##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4050219_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3465111
Sequences flagged as poor quality	0
Sequence length	25
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.89346113299112	31.0	30.0	34.0	26.0	34.0
2	30.013677483924756	31.0	31.0	34.0	26.0	34.0
3	30.133574941755114	31.0	31.0	34.0	26.0	34.0
4	33.53956741934097	37.0	35.0	37.0	28.0	37.0
5	33.60663338057569	37.0	35.0	37.0	28.0	37.0
6	33.691940027318026	37.0	35.0	37.0	29.0	37.0
7	33.62727860665935	37.0	35.0	37.0	29.0	37.0
8	33.54942972966811	37.0	35.0	37.0	28.0	37.0
9	35.056145964732444	39.0	35.0	39.0	28.0	39.0
10	34.94540030607966	39.0	35.0	39.0	28.0	39.0
11	34.98940322546666	39.0	35.0	39.0	28.0	39.0
12	34.87773465265615	39.0	35.0	39.0	27.0	39.0
13	34.87993429359117	39.0	35.0	39.0	27.0	39.0
14	35.72027851344445	40.0	36.0	41.0	27.0	41.0
15	35.84372304379283	40.0	36.0	41.0	27.0	41.0
16	35.86500230439948	40.0	36.0	41.0	27.0	41.0
17	35.851362337310405	40.0	36.0	41.0	27.0	41.0
18	35.67764149546724	40.0	36.0	41.0	27.0	41.0
19	35.82360103327137	40.0	36.0	41.0	27.0	41.0
20	35.78406896633326	40.0	36.0	41.0	27.0	41.0
21	35.74895926854868	40.0	36.0	41.0	27.0	41.0
22	35.66454840840596	40.0	36.0	41.0	27.0	41.0
23	35.54835588239453	40.0	36.0	41.0	27.0	41.0
24	35.48186277438154	40.0	36.0	41.0	26.0	41.0
25	35.204960245140775	40.0	36.0	41.0	25.0	41.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	1.0098262830083122
1101	2	1.0031113031239443
1101	3	0.9355655474066928
1101	4	1.0362930294187578
1101	5	1.0125834982938144
1101	6	0.9760819235136466
1101	7	1.0192965067933457
1101	8	1.0030982700991302
1101	9	1.1110413510951176
1101	10	1.0030425519915909
1101	11	1.1465250446615798
1101	12	1.2110659717344916
1101	13	1.2541482285502354
1101	14	1.6789616175050597
1101	15	1.5258334079785811
1101	16	1.4385400285327634
1101	17	1.474603683497108
1101	18	1.603435699882418
1101	19	1.524720293372468
1101	20	1.6028891133479277
1101	21	1.5838395533771887
1101	22	1.541149748519608
1101	23	1.5053320573532645
1101	24	1.5770105365662488
1101	25	1.6401301466113338
1102	1	1.1414805888806612
1102	2	1.1469148759932892
1102	3	1.131269728475786
1102	4	1.2515366999279252
1102	5	1.134700504706231
1102	6	1.1401831543849426
1102	7	1.1487441835574046
1102	8	1.0832598675807787
1102	9	1.2864811721058587
1102	10	1.322214227223938
1102	11	1.3469466478551055
1102	12	1.4784049153444272
1102	13	1.4341530695589597
1102	14	1.8319490193561663
1102	15	1.6909918485590367
1102	16	1.6644793827319546
1102	17	1.6071685600577936
1102	18	1.7479186788701355
1102	19	1.5484002540296657
1102	20	1.657886430302085
1102	21	1.6289601879847666
1102	22	1.7113284944422062
1102	23	1.7424415909387179
1102	24	1.8222479701144394
1102	25	1.7702194491346788
1103	1	0.7845024697162124
1103	2	0.7947432143500528
1103	3	0.808758858795894
1103	4	0.8730148311126271
1103	5	0.9533940365483957
1103	6	0.9380448121868881
1103	7	0.8765674682199602
1103	8	0.8562540464929711
1103	9	1.0557051429431752
1103	10	1.055383497096571
1103	11	1.01084156560632
1103	12	1.0576052279055546
1103	13	1.1349802531068107
1103	14	1.4769180944488411
1103	15	1.3011687814716808
1103	16	1.241698948103874
1103	17	1.2669678581788801
1103	18	1.3611433076216457
1103	19	1.3763102920465187
1103	20	1.3144643875131052
1103	21	1.3005625947331225
1103	22	1.3772081373075622
1103	23	1.2466888040996338
1103	24	1.3426263607117335
1103	25	1.2817925299285804
1104	1	0.8663913629921645
1104	2	0.9105701974116052
1104	3	0.8141857911868868
1104	4	0.9147046121105049
1104	5	0.8633569713610498
1104	6	0.8467492909381562
1104	7	0.8919475467922382
