##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4050220_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3148483
Sequences flagged as poor quality	0
Sequence length	25
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.77959639610568	31.0	31.0	34.0	27.0	34.0
2	30.862320361901272	31.0	31.0	34.0	27.0	34.0
3	30.999058276636717	31.0	31.0	34.0	27.0	34.0
4	34.60362720713436	37.0	35.0	37.0	31.0	37.0
5	34.52860822180079	37.0	35.0	37.0	32.0	37.0
6	34.544050579278974	37.0	35.0	37.0	31.0	37.0
7	34.46465456538911	37.0	35.0	37.0	31.0	37.0
8	34.429122215365304	37.0	35.0	37.0	31.0	37.0
9	36.04925927819842	39.0	37.0	39.0	31.0	39.0
10	35.9323467841497	39.0	37.0	39.0	31.0	39.0
11	35.91985664207175	39.0	37.0	39.0	31.0	39.0
12	35.835748835232714	39.0	37.0	39.0	30.0	39.0
13	35.83425700567543	39.0	37.0	39.0	30.0	39.0
14	37.010736916794535	40.0	37.0	41.0	31.0	41.0
15	36.97964225946273	40.0	37.0	41.0	31.0	41.0
16	36.94418486617206	40.0	37.0	41.0	31.0	41.0
17	36.92426638479547	40.0	37.0	41.0	31.0	41.0
18	36.89741789935026	40.0	37.0	41.0	31.0	41.0
19	36.942298243312734	40.0	37.0	41.0	31.0	41.0
20	36.912951411838655	40.0	37.0	41.0	31.0	41.0
21	36.84284368059157	40.0	37.0	41.0	31.0	41.0
22	36.77587428612446	40.0	37.0	41.0	31.0	41.0
23	36.68361239365117	40.0	37.0	41.0	30.0	41.0
24	36.6161649912037	40.0	37.0	41.0	30.0	41.0
25	36.33326049402204	40.0	37.0	41.0	30.0	41.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	1.0997545514761882
1101	2	1.2220498940698725
1101	3	1.150672109895936
1101	4	1.045025903609364
1101	5	1.0846324205229578
1101	6	1.054823282917333
1101	7	1.2192148496014852
1101	8	1.1746615360557655
1101	9	1.309521491815019
1101	10	1.2989127253544197
1101	11	1.3191642351869746
1101	12	1.3784746815420448
1101	13	1.375959349615833
1101	14	1.592152010445389
1101	15	1.5854930820730573
1101	16	1.5982559240729017
1101	17	1.5810340525983477
1101	18	1.642696482887665
1101	19	1.6788810317081158
1101	20	1.7200690324200636
1101	21	1.7371456764542756
1101	22	1.7983273353136937
1101	23	1.763897079729034
1101	24	1.7893287726784237
1101	25	1.8314560700571008
1102	1	0.9551835388028884
1102	2	1.020841075768189
1102	3	0.9834926102803649
1102	4	0.8674688041141749
1102	5	0.9453114554953999
1102	6	0.924276595843061
1102	7	1.054733513518535
1102	8	1.0578954273650325
1102	9	1.163492037575601
1102	10	1.221681855391381
1102	11	1.242258724230318
1102	12	1.2051556434854547
1102	13	1.2222894772380215
1102	14	1.418527822016351
1102	15	1.4829048845992645
1102	16	1.4796729037402159
1102	17	1.4312925832068117
1102	18	1.4043889653069286
1102	19	1.4599220375135928
1102	20	1.4312111715568179
1102	21	1.4692337537644207
1102	22	1.441940614189427
1102	23	1.4839052129269419
1102	24	1.435588916246779
1102	25	1.5158478850007313
1103	1	0.8145773831991932
1103	2	0.8711807708715007
1103	3	0.8658657657638429
1103	4	0.7903840025138749
1103	5	0.7468916250326032
1103	6	0.7468372691519889
1103	7	0.8166475915175866
1103	8	0.9062882692149046
1103	9	0.9946485055322256
1103	10	1.0556455122973674
