##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4050220_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3148483
Sequences flagged as poor quality	0
Sequence length	25
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.98113281856691	31.0	31.0	34.0	26.0	34.0
2	30.05195009787253	31.0	31.0	34.0	26.0	34.0
3	30.19808523660442	31.0	31.0	34.0	26.0	34.0
4	33.67552659487124	37.0	35.0	37.0	28.0	37.0
5	33.6835682454058	37.0	35.0	37.0	28.0	37.0
6	33.75758484324038	37.0	35.0	37.0	30.0	37.0
7	33.71238498032227	37.0	35.0	37.0	29.0	37.0
8	33.65141593586499	37.0	35.0	37.0	29.0	37.0
9	35.19477253013594	39.0	35.0	39.0	29.0	39.0
10	35.070469492768424	39.0	35.0	39.0	28.0	39.0
11	35.11112145118776	39.0	35.0	39.0	29.0	39.0
12	35.03529858665269	39.0	35.0	39.0	28.0	39.0
13	35.04226860999408	39.0	35.0	39.0	28.0	39.0
14	36.11164519547985	40.0	36.0	41.0	27.0	41.0
15	36.08799253481756	40.0	36.0	41.0	27.0	41.0
16	36.0845804153937	40.0	36.0	41.0	27.0	41.0
17	36.04343139219745	40.0	36.0	41.0	27.0	41.0
18	35.98312870039317	40.0	36.0	41.0	27.0	41.0
19	36.034267931572124	40.0	37.0	41.0	27.0	41.0
20	36.00257425560182	40.0	37.0	41.0	27.0	41.0
21	35.92550539418507	40.0	37.0	41.0	27.0	41.0
22	35.82962461604525	40.0	36.0	41.0	27.0	41.0
23	35.7534469774809	40.0	36.0	41.0	27.0	41.0
24	35.69139963595166	40.0	36.0	41.0	27.0	41.0
25	35.35812929591807	40.0	36.0	41.0	26.0	41.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	1.2287940414235052
1101	2	1.2514495361050813
1101	3	1.2981458653531384
1101	4	1.3781131648818459
1101	5	1.2957861286319954
1101	6	1.2473250145335086
1101	7	1.2617327072740068
1101	8	1.315191901761537
1101	9	1.5475923533865057
1101	10	1.573330313553754
1101	11	1.494954594972839
1101	12	1.5661535168953904
1101	13	1.57228990862545
1101	14	1.770565384994086
1101	15	1.7968965672360824
1101	16	1.8434722234327623
1101	17	1.8459695727588894
1101	18	1.8680874999907573
1101	19	1.7660006215263735
1101	20	1.8571179568680378
1101	21	1.9086548046027545
1101	22	1.9151272613143249
1101	23	1.9461308765278744
1101	24	1.9412058424129484
1101	25	1.9171220488920468
1102	1	1.059445067229312
1102	2	1.0419727438557445
1102	3	1.0649752270680963
1102	4	1.186599671887862
1102	5	1.1546900682896393
1102	6	1.114271679595916
1102	7	1.0716548045446928
1102	8	1.1017279460364762
1102	9	1.212099338889459
1102	10	1.3008119104835316
1102	11	1.2140510078781332
1102	12	1.1978031290295235
1102	13	1.2114392557155753
1102	14	1.466534104515965
1102	15	1.4413528570490328
1102	16	1.4009334531685482
1102	17	1.4577262719987587
1102	18	1.4648899655684602
1102	19	1.4275678725956453
1102	20	1.4550695164615846
1102	21	1.6114546641993854
1102	22	1.6517473579613409
1102	23	1.5821786646617966
1102	24	1.5786156924742087
1102	25	1.513565267138766
1103	1	0.8756720591421541
1103	2	0.9392582865232875
1103	3	0.9591420528379402
1103	4	0.9874593593856531
1103	5	0.991873011014853
1103	6	0.9587413513876726
1103	7	0.9587032363373567
1103	8	0.941755299997439
1103	9	1.0786475227954426
1103	10	1.152492393591487
1103	11	1.195298243214907
