##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4050221_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3176596
Sequences flagged as poor quality	0
Sequence length	25
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.891751736764764	31.0	30.0	34.0	26.0	34.0
2	30.02084275117138	31.0	31.0	34.0	26.0	34.0
3	30.13535589668941	31.0	31.0	34.0	26.0	34.0
4	33.56109338423898	37.0	35.0	37.0	28.0	37.0
5	33.618737163932714	37.0	35.0	37.0	28.0	37.0
6	33.7103049931436	37.0	35.0	37.0	29.0	37.0
7	33.645429887842205	37.0	35.0	37.0	29.0	37.0
8	33.565943544599314	37.0	35.0	37.0	28.0	37.0
9	35.082958613559924	39.0	35.0	39.0	28.0	39.0
10	34.97299341811171	39.0	35.0	39.0	28.0	39.0
11	35.00962949018383	39.0	35.0	39.0	28.0	39.0
12	34.9058199405905	39.0	35.0	39.0	27.0	39.0
13	34.90431644439519	39.0	35.0	39.0	27.0	39.0
14	35.77184004513007	40.0	36.0	41.0	27.0	41.0
15	35.881421496469805	40.0	36.0	41.0	27.0	41.0
16	35.90085109973065	40.0	36.0	41.0	27.0	41.0
17	35.883031395871555	40.0	36.0	41.0	27.0	41.0
18	35.715554637731714	40.0	36.0	41.0	27.0	41.0
19	35.855177365960294	40.0	36.0	41.0	27.0	41.0
20	35.81735417409076	40.0	36.0	41.0	27.0	41.0
21	35.782254337662074	40.0	36.0	41.0	27.0	41.0
22	35.69736031903333	40.0	36.0	41.0	27.0	41.0
23	35.58952035449267	40.0	36.0	41.0	27.0	41.0
24	35.520813474549485	40.0	36.0	41.0	27.0	41.0
25	35.23922148110745	40.0	36.0	41.0	25.0	41.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.9342526994586535
1101	2	1.0102050277787953
1101	3	0.90650280452979
1101	4	1.0662976949748142
1101	5	0.9808983810833709
1101	6	0.9214343163585497
1101	7	1.0438561877546988
1101	8	1.050973273758629
1101	9	1.231014063604782
1101	10	1.2315493043079115
1101	11	1.235714750312269
1101	12	1.1350631287863706
1101	13	1.2736272176117112
1101	14	1.5724934554961507
1101	15	1.5647394299822892
1101	16	1.594779594650987
1101	17	1.501934021929415
1101	18	1.6712901417625332
1101	19	1.5451339944559876
1101	20	1.5646135213566907
1101	21	1.5025437605431904
1101	22	1.5226805109715613
1101	23	1.537161273909021
1101	24	1.612200901923437
1101	25	1.6311642888546345
1102	1	0.8321292395522093
1102	2	0.8521125870180768
1102	3	0.7823379538102273
1102	4	0.8925147523097507
1102	5	0.7757496118801299
1102	6	0.787558808319389
1102	7	0.7815729830820146
1102	8	0.7030303530766417
1102	9	0.8960764661507312
1102	10	0.9413227475992372
1102	11	0.9354524231733947
1102	12	0.9409729981659893
1102	13	0.9881659244443597
1102	14	1.3693880261870817
1102	15	1.1737465808731145
1102	16	1.1226682286327403
1102	17	1.0975216698678665
1102	18	1.2248171796705591
1102	19	1.1348939699167389
1102	20	1.1131264683672484
1102	21	1.1629519332637202
1102	22	1.167225240784198
1102	23	1.2164775298840738
1102	24	1.1525372941424479
1102	25	1.1540512133964427
1103	1	0.6954821342761761
1103	2	0.6780269447449854
1103	3	0.6446215528207055
1103	4	0.7579233895434925
1103	5	0.6732868107572827
1103	6	0.6292332852722851
1103	7	0.6456298547069039
1103	8	0.7058856306386758
1103	9	0.7543222554763531
1103	10	0.841817375702199
1103	11	0.7774607803070808