1103	11	1.0201021729900575
1103	12	1.093621763151475
1103	13	1.0974777493303804
1103	14	1.273519910070732
1103	15	1.229791765776298
1103	16	1.2836767450015998
1103	17	1.235246631066616
1103	18	1.1876831710442275
1103	19	1.1947404516831241
1103	20	1.2431567166285902
1103	21	1.1986779179777542
1103	22	1.25415162585778
1103	23	1.281607586274923
1103	24	1.3280199460018238
1103	25	1.2927430002582199
1104	1	0.8501098298314673
1104	2	0.8371000211199977
1104	3	0.7790555702793931
1104	4	0.6962931708766291
1104	5	0.7092903091808438
1104	6	0.6701524582465126
1104	7	0.7727948911815261
1104	8	0.8217098151040503
1104	9	0.9021017643952902
1104	10	0.8876084980501915
1104	11	0.9899020499247868
1104	12	1.0730646561320185
1104	13	0.9741963558528397
1104	14	1.1430542973476747
1104	15	1.1588245674045439
1104	16	1.1386098164267935
1104	17	1.0601322276965206
1104	18	1.0928050529962334
1104	19	0.9909660397931219
1104	20	1.0912475955986238
1104	21	1.0974339647425637
1104	22	1.0597235007098647
1104	23	1.1301618059937653
1104	24	1.077476612826267
1104	25	1.0398441504451839
1105	1	0.5964621830368237
1105	2	0.5445018567007871
1105	3	0.5569957584436978
1105	4	0.4862875743128754
1105	5	0.468327101405535
1105	6	0.5610118647343825
1105	7	0.5481369840199974
1105	8	0.5567338020223644
1105	9	0.6422590669897446
1105	10	0.6566682062458185
1105	11	0.6506341633761181
1105	12	0.6871541781209913
1105	13	0.7020411260597754
1105	14	0.855136472407267
1105	15	0.8395805411029968
1105	16	0.834119069181888
1105	17	0.7309189018866888
1105	18	0.7883932103909501
1105	19	0.8738809052337686
1105	20	0.8408138964542289
1105	21	0.7694890551973117
1105	22	0.7860326014595245
1105	23	0.870451767877249
1105	24	0.8903386678936869
1105	25	0.8237500913242783
1106	1	0.7199380103693223
1106	2	0.7435738994648453
1106	3	0.6937964997945159
1106	4	0.6529477212085979
1106	5	0.6600036975071362
1106	6	0.7212440461122895
1106	7	0.8065608804959012
1106	8	0.8137404412637039
1106	9	0.8774765436410377
1106	10	0.9207802504477556
1106	11	1.003461435613204
1106	12	1.0401357222353482
1106	13	0.9737953990647839
1106	14	1.043323405165168
1106	15	1.0330224973444118
1106	16	1.1027585656723176
1106	17	1.07933880896762
1106	18	1.036168118294782
1106	19	1.0991729116774138
1106	20	1.0983170074422333
1106	21	1.1733057819692192
1106	22	1.1319661850815805
1106	23	1.1995039024326317
1106	24	1.1717161228077089
1106	25	1.1505270020312608
1107	1	0.4785870360857274
1107	2	0.6011781773885545
1107	3	0.5836436994989285
1107	4	0.49714462118780745
1107	5	0.3882013552961254
1107	6	0.4687720786806153
1107	7	0.583574342724198
1107	8	0.5258405300337898
1107	9	0.6739199861972338
1107	10	0.7006301152736043
1107	11	0.7168028921828338
1107	12	0.736753878307816
1107	13	0.7394688032391983
1107	14	0.8252971227605528
1107	15	0.8400557595405402
1107	16	0.888582239622977
1107	17	0.7853528641181313
1107	18	0.7373800755370823
1107	19	0.9083611533770082
1107	20	0.9344307152520486
1107	21	0.9100138253191687
1107	22	0.9590284069179447
1107	23	0.9348487630891853
1107	24	0.9188351332669669