1103	12	1.162984282727912
1103	13	1.1778665212073207
1103	14	1.3752355947431028
1103	15	1.3404158375504522
1103	16	1.348759691285899
1103	17	1.4585704027415005
1103	18	1.4745329930387001
1103	19	1.4200818717109485
1103	20	1.3172240956081467
1103	21	1.366237286046882
1103	22	1.4385607884693954
1103	23	1.4022729440972626
1103	24	1.42595138210595
1103	25	1.4139530271153191
1104	1	0.8811996778291444
1104	2	0.9366590533145995
1104	3	0.9360465352538085
1104	4	1.0571207878894668
1104	5	1.0284375492834172
1104	6	0.9644254996339896
1104	7	0.8700080595493631
1104	8	0.9705395571091913
1104	9	1.1446762064703577
1104	10	1.1563471765706197
1104	11	1.1853440613623007
1104	12	1.161551992293866
1104	13	1.155948028283568
1104	14	1.2344582008868983
1104	15	1.24950733184685
1104	16	1.2492379891985266
1104	17	1.2990608178501333
1104	18	1.309122381025638
1104	19	1.3108470063727609
1104	20	1.2907349584850394
1104	21	1.3754693114172625
1104	22	1.449619291806357
1104	23	1.4284427268397195
1104	24	1.3437579329650404
1104	25	1.431417267187264
1105	1	0.6605681182764087
1105	2	0.753602454321058
1105	3	0.7867685380405725
1105	4	0.8536236955691976
1105	5	0.7358873356697231
1105	6	0.7579267145259152
1105	7	0.6717183049460189
1105	8	0.7024054238280613
1105	9	0.9083973916521373
1105	10	0.8525887270260597
1105	11	0.7789145078206943
1105	12	0.7459993028340648
1105	13	0.8934646279680436
1105	14	0.996084060235809
1105	15	0.9161118298258657
1105	16	0.9513308276697217
1105	17	0.9020846665816933
1105	18	0.9069662069563833
1105	19	0.9363638824307472
1105	20	0.9602676799581076
1105	21	0.9723561940391292
1105	22	1.0046711003255737
1105	23	1.1226268146343799
1105	24	0.967616245474396
1105	25	0.9468441606337592
1106	1	0.7963019960282374
1106	2	0.8233558096242568
1106	3	0.8192414025777062
1106	4	0.8652702972002686
1106	5	0.922817626631236
1106	6	0.8122323536343927
1106	7	0.7693352229410451
1106	8	0.835806809473219
1106	9	0.9523997662584378
1106	10	0.9641063252975073
1106	11	0.9994926317689377
1106	12	1.0081538298634456
1106	13	0.988395840860413
1106	14	1.1305699304825012
1106	15	1.1830395340242106
1106	16	1.1769229197866835
1106	17	1.1723430926807907
1106	18	1.2116165128746061
1106	19	1.192343217223808
1106	20	1.170792052800195
1106	21	1.2402127893865469
1106	22	1.2266813712555376
1106	23	1.205011345085758
1106	24	1.1613322964103716
1106	25	1.213631337336821
1107	1	0.4971076232033198
1107	2	0.6047705920298796
1107	3	0.5907237283134243
1107	4	0.6403902444900851
1107	5	0.6855785780257477
1107	6	0.6204854472952377
1107	7	0.5407557999667532
1107	8	0.47122677809746705
1107	9	0.5687484691317195
1107	10	0.6240684617288395
1107	11	0.6297077057226943
1107	12	0.5675234478828983
1107	13	0.6064962217170375
1107	14	0.8398723147566685
1107	15	0.7406191124639179
1107	16	0.7068343650699234
1107	17	0.6909277718668037
1107	18	0.7240855459806639
1107	19	0.6453987183776846
1107	20	0.7839983015677419
1107	21	0.7618946627246359
1107	22	0.76604020091726
1107	23	0.7574085747054866
1107	24	0.7274289905156692
1107	25	0.5934802935985459
