##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR4050221_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3176596 Sequences flagged as poor quality 0 Sequence length 25 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.728197416353858 31.0 31.0 34.0 27.0 34.0 2 30.70992282304706 31.0 31.0 34.0 27.0 34.0 3 30.909782673024836 31.0 31.0 34.0 27.0 34.0 4 34.54823213276098 37.0 35.0 37.0 31.0 37.0 5 34.49280046943332 37.0 35.0 37.0 31.0 37.0 6 34.43831006523965 37.0 35.0 37.0 31.0 37.0 7 34.39465767758947 37.0 35.0 37.0 30.0 37.0 8 34.36005239570912 37.0 35.0 37.0 30.0 37.0 9 35.95686609187948 39.0 37.0 39.0 31.0 39.0 10 35.83545342246858 39.0 37.0 39.0 30.0 39.0 11 35.81890205742248 39.0 37.0 39.0 30.0 39.0 12 35.75137001998365 39.0 37.0 39.0 30.0 39.0 13 35.75566046170177 39.0 37.0 39.0 30.0 39.0 14 36.90635132701798 40.0 37.0 41.0 31.0 41.0 15 36.87072199297613 40.0 37.0 41.0 31.0 41.0 16 36.852769127707774 40.0 37.0 41.0 31.0 41.0 17 36.82271179589724 40.0 37.0 41.0 31.0 41.0 18 36.78512313180524 40.0 37.0 41.0 31.0 41.0 19 36.8445565630631 40.0 37.0 41.0 31.0 41.0 20 36.79720933980903 40.0 37.0 41.0 31.0 41.0 21 36.74376817196773 40.0 37.0 41.0 30.0 41.0 22 36.68392329399143 40.0 37.0 41.0 30.0 41.0 23 36.58634210960412 40.0 37.0 41.0 30.0 41.0 24 36.52971136398837 40.0 37.0 41.0 30.0 41.0 25 36.308211998000374 40.0 37.0 41.0 30.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.9552917829066097 1101 2 1.173224186522102 1101 3 1.0260038257042368 1101 4 0.9369255973028601 1101 5 1.0176472912705279 1101 6 1.1568858378410596 1101 7 1.1554649871311895 1101 8 1.1315000951958964 1101 9 1.3085709256195202 1101 10 1.3107416129854244 1101 11 1.3483127901691887 1101 12 1.3211448520456983 1101 13 1.2285150957164959 1101 14 1.5394116555283048 1101 15 1.5380588974722258 1101 16 1.4784595042382165 1101 17 1.554347000655433 1101 18 1.6253971071473785 1101 19 1.5400246741828738 1101 20 1.4677525505996059 1101 21 1.5126950962990833 1101 22 1.5603838135223853 1101 23 1.5876476068374643 1101 24 1.5998384185066286 1101 25 1.5928097600306188 1102 1 0.9092315626924048 1102 2 1.0156150220068803 1102 3 0.9436986858816354 1102 4 0.8201201251779153 1102 5 0.9068452284398276 1102 6 0.9892373082789376 1102 7 0.9961459832713686 1102 8 1.0219082199882052 1102 9 1.0930888316035166 1102 10 1.1383921235807577 1102 11 1.1127639481150524 1102 12 1.163455008355612 1102 13 1.2040022062198048 1102 14 1.327255780167242 1102 15 1.3092587485752105 1102 16 1.316085874985383 1102 17 1.3187745938917956 1102 18 1.3296955497996592 1102 19 1.3347908323843498 1102 20 1.312963961989908 1102 21 1.3032934961354883 1102 22 1.3916652863886227 1102 23 1.4522947113337707 1102 24 1.4366623909255338 1102 25 1.4402626146355644 1103 1 0.5768484914687093 1103 2 0.7417970572273234 1103 3 0.682521344477589 1103 4 0.5844548990185245 1103 5 0.5642489975590976 1103 6 0.650530007483276 1103 7 0.6100235878043421 1103 8 0.6949577252263239 1103 9 0.7611809710664375 1103 10 0.8125983602408411 1103 11 0.7820786585662063 1103 12 0.8921400937922428 1103 13 0.8007473809153964 1103 14 0.8824501683810269 1103 15 0.9262159056514108 1103 16 0.8844907953892687 