FastQCFastQC Report
Thu 2 Feb 2017
SRR4050221_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4050221_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3176596
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCAAAGCAGGCCCGAGCCGCCTG34550.10876422434580915No Hit
CTATTGGAGCTGGAATTACCGCGGC33740.10621432501961219No Hit
GAATAGGACCGCGGTTCTATTTTGT33600.10577360167928185No Hit
GTATCAACGCAGAGTACTTTTTTTT32900.10356998497763013No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA12200.016.1672131
GTATCAA42000.013.6802061
CGCATCG4800.013.45641513
GCATCGC5050.012.977530514
TCGACGC1102.759407E-912.95188414
AACCGCG3900.012.6642637
GAGCGTT1352.7284841E-1112.6642637
ATCGCCA5300.012.54301216
CCGCATC5250.012.30300812
TAGACCG550.003067143312.0922335
CATCGCC5650.011.59938615
CGACGCT1406.8394E-1011.53334315
CGAACGA1751.8189894E-1211.39622216
ACGCTTA1354.7621143E-911.25517417
CGCCAGT5750.011.23035218
GAACCGC4700.011.1135126
CTCGACG1303.2772732E-810.95997613
GGAGCGT2000.010.9215526
TCGCCAG6000.010.92103517
CTCGTAG3850.010.85559610
Produced by FastQC (version 0.11.5)