1108	1	0.5642352052920891
1108	2	0.6588345110303777
1108	3	0.6395319319408159
1108	4	0.7957862194500578
1108	5	0.7091958317594447
1108	6	0.6305946365128037
1108	7	0.5483312940742096
1108	8	0.6886138814335112
1108	9	0.720979170724938
1108	10	0.7316828348094973
1108	11	0.7042331197755516
1108	12	0.60077169586841
1108	13	0.6134061799836559
1108	14	0.7377659602591748
1108	15	0.851143338821565
1108	16	0.6954247808288656
1108	17	0.8206216286609447
1108	18	0.8246127907902405
1108	19	0.8783698921617713
1108	20	0.8132031363403129
1108	21	0.8439348737537955
1108	22	0.7065717765491684
1108	23	0.7405469116697105
1108	24	0.700191891116738
1108	25	0.7224867963938948
1109	1	0.6340149770377685
1109	2	0.7013706683861649
1109	3	0.6095975021921411
1109	4	0.7092138630862337
1109	5	0.7700889360694916
1109	6	0.5824292578618255
1109	7	0.6168374682190603
1109	8	0.6858054785729664
1109	9	0.7321626392678766
1109	10	0.8381876344301418
1109	11	0.766614170565667
1109	12	0.7649786370554352
1109	13	0.7057407244362182
1109	14	0.7945405382898585
1109	15	0.7976206799983387
1109	16	0.8169230282304056
1109	17	0.806917317973614
1109	18	0.7200068531020065
1109	19	0.8831699133083006
1109	20	0.833936023674184
1109	21	0.8499526624679561
1109	22	0.867813237838206
1109	23	0.7457388644552907
1109	24	0.6720901603056078
1109	25	0.8129827945740118
1110	1	-0.06379366333811021
1110	2	0.09548500970577223
1110	3	-5.3738933424085644E-5
1110	4	0.017671251396450316
1110	5	0.12113267919593795
1110	6	-0.024346924964518735
1110	7	-0.1083316714860203
1110	8	-0.015474282817692142
1110	9	-0.010098046043019338
1110	10	0.03187139644266068
1110	11	0.026595399657118435
1110	12	-0.06305174187508555
1110	13	-0.089323678205524
1110	14	-0.014163290169371123
1110	15	-0.14931131288484067
1110	16	-0.10867818784808492
1110	17	-0.030109047667558286
1110	18	0.03644970835296135
1110	19	-0.032879328657926976
1110	20	-0.03429024062712216
1110	21	-0.008293581910848502
1110	22	0.019085801794609836
1110	23	0.04206748202969379
1110	24	0.03464081584792211
1110	25	0.19181105227670514
1111	1	-0.03185671291508285
1111	2	0.01798699640688639
1111	3	-0.11121482250793235
1111	4	-0.029680097157786633
1111	5	0.0035350905890680906
1111	6	-0.03439660173287251
1111	7	-0.088777357849672
1111	8	-0.06244292805386209
1111	9	-0.06836264875823872
1111	10	-0.06522957232824211
1111	11	0.028198214673992084
1111	12	-0.07923731269589496
1111	13	0.008967610987930641
1111	14	-0.13395578616825077
1111	15	-0.06621449862937823
1111	16	0.012198141393568562
1111	17	-0.0011505595563505722
1111	18	-0.04652062234498544
1111	19	-0.07537251843130832
1111	20	-0.03895213181873203
1111	21	0.08370583409143251
1111	22	0.01845475159667842
1111	23	-0.005362521407761278
1111	24	-0.04238545296105656
1111	25	-0.05456805095413131
1112	1	-0.0835767497568547
1112	2	0.055235137781057375
1112	3	-0.09164302810876279
1112	4	-0.029418887216039025
1112	5	-0.03516732362489705
1112	6	-0.05561927899282182
1112	7	-0.10191034519932884
1112	8	-0.18865467196637553
1112	9	-0.15624141938680225
1112	10	-0.1621113309103066
