Basic Statistics
Measure | Value |
---|---|
Filename | SRR4050220_2.fastq |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 3148483 |
Sequences flagged as poor quality | 0 |
Sequence length | 25 |
%GC | 47 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
NNNNNNNNNNNNNNNNNNNNNNNNN | 9951 | 0.31605697092853924 | No Hit |
GNNNNNNNNNNNNNNNNNNNNNNNN | 4442 | 0.14108381719069152 | No Hit |
GTCCTATTCCATTATTCCTAGCTGC | 3584 | 0.113832598111535 | No Hit |
GAATAGGACCGCGGTTCTATTTTGT | 3522 | 0.11186339580045374 | No Hit |
GTTCAAAGCAGGCCCGAGCCGCCTG | 3464 | 0.11002123879976484 | No Hit |
GTATCTGATCGTCTTCGAACCTCCG | 3354 | 0.10652749276397554 | No Hit |
CTATTGGAGCTGGAATTACCGCGGC | 3308 | 0.10506647169446365 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
GGTATCA | 1250 | 0.0 | 15.359591 | 1 |
GTATCAA | 4280 | 0.0 | 13.479793 | 1 |
GCGTTAT | 215 | 0.0 | 12.820291 | 1 |
ATCGCCA | 405 | 0.0 | 12.666241 | 16 |
CGCATCG | 400 | 0.0 | 12.587076 | 13 |
TCGCGTA | 160 | 0.0 | 12.46833 | 9 |
CGCGTAA | 165 | 0.0 | 12.090502 | 10 |
CGAACGT | 215 | 0.0 | 11.930021 | 4 |
CATCGCC | 440 | 0.0 | 11.658511 | 15 |
GTCGCGT | 180 | 0.0 | 11.61072 | 8 |
CCGCATC | 450 | 0.0 | 11.610534 | 12 |
CGAGCCG | 610 | 0.0 | 11.524018 | 15 |
CAAGACG | 340 | 0.0 | 11.45568 | 4 |
GCATCGC | 440 | 0.0 | 11.442796 | 14 |
GCCGCCT | 635 | 0.0 | 11.220274 | 18 |
TCGAACG | 230 | 0.0 | 11.151977 | 3 |
TCGCCAG | 460 | 0.0 | 11.151798 | 17 |
AATTCCG | 215 | 0.0 | 11.046316 | 5 |
ACGGTAT | 485 | 0.0 | 10.968702 | 9 |
CGGTTCT | 590 | 0.0 | 10.948609 | 12 |