1103	12	0.8614410955038139
1103	13	0.834147780627049
1103	14	1.153190002176757
1103	15	1.0722632508014982
1103	16	1.056794834774145
1103	17	1.0237172361225362
1103	18	1.0386704742098303
1103	19	1.0532055108951326
1103	20	1.012826339325521
1103	21	0.985837862058375
1103	22	0.9744898963467463
1103	23	0.939355642021944
1103	24	0.9208729922214971
1103	25	0.960360287445603
1104	1	0.7103032103456393
1104	2	0.6095388923096046
1104	3	0.621853694528884
1104	4	0.6819530118415429
1104	5	0.6220394593476612
1104	6	0.6204487708720094
1104	7	0.706097958932034
1104	8	0.6516907745385225
1104	9	0.6485156887002503
1104	10	0.7698308676574399
1104	11	0.7881069830474559
1104	12	0.8096179755663755
1104	13	0.8405212183585604
1104	14	1.2247885282681565
1104	15	1.0508675305338215
1104	16	0.9991250538345824
1104	17	0.9172828757568823
1104	18	0.9722683877658724
1104	19	0.9627180268749171
1104	20	0.9311086629743457
1104	21	0.9404309212901012
1104	22	0.9410329100427575
1104	23	1.002272049329278
1104	24	0.9451739353274107
1104	25	1.0379410522176897
1105	1	0.5749931660543517
1105	2	0.5856931775559069
1105	3	0.5346037930340302
1105	4	0.6747687265495443
1105	5	0.5653940789315044
1105	6	0.5994410763985982
1105	7	0.7132331234593536
1105	8	0.6380371351555851
1105	9	0.7616007398543871
1105	10	0.7753652576408072
1105	11	0.6898867188603433
1105	12	0.7857393964728061
1105	13	0.8101785575280402
1105	14	1.134638568461071
1105	15	0.9518152888870759
1105	16	0.9706672595555119
1105	17	1.0046560624625371
1105	18	1.1122911256711774
1105	19	1.0411178428346162
1105	20	0.9825034817969325
1105	21	0.9560405161307841
1105	22	0.9338112789099995
1105	23	0.947914694799671
1105	24	1.015776818461461
1105	25	0.9991734333287425
1106	1	0.36425704246429547
1106	2	0.26942861842049837
1106	3	0.26506911834196245
1106	4	0.423628194173304
1106	5	0.2969888638984841
1106	6	0.2638529467934845
1106	7	0.24299535460379218
1106	8	0.3501745844283022
1106	9	0.37365884811402594
1106	10	0.38985362542197777
1106	11	0.4259542864933934
1106	12	0.42899986920174626
1106	13	0.4657769700956962
1106	14	0.6748356772789705
1106	15	0.5151135287203488
1106	16	0.493533716975449
1106	17	0.4963419608024964
1106	18	0.6574181227239251
1106	19	0.5747308717290096
1106	20	0.5498720184648818
1106	21	0.609854839797741
1106	22	0.5917245907133832
1106	23	0.5160800420490119
1106	24	0.5127187082465667
1106	25	0.5061298625903987
1107	1	0.21514647119504104
1107	2	0.2619816955060017
1107	3	0.24696965794465697
1107	4	0.3133982589172817
1107	5	0.2453600930037112
1107	6	0.2339716382341166
1107	7	0.2229962478065346
1107	8	0.2428975063696086
1107	9	0.32063763942397827
1107	10	0.31598067727330914
1107	11	0.2663529209756206
1107	12	0.2780336919636355
1107	13	0.1851134584480576
1107	14	0.49809898976966593
1107	15	0.4206359385036649
1107	16	0.28169226137062253
1107	17	0.24816380312940822
1107	18	0.38211581057237254
1107	19	0.2572749676237791
1107	20	0.2606866833996264
1107	21	0.30055401717124397
1107	22	0.37233367152802543
1107	23	0.38952746709052377
1107	24	0.38869110423317466
1107	25	0.3850832917349436
