107362193769
1116	18	-0.3795545396422
1116	19	-0.8649202119163348
1116	20	-0.8387252535206144
1116	21	-0.5475962298423696
1116	22	-0.7507735558203592
1116	23	-0.9221941460000593
1116	24	-0.8992644611168359
1116	25	-0.8547976411972158
1201	1	0.28934510633108346
1201	2	0.7173181982409567
1201	3	0.5578078986851374
1201	4	0.2737480613679679
1201	5	0.26651857922308864
1201	6	0.39276811391484756
1201	7	0.265500440889177
1201	8	0.160503922747246
1201	9	0.21956568731941672
1201	10	0.22824825525421488
1201	11	0.283442980265761
1201	12	0.3351766765247035
1201	13	0.29874351101363317
1201	14	0.27276692148319626
1201	15	0.467094626169434
1201	16	0.40570648210945137
1201	17	0.47088420130754827
1201	18	0.5212223345499609
1201	19	0.46897412242230985
1201	20	0.5895990140364731
1201	21	0.5071484922601712
1201	22	0.3977077022580531
1201	23	0.45852133224207847
1201	24	0.3989030851994073
1201	25	0.39174210460200953
1202	1	0.5709576491230202
1202	2	0.8378567426811756
1202	3	0.6277112998516117
1202	4	0.42830223278929225
1202	5	0.4690294356957665
1202	6	0.6171127633379498
1202	7	0.4419201029913751
1202	8	0.4128257815021641
1202	9	0.5583688602622345
1202	10	0.47556781592366804
1202	11	0.470948923590214
1202	12	0.5658410609547815
1202	13	0.5347984907270842
1202	14	0.3587727697144629
1202	15	0.6469911330795384
1202	16	0.6231192341064116
1202	17	0.718391967251506
1202	18	0.7477283729676074
1202	19	0.7780547297508278
1202	20	0.5532912449227396
1202	21	0.6669446758534292
1202	22	0.49871252031308444
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2210	19	0.18973643302837218
2210	20	0.36623253676374645
2210	21	0.5362678104419913
2210	22	0.43996622498532645
2210	23	0.6198612943632895
2210	24	0.5281644488357742
2210	25	0.5549760061171654
2211	1	-0.0941812353298026
2211	2	-0.5300938998390805
2211	3	-0.2632039854231216
2211	4	-0.24681780724179703
2211	5	-0.05972693893559722
2211	6	-0.2983535030178359
2211	7	-0.24248374254824512
2211	8	-0.22919940827287633
2211	9	-0.19650460530144898
2211	10	-0.05351860225214722
2211	11	-0.16319780738170664
2211	12	-0.06101809562426297
2211	13	-0.1613644869044819
2211	14	-0.15592481457971985
2211	15	-0.1277541478301032
2211	16	-0.19513856184150313
2211	17	-0.25969207674010164
2211	18	-0.1725341713487225
2211	19	-0.15863546582659183
2211	20	-0.058833064381289546
2211	21	0.030642015276598045
2211	22	-0.07288563570169515
2211	23	-0.08639466937716378
2211	24	-0.10240170892504352
2211	25	-0.12078536474797374
2212	1	-0.4191208027598279
2212	2	-1.0396136199408588
2212	3	-0.4433852831330469
2212	4	-0.23045648408658082
2212	5	-0.25115187532236405
2212	6	-0.31655006790333573
2212	7	-0.4702381954744581
2212	8	-0.404609713616388
2212	9	-0.413559312680583
2212	10	-0.4623767447457823
2212	11	-0.20911383791605687
2212	12	-0.24414150529347722
2212	13	-0.28960876171363736