1112	11	-0.1270779451233679
1112	12	-0.2288251846570546
1112	13	-0.22538256280855506
1112	14	-0.3533838826733984
1112	15	-0.3510277309990073
1112	16	-0.199129240340298
1112	17	-0.15633142276116985
1112	18	-0.1407077391680147
1112	19	-0.192038354030025
1112	20	-0.1536102177328118
1112	21	-0.06697508438181643
1112	22	-0.08886574889454124
1112	23	-0.07588524249333517
1112	24	-0.16773502392102557
1112	25	-0.23041739399491945
1113	1	-0.492501791107653
1113	2	-0.3652574232514034
1113	3	-0.43449351178037077
1113	4	-0.38409617865785606
1113	5	-0.40503048107225936
1113	6	-0.4459015137964357
1113	7	-0.5189818336417602
1113	8	-0.5529095487882145
1113	9	-0.4972737278127184
1113	10	-0.5301397127782081
1113	11	-0.4933364436256369
1113	12	-0.5025639514866924
1113	13	-0.43411263271281086
1113	14	-0.6834343859425189
1113	15	-0.6962540628625646
1113	16	-0.6302838132075692
1113	17	-0.7631181235283648
1113	18	-0.7538713897154778
1113	19	-0.7402441966245377
1113	20	-0.8293287340504278
1113	21	-0.708028137960568
1113	22	-0.7006776479506129
1113	23	-0.8041243018994706
1113	24	-0.9525102927788751
1113	25	-0.9285169856793516
1114	1	-0.7696030121848132
1114	2	-0.6612762340854772
1114	3	-0.8380288358919863
1114	4	-0.7400643409012986
1114	5	-0.7450876378755709
1114	6	-0.854875604286832
1114	7	-0.8774219620215078
1114	8	-0.831521095092306
1114	9	-0.9965170431172581
1114	10	-0.8570419872410824
1114	11	-0.8714436559324596
1114	12	-0.9959305606816429
1114	13	-0.9934319996316674
1114	14	-1.1603455042266617
1114	15	-1.1004441971469348
1114	16	-1.0804765140371018
1114	17	-1.4662034887099509
1114	18	-1.2141919191900712
1114	19	-1.151738959875182
1114	20	-1.107132317241394
1114	21	-1.3381051080726465
1114	22	-1.1682748840596489
1114	23	-1.4150380497114767
1114	24	-1.2406643484713271
1114	25	-1.2586291736197381
1115	1	-1.153092512793144
1115	2	-1.0749212708482503
1115	3	-1.1820755808877266
1115	4	-1.2450391136102397
1115	5	-1.1192355024090475
1115	6	-1.1987050334382872
1115	7	-1.2290827908230426
1115	8	-1.3614094568786612
1115	9	-1.7619754294822485
1115	10	-1.4736401072030745
1115	11	-1.4318194531604362
1115	12	-1.4417907604663611
1115	13	-1.3954205202500276
1115	14	-1.5849736865644033
1115	15	-1.677789844343529
1115	16	-1.7810139131892981
1115	17	-1.6334158993450316
1115	18	-1.4299190577245398
1115	19	-1.5094985520310544
1115	20	-1.701334072457783
1115	21	-1.6503169752428093
1115	22	-1.7695523772488073
1115	23	-1.6074791188296942
1115	24	-1.8543410113863317
1115	25	-1.734801532643182
1116	1	-1.5256829286368294
1116	2	-1.6886563565643975
1116	3	-1.48898518358655
1116	4	-1.703578663277483
1116	5	-1.2510961935833862
1116	6	-1.3781594494652936
1116	7	-1.421011416931556
1116	8	-1.4415107653165862
1116	9	-1.8684811220329536
1116	10	-2.5379624176420847
1116	11	-1.9331774282338756
1116	12	-1.6375879874113153
1116	13	-1.6354368858998924
1116	14	-1.835013937379749
1116	15	-2.6664275065038154
1116	16	-2.041140083666427
1116	17	-1.8871877173220994
1116	18	-2.005997696662064
1116	19	-1.8126223168058928