1108	1	0.3027337183357375
1108	2	0.33324680474482093
1108	3	0.23934292151454173
1108	4	0.437263339373537
1108	5	0.25100080054075846
1108	6	0.3060419203674698
1108	7	0.24346575692251093
1108	8	0.262264339799529
1108	9	0.22935559954984797
1108	10	0.35493599555832844
1108	11	0.3378224554690803
1108	12	0.35358121113809204
1108	13	0.4059745276559852
1108	14	0.6946465038043783
1108	15	0.4790249068801131
1108	16	0.4704692064743128
1108	17	0.47301367444308795
1108	18	0.5845356016675112
1108	19	0.5347829534997643
1108	20	0.5638419074988548
1108	21	0.4243297142810363
1108	22	0.5376962755917631
1108	23	0.5200037028454076
1108	24	0.6232328848843522
1108	25	0.5711799812263507
1109	1	0.14987740095831725
1109	2	0.1705201759031887
1109	3	0.16496656829492196
1109	4	0.33758162166996186
1109	5	0.16616133429216262
1109	6	0.22608034613352856
1109	7	0.202023839188179
1109	8	0.16731676406333662
1109	9	0.10877561812629466
1109	10	0.23708947051289897
1109	11	0.18479229899340766
1109	12	0.20545462476770382
1109	13	0.09210831145339426
1109	14	0.29962491845481765
1109	15	0.12420922515988764
1109	16	0.19137600855325587
1109	17	0.08991656904129997
1109	18	0.2197819475019216
1109	19	0.11490631545813557
1109	20	0.16257621393204857
1109	21	0.1461923557153284
1109	22	0.1256392462853455
1109	23	0.0803670651566506
1109	24	0.04391255401191785
1109	25	0.006063651599063746
1110	1	-0.10195512164257536
1110	2	-0.12685239383064228
1110	3	-0.17859566949693573
1110	4	-0.1402561685879462
1110	5	-0.17543071326250015
1110	6	-0.1742637720878335
1110	7	-0.2762928392563211
1110	8	-0.2578801777142701
1110	9	-0.19005878709412372
1110	10	-0.21919190789227372
1110	11	-0.2197733755342952
1110	12	-0.1704866567354273
1110	13	-0.24692033343811204
1110	14	-0.04623887653566783
1110	15	-0.1578387341976537
1110	16	-0.28489741614048825
1110	17	-0.33077112178013834
1110	18	-0.08671779622940079
1110	19	-0.1211608794613781
1110	20	-0.3119739819829235
1110	21	-0.246947564417475
1110	22	-0.27037027254525725
1110	23	-0.23448528135778446
1110	24	-0.13911640830457372
1110	25	-0.40330887864504206
1111	1	-0.35769711845445684
1111	2	-0.39631730074393445
1111	3	-0.5095377763008813
1111	4	-0.31311921644149265
1111	5	-0.4440403816152454
1111	6	-0.34370579390280653
1111	7	-0.40223788163503116
1111	8	-0.33974055133190006
1111	9	-0.5140723271747234
1111	10	-0.4065379023987674
1111	11	-0.41849830760145323
1111	12	-0.43609222066822184
1111	13	-0.49216961139082116
1111	14	-0.21807347704531566
1111	15	-0.3492503427041669
1111	16	-0.4424048322585321
1111	17	-0.4094340074097502
1111	18	-0.34673462171267033
1111	19	-0.4732590480970913
1111	20	-0.5364603551184359
1111	21	-0.368422061174158
1111	22	-0.4940327821412964
1111	23	-0.4446492804596147
1111	24	-0.5389705180464262
1111	25	-0.5903312868358626
1112	1	-0.4931050637523029
1112	2	-0.4592697395662668
1112	3	-0.5061679154969454
1112	4	-0.45222542160919943
1112	5	-0.4590645076617008
1112	6	-0.5171847612285845
1112	7	-0.572798243815285
1112	8	-0.49531261918050973
1112	9	-0.6082304104719185