1107	25	0.9082815968419453
1108	1	0.39416257612614913
1108	2	0.47279226174772404
1108	3	0.42289079259848705
1108	4	0.3094923862210095
1108	5	0.28537073638224086
1108	6	0.329825518403446
1108	7	0.45807039248928305
1108	8	0.5210812187657723
1108	9	0.5854583467841294
1108	10	0.499207669802594
1108	11	0.5669229740747426
1108	12	0.5828052838565227
1108	13	0.617974472679343
1108	14	0.6875438352726988
1108	15	0.6562648463932135
1108	16	0.6781152654241751
1108	17	0.7012008850635638
1108	18	0.6820945037372965
1108	19	0.7379032673432562
1108	20	0.6031091910650588
1108	21	0.6740553684042894
1108	22	0.6876010072486594
1108	23	0.7058004284872226
1108	24	0.6682680252063875
1108	25	0.7163613692769175
1109	1	0.538543207189754
1109	2	0.5432822844321805
1109	3	0.47432587276304616
1109	4	0.46785695874077504
1109	5	0.49512561289934354
1109	6	0.45152281549291473
1109	7	0.5366166008530584
1109	8	0.4966502525617642
1109	9	0.535548059319531
1109	10	0.5334463788392085
1109	11	0.5988968420947884
1109	12	0.6088281669610893
1109	13	0.6254342301991755
1109	14	0.7137996505674025
1109	15	0.7187752594553558
1109	16	0.7138968698687904
1109	17	0.737169283822972
1109	18	0.7598204832059423
1109	19	0.8019141225600421
1109	20	0.8147361759549057
1109	21	0.7680168336663087
1109	22	0.8013143692900044
1109	23	0.7606360943464807
1109	24	0.7878805471539181
1109	25	0.7482377550151895
1110	1	-0.015910277232119796
1110	2	0.05464904840815876
1110	3	-0.0024239962436531926
1110	4	0.015429350534546415
1110	5	-0.13169467580414107
1110	6	-0.028387821163335047
1110	7	0.0680961054392597
1110	8	0.07952519570203975
1110	9	0.16768212736695887
1110	10	0.07900371935846096
1110	11	0.08434466645436345
1110	12	0.09436247569070844
1110	13	0.12062173775647977
1110	14	0.21272187797177367
1110	15	0.10706428538339452
1110	16	0.06325376472291566
1110	17	-0.0016168257047084467
1110	18	-0.010033437668347744
1110	19	0.0016285283686556795
1110	20	-0.009464246678433597
1110	21	0.04525900717629838
1110	22	0.015302035766659117
1110	23	0.08302771538141229
1110	24	0.05121313195408561
1110	25	0.020150717882188474
1111	1	-0.10851623770508212
1111	2	-0.14643855494650282
1111	3	-0.084456123510801
1111	4	-0.13958238486273444
1111	5	-0.16021121070169642
1111	6	-0.1740018873768463
1111	7	-0.16256477299680938
1111	8	-0.16088622699773225
1111	9	-0.16817222803631893
1111	10	-0.24640883067868913
1111	11	-0.2123468385964813
1111	12	-0.22874017854198314
1111	13	-0.16561790829654655
1111	14	-0.21551444890431526
1111	15	-0.3113845353477842
1111	16	-0.2562056677643625
1111	17	-0.2947528195542475
1111	18	-0.28600592324396956
1111	19	-0.22818936044261307
1111	20	-0.2187944234671093
1111	21	-0.31161808348010567
1111	22	-0.26688507266841555
1111	23	-0.30926022506560713
1111	24	-0.28258732563399747
1111	25	-0.26666967324844393
1112	1	-0.2185007193125159
1112	2	-0.27877309592245325
1112	3	-0.20881176362071585
1112	4	-0.09908882705650512
1112	5	-0.2784952869257111
1112	6	-0.25460824896663325
1112	7	-0.22340582865679437
1112	8	-0.2145027561034354