2212	14	-0.37694898760771167
2212	15	-0.2631326465578425
2212	16	-0.22400942698145343
2212	17	-1.4944567077833568
2212	18	-0.916675392722766
2212	19	-0.5275031503049661
2212	20	-0.5907987132362535
2212	21	-0.36216968955800866
2212	22	-0.3208150250146744
2212	23	-0.24914912230337194
2212	24	-0.4418355511642247
2212	25	-0.2600760772161692
2213	1	-0.8558310017648019
2213	2	-1.622940734368722
2213	3	-1.0715382682076715
2213	4	-1.1882176781164304
2213	5	-1.075231477312414
2213	6	-1.0846035504903995
2213	7	-1.1310466531858978
2213	8	-1.1279119524223589
2213	9	-1.1772782181532193
2213	10	-1.1348580880293682
2213	11	-1.257297917518052
2213	12	-1.2558454853929817
2213	13	-1.339111249275831
2213	14	-1.4756430174584594
2213	15	-1.3284871241697829
2213	16	-1.1631325613098156
2213	17	-1.880215414251019
2213	18	-1.4663893230710272
2213	19	-1.5461349911009847
2213	20	-1.6414827928382465
2213	21	-1.5792094905530334
2213	22	-1.3438685076017407
2213	23	-1.2647585750396928
2213	24	-1.2557285859900986
2213	25	-1.2426569229873152
2214	1	-0.5800823412213667
2214	2	-1.8280270814793198
2214	3	-0.757423744671506
2214	4	-0.4724276379327321
2214	5	-0.5762480291685179
2214	6	-0.5126077602110257
2214	7	-0.3924978108590693
2214	8	-0.4878789443856206
2214	9	-0.5448573896036635
2214	10	-0.5854536678227049
2214	11	-0.7282484533006723
2214	12	-0.5738049668319363
2214	13	-0.6098010694059468
2214	14	-0.5317566799154037
2214	15	-0.5027480311732262
2214	16	-0.5935286577506886
2214	17	-0.48387978470643844
2214	18	-0.7120600081073789
2214	19	-0.643897381074197
2214	20	-0.626856405543947
2214	21	-0.6121696895580087
2214	22	-0.7286034865531335
2214	23	-0.5945818146110682
2214	24	-0.5451528588565324
2214	25	-0.729979923370017
2215	1	-0.8605399294510931
2215	2	-2.0946366826714886
2215	3	-0.9979532413126293
2215	4	-0.7613666931886698
2215	5	-0.8330337941415493
2215	6	-0.9093222081247347
2215	7	-0.8207209753022511
2215	8	-0.7400817308365575
2215	9	-1.0800384024714802
2215	10	-0.9626150596289307
2215	11	-1.0506329031067096
2215	12	-0.8924594634730596
2215	13	-1.0265091307173293
2215	14	-1.0766876099939324
2215	15	-1.0755819085504612
2215	16	-1.241141960806793
2215	17	-1.2607389956062391
2215	18	-1.322459525564092
2215	19	-1.1233641589150523
2215	20	-1.1783602138512563
2215	21	-1.167981497676088
2215	22	-1.3161886412508395
2215	23	-1.1338815946281002
2215	24	-1.4239419472281867
2215	25	-1.2579035679910788
2216	1	-0.5640557738580938
2216	2	-1.4831613078021313
2216	3	-0.7847942426706176
2216	4	-0.5612657326414947
2216	5	-0.5058917597154249
2216	6	-0.4606179869784768
2216	7	-0.37048386021434254
2216	8	-0.5938510430961585
2216	9	-0.47163734736266605
2216	10	-0.8571383054394275
2216	11	-0.8978710633495837
2216	12	-0.7579131815940556
2216	13	-0.8844064495749109
2216	14	-0.8213854037926822
2216	15	-0.6985155945347188
