FastQCFastQC Report
Thu 2 Feb 2017
SRR4050221_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4050221_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3176596
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNN66680.20991023095162242No Hit
GNNNNNNNNNNNNNNNNNNNNNNNN51380.16174546590123515No Hit
GTCCTATTCCATTATTCCTAGCTGC35870.11291961584035239No Hit
GTATCTGATCGTCTTCGAACCTCCG34510.10863830339142906No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG34060.10722169265465298No Hit
GAATAGGACCGCGGTTCTATTTTGT32740.10306630116010974No Hit
CTATTGGAGCTGGAATTACCGCGGC32310.1017126509005237No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA13100.014.8689671
TCGACGC551.9622555E-413.81727114
CTCGACG551.9625192E-413.81705413
GTATCAA44200.013.4570641
AATCGTA1001.4457873E-712.34899113
CGTCGTA3950.012.26501510
CGAGCCG6550.012.182404515
AGTTTCG550.00307116512.09011316
TCGGCGT1103.823334E-812.08992213
AGGCCCG7000.012.07777610
CGCATCG4200.011.76094313
CCGTCGT4200.011.5351379
ATCGCCA4650.011.44010716
GCGTTAT2000.011.4019581
CGAACGA1751.8189894E-1211.39924916
AGACCGT1354.7293724E-911.2601236
CGTCTTA2700.011.25851715
AAGCTCG3750.011.1466397
GAGCCGC7600.011.12426716
ACCGTCG4450.011.1005688
Produced by FastQC (version 0.11.5)