1112	9	-0.24369634488370195
1112	10	-0.19868213539734114
1112	11	-0.2688001436984848
1112	12	-0.26859260104981786
1112	13	-0.23371339454551787
1112	14	-0.41403800384408385
1112	15	-0.39912192834718496
1112	16	-0.4070652178087997
1112	17	-0.3527649535306665
1112	18	-0.3663847979962185
1112	19	-0.38633630757213666
1112	20	-0.4508602172434095
1112	21	-0.422209761107986
1112	22	-0.34916180878497727
1112	23	-0.3546096937033312
1112	24	-0.40449425764865765
1112	25	-0.4020287731470873
1113	1	-0.4065703226519304
1113	2	-0.44584808517522845
1113	3	-0.44657388701467937
1113	4	-0.41602654067393274
1113	5	-0.5160111778847565
1113	6	-0.5156313356088305
1113	7	-0.6017938014798361
1113	8	-0.6097963965296103
1113	9	-0.7168271253664713
1113	10	-0.6656950436133258
1113	11	-0.6306249141549998
1113	12	-0.6086432787452836
1113	13	-0.603371100927589
1113	14	-0.7035601785032455
1113	15	-0.6670580888682736
1113	16	-0.7996776762083826
1113	17	-0.6443632929778715
1113	18	-0.7209240925831111
1113	19	-0.7634441244236356
1113	20	-0.7067815615630053
1113	21	-0.814669973740358
1113	22	-0.7542879707951826
1113	23	-0.7597790236005011
1113	24	-0.8217241687266679
1113	25	-0.7340650343125219
1114	1	-0.8364314720337802
1114	2	-1.4210915789307563
1114	3	-1.0096118577119775
1114	4	-0.9598499581679079
1114	5	-0.9805835646930277
1114	6	-0.9874189159445805
1114	7	-0.9986848702700684
1114	8	-1.0652091038148725
1114	9	-1.0751701554118398
1114	10	-1.1039479034216981
1114	11	-1.1024689689814409
1114	12	-1.134122249083056
1114	13	-1.1517358862484173
1114	14	-1.314027798833017
1114	15	-1.3077060759148296
1114	16	-1.3061114118437303
1114	17	-1.271625671047289
1114	18	-1.316868846255133
1114	19	-1.4003528283708135
1114	20	-1.3594322673629264
1114	21	-1.3821234518289387
1114	22	-1.348886574153731
1114	23	-1.4404206074055494
1114	24	-1.5093230318888686
1114	25	-1.6188803768990638
1115	1	-0.9527895908737811
1115	2	-1.316660025708007
1115	3	-1.1946132426709717
1115	4	-1.0987386600581104
1115	5	-1.2702001940183933
1115	6	-1.260456709547121
1115	7	-1.3731089902951723
1115	8	-1.4853734055325702
1115	9	-1.8110869807352046
1115	10	-1.4796449585430693
1115	11	-1.5423539297292521
1115	12	-1.4885729112337032
1115	13	-1.9089850421246766
1115	14	-1.719430856962724
1115	15	-1.824438133683401
1115	16	-1.9101783877594727
1115	17	-1.759445149531338
1115	18	-1.704958304087512
1115	19	-1.727880659034156
1115	20	-1.8240733175475299
1115	21	-1.8059196069022718
1115	22	-1.9216286176519617
1115	23	-1.8673912287437346
1115	24	-1.8412710205427274
1115	25	-1.7969542670910954
1116	1	-1.4170543290791606
1116	2	-1.4090806464268368
1116	3	-1.4986346609288042
1116	4	-1.544835657588493
1116	5	-1.662324918481211
1116	6	-1.6920834635856963
1116	7	-1.8986667505166963
1116	8	-2.0750193581547975
1116	9	-2.176133459120514
1116	10	-2.1082025200732986
1116	11	-2.2283441284295833
1116	12	-2.4380187162997586
1116	13	-2.259692808240608
1116	14	-2.5911144988203176
1116	15	-2.5966295237475663
1116	16	-2.7122674653766126
1116	17	-2.83310497074536