2216	16	-0.8235975772704691
2216	17	-0.6203960141417397
2216	18	-0.6005048724915554
2216	19	-0.8016939017500491
2216	20	-0.7242524704616855
2216	21	-1.0600020595002064
2216	22	-1.175374273569588
2216	23	-1.1849150182571293
2216	24	-1.1184395974069972
2216	25	-1.0961093142103877
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	6580.0
3	0.0
4	102.0
5	905.0
6	1497.0
7	1121.0
8	877.0
9	600.0
10	611.0
11	571.0
12	491.0
13	465.0
14	456.0
15	430.0
16	426.0
17	470.0
18	641.0
19	822.0
20	1096.0
21	1359.0
22	1568.0
23	1883.0
24	2358.0
25	2619.0
26	3078.0
27	3511.0
28	3975.0
29	4889.0
30	6208.0
31	8118.0
32	11106.0
33	14625.0
34	20318.0
35	29616.0
36	45588.0
37	85361.0
38	221591.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	49.07660146457316	14.148263293310462	13.812219620002638	22.962915622113737
2	10.223982119299343	22.311154651184328	37.63694027623707	29.827922953279263
3	22.541425549253805	29.178568194726832	26.806014010190726	21.473992245828637
4	14.150498094399866	14.642718010343215	32.09900463568924	39.10777925956768
5	16.916215748770785	32.77205379718166	29.540887340531548	20.77084311351601
6	37.82163742690059	28.718186310847543	13.256939296598302	20.20323696565357
7	26.31201862797779	31.91605880742237	21.746022922624306	20.025899641975535
8	26.534829961939305	33.26327750137401	24.51096432092844	15.690928215758241
9	28.385301424534266	21.362044940443724	14.913048042268798	35.339605592753216
10	13.959141165913374	25.43180452303918	29.80624367112055	30.802810639926893
11	31.276861137183225	25.801923298588815	17.30515898499893	25.616056579229035
12	26.803709924418317	23.49145383589389	24.604184162426517	25.100652077261277
13	32.66252436296131	18.895161572483797	27.004966236588796	21.437347827966107
14	32.645366307452925	18.921288559220873	22.75662926343347	25.676715869892732
15	35.181015961343746	17.354771528057885	26.169112865386357	21.29509964521201
16	28.576220610176467	20.252096516952204	31.705715902659875	19.46596697021145
17	25.572713877661897	28.357877233851653	28.115868063844324	17.95354082464213
18	27.709432595507188	17.007317896331173	38.20575718413276	17.077492324028874
19	24.801618333429367	33.98047463431097	19.446753866796175	21.77115316546348
20	19.412221076654916	28.526478039063157	29.599881464652388	22.461419419629536
21	20.379498050030357	32.91939782467767	21.60792747553534	25.09317664975664
22	19.421719775504283	31.214125036788236	17.50296878505173	31.86118640265575
23	23.12116840595398	24.534343635978242	19.05080392733888	33.29368403072889
24	18.033235581622677	29.44960642074394	19.855944847455884	32.661213150177495
25	27.117187838029956	27.803086026756002	18.80255239013576	26.277173745078287
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	266.0
1	266.0
2	348.0
3	430.0
4	430.0
5	430.0
6	958.5
7	1487.0
8	1487.0