1103 17 0.8774049665532999 1103 18 0.9527638417703344 1103 19 0.9339332744225999 1103 20 0.9460526728263545 1103 21 0.8994043558224902 1103 22 0.8227460720142261 1103 23 0.8416407610362384 1103 24 0.930376862404863 1103 25 0.955938260455774 1104 1 0.6975869863009478 1104 2 0.8483941108072521 1104 3 0.7293610885363435 1104 4 0.718943819886384 1104 5 0.740400433505954 1104 6 0.882263144285659 1104 7 0.7603754647325331 1104 8 0.7210624489180972 1104 9 0.8334258579955716 1104 10 0.8798049048833079 1104 11 0.9338062582842781 1104 12 0.9441159799003742 1104 13 0.9457379189721848 1104 14 1.0210519185555782 1104 15 1.0934658167676048 1104 16 1.1070815846370294 1104 17 0.9198346215002928 1104 18 1.069881079787379 1104 19 1.0488966197333411 1104 20 1.0642722722664715 1104 21 1.0335575955499294 1104 22 1.0624078833761317 1104 23 1.1141552958825542 1104 24 1.0808908810449083 1104 25 1.1056991106224956 1105 1 0.5766412068378912 1105 2 0.7848060946569788 1105 3 0.6791889286040558 1105 4 0.644616018658347 1105 5 0.7142893639878594 1105 6 0.8099180996481579 1105 7 0.7536298392925147 1105 8 0.8043841830726493 1105 9 0.8573253507599645 1105 10 0.9027098559400457 1105 11 0.9256317370403835 1105 12 1.0079649775731525 1105 13 0.9275077005807404 1105 14 1.034816628862572 1105 15 1.0600691448883381 1105 16 1.0329876123084532 1105 17 1.0329248571358534 1105 18 1.0274405633638182 1105 19 1.0183033463431528 1105 20 1.004536589431602 1105 21 1.042745463230716 1105 22 1.0932578325330695 1105 23 1.0358384316256348 1105 24 1.0975958074270338 1105 25 1.195037567277943 1106 1 0.22084186164565622 1106 2 0.4506804563392741 1106 3 0.2977638728770451 1106 4 0.25381141509435423 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24 -1.105707623599855 2213 25 -1.1148523763631601 2214 1 -0.9168572170317297 2214 2 -0.8987106198055876 2214 3 -0.985468869301755 2214 4 -0.9221767598991661 2214 5 -1.017412838425173 2214 6 -1.0142971462324795 2214 7 -1.098479097345269 2214 8 -1.0727766729737027 2214 9 -1.187779539628302 2214 10 -1.1314847540067774 2214 11 -1.2432033735401404 2214 12 -1.3584034782360206 2214 13 -1.2292647700841073 2214 14 -1.4386334993800531 2214 15 -1.5445302984840978 2214 16 -1.5055426591440622 2214 17 -1.5207960184825922 2214 18 -1.5908700799463205 2214 19 -1.428113356040143 2214 20 -1.4698427843225303 2214 21 -1.6230098928840064 2214 22 -1.5643531358137182 2214 23 -1.6841232703376505 2214 24 -1.76504401627691 2214 25 -1.7782471607820796 2215 1 -1.5817127074616728 2215 2 -3.4933842118944973 2215 3 -1.8875317192147065 2215 4 -1.6120156104128966 2215 5 -1.4743739200097323 2215 6 -1.3798378071633834 2215 7 -1.5077756350719795 2215 8 -1.517285586286519 2215 9 -1.668685467778701 2215 10 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-3.0029931628102275 2216 22 -2.9756815974034225 2216 23 -2.950361553484278 2216 24 -3.08634001983571 2216 25 -3.2217447347556174 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 46471.0 3 6.0 4 914.0 5 7463.0 6 8628.0 7 6679.0 8 5378.0 9 4674.0 10 4376.0 11 3979.0 12 3680.0 13 3583.0 14 3485.0 15 3772.0 16 3774.0 17 4080.0 18 4382.0 19 5188.0 20 6759.0 21 8622.0 22 11080.0 23 13702.0 24 16606.0 25 18959.0 26 22250.0 27 25232.0 28 29691.0 29 36684.0 30 47011.0 31 61093.0 32 79834.0 33 105960.0 34 143033.0 35 197787.0 36 286682.0 37 496315.0 38 1448784.