1116	20	-1.8853757246648044
1116	21	-1.8355154238226206
1116	22	-1.9723558088494073
1116	23	-1.9261074373737443
1116	24	-1.915530089817537
1116	25	-2.0151135623907948
1201	1	1.0293442324065012
1201	2	1.022700794372284
1201	3	0.9967050342995485
1201	4	1.0312999419866173
1201	5	0.9553835567943452
1201	6	0.886645779139009
1201	7	0.8962219362962713
1201	8	0.9666178677660895
1201	9	1.0693396307146514
1201	10	1.1298274449939356
1201	11	1.1328148487784233
1201	12	1.1572141236735405
1201	13	1.0933578663676684
1201	14	1.334029462353378
1201	15	1.331241986775126
1201	16	1.2253741709383519
1201	17	1.2617805124878245
1201	18	1.3767281188819425
1201	19	1.3966698223550296
1201	20	1.3149868823907198
1201	21	1.3708839515699438
1201	22	1.4481003605744434
1201	23	1.4228848865392862
1201	24	1.467564126402209
1201	25	1.5052073305991982
1202	1	0.8493685273396636
1202	2	0.9506901241353063
1202	3	0.8601375990733224
1202	4	0.8975634565518789
1202	5	0.8786842086269999
1202	6	0.7617822346629026
1202	7	0.8562192300767464
1202	8	0.9188031400311019
1202	9	0.8882490852893312
1202	10	0.9985250310664711
1202	11	0.9950342946952304
1202	12	0.9935093091462264
1202	13	1.049745307474332
1202	14	1.1771779961622997
1202	15	1.1707742584429397
1202	16	1.1711502811242127
1202	17	1.2430953892683547
1202	18	1.1808365874160387
1202	19	1.1653080954207198
1202	20	1.1354390855687981
1202	21	1.2105998595625493
1202	22	1.2122923963535115
1202	23	1.1900064558210488
1202	24	1.1336373917002334
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2211	5	-0.39284903272694294
2211	6	-0.3751530739453486
2211	7	-0.571515920058367
2211	8	-0.36994595337247915
2211	9	-0.3225018313446313
2211	10	-0.505019853156
2211	11	-0.4396381732734085
2211	12	-0.3711474916695465
2211	13	-0.48983806053286827
2211	14	-0.5765605612482645
2211	15	-0.5100005955965372
2211	16	-0.5881641767015253
2211	17	-0.6795728584692924
2211	18	-0.6095749640335981
2211	19	-0.6796033056372934
2211	20	-0.5473141947788704
2211	21	-0.6005776614103482
2211	22	-0.543966529127907
2211	23	-0.6286748609747548
2211	24	-0.5906735649946953
2211	25	-0.6034910610410762
2212	1	-0.5446370572814878
2212	2	-0.5057260231105225
2212	3	-0.47503027786456187
2212	4	-0.479270067643057
2212	5	-0.44494861965847576
2212	6	-0.4888857246995215
2212	7	-0.494442098201894
2212	8	-0.5353212337912652
2212	9	-0.6941379125220735
2212	10	-0.6440651208798371
2212	11	-0.6932470616836781
2212	12	-0.6077501243297476
2212	13	-0.6029804476044802
2212	14	-0.7177107205846269
2212	15	-0.6921965042143725
2212	16	-0.9240582462133133
2212	17	-0.7435760479540932
2212	18	-0.7231767030981544
2212	19	-0.7654223041164272
2212	20	-0.8038560420356973
2212	21	-0.7456973331267918
2212	22	-0.645524175045459
2212	23	-0.7974116110644829
2212	24	-0.7293586804371799
2212	25	-0.8558374934290356
2213	1	-0.7499996291964486
2213	2	-0.5809393598150123
2213	3	-0.6746821027336978
2213	4	-0.6836629907687666
2213	5	-0.6011671785242285
2213	6	-0.6006430135976188
2213	7	-0.75033656195437
2213	8	-0.8280263139012121
2213	9	-0.6843464228344587