1112	10	-0.6078105581289464
1112	11	-0.6054139230382773
1112	12	-0.528086579272653
1112	13	-0.6563024847671741
1112	14	-0.5726475406574565
1112	15	-0.5557226911354007
1112	16	-0.6065306022773527
1112	17	-0.7800753408674197
1112	18	-0.6908915590248412
1112	19	-0.6735118296476799
1112	20	-0.7029784869634028
1112	21	-0.6627957996064922
1112	22	-0.6294446815248591
1112	23	-0.6232439599048547
1112	24	-0.6233436738634737
1112	25	-0.6450600321817248
1113	1	-0.6119720562328972
1113	2	-0.7704467061948002
1113	3	-0.7253001399714663
1113	4	-0.5469118033120282
1113	5	-0.5491194241624058
1113	6	-0.7468149826820039
1113	7	-0.7871879959323849
1113	8	-0.7432966437969526
1113	9	-0.7298975946207307
1113	10	-0.7507993847867667
1113	11	-0.7844173709439488
1113	12	-0.7538548404320409
1113	13	-0.8521662038114428
1113	14	-0.7759290320644325
1113	15	-0.8559095015008609
1113	16	-0.8710766739677211
1113	17	-0.9480757126828223
1113	18	-0.946850493405762
1113	19	-0.8727063835029583
1113	20	-1.0712412449654565
1113	21	-0.9742663047090332
1113	22	-0.9854489007614404
1113	23	-1.3242562473151338
1113	24	-1.2389678241694995
1113	25	-1.1971548770810898
1114	1	-2.4378381116103007
1114	2	-2.5861703321705534
1114	3	-2.2666276741918523
1114	4	-5.444850334596328
1114	5	-3.004250178138399
1114	6	-2.4791302891802616
1114	7	-2.302061815834481
1114	8	-2.442406093406955
1114	9	-3.044421511465245
1114	10	-2.856431549802764
1114	11	-2.508343083748123
1114	12	-2.6886853094883634
1114	13	-2.6504157754081277
1114	14	-2.537445492629878
1114	15	-3.1150709544168933
1114	16	-3.167309782370289
1114	17	-2.9414793531356054
1114	18	-2.7389270589628794
1114	19	-2.9919118707178214
1114	20	-3.169584051179399
1114	21	-3.0710580598856367
1114	22	-3.1705990985616097
1114	23	-3.334548787355935
1114	24	-3.388971448960973
1114	25	-3.3550064521083236
1115	1	-2.632332736739272
1115	2	-2.1019419598224296
1115	3	-1.6886081318072783
1115	4	-1.697322413053385
1115	5	-1.5944100870964846
1115	6	-1.6294426450025483
1115	7	-1.7681216562038813
1115	8	-1.6980888313055047
1115	9	-2.0954315367732192
1115	10	-1.8998256446924984
1115	11	-1.8706594180383718
1115	12	-2.1958762691147626
1115	13	-2.3377650703921233
1115	14	-2.556858881426116
1115	15	-2.486552648327006
1115	16	-2.3212829263021675
1115	17	-2.4845131689152637
1115	18	-2.348206671985416
1115	19	-2.1933633885657855
1115	20	-2.2610093942655354
1115	21	-2.233810738864719
1115	22	-2.198026292760048
1115	23	-2.3399556166411415
1115	24	-2.4943351011291597
1115	25	-2.714009099463894
1116	1	-1.1332565936795689
1116	2	-1.1209389872607218
1116	3	-1.285360586525318
1116	4	-1.140991445569739
1116	5	-1.3304964346503922
1116	6	-1.3377474948892356
1116	7	-1.4344901084875943
1116	8	-1.3069084515682832
1116	9	-1.6283568819809133
1116	10	-1.9669196740712849
1116	11	-1.7797407237592964
1116	12	-1.7289115750334076
1116	13	-1.6183519684507104
1116	14	-2.429137003264451
1116	15	-2.4517501417369587
1116	16	-2.394856340910607
1116	17	-2.0085179076259436
1116	18	-2.074721062348509
1116	19	-1.8913287765331148
1116	20	-2.034568622656856