1104	8	0.8789718970082347
1104	9	1.0093621427194321
1104	10	1.1197805996616879
1104	11	1.1253126175798371
1104	12	1.1869143644002023
1104	13	1.192761145698384
1104	14	1.4926065560379271
1104	15	1.3235842537608065
1104	16	1.344833448834322
1104	17	1.2601350644541327
1104	18	1.4423245404298441
1104	19	1.3522773923742335
1104	20	1.2885928702916942
1104	21	1.2721570558484032
1104	22	1.3445687274551048
1104	23	1.2637100001881265
1104	24	1.3431440065705615
1104	25	1.3370968165265467
1105	1	0.6109211159158896
1105	2	0.7281657078937265
1105	3	0.6530603956802246
1105	4	0.7161237807348115
1105	5	0.7529274129279244
1105	6	0.690563630402238
1105	7	0.7447818653111753
1105	8	0.7616401106868764
1105	9	0.8272488320501452
1105	10	0.8730767139877784
1105	11	0.8346613764687945
1105	12	0.7973975191587002
1105	13	0.7704507501598883
1105	14	1.1729071411012981
1105	15	1.0009905576754434
1105	16	0.9937670132645025
1105	17	0.9293881218718312
1105	18	1.0283194266958162
1105	19	0.9502929754958913
1105	20	0.9460213528193719
1105	21	0.9342433654290545
1105	22	0.9147768101151996
1105	23	0.8720270198663798
1105	24	0.8674544960120514
1105	25	0.9016111948250867
1106	1	0.5600082517703413
1106	2	0.5230001388738046
1106	3	0.5008474240178735
1106	4	0.694474041678319
1106	5	0.5225182928207559
1106	6	0.4842850986352545
1106	7	0.5062215846549805
1106	8	0.5797036195141487
1106	9	0.7059666389207067
1106	10	0.6016019659588565
1106	11	0.6724573429503735
1106	12	0.6930125709016366
1106	13	0.7279336453870116
1106	14	1.0456922454139388
1106	15	0.9247550097702728
1106	16	0.8636138075340369
1106	17	0.8834583301161061
1106	18	1.086323637453134
1106	19	0.9513985469389397
1106	20	0.9027158930840713
1106	21	0.9804060594603143
1106	22	0.9833064282895805
1106	23	0.8534861242384437
1106	24	0.9517588714354304
1106	25	0.9374175289440316
1107	1	0.43896132263142107
1107	2	0.48396172104492763
1107	3	0.5038791853971638
1107	4	0.6001574569017691
1107	5	0.4053236340588029
1107	6	0.48247938686360925
1107	7	0.5000691923015665
1107	8	0.48248941821135816
1107	9	0.4915398368448436
1107	10	0.5894944337491097
1107	11	0.5295085167817817
1107	12	0.4690656500211574
1107	13	0.4392288174147225
1107	14	0.8947496138069084
1107	15	0.7240504478220942
1107	16	0.6152799662700374
1107	17	0.5525542208474334
1107	18	0.6280503239839135
1107	19	0.6214087128605215
1107	20	0.6028390754048729
1107	21	0.5691643845900174
1107	22	0.6459992135547665
1107	23	0.598395891635441
1107	24	0.6147948205020413
1107	25	0.643136426230221
1108	1	0.3294965785483903
1108	2	0.3060510536069643
1108	3	0.24481776284802592
1108	4	0.39779739173064144
1108	5	0.2455385360279152
1108	6	0.3623311420084008
1108	7	0.30345681721731665
1108	8	0.26140029616409777
1108	9	0.35470658046438786
1108	10	0.43521435003853526
1108	11	0.4158748726295869
1108	12	0.3848409546926348
1108	13	0.3898940971605498
1108	14	0.7197380001138995
1108	15	0.5680491316625265
1108	16	0.5862014018038693
1108	17	0.5244831199259465
1108	18	0.6877085470623427
1108	19	0.5803611276772216
1108	20	0.5208369914855453
1108	21	0.5199265011302856
1108	22	0.4705907352935128
1108	23	0.5437318572879164
1108	24	0.6313799878789723
1108	25	0.534053403804208
1109	1	0.180209643944913
1109	2	0.2221396592240552
1109	3	0.12799018185142685
1109	4	0.2910072248181521
1109	5	0.18765930848537948
1109	6	0.25365502366381776
1109	7	0.26774781259678804
1109	8	0.30187598216051725
1109	9	0.3211902231779078
1109	10	0.32977771788988974
1109	11	0.34483917729252056
1109	12	0.35771534879567213
1109	13	0.3548796998638366
1109	14	0.6429516278377463
1109	15	0.4908517710810685
1109	16	0.5024957762984243
1109	17	0.45701630582362895