1116	18	-2.6812909000419936
1116	19	-2.6643252999923632
1116	20	-2.5766690428807877
1116	21	-2.4552532222425683
1116	22	-2.663217327886194
1116	23	-2.9032849310137294
1116	24	-2.9288218956135452
1116	25	-3.0915492308246613
1201	1	0.871152986883736
1201	2	1.0259023359633161
1201	3	0.979035623225279
1201	4	0.9171444914454909
1201	5	1.0332053495059128
1201	6	1.006357592765994
1201	7	1.0755643303245392
1201	8	1.0821713062079112
1201	9	1.2041481843915918
1201	10	1.2266660613082152
1201	11	1.2066595454613278
1201	12	1.282486560952556
1201	13	1.2754796163342732
1201	14	1.4556596042491066
1201	15	1.4777506278613615
1201	16	1.5166518037444092
1201	17	1.4737829079895732
1201	18	1.5123740981233667
1201	19	1.465504487124285
1201	20	1.5336262202369326
1201	21	1.5507063897871944
1201	22	1.5790285551948742
1201	23	1.6224649519643322
1201	24	1.5656645091508352
1201	25	1.6897176959724902
1202	1	0.7695369872255711
1202	2	0.9001846027985962
1202	3	0.8083202758067145
1202	4	0.7741185514157252
1202	5	0.9314098847635748
1202	6	0.9090692043807636
1202	7	0.9073431813207051
1202	8	0.9135314053410042
1202	9	1.0013345299880356
1202	10	1.0360895042909135
1202	11	1.0461155139470932
1202	12	1.0361277996685914
1202	13	1.0775357327464832
1202	14	1.1528493071855621
1202	15	1.2231005593410984
1202	16	1.2562448127451304
1202	17	1.287009911990907
1202	18	1.261897915398606
1202	19	1.338668657998646
1202	20	1.35219729563363
1202	21	1.351267167306446
1202	22	1.2471572247420681
1202	23	1.3308906545385994
1202	24	1.3187804767616598
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2211	5	-0.3313685563692985
2211	6	-0.34573710784666645
2211	7	-0.38493612604392524
2211	8	-0.3977868986669648
2211	9	-0.350200880162447
2211	10	-0.4376895052931431
2211	11	-0.49418300785677616
2211	12	-0.5246388252979273
2211	13	-0.4539594311289221
2211	14	-0.5826427050715708
2211	15	-0.5891467754327238
2211	16	-0.5148931894603876
2211	17	-0.5611887429091169
2211	18	-0.5609591068608992
2211	19	-0.6437230819063657
2211	20	-0.6563075953972088
2211	21	-0.6785406591321816
2211	22	-0.6961647778875957
2211	23	-0.6513329350015553
2211	24	-0.634973969142564
2211	25	-0.5888722786231497
2212	1	-0.4818387095655048
2212	2	-0.4948232372346908
2212	3	-0.5617713934429247
2212	4	-0.44872644337305445
2212	5	-0.4807371977367225
2212	6	-0.48602291622126614
2212	7	-0.5666078799311052
2212	8	-0.4691758586316439
2212	9	-0.6164299747484634
2212	10	-0.596914766430757
2212	11	-0.5932087026865318
2212	12	-0.753231099389275
2212	13	-0.8312170953770064
2212	14	-0.8657205114618947
2212	15	-0.8261010699819877
2212	16	-0.7869609163762803
2212	17	-0.7461702258715164
2212	18	-0.8326211817008726
2212	19	-0.9199525523918055
2212	20	-0.8897274194617708
2212	21	-0.987931497432875
2212	22	-0.9165846502820827
2212	23	-0.9117363142647648
2212	24	-1.0015947683395652
2212	25	-0.9118981789639378
2213	1	-0.729152562467906
2213	2	-0.7319964366121496
2213	3	-0.5981847719023499
2213	4	-0.6463590144017601
2213	5	-0.6461427024387731
2213	6	-0.6419138048684871
2213	7	-0.5894570296184725