1116	21	-2.2647236044081254
1116	22	-2.091467586243901
1116	23	-2.4186928610233878
1116	24	-2.412610805000412
1116	25	-2.286348874087672
1201	1	0.9874776607244016
1201	2	0.9383163012388849
1201	3	0.8955038443145433
1201	4	1.0858643661216547
1201	5	0.9988300298719537
1201	6	0.9450866365792692
1201	7	1.0201151230184067
1201	8	1.0425919160025856
1201	9	1.2117710368004353
1201	10	1.1782959983943329
1201	11	1.1239178934141947
1201	12	1.2537853561850696
1201	13	1.3037185141762961
1201	14	1.5917017472229773
1201	15	1.4232496476609384
1201	16	1.4644872881511404
1201	17	1.3755841982640433
1201	18	1.4699756303747833
1201	19	1.4428789767318264
1201	20	1.4455321930378489
1201	21	1.4573848614781681
1201	22	1.4640292503253107
1201	23	1.450675053736866
1201	24	1.4362151678362238
1201	25	1.4100783033555544
1202	1	0.8015394508198135
1202	2	0.7840570747408755
1202	3	0.6976809520652445
1202	4	0.8003375741968242
1202	5	0.7265940590943885
1202	6	0.7101820477560068
1202	7	0.7138014200756615
1202	8	0.7342406235490344
1202	9	0.929954847055626
1202	10	0.9130816750577964
1202	11	0.963389292175016
1202	12	1.0042037098698415
1202	13	1.013386384372069
1202	14	1.3334024222982634
1202	15	1.1614156261180355
1202	16	1.2114917672093313
1202	17	1.150071760233061
1202	18	1.2656443328551532
1202	19	1.1301700511831
1202	20	1.137104406794272
1202	21	1.2111138583540892
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2211	7	-0.06831476081254806
2211	8	-0.07685346050823227
2211	9	-0.08087334433296434
2211	10	-0.059131526208744845
2211	11	-0.013682370296905333
2211	12	-0.04832632991257668
2211	13	-0.057176658576700845
2211	14	0.18432966378022542
2211	15	-0.025712597746000654
2211	16	-0.03810431950009274
2211	17	-0.13006950793288752
2211	18	-0.03922021749531268
2211	19	-0.03927258907020814
2211	20	-0.0959525470094178
2211	21	-0.12180236854099036
2211	22	-0.15364887624212287
2211	23	-0.052257394955489644
2211	24	-0.073008642025016
2211	25	-0.08246661298078095
2212	1	-0.30617654096969815
2212	2	-0.36850618415533276
2212	3	-0.3665590494539934
2212	4	-0.3184754142916475
2212	5	-0.2653632718037571
2212	6	-0.3763126340876255
2212	7	-0.3843870545274797
2212	8	-0.43655876776522007
2212	9	-0.37792660949666157
2212	10	-0.45836731407140263
2212	11	-0.5062954000225446
2212	12	-0.44704478947044635
2212	13	-0.39416699122468657
2212	14	-0.46052607776861265
2212	15	-0.5220628741965427
2212	16	-0.4183465083241771
2212	17	-0.668261925387192
2212	18	-0.4292776988936069
2212	19	-0.5613104538642375
2212	20	-0.5222371689520955
2212	21	-0.676289682459732
2212	22	-0.7659961779033395
2212	23	-0.9140598345338233
2212	24	-0.6335094115441819
2212	25	-0.6819630586981518
2213	1	-0.8759805789919994
2213	2	-0.6719714492940767
2213	3	-0.6495089795225795
2213	4	-0.5943151379691258
2213	5	-0.5726628303529182
2213	6	-0.7042627072281604
2213	7	-0.7627293621051905
2213	8	-0.6831720392163732
2213	9	-0.5860847492317447
2213	10	-0.8113738224021034
2213	11	-0.7881209201846886
2213	12	-0.7889794761357578
2213	13	-0.9435627112978011
2213	14	-0.7638878026259306