1109	18	0.5447814916486351
1109	19	0.43269108590187955
1109	20	0.463046259670179
1109	21	0.48166427287174685
1109	22	0.5206467619830235
1109	23	0.5520857921197617
1109	24	0.5586742748049929
1109	25	0.5119530983445699
1110	1	-0.08525018860354905
1110	2	-0.12513999567840628
1110	3	-0.1415320653420764
1110	4	-0.029524531876198523
1110	5	-0.151453877776639
1110	6	-0.10973240275347251
1110	7	-0.17578257123904706
1110	8	-0.19206030375954697
1110	9	-0.1710144401030007
1110	10	-0.17458462408207964
1110	11	-0.275147188757181
1110	12	-0.18306429256421097
1110	13	-0.21723427056243594
1110	14	-0.04961111427663667
1110	15	-0.08241185006088614
1110	16	-0.2328759707497028
1110	17	-0.28442675872813084
1110	18	-0.13739993387256533
1110	19	-0.1878163594162885
1110	20	-0.3049358210507691
1110	21	-0.242981817124722
1110	22	-0.23720661202808202
1110	23	-0.204191649957977
1110	24	-0.22003316927312966
1110	25	-0.3110195531520148
1111	1	-0.4090097065265539
1111	2	-0.4464334251517883
1111	3	-0.5235050402776658
1111	4	-0.2983333816793845
1111	5	-0.40254672882870324
1111	6	-0.5120871762000192
1111	7	-0.549460724727517
1111	8	-0.5279842872249887
1111	9	-0.6752699945929663
1111	10	-0.4544830280768011
1111	11	-0.5466944550439337
1111	12	-0.47069086244126623
1111	13	-0.5942407518348247
1111	14	-0.39326975929759556
1111	15	-0.578270153691328
1111	16	-0.6088430217539127
1111	17	-0.5669716286629978
1111	18	-0.4576549748366716
1111	19	-0.6749791627404775
1111	20	-0.7115117096634549
1111	21	-0.6270957904514063
1111	22	-0.6617146950047896
1111	23	-0.6599380074020047
1111	24	-0.842362505418393
1111	25	-0.8031093245809231
1112	1	-0.7043119955544945
1112	2	-0.6735670735155459
1112	3	-0.7109061190653776
1112	4	-0.6129689443397339
1112	5	-0.5644207340434519
1112	6	-0.654539852338992
1112	7	-0.6291223900190346
1112	8	-0.608444523284561
1112	9	-0.7419635452533129
1112	10	-0.7530233932405537
1112	11	-0.7421000728228222
1112	12	-0.6465839571492324
1112	13	-0.7698014533768216
1112	14	-0.6527729054317604
1112	15	-0.7047856745485106
1112	16	-0.9018268596495318
1112	17	-0.9670849743806045
1112	18	-0.8497987749488232
1112	19	-0.8486746552257429
1112	20	-0.8965334728801366
1112	21	-0.9366353716518532
1112	22	-0.8536983820445982
1112	23	-0.9966893311742098
1112	24	-0.812221790062658
1112	25	-0.8937412843011572
1113	1	-0.9633684561355125
1113	2	-1.0989376541402187
1113	3	-0.9776846314200114
1113	4	-0.8627453814603854
1113	5	-0.9682804495474926
1113	6	-1.0415182511591325
1113	7	-1.093662796843752
1113	8	-1.1307353909248619
1113	9	-1.1833139613464425
1113	10	-1.2859466332489333
1113	11	-1.2803180782106978
1113	12	-1.2393017421301664
1113	13	-1.2519819845286193
1113	14	-1.1845062105692747
1113	15	-1.2944469917187291
1113	16	-1.3498220177852005
1113	17	-1.3208072423108987
1113	18	-1.2840172351298875
1113	19	-1.2821333193283522
1113	20	-1.5583203192974295
1113	21	-1.482170706928116
1113	22	-1.5123180558999323
1113	23	-1.8434333448783349
1113	24	-1.654959506134965
1113	25	-1.7188515844999657
1114	1	-2.450600066698563
1114	2	-2.7117949825541565
1114	3	-2.330954536223615
1114	4	-5.351706866039766
1114	5	-2.9931622259707247
1114	6	-2.5729746291867777
1114	7	-2.3133794813443522
1114	8	-2.5733929877997284
1114	9	-3.104320903416415
1114	10	-2.867759481426212
1114	11	-2.6228299007065274
1114	12	-2.751615199678554
1114	13	-2.6906099320569155
1114	14	-2.7366828918023245
1114	15	-3.437722436661595
1114	16	-3.2895318411448002
1114	17	-3.095301138528569
1114	18	-2.9793538945361604
1114	19	-3.0231537057201194
1114	20	-3.21735056808204
1114	21	-3.226992185327717
1114	22	-3.1982588037913757
1114	23	-3.364422227823958