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.260464847491356 19.44520321485673 13.016058178409958 26.278273759241955 2 15.438924472273087 21.488453100875745 38.20337352310765 24.869248903743518 3 17.41452170814293 26.167413168057884 29.596240755827935 26.821824367971246 4 12.293347973742963 18.016612751511367 37.79353748477931 31.896501789966365 5 13.728503743381529 37.54413889627249 34.261389189482 14.465968170863983 6 30.453868326178746 38.491070090627595 18.278831273004766 12.776230310188899 7 27.64841400213947 32.00605952615563 22.435483738949266 17.91004273275563 8 26.030862683754606 34.02573264953864 20.78025881236686 19.163145854339895 9 26.926302719060246 15.189725965725096 19.83425463067615 38.04971668453852 10 14.966426323198315 28.003944399333907 32.7401516742445 24.289477603223276 11 34.87150152420068 22.75716774140943 23.64565254143109 18.7256781929588 12 23.656423461512187 25.308210735893173 29.811256244260253 21.224109558334387 13 28.362100994326234 21.086894918797743 25.807960907047185 24.74304317982884 14 22.770980259318 21.43082009490991 26.686218670568675 29.111980975203416 15 23.997930716144758 28.196511436792072 23.346635272662468 24.458922574400706 16 24.2571269625534 27.487128823218836 24.631212017317097 23.624532196910668 17 22.97342733427737 27.17658421960306 26.071535834974068 23.778452611145497 18 23.309971796792095 26.600104703504297 27.253415534713493 22.83650796499012 19 24.81310850316171 26.223145267566668 26.461588154293388 22.50215807497823 20 24.799398573500966 25.81915397324307 25.972438396598967 23.409009056656995 21 26.084436666518705 25.534525144096776 25.27272847473218 23.108309714652332 22 24.62852496369538 25.844765130440067 25.830441608352096 23.69626829751246 23 23.503823080526466 25.441839057880127 26.225280938493235 24.829056923100172 24 23.589744881286908 26.558973102948478 26.13432092484705 23.71696109091756 25 23.756218709694277 25.877765580577133 26.22601017534062 24.140005534387974 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 232.0 1 232.0 2 490.0 3 748.0 4 748.0 5 748.0 6 2102.0 7 3456.0 8 3456.0 9 3456.0 10 5356.5 11 7257.0 12 7257.0 13 7257.0 14 12184.5 15 17112.0 16 17112.0 17 17112.0 18 28358.5 19 39605.0 20 39605.0 21 39605.0 22 59669.5 23 79734.0 24 79734.0 25 79734.0 26 109443.0 27 139152.0 28 139152.0 29 139152.0 30 164261.0 31 189370.0 32 189370.0 33 189370.0 34 225351.0 35 261332.0 36 261332.0 37 261332.0 38 284989.5 39 308647.0 40 308647.0 41 308647.0 42 334617.5 43 360588.0 44 360588.0 45 360588.0 46 382644.0 47 404700.0 48 404700.0 49 404700.0 50 397894.0 51 391088.0 52 391088.0 53 391088.0 54 350295.5 55 309503.0 56 309503.0 57 309503.0 58 273659.0 59 237815.0 60 237815.0 61 237815.0 62 208071.0 63 178327.0 64 178327.0 65 178327.0 66 146347.0 67 114367.0 68 114367.0 69 114367.0 70 86263.0 71 58159.0 72 58159.0 73 58159.0 74 46332.5 75 34506.0 76 34506.0 77 34506.0 78 28837.0 79 23168.0 80 23168.0 81 23168.0 82 16719.0 83 10270.0 84 10270.0 85 10270.0 86 7722.5 87 5175.0 88 5175.0 89 5175.0 90 3634.5 91 2094.0 92 2094.0 93 2094.0 94 1120.5 95 147.0 96 147.0 97 147.0 98 95.5 99 44.0 100 44.