2213	15	-0.8875410840956448
2213	16	-1.0053596156267801
2213	17	-1.0243980412908371
2213	18	-0.9166909313207086
2213	19	-0.949046556041317
2213	20	-0.960851637270494
2213	21	-0.8930805713878129
2213	22	-1.041217615205852
2213	23	-0.9051362477135072
2213	24	-0.9785405367332487
2213	25	-1.0839010073343118
2214	1	-1.1410380971860938
2214	2	-1.0357142354380784
2214	3	-0.9527794994321219
2214	4	-0.8861132565112655
2214	5	-0.8642086755103904
2214	6	-1.1734220881376416
2214	7	-1.0631652208832847
2214	8	-1.0462727030741448
2214	9	-1.0234336707415252
2214	10	-1.1747031251408941
2214	11	-1.2528745475133576
2214	12	-1.1454688434301161
2214	13	-1.3642230586417483
2214	14	-1.1337885294540015
2214	15	-1.2826040598991426
2214	16	-1.2036230678908666
2214	17	-1.3349856246377243
2214	18	-1.167460136367474
2214	19	-1.4034873519880975
2214	20	-1.3809313051572047
2214	21	-1.4277310587058238
2214	22	-1.3774822118340282
2214	23	-1.4291102694377074
2214	24	-1.3874693632316308
2214	25	-1.3551641136470565
2215	1	-1.6956092056657859
2215	2	-1.7668281803874386
2215	3	-1.647194969371622
2215	4	-1.911450616356916
2215	5	-1.7223159125247562
2215	6	-1.5115799858801822
2215	7	-1.610833298314077
2215	8	-1.771068298371965
2215	9	-2.1964467890775268
2215	10	-1.8176128432802727
2215	11	-2.043514919447965
2215	12	-2.149577068131663
2215	13	-2.2116599761554525
2215	14	-2.8214268563421925
2215	15	-2.4886439497259047
2215	16	-2.3159668803976317
2215	17	-2.2676445047849256
2215	18	-2.1697544959855435
2215	19	-2.3750979845162163
2215	20	-2.5984464195692993
2215	21	-2.407149281262953
2215	22	-2.7614267200334837
2215	23	-2.3229153735446175
2215	24	-2.3978758069267556
2215	25	-2.391237645943626
2216	1	-1.5642532445484782
2216	2	-1.4117188008705703
2216	3	-1.3415829781783586
2216	4	-1.3135334887793064
2216	5	-1.800235168460759
2216	6	-1.5105841224133485
2216	7	-1.5778697757973177
2216	8	-2.022545556984827
2216	9	-2.0028855701971295
2216	10	-2.079762028502792
2216	11	-2.003353001703964
2216	12	-2.0113463043046167
2216	13	-2.0334771327356975
2216	14	-1.8845413601698269
2216	15	-1.9073701824491138
2216	16	-1.9999446291916243
2216	17	-1.9975008409879251
2216	18	-2.4521902972456218
2216	19	-2.153519073124201
2216	20	-2.3261015851199716
2216	21	-2.449023588762543
2216	22	-2.6924407317393744
2216	23	-2.3262957005000686
2216	24	-2.519548526227638
2216	25	-2.547958661348865
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	120390.0
3	11.0
4	923.0
5	5141.0
6	6369.0
7	5666.0
8	5272.0
9	4890.0
10	4601.0
11	4416.0
12	4198.0
13	4091.0
14	4252.0
15	4345.0
16	4427.0
17	4848.0
18	5456.0
19	6484.0
20	8041.0
21	10292.0
22	12878.0
23	15393.0
24	18117.0
25	20595.0
26	23801.0
27	27142.0
28	30769.0
29	37707.0
30	46664.0
31	60372.0
32	78346.0
33	102728.0
34	140968.0
35	196614.0
36	289191.0
37	507697.0
38	1353501.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.34963216423215	18.721119631567973	12.478685274612534	25.450562929587345
2	15.658573328155557	20.743680398545237	39.51579338135659	24.081952891942624
