FastQCFastQC Report
Thu 2 Feb 2017
SRR4050220_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4050220_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3148483
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNN99510.31605697092853924No Hit
GNNNNNNNNNNNNNNNNNNNNNNNN44420.14108381719069152No Hit
GTCCTATTCCATTATTCCTAGCTGC35840.113832598111535No Hit
GAATAGGACCGCGGTTCTATTTTGT35220.11186339580045374No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG34640.11002123879976484No Hit
GTATCTGATCGTCTTCGAACCTCCG33540.10652749276397554No Hit
CTATTGGAGCTGGAATTACCGCGGC33080.10506647169446365No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA12500.015.3595911
GTATCAA42800.013.4797931
GCGTTAT2150.012.8202911
ATCGCCA4050.012.66624116
CGCATCG4000.012.58707613
TCGCGTA1600.012.468339
CGCGTAA1650.012.09050210
CGAACGT2150.011.9300214
CATCGCC4400.011.65851115
GTCGCGT1800.011.610728
CCGCATC4500.011.61053412
CGAGCCG6100.011.52401815
CAAGACG3400.011.455684
GCATCGC4400.011.44279614
GCCGCCT6350.011.22027418
TCGAACG2300.011.1519773
TCGCCAG4600.011.15179817
AATTCCG2150.011.0463165
ACGGTAT4850.010.9687029
CGGTTCT5900.010.94860912
Produced by FastQC (version 0.11.5)