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3123784075784267 2 0.010451439213548087 3 0.0 4 0.0 5 0.043820492124273906 6 0.0025184190876019487 7 0.0034628262454526795 8 2.8332214735521925E-4 9 0.008751506329416773 10 0.014071666651975888 11 0.005572002231319312 12 0.008782986568011797 13 0.018132617430734032 14 0.0034628262454526795 15 0.019454787451725054 16 0.009853314680242625 17 0.005477561515534238 18 2.2036167016517054E-4 19 0.004847956743633751 20 0.004659075312063605 21 0.0026758202805770705 22 9.444071578507308E-5 23 0.0031480238595024356 24 0.003588747199832777 25 0.002927662189337265 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 3176596.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 67.51851497976179 #Duplication Level Percentage of deduplicated Percentage of total 1 91.94391302553578 62.07916468912552 2 5.5499382828152335 7.494471821700274 3 1.0028694725170186 2.0313677250865836 4 0.4345121448148023 1.1735045903426662 5 0.22916308855153805 0.7736375713587749 6 0.14082489559262096 0.5704972693556261 7 0.09989264217786639 0.4721222000077979 8 0.07097429730258054 0.38336633244818796 9 0.06169260446398145 0.3748853734777665 >10 0.3466252579376299 4.5028352282422555 >50 0.0482456231193458 2.29263460414119 >100 0.05801489269946287 8.492648583181358 >500 0.008795611315494549 4.118401793124162 >1k 0.004538161156696014 5.240462218407853 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTTCAAAGCAGGCCCGAGCCGCCTG 3455 0.10876422434580915 No Hit CTATTGGAGCTGGAATTACCGCGGC 3374 0.10621432501961219 No Hit GAATAGGACCGCGGTTCTATTTTGT 3360 0.10577360167928185 No Hit GTATCAACGCAGAGTACTTTTTTTT 3290 0.10356998497763013 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 3.1480238595024357E-5 0.0 3 0.0 0.0 0.0 3.1480238595024357E-5 0.0 4 0.0 0.0 0.0 6.296047719004871E-5 0.0 5 0.0 0.0 0.0 6.296047719004871E-5 0.0 6 0.0 0.0 0.0 6.296047719004871E-5 6.296047719004871E-5 7 0.0 0.0 0.0 6.296047719004871E-5 6.296047719004871E-5 8 0.0 0.0 0.0 6.296047719004871E-5 6.296047719004871E-5 9 0.0 0.0 0.0 9.444071578507308E-5 6.296047719004871E-5 10 0.0 0.0 0.0 9.444071578507308E-5 6.296047719004871E-5 11 0.0 0.0 0.0 9.444071578507308E-5 6.296047719004871E-5 12 0.0 0.0 0.0 9.444071578507308E-5 1.2592095438009743E-4 13 0.0 0.0 0.0 9.444071578507308E-5 1.2592095438009743E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1220 0.0 16.167213 1 GTATCAA 4200 0.0 13.680206 1 CGCATCG 480 0.0 13.456415 13 GCATCGC 505 0.0 12.9775305 14 TCGACGC 110 2.759407E-9 12.951884 14 AACCGCG 390 0.0 12.664263 7 GAGCGTT 135 2.7284841E-11 12.664263 7 ATCGCCA 530 0.0 12.543012 16 CCGCATC 525 0.0 12.303008 12 TAGACCG 55 0.0030671433 12.092233 5 CATCGCC 565 0.0 11.599386 15 CGACGCT 140 6.8394E-10 11.533343 15 CGAACGA 175 1.8189894E-12 11.396222 16 ACGCTTA 135 4.7621143E-9 11.255174 17 CGCCAGT 575 0.0 11.230352 18 GAACCGC 470 0.0 11.113512 6 CTCGACG 130 3.2772732E-8 10.959976 13 GGAGCGT 200 0.0 10.921552 6 TCGCCAG 600 0.0 10.921035 17 CTCGTAG 385 0.0 10.855596 10 >>END_MODULE