3	17.750752484711963	25.97789755312211	29.458790633620158	26.81255932854577
4	11.89386608298186	17.25137038697099	38.92975984770135	31.925003682345803
5	13.931769177008944	37.187648702883166	34.29478954601703	14.585792574090863
6	30.481764471406997	38.07886103279423	18.6554612795637	12.783913216235074
7	27.745173179999664	31.814824754203435	22.640341908397115	17.79966015739978
8	26.04729425749701	33.64486662332511	21.077747404892527	19.23009171428535
9	27.137292321651014	15.304235871662986	19.983437687308097	37.575034119377904
10	15.093413117500434	27.67826933239052	32.878939945180065	24.34937760492898
11	34.83911704705701	22.828521307593583	23.707792798230116	18.624568847119292
12	23.727104869298316	25.095268329094257	30.002521901894212	21.17510489971321
13	28.36228398685491	21.155831701496265	25.852354359927553	24.629529951721278
14	22.718202521692824	21.2700712677112	27.066429413857367	28.945296796738607
15	24.058145672117664	28.033674155374044	23.465176148413143	24.44300402409515
16	24.242036364005042	27.40654820763735	24.767406161673353	23.584009266684262
17	23.00651594388038	27.052699747612518	26.127331796069903	23.8134525124372
18	23.274578154884427	26.51200042072835	27.38878182352157	22.824639600865662
19	24.826223280076974	26.218654690506206	26.525102384196636	22.430019645220188
20	24.81437700216997	25.818887716264456	25.96545344922144	23.401281832344136
21	26.137989132483774	25.565592725081494	25.24497216589009	23.051445976544642
22	24.73026061500833	25.754535258745783	25.880090483401872	23.635113642844015
23	23.537203960396038	25.487366336633666	26.188198019801977	24.78723168316832
24	23.583022216335916	26.5480730813114	26.138136897393334	23.730767804959346
25	23.8400655410926	25.916959106197922	26.10355860127046	24.13941675143902
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	11081.0
1	11081.0
2	10128.0
3	9175.0
4	9175.0
5	9175.0
6	6311.0
7	3447.0
8	3447.0
9	3447.0
10	5372.0
11	7297.0
12	7297.0
13	7297.0
14	12384.5
15	17472.0
16	17472.0
17	17472.0
18	28257.5
19	39043.0
20	39043.0
21	39043.0
22	58680.0
23	78317.0
24	78317.0
25	78317.0
26	107556.5
27	136796.0
28	136796.0
29	136796.0
30	162492.5
31	188189.0
32	188189.0
33	188189.0
34	224166.5
35	260144.0
36	260144.0
37	260144.0
38	283257.0
39	306370.0
40	306370.0
41	306370.0
42	331919.0
43	357468.0
44	357468.0
45	357468.0
46	380144.5
47	402821.0
48	402821.0
49	402821.0
50	395900.0
51	388979.0
52	388979.0
53	388979.0
54	349078.0
55	309177.0
56	309177.0
57	309177.0
58	273428.0
59	237679.0
60	237679.0
61	237679.0
62	206991.0
63	176303.0
64	176303.0
65	176303.0
66	144713.5
67	113124.0
68	113124.0
69	113124.0
70	85837.0
71	58550.0
72	58550.0
73	58550.0
74	46445.5
75	34341.0
76	34341.0
77	34341.0
78	28524.0
79	22707.0
80	22707.0
81	22707.0
82	16567.0
83	10427.0
84	10427.0
85	10427.0
86	7822.5
87	5218.0
88	5218.0
89	5218.0
90	3714.5
91	2211.0
92	2211.0
93	2211.0
94	1197.0
95	183.0
96	183.0
97	183.0
98	130.0
99	77.0
100	77.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.2152933517513716
2	0.6143053759433054
3	0.6103703461189273