9	1487.0
10	1347.0
11	1207.0
12	1207.0
13	1207.0
14	1073.0
15	939.0
16	939.0
17	939.0
18	1479.0
19	2019.0
20	2019.0
21	2019.0
22	2911.5
23	3804.0
24	3804.0
25	3804.0
26	5457.0
27	7110.0
28	7110.0
29	7110.0
30	8306.5
31	9503.0
32	9503.0
33	9503.0
34	11584.5
35	13666.0
36	13666.0
37	13666.0
38	15710.0
39	17754.0
40	17754.0
41	17754.0
42	22835.0
43	27916.0
44	27916.0
45	27916.0
46	63201.5
47	98487.0
48	98487.0
49	98487.0
50	147030.0
51	195573.0
52	195573.0
53	195573.0
54	122157.5
55	48742.0
56	48742.0
57	48742.0
58	34160.5
59	19579.0
60	19579.0
61	19579.0
62	16908.5
63	14238.0
64	14238.0
65	14238.0
66	11925.5
67	9613.0
68	9613.0
69	9613.0
70	7426.5
71	5240.0
72	5240.0
73	5240.0
74	4422.0
75	3604.0
76	3604.0
77	3604.0
78	3129.0
79	2654.0
80	2654.0
81	2654.0
82	1958.0
83	1262.0
84	1262.0
85	1262.0
86	928.5
87	595.0
88	595.0
89	595.0
90	410.0
91	225.0
92	225.0
93	225.0
94	121.5
95	18.0
96	18.0
97	18.0
98	9.5
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.1802721368421919
2	0.009260554974770133
3	0.0
4	0.1611336565610003
5	0.05268226830091453
6	0.06009071228073064
7	0.043421713326144404
8	0.02654692426100771
9	0.01831531983898982
10	0.013170567075228632
11	0.02057901105504474
12	0.02057901105504474
13	0.010495295638072817
14	0.011524246190825054
15	0.001646320884403579
16	0.0014405307738531317
17	0.0
18	0.0
19	0.0
20	2.0579011055044737E-4
21	0.005556332984862079
22	0.008848974753669237
23	0.0010289505527522368
24	0.0014405307738531317
25	0.11997563445091082
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	485932.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.441792102130297
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.56232002951481	24.577505664344056
2	5.3428529185860985	2.932349180481996
3	1.3769569811997024	1.133585016349775
4	0.6772530424216668	0.7434014876268243
5	0.48991050686389515	0.6722011139004144
6	0.31019416575343944	0.5107370284739778
7	0.21601883406711467	0.41495607542300394
8	0.1949283915102315	0.4279347515701146
9	0.16180966572041983	0.39963124861334637
>10	1.247053702771689	7.033661188686002
>50	0.205458999846733	3.877734486414769
>100	0.1505194138071304	7.914494087579185
>500	0.02634089741624782	5.250926678584124
>1k	0.029351285692390424	16.13361420665932
>5k	0.005268179483249564	9.9822336315707
>10k+	0.0037629853451782596	17.995034153722393
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTACGGAAGCAGTGGTATCAACGCA	26698	5.4941843714758445	No Hit
CTTCCGTACTCTGCGTTGATACCAC	16524	3.400475786735593	No Hit
GAGTACGGAAGCAGTGGTATCAACG	16120	3.317336582073212	No Hit
TCCGTACTCTGCGTTGATACCACTG	14924	3.0712116098548767	No Hit
GCTTCCGTACTCTGCGTTGATACCA	12866	2.647695562342056	No Hit
CCCATGTACTCTGCGTTGATACCAC	9583	1.972086629404937	No Hit
GTACATGGGAAGCAGTGGTATCAAC	8781	1.8070429607434786	No Hit
GCGTTGATACCACTGCTTCCGTACT	7008	1.4421770947375352	No Hit