2213	10	-0.8526313524989391
2213	11	-0.8777546618631007
2213	12	-0.7933745329417832
2213	13	-0.8261426016978248
2213	14	-0.9286903631901637
2213	15	-0.9208274588512992
2213	16	-1.110917212466255
2213	17	-1.1419364502315261
2213	18	-0.9364251046356387
2213	19	-1.069449147926811
2213	20	-1.0195998527153165
2213	21	-1.0477779297552559
2213	22	-1.10879355077261
2213	23	-1.05123616768158
2213	24	-0.9716351813706154
2213	25	-1.075806662982238
2214	1	-1.14860666667251
2214	2	-1.1576341137925894
2214	3	-1.2101067452171677
2214	4	-1.1582301967865618
2214	5	-1.1641637982483957
2214	6	-1.2720306240985622
2214	7	-1.2763356476400176
2214	8	-1.3544671907353205
2214	9	-1.4324457792802292
2214	10	-1.847067385532469
2214	11	-1.7430392365766778
2214	12	-1.8024582135026606
2214	13	-1.6818112595514663
2214	14	-1.9181787141513738
2214	15	-1.8877565165125318
2214	16	-1.845841737220944
2214	17	-1.896507870114263
2214	18	-1.8571599895303308
2214	19	-1.829159003028387
2214	20	-1.907366926327981
2214	21	-1.8071586302609859
2214	22	-1.7356329044260548
2214	23	-1.863995552027049
2214	24	-1.9602414151677223
2214	25	-1.8721352259503021
2215	1	-1.764446547144889
2215	2	-1.5630483134342157
2215	3	-1.6762028384605259
2215	4	-2.2640271784642287
2215	5	-1.8888395439364025
2215	6	-1.7086425465583943
2215	7	-1.7144456767792775
2215	8	-1.8722423732763573
2215	9	-1.93599052808635
2215	10	-2.0462543020745727
2215	11	-2.0274082553854953
2215	12	-2.121465584438859
2215	13	-2.125558438243246
2215	14	-2.27240857847724
2215	15	-2.1916851975325713
2215	16	-2.257541374943308
2215	17	-2.212107271112984
2215	18	-2.5599063160965017
2215	19	-2.9056969150920366
2215	20	-2.5330550983389344
2215	21	-2.321134285397335
2215	22	-3.251675968789577
2215	23	-2.627364275339545
2215	24	-2.6736535825444463
2215	25	-2.4980885068610874
2216	1	-2.702216399240246
2216	2	-5.394524267020476
2216	3	-3.1726039761668936
2216	4	-5.72736335329968
2216	5	-3.3473399504500705
2216	6	-2.155634243729885
2216	7	-1.991275031305662
2216	8	-1.7879677565154175
2216	9	-2.0092346385660775
2216	10	-2.003547565586942
2216	11	-1.9919351468473607
2216	12	-2.136763545581566
2216	13	-1.9369675802874227
2216	14	-2.6278447464449
2216	15	-2.3743280260354283
2216	16	-2.474070563281714
2216	17	-2.250505369470673
2216	18	-2.1091891171031776
2216	19	-2.216539894748834
2216	20	-2.550530649244344
2216	21	-2.6995063659416374
2216	22	-2.6889800158259547
2216	23	-2.458768265759687
2216	24	-2.5386150141984416
2216	25	-2.8587643755308108
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	117410.0
3	15.0
4	856.0
5	4894.0
6	5928.0
7	5390.0
8	4742.0
9	4468.0
10	4331.0
11	4156.0
12	3922.0
13	3811.0
14	3937.0
15	3998.0
16	4302.0
17	4495.0
18	4999.0
19	6023.0
20	7631.0
21	9760.0
22	12052.0
23	14586.0
24	17057.0
25	19496.0
26	22175.0
27	25132.0
28	28925.0
29	35185.0
30	44228.0
31	57538.0
32	74263.0
33	99175.0
34	135613.0
35	189207.0
36	282813.0
37	505212.0
38	1380758.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.33557893689748	18.739397015589677	12.493005736650959	25.43201831086189