4	0.6183033662448735
5	0.6338231238722205
6	0.6489336383978321
7	0.6431097942577526
8	0.6381673967983339
9	0.630612139535528
10	0.6321231909880891
11	0.6369396674931279
12	0.6374118710720532
13	0.6337916436336254
14	0.62667710971115
15	0.6330675981459399
16	0.6404024937385806
17	0.6402450925456055
18	0.6347990112686662
19	0.6328472364757748
20	0.6400876913526303
21	0.6364359836756075
22	0.6364045034370125
23	0.6404969344543656
24	0.6451874900050243
25	0.6328472364757748
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	3176596.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	67.94618982641619
#Duplication Level	Percentage of deduplicated	Percentage of total
1	92.22357631314134	62.662406226436815
2	5.343191616418996	7.260990236962413
3	0.9983148412455307	2.0349506912939215
4	0.3896666072476007	1.059054450602442
5	0.22349559625914323	0.759283710439591
6	0.14925758135709813	0.6084890373552688
7	0.09622609056961579	0.4576737351267922
8	0.07167159764906739	0.3895849583220846
9	0.054549335987128675	0.3335777584097761
>10	0.33285821728856163	4.391356117326447
>50	0.04892875199028022	2.3570257680363187
>100	0.05544118485949846	8.243509246587797
>500	0.008282996641026968	3.881239855827546
>1k	0.004445676162614239	5.185468720254997
>5k	9.359318237082608E-5	0.37538948701784064
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
NNNNNNNNNNNNNNNNNNNNNNNNN	6668	0.20991023095162242	No Hit
GNNNNNNNNNNNNNNNNNNNNNNNN	5138	0.16174546590123515	No Hit
GTCCTATTCCATTATTCCTAGCTGC	3587	0.11291961584035239	No Hit
GTATCTGATCGTCTTCGAACCTCCG	3451	0.10863830339142906	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG	3406	0.10722169265465298	No Hit
GAATAGGACCGCGGTTCTATTTTGT	3274	0.10306630116010974	No Hit
CTATTGGAGCTGGAATTACCGCGGC	3231	0.1017126509005237	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	9.444071578507308E-5	0.0
5	0.0	0.0	0.0	1.2592095438009743E-4	0.0
6	0.0	0.0	0.0	1.2592095438009743E-4	0.0
7	0.0	0.0	0.0	1.2592095438009743E-4	0.0
8	3.1480238595024357E-5	0.0	0.0	1.2592095438009743E-4	0.0
9	3.1480238595024357E-5	0.0	0.0	1.574011929751218E-4	0.0
10	3.1480238595024357E-5	0.0	0.0	1.574011929751218E-4	0.0
11	3.1480238595024357E-5	0.0	0.0	1.574011929751218E-4	0.0
12	3.1480238595024357E-5	0.0	0.0	1.574011929751218E-4	3.1480238595024357E-5
13	3.1480238595024357E-5	0.0	0.0	1.574011929751218E-4	6.296047719004871E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1310	0.0	14.868967	1
TCGACGC	55	1.9622555E-4	13.817271	14
CTCGACG	55	1.9625192E-4	13.817054	13
GTATCAA	4420	0.0	13.457064	1
AATCGTA	100	1.4457873E-7	12.348991	13
CGTCGTA	395	0.0	12.265015	10
CGAGCCG	655	0.0	12.1824045	15
AGTTTCG	55	0.003071165	12.090113	16
TCGGCGT	110	3.823334E-8	12.089922	13
AGGCCCG	700	0.0	12.077776	10
CGCATCG	420	0.0	11.760943	13
CCGTCGT	420	0.0	11.535137	9
ATCGCCA	465	0.0	11.440107	16
GCGTTAT	200	0.0	11.401958	1
CGAACGA	175	1.8189894E-12	11.399249	16
AGACCGT	135	4.7293724E-9	11.260123	6
CGTCTTA	270	0.0	11.258517	15
AAGCTCG	375	0.0	11.146639	7
GAGCCGC	760	0.0	11.124267	16
ACCGTCG	445	0.0	11.100568	8
>>END_MODULE