FastQCFastQC Report
Thu 2 Feb 2017
SRR4050219_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4050219_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3465111
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNN77010.2222439627475137No Hit
GNNNNNNNNNNNNNNNNNNNNNNNN58420.16859488772509743No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG40850.11788944134834352No Hit
GTCCTATTCCATTATTCCTAGCTGC40150.11586930404249675No Hit
GTATCTGATCGTCTTCGAACCTCCG40050.11558071299880437No Hit
CTATTGGAGCTGGAATTACCGCGGC38970.1124639297269265No Hit
GAATAGGACCGCGGTTCTATTTTGT38310.11055922883855668No Hit
CTCTTAATCATGGCCTCAGTTCCGA35680.10296928438944669No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA15150.015.4905691
GTATCAA52500.013.6275791
CGAACGA2300.013.2172816
TATCACG550.00306693812.0923882
CCGTCCA951.0418171E-611.9992029
CGCATCG4700.011.92473813
ATCGCCA5050.011.85138616
ACGCTTA1052.7247006E-711.76146417
CTCGACG1157.090057E-811.56444813
TCGCCAG5550.011.29687417
CGCCAGT5300.011.29202918
ACGAACG2700.011.25916415
CATCGCC5400.011.0832415
TCGACGC1408.367351E-910.85689414
GATAACG2800.010.85642111
CGAGCCG6900.010.7390415
GGAGCGT3100.010.7263396
AGGCCCG7100.010.70335910
CGCAGAT1605.456968E-1010.68678958
CGCTTAC1252.2439781E-710.63960218
Produced by FastQC (version 0.11.5)