GTATCAACGCAGAGTACGGAAGCAG	6583	1.3547162977535951	No Hit
TCCATGTACTCTGCGTTGATACCAC	5678	1.1684762477054402	No Hit
GTACATGGAAGCAGTGGTATCAACG	5594	1.1511898784192027	No Hit
TATCAACGCAGAGTACGGAAGCAGT	5107	1.0509700945811349	No Hit
ACTCTGCGTTGATACCACTGCTTCC	4922	1.012898924129302	No Hit
CAGTGGTATCAACGCAGAGTACGGA	3882	0.7988772091568367	No Hit
CATGTACTCTGCGTTGATACCACTG	3861	0.7945556168352774	No Hit
ACGCAGAGTACGGAAGCAGTGGTAT	3521	0.7245869792481253	No Hit
GAGTACATGGGAAGCAGTGGTATCA	3488	0.7177959055999604	No Hit
GGTATCAACGCAGAGTACGGAAGCA	3304	0.6799305252586781	No Hit
ATACCACTGCTTCCGTACTCTGCGT	2982	0.6136661096614341	No Hit
AAGCAGTGGTATCAACGCAGAGTAC	2796	0.5753891490990509	No Hit
GCGTTGATACCACTGCTTCCCATGT	2406	0.4951310059843764	No Hit
GAAGCAGTGGTATCAACGCAGAGTA	2396	0.49307310487887196	No Hit
GTGGTATCAACGCAGAGTACGGAAG	2315	0.4764041059242857	No Hit
GTATCAACGCAGAGTACATGGGAAG	2229	0.45870615641694723	No Hit
GAGTACATGGAAGCAGTGGTATCAA	2172	0.4469761201155717	No Hit
GTACGGGAAGCAGTGGTATCAACGC	2068	0.4255739486183252	No Hit
GCTTCCCATGTACTCTGCGTTGATA	2043	0.420429195854564	No Hit
ATCAACGCAGAGTACGGAAGCAGTG	1802	0.3708337792119062	No Hit
GATACCACTGCTTCCGTACTCTGCG	1716	0.3531358297045677	No Hit
TATCAACGCAGAGTACATGGGAAGC	1701	0.350048978046311	No Hit
GTACTCTGCGTTGATACCACTGCTT	1695	0.3488142373830083	No Hit
CCCGTACTCTGCGTTGATACCACTG	1671	0.34387527472979756	No Hit
CGGAAGCAGTGGTATCAACGCAGAG	1635	0.33646683074998146	No Hit
CCACTGCTTCCGTACTCTGCGTTGA	1628	0.3350262999761283	No Hit
ACGCAGAGTACATGGGAAGCAGTGG	1605	0.33029312743346806	No Hit
GTTGATACCACTGCTTCCGTACTCT	1603	0.3298815472123672	No Hit
TTCCCGTACTCTGCGTTGATACCAC	1545	0.3179457208004412	No Hit
CAGTGGTATCAACGCAGAGTACATG	1500	0.30868516582567107	No Hit
CTGCTTCCGTACTCTGCGTTGATAC	1434	0.29510301852934157	No Hit
GCTTCCATGTACTCTGCGTTGATAC	1422	0.2926335372027362	No Hit
CATGGAAGCAGTGGTATCAACGCAG	1395	0.28707720421787414	No Hit
GAGTACGGGAAGCAGTGGTATCAAC	1314	0.27040820526328785	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	1234	0.25394499641925206	No Hit
GCGTTGATACCACTGCTTCCATGTA	1166	0.23995126890182164	No Hit
AAAAAGTACTCTGCGTTGATACCAC	1150	0.2366586271330145	No Hit
GCAGTGGTATCAACGCAGAGTACGG	1140	0.23460072602751003	No Hit
CCATGTACTCTGCGTTGATACCACT	1118	0.2300733435954002	No Hit
GGTATCAACGCAGAGTACATGGGAA	1111	0.22863281282154707	No Hit
GTATCAACGCAGAGTACTTTTTTTT	1068	0.21978383806787782	No Hit
GCAGAGTACGGAAGCAGTGGTATCA	1057	0.2175201468518229	No Hit
CTTCCCGTACTCTGCGTTGATACCA	1024	0.2107290732036581	No Hit
GTATCAACGCAGAGTACATGGAAGC	949	0.19529481491237455	No Hit
CACTGCTTCCGTACTCTGCGTTGAT	906	0.18644584015870533	No Hit
GTATCAACGCAGAGTACGGGAAGCA	899	0.1850053093848522	No Hit
ATACCACTGCTTCCCATGTACTCTG	897	0.1845937291637513	No Hit
GGGAAGCAGTGGTATCAACGCAGAG	895	0.18418214894265042	No Hit