2	15.545357932106976	20.73702323463813	39.578494915184876	24.13912391807002
3	17.771633241762196	25.92295681297903	29.434690251111277	26.870719694147493
4	11.975775980587015	17.204400797063236	38.86108605142535	31.958737170924394
5	13.938088830793175	37.17794911536054	34.288714310341355	14.59524774350493
6	30.493493268103965	38.05907232374509	18.626959311816513	12.82047509633443
7	27.826402672991936	31.77264853642815	22.58591816921165	17.815030621368262
8	26.098265516933655	33.625724353866914	21.037088261381196	19.238921867818235
9	27.09972003093284	15.291831133302248	19.93207695855217	37.676371877212745
10	15.113831512093098	27.657171912925847	32.82210688233732	24.406889692643723
11	34.86574009662299	22.804369623726846	23.718242107603025	18.611648172047136
12	23.81237350023413	25.000671680172772	29.982331612979163	21.20462320661394
13	28.34388028767038	21.165906230459345	25.812707200875103	24.67750628099517
14	22.825302569102448	21.35938261700682	26.734454428787664	29.08086038510307
15	24.03971914861767	27.995449007485085	23.45158940886676	24.513242435030488
16	24.298928814851703	27.371561571156843	24.750034942813727	23.579474671177728
17	23.053969117094354	27.049075192118543	26.02957146170024	23.867384229086866
18	23.352588730874285	26.605907512036094	27.176852067174988	22.864651689914638
19	24.86189011731423	26.17821245026605	26.514122555207166	22.445774877212557
20	24.808587853832993	25.760011054357307	25.999170283482215	23.432230808327486
21	26.19740105046768	25.50501100780862	25.19305646047856	23.104531481245143
22	24.821826138137524	25.698172838242446	25.832906824986562	23.647094198633468
23	23.5605781707933	25.431339579546464	26.1730680595852	24.83501419007504
24	23.618544368174927	26.494335229053785	26.11712097852441	23.769999424246883
25	23.902960645214534	25.85557010604943	26.12595552922677	24.11551371950927
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	13813.0
1	13813.0
2	11034.0
3	8255.0
4	8255.0
5	8255.0
6	5882.0
7	3509.0
8	3509.0
9	3509.0
10	5363.5
11	7218.0
12	7218.0
13	7218.0
14	11994.5
15	16771.0
16	16771.0
17	16771.0
18	27520.5
19	38270.0
20	38270.0
21	38270.0
22	57696.0
23	77122.0
24	77122.0
25	77122.0
26	105840.5
27	134559.0
28	134559.0
29	134559.0
30	159632.5
31	184706.0
32	184706.0
33	184706.0
34	220897.5
35	257089.0
36	257089.0
37	257089.0
38	279764.0
39	302439.0
40	302439.0
41	302439.0
42	328072.5
43	353706.0
44	353706.0
45	353706.0
46	376422.5
47	399139.0
48	399139.0
49	399139.0
50	393029.0
51	386919.0
52	386919.0
53	386919.0
54	346178.0
55	305437.0
56	305437.0
57	305437.0
58	270236.0
59	235035.0
60	235035.0
61	235035.0
62	205975.0
63	176915.0
64	176915.0
65	176915.0
66	145230.0
67	113545.0
68	113545.0
69	113545.0
70	86041.5
71	58538.0
72	58538.0
73	58538.0
74	46662.0
75	34786.0
76	34786.0
77	34786.0
78	28795.5
79	22805.0
80	22805.0
81	22805.0
82	16545.5
83	10286.0
84	10286.0
85	10286.0
86	7774.0
87	5262.0
88	5262.0
89	5262.0
90	3681.0
91	2100.0
92	2100.0
93	2100.0
94	1141.0
95	182.0
96	182.0
97	182.0
98	129.5
99	77.0