2213	8	-0.7267300596274993
2213	9	-0.8438109679843535
2213	10	-0.7998170712703114
2213	11	-0.856436287654212
2213	12	-0.7847357651882163
2213	13	-0.818663711496896
2213	14	-1.0084808767416433
2213	15	-0.9936651071742588
2213	16	-1.0577010584533966
2213	17	-1.0505935194805787
2213	18	-1.0126986604649986
2213	19	-1.0916870680843331
2213	20	-1.0747310923629954
2213	21	-1.048045925160615
2213	22	-1.0032577038969848
2213	23	-1.055658051413424
2213	24	-1.0108891464471341
2213	25	-0.9760088077662061
2214	1	-1.0583000113268213
2214	2	-1.6437192537795475
2214	3	-1.1853224923507888
2214	4	-1.1424147875057216
2214	5	-1.1489347989119807
2214	6	-1.2102042353606066
2214	7	-1.1281457736212062
2214	8	-1.1806792376004367
2214	9	-1.5248910396014708
2214	10	-1.4218651904489334
2214	11	-1.2792748352701864
2214	12	-1.4177836370452752
2214	13	-1.4618444062207132
2214	14	-1.8226858826287966
2214	15	-1.779699218110757
2214	16	-1.8185636872068613
2214	17	-1.6152029288280048
2214	18	-1.5662781568388127
2214	19	-1.6855251625454457
2214	20	-1.7410395519219861
2214	21	-1.7676134636920935
2214	22	-1.7264103867106257
2214	23	-1.8197043065857486
2214	24	-1.7396126573370552
2214	25	-1.7265121255594806
2215	1	-1.2807477843765618
2215	2	-1.475341413532437
2215	3	-1.467133394872647
2215	4	-1.199402586674438
2215	5	-1.3466398319625554
2215	6	-1.3553380812756757
2215	7	-1.3424200419128383
2215	8	-1.3966333690748556
2215	9	-1.647078055967654
2215	10	-1.6175246818516342
2215	11	-1.6720206776256745
2215	12	-1.7952275697788949
2215	13	-1.9022035300918887
2215	14	-2.0672684383495934
2215	15	-2.2931237818490047
2215	16	-2.018860844890142
2215	17	-1.9638009684989228
2215	18	-2.0565537934161213
2215	19	-2.0623720322653654
2215	20	-1.9893852482474799
2215	21	-1.928149611297684
2215	22	-1.9679502604665373
2215	23	-2.029935344661034
2215	24	-2.099056554855359
2215	25	-1.9608110971158652
2216	1	-1.7363440469225964
2216	2	-1.7708299566052865
2216	3	-1.915525401391882
2216	4	-1.8007692375978621
2216	5	-1.8032892598006285
2216	6	-1.895377057152217
2216	7	-1.9356546204018912
2216	8	-1.960330548697577
2216	9	-2.2052616837528802
2216	10	-2.186306880479677
2216	11	-2.295360303564266
2216	12	-2.4216296977610057
2216	13	-2.2897762187562307
2216	14	-2.563466280111662
2216	15	-2.646999131320129
2216	16	-2.685309927273728
2216	17	-2.6796386064550504
2216	18	-2.774144321123579
2216	19	-2.7188321187557065
2216	20	-2.95023853741003
2216	21	-2.7465055063872796
2216	22	-2.804905622496797
2216	23	-2.7833514830893833
2216	24	-2.943510948120604
2216	25	-2.9825052539727324
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	44386.0
3	8.0
4	870.0
5	7018.0
6	8134.0
7	6318.0
8	5096.0
9	4423.0
10	4165.0
11	3749.0
12	3538.0
13	3474.0
14	3391.0
15	3473.0
16	3687.0
17	3828.0
18	4254.0
19	5048.0
20	6390.0
21	8258.0
22	10826.0
23	13517.0
24	16552.0
25	19201.0
26	21434.0
27	24406.0
28	28630.0
29	34948.0
30	45476.0
31	59195.0
32	77031.0
33	101734.0
34	138354.0
35	191593.0
36	279129.0
37	487174.0