CTGCGTTGATACCACTGCTTCCGTA	859	0.1767737049628343	No Hit
GCTTCCCGTACTCTGCGTTGATACC	843	0.17348106319402715	No Hit
CTGCTTCCCATGTACTCTGCGTTGA	838	0.1724521126412749	No Hit
GCGTTGATACCACTGCTTCCCGTAC	832	0.17121737197797224	No Hit
TGATACCACTGCTTCCGTACTCTGC	822	0.16915947087246774	No Hit
GCCCATGTACTCTGCGTTGATACCA	813	0.16730735987751372	No Hit
CATGGGAAGCAGTGGTATCAACGCA	800	0.16463208844035793	No Hit
CCCCATGTACTCTGCGTTGATACCA	788	0.16216260711375255	No Hit
TATCAACGCAGAGTACATGGAAGCA	787	0.16195681700320208	No Hit
AAAGTACTCTGCGTTGATACCACTG	783	0.1611336565610003	No Hit
TATCAACGCAGAGTACTTTTTTTTT	754	0.15516574335503733	No Hit
GCAGTGGTATCAACGCAGAGTACAT	725	0.14919783014907434	No Hit
ACGGAAGCAGTGGTATCAACGCAGA	716	0.14734571915412034	No Hit
ACGCAGAGTACATGGAAGCAGTGGT	715	0.1471399290435699	No Hit
TACCACTGCTTCCGTACTCTGCGTT	714	0.14693413893301943	No Hit
TACCACTGCTTCCCATGTACTCTGC	713	0.146728348822469	No Hit
TCAACGCAGAGTACGGAAGCAGTGG	710	0.14611097849081764	No Hit
GTGGTATCAACGCAGAGTACATGGG	705	0.1450820279380654	No Hit
TATCAACGCAGAGTACGGGAAGCAG	672	0.13829095428990065	No Hit
TACGGAAGCAGTGGTATCAACGCAG	670	0.13787937406879977	No Hit
TCCCATGTACTCTGCGTTGATACCA	662	0.13623305318439619	No Hit
CTTCCCATGTACTCTGCGTTGATAC	591	0.1216219553353144	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	585	0.12038721467201173	No Hit
GCAGAGTACATGGGAAGCAGTGGTA	584	0.12018142456146128	No Hit
ACATGGGAAGCAGTGGTATCAACGC	548	0.11277298058164516	No Hit
GATACCACTGCTTCCCATGTACTCT	540	0.11112665969724159	No Hit
CCACTGCTTCCCATGTACTCTGCGT	540	0.11112665969724159	No Hit
CTCTGCGTTGATACCACTGCTTCCG	538	0.11071507947614069	No Hit
GTTGATACCACTGCTTCCCATGTAC	529	0.10886296848118668	No Hit
GGTATCAACGCAGAGTACTTTTTTT	503	0.10351242560687503	No Hit
ATACCACTGCTTCCATGTACTCTGC	500	0.1028950552752237	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	2.057901105504474E-4	0.0
9	0.0	0.0	0.0	2.057901105504474E-4	0.0
10	0.0	0.0	0.0	8.231604422017896E-4	0.0
11	0.0	0.0	0.0	0.0014405307738531317	0.0
12	0.0	0.0	0.0	0.0016463208844035792	0.0
13	0.0	0.0	0.0	0.0026752714371558162	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTAGCAC	25	0.005977716	19.026606	3
AAACGCT	65	9.969881E-9	17.539507	19
CGTCTTG	45	3.5196583E-5	16.895115	5
TAAACGC	75	3.2923708E-9	16.455786	18
TACCCTA	35	0.00216198	16.29172	5
AGAACCG	35	0.00216198	16.29172	5
GAACCGC	35	0.0021694314	16.28333	6
AACCGCG	35	0.0021724182	16.279978	7
GTAAACG	70	2.5140253E-8	16.273277	17
AGTAAAC	70	2.5140253E-8	16.273277	16
GTTATCA	60	1.4159668E-6	15.881711	1
GGACGGA	55	1.09304565E-5	15.592952	1
TCAAAGT	80	7.727067E-9	15.428885	12
CCGTCTT	50	8.591097E-5	15.221285	4
TCCGTCT	50	8.591097E-5	15.221285	3
TTTCAAA	75	5.856964E-8	15.191519	10
GCGCCGA	45	6.747439E-4	14.778659	19
GCACTTA	45	6.779124E-4	14.769532	11
TGCACTG	40	0.005259308	14.255254	5
CGCGGTC	40	0.005291751	14.242048	10
>>END_MODULE