100	77.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.38062774993544507
2	0.6586981730566752
3	0.6741024169417462
4	0.6829955886692098
5	0.6937309173973625
6	0.6947790412080992
7	0.7113902155418974
8	0.7011630680553143
9	0.6895066608268172
10	0.682646214065631
11	0.6955095517428552
12	0.6985904005198694
13	0.6983680712266828
14	0.7068483456953714
15	0.702941702400807
16	0.6970340954675633
17	0.7037674969183572
18	0.7070071523333619
19	0.7098339104895913
20	0.7023699985040415
21	0.7008454547793334
22	0.7090081159720412
23	0.6988127298130561
24	0.703291077004386
25	0.7052602793154672
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	3148483.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	67.63276568704619
#Duplication Level	Percentage of deduplicated	Percentage of total
1	92.15819348348255	62.32913506009843
2	5.386345216050071	7.285868478133134
3	1.002537228891148	2.034130964824069
4	0.4150970546023959	1.1229664732522746
5	0.22735787663945756	0.7688420998930392
6	0.14753015062580105	0.598672326542966
7	0.09449232455063732	0.4473544071890303
8	0.0692685983051104	0.3747861502911726
9	0.054505207360701184	0.3317704126335152
>10	0.3265885635846559	4.382617407928432
>50	0.049749547339431655	2.3959198077438297
>100	0.05510489473724787	8.207009879059095
>500	0.008504906034562698	3.9234137039379493
>1k	0.004677698318442645	5.479517391582398
>5k	4.7249477964067116E-5	0.31799543689063064
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
NNNNNNNNNNNNNNNNNNNNNNNNN	9951	0.31605697092853924	No Hit
GNNNNNNNNNNNNNNNNNNNNNNNN	4442	0.14108381719069152	No Hit
GTCCTATTCCATTATTCCTAGCTGC	3584	0.113832598111535	No Hit
GAATAGGACCGCGGTTCTATTTTGT	3522	0.11186339580045374	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG	3464	0.11002123879976484	No Hit
GTATCTGATCGTCTTCGAACCTCCG	3354	0.10652749276397554	No Hit
CTATTGGAGCTGGAATTACCGCGGC	3308	0.10506647169446365	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	3.176132759808454E-5	0.0
4	0.0	0.0	0.0	1.2704531039233816E-4	0.0
5	0.0	0.0	0.0	1.2704531039233816E-4	0.0
6	0.0	0.0	0.0	1.2704531039233816E-4	0.0
7	0.0	0.0	0.0	1.2704531039233816E-4	0.0
8	0.0	0.0	0.0	1.2704531039233816E-4	0.0
9	0.0	0.0	0.0	1.9056796558850723E-4	0.0
10	0.0	0.0	0.0	1.9056796558850723E-4	0.0
11	0.0	0.0	0.0	1.9056796558850723E-4	0.0
12	0.0	0.0	0.0	1.9056796558850723E-4	6.352265519616908E-5
13	0.0	0.0	0.0	1.9056796558850723E-4	6.352265519616908E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1250	0.0	15.359591	1
GTATCAA	4280	0.0	13.479793	1
GCGTTAT	215	0.0	12.820291	1
ATCGCCA	405	0.0	12.666241	16
CGCATCG	400	0.0	12.587076	13
TCGCGTA	160	0.0	12.46833	9
CGCGTAA	165	0.0	12.090502	10
CGAACGT	215	0.0	11.930021	4
CATCGCC	440	0.0	11.658511	15
GTCGCGT	180	0.0	11.61072	8
CCGCATC	450	0.0	11.610534	12
CGAGCCG	610	0.0	11.524018	15
CAAGACG	340	0.0	11.45568	4
GCATCGC	440	0.0	11.442796	14
GCCGCCT	635	0.0	11.220274	18
TCGAACG	230	0.0	11.151977	3
TCGCCAG	460	0.0	11.151798	17
AATTCCG	215	0.0	11.046316	5
ACGGTAT	485	0.0	10.968702	9
CGGTTCT	590	0.0	10.948609	12
>>END_MODULE