FastQCFastQC Report
Thu 2 Feb 2017
SRR4050220_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4050220_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3148483
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCAAAGCAGGCCCGAGCCGCCTG36070.11456310864629093No Hit
GAATAGGACCGCGGTTCTATTTTGT34630.10998947747216675No Hit
CTATTGGAGCTGGAATTACCGCGGC33890.1076391392299085No Hit
GTCCTATTCCATTATTCCTAGCTGC31820.101064544417105No Hit
ATACAGGACTCTTTCGAGGCCCTGT31550.10020698857195671No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA13050.015.3521171
GTATCAA43100.013.3696221
CTCGACG1001.0179065E-813.29121713
CCGACCA4200.011.9801799
CGTCTTA2650.011.82237615
CGCATCG3400.011.72754413
CGTCGTA3650.011.70459410
TCGACGC1157.147901E-811.5575814
ATACCGT4700.011.5175096
ACCGTCG3800.011.4924058
ATCGCCA3800.011.49240516
CCGTCGT3900.011.4411579
CGAACGA1501.7826096E-1011.39247216
CGACCAT4300.011.26000110
TATACCG855.263676E-511.1895275
TACACCG855.263676E-511.1895275
CATCGCC4000.011.155128515
GCATCGC3750.011.13930514
TCTCGTC2900.011.13057512
ACCGCTA600.005906911.0760148
Produced by FastQC (version 0.11.5)