38	1469775.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.37584277904997	19.36159287632437	13.02312898266921	26.23943536195646
2	15.565863639799794	21.543938420843	38.07272281438651	24.8174751249707
3	17.434396183812968	26.2037622562993	29.562236797848364	26.79960476203937
4	12.283619479654458	18.012083227589475	37.76712169082603	31.93717560193004
5	13.745552733510122	37.49627803877457	34.29478696384671	14.4633822638686
6	30.539379350576564	38.4963670262995	18.205729130259616	12.758524492864323
7	27.688744521508845	31.903073545101098	22.497521795652396	17.91066013773766
8	26.115628824276925	33.939172379227884	20.76747729294632	19.177721503548867
9	26.924096952272482	15.185278469558455	19.841593252864797	38.04903132530427
10	14.9955294823887	27.979723066123956	32.70116138171551	24.32358606977184
11	34.92286480290354	22.727274170992366	23.57636322576977	18.773497800334322
12	23.677396732066832	25.288113499757976	29.82684604175057	21.207643726424624
13	28.395826385249418	21.060110851715976	25.74062604221259	24.803436720822017
14	22.77527755824975	21.366839380871667	26.726083233169255	29.13179982770932
15	24.00418248438556	28.19168534300946	23.319287961254847	24.484844211350136
16	24.316914464195477	27.478766677539667	24.587649052508347	23.61666980575651
17	22.994531297893232	27.166556712413353	25.998617022188945	23.84029496750447
18	23.365657000692718	26.591036209050312	27.1836420708872	22.859664719369775
19	24.855159025718347	26.233169115117803	26.438031630137086	22.473640229026763
20	24.85127224563822	25.76838173404015	25.952508758500432	23.427837261821196
21	26.090341293849644	25.538055858710468	25.2309765173874	23.140626330052484
22	24.658933940872885	25.811632883236985	25.792321713917094	23.737111461973036
23	23.54504984080924	25.422052430041187	26.24319341994598	24.78970430920359
24	23.635687968491933	26.491932410113073	26.129113200355736	23.74326642103926
25	23.79479162067584	25.888765046741497	26.167997502122592	24.148445830460062
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	242.0
1	242.0
2	515.0
3	788.0
4	788.0
5	788.0
6	2075.5
7	3363.0
8	3363.0
9	3363.0
10	5239.0
11	7115.0
12	7115.0
13	7115.0
14	12013.5
15	16912.0
16	16912.0
17	16912.0
18	27878.0
19	38844.0
20	38844.0
21	38844.0
22	58693.0
23	78542.0
24	78542.0
25	78542.0
26	107860.0
27	137178.0
28	137178.0
29	137178.0
30	161838.5
31	186499.0
32	186499.0
33	186499.0
34	222787.5
35	259076.0
36	259076.0
37	259076.0
38	281585.0
39	304094.0
40	304094.0
41	304094.0
42	331011.5
43	357929.0
44	357929.0
45	357929.0
46	379574.5
47	401220.0
48	401220.0
49	401220.0
50	394427.5
51	387635.0
52	387635.0
53	387635.0
54	347709.0
55	307783.0
56	307783.0
57	307783.0
58	272125.5
59	236468.0
60	236468.0
61	236468.0
62	206525.0
63	176582.0
64	176582.0
65	176582.0
66	145522.5
67	114463.0
68	114463.0
69	114463.0
70	86522.5
71	58582.0
72	58582.0
73	58582.0
74	46420.5
75	34259.0
76	34259.0
77	34259.0
78	28680.0
79	23101.0
80	23101.0
81	23101.0
82	16782.5
83	10464.0
84	10464.0
85	10464.0
86	7815.0
87	5166.0
88	5166.0
89	5166.0
90	3587.0
91	2008.0
92	2008.0
93	2008.0
94	1059.0
95	110.0
96	110.0
97	110.0
98	85.0
99	60.0
100	60.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.41362776930985495
2	0.004605392501722257
3	0.0
4	0.00482772179490885
5	0.1581396501108629
6	0.03808183179010336
7	0.0010798851383348744
8	3.8113593117701446E-4
9	1.9056796558850723E-4
10	0.002477383552650594
11	0.001588066379904227
12	0.002255054259464002
13	0.004224256570545243
14	0.010004818193396629
15	0.003652552673779722
16	3.8113593117701446E-4
17	0.006479310830009246
18	5.081812415693526E-4
19	9.528398279425361E-4
20	0.003589030018583553
21	0.003525507363387384
22	0.0014610210695118887
23	0.0018739183282869877
24	0.0026361901906410165
25	0.006669878795597753
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	3148483.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	67.3606510754584
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.90325901610694	61.906633632814554
2	5.603696523443424	7.549372924968635
3	1.0151033510239236	2.0513406790155324
4	0.42016547825549316	1.132104806988855
5	0.2261868776865684	0.761804767284616
6	0.1454819713767588	0.5879856187007805
7	0.09693826596165202	0.4570877296511979
8	0.0759695649413096	0.40938874850927465
9	0.05685636688406555	0.3446893701986209
>10	0.3343274908481807	4.425058040447852
>50	0.05128929403967786	2.4485894400136035
>100	0.057143643371063015	8.402187847950428
>500	0.008802372917950987	4.097744780524196
>1k	0.004779783143028141	5.426011612931824
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTTCAAAGCAGGCCCGAGCCGCCTG	3607	0.11456310864629093	No Hit
GAATAGGACCGCGGTTCTATTTTGT	3463	0.10998947747216675	No Hit
CTATTGGAGCTGGAATTACCGCGGC	3389	0.1076391392299085	No Hit
GTCCTATTCCATTATTCCTAGCTGC	3182	0.101064544417105	No Hit
ATACAGGACTCTTTCGAGGCCCTGT	3155	0.10020698857195671	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	3.176132759808454E-5	0.0
3	0.0	0.0	0.0	3.176132759808454E-5	0.0
4	0.0	0.0	0.0	6.352265519616908E-5	0.0
5	0.0	0.0	0.0	6.352265519616908E-5	0.0
6	0.0	0.0	0.0	6.352265519616908E-5	0.0
7	0.0	0.0	0.0	6.352265519616908E-5	0.0
8	0.0	0.0	0.0	6.352265519616908E-5	0.0
9	0.0	0.0	0.0	9.528398279425361E-5	0.0
10	0.0	0.0	0.0	1.2704531039233816E-4	0.0
11	0.0	0.0	0.0	1.2704531039233816E-4	0.0
12	0.0	0.0	0.0	1.588066379904227E-4	0.0
13	0.0	0.0	0.0	1.588066379904227E-4	3.176132759808454E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1305	0.0	15.352117	1
GTATCAA	4310	0.0	13.369622	1
CTCGACG	100	1.0179065E-8	13.291217	13
CCGACCA	420	0.0	11.980179	9
CGTCTTA	265	0.0	11.822376	15
CGCATCG	340	0.0	11.727544	13
CGTCGTA	365	0.0	11.704594	10
TCGACGC	115	7.147901E-8	11.55758	14
ATACCGT	470	0.0	11.517509	6
ACCGTCG	380	0.0	11.492405	8
ATCGCCA	380	0.0	11.492405	16
CCGTCGT	390	0.0	11.441157	9
CGAACGA	150	1.7826096E-10	11.392472	16
CGACCAT	430	0.0	11.260001	10
TATACCG	85	5.263676E-5	11.189527	5
TACACCG	85	5.263676E-5	11.189527	5
CATCGCC	400	0.0	11.1551285	15
GCATCGC	375	0.0	11.139305	14
TCTCGTC	290	0.0	11.130575	12
ACCGCTA	60	0.0059069	11.076014	8
>>END_MODULE