1114	24	-3.403157565547218
1114	25	-3.4491261564101663
1115	1	-2.997137186257447
1115	2	-2.482055298534572
1115	3	-2.1076595598567565
1115	4	-2.0036119740547527
1115	5	-1.9715371549111609
1115	6	-2.0678487770280363
1115	7	-2.1073247060971916
1115	8	-2.022749898748195
1115	9	-2.330646698060278
1115	10	-2.1712115083252
1115	11	-2.2309869770380644
1115	12	-2.5735781317184774
1115	13	-2.7602695167132083
1115	14	-3.0313638165686
1115	15	-2.8278604590216716
1115	16	-2.6972993353644057
1115	17	-2.7076532312160424
1115	18	-2.6690897686955637
1115	19	-2.5042442998803764
1115	20	-2.5955519459925895
1115	21	-2.6243249607118884
1115	22	-2.691395995677553
1115	23	-2.707263132466693
1115	24	-3.0317223035898664
1115	25	-3.1243692435045034
1116	1	-1.5967480514620753
1116	2	-1.6366309039600573
1116	3	-1.7469569134888907
1116	4	-1.5465091386157468
1116	5	-1.7693231066948805
1116	6	-1.9500432167032429
1116	7	-2.0699668758717706
1116	8	-2.0472178875169895
1116	9	-2.2842869718888963
1116	10	-2.7265286369563952
1116	11	-2.450729391160401
1116	12	-2.5159517911559135
1116	13	-2.4082278153241745
1116	14	-3.299607428350889
1116	15	-3.2162129018710957
1116	16	-3.1872714719730126
1116	17	-2.752009389533292
1116	18	-2.675256199459511
1116	19	-2.6650374774062513
1116	20	-2.7620662791765653
1116	21	-2.895352006470034
1116	22	-2.6838591655400705
1116	23	-3.060257779638583
1116	24	-3.090944677846039
1116	25	-2.957697560415376
1201	1	1.106214421198903
1201	2	1.1258246296710652
1201	3	1.0626757641366815
1201	4	1.197259104675183
1201	5	1.083919352406383
1201	6	0.9878204580707646
1201	7	1.0843902655468085
1201	8	1.086644080614974
1201	9	1.2981906005092654
1201	10	1.3139197949181423
1201	11	1.2264258240746742
1201	12	1.2792291959987452
1201	13	1.2960172790530962
1201	14	1.6903838031917289
1201	15	1.5222634648430216
1201	16	1.497541867861493
1201	17	1.3862375519573575
1201	18	1.509842466418256
1201	19	1.4854629982499858
1201	20	1.4953954648105423
1201	21	1.4972125244513421
1201	22	1.499727961125636
1201	23	1.557477385869646
1201	24	1.5012229454078252
1201	25	1.5359452656714652
1202	1	0.9638936148935819
1202	2	1.0006669106514323
1202	3	0.9191914886667867
1202	4	1.0618497344581712
1202	5	1.0609876070406443
1202	6	0.9248677511116696
1202	7	0.8936146734337527
1202	8	1.0131437390974583
1202	9	1.1873076467051291
1202	10	1.2251912161069995
1202	11	1.1823542963808151
1202	12	1.2084124883800769
1202	13	1.2065214485706548
1202	14	1.5357832947428633
1202	15	1.4178833875406838
1202	16	1.3817872357536558
1202	17	1.3864912001235723
1202	18	1.4897981356158567
1202	19	1.3731655135095977
1202	20	1.3320853408137978
1202	21	1.4088358314235734
1202	22	1.358880492200825
1202	23	1.4756190877109034
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1202	25	1.3613497947931563
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2211	9	-0.09058906639717179
2211	10	-0.15021160501326847
2211	11	-0.18842958709511493
2211	12	-0.10607601201865435
2211	13	-0.2726253678284678
2211	14	-0.038523149197843054
2211	15	-0.15638232167999888
2211	16	-0.1990441365216853
2211	17	-0.41863192823284123
2211	18	-0.2507395831288619
2211	19	-0.33186529011488375
2211	20	-0.25077324079449426
2211	21	-0.3585057711065929
2211	22	-0.36809110474912643
2211	23	-0.3140116338017336
2211	24	-0.2871324190205371
2211	25	-0.28908874585848565
2212	1	-0.3663692030237655
2212	2	-0.41051697806764054
2212	3	-0.42932878163180277
2212	4	-0.32501457458558747
2212	5	-0.4214505108477553
2212	6	-0.35227556444819186
2212	7	-0.46688781792038014
2212	8	-0.40919248647801965
2212	9	-0.5468634683455065
2212	10	-0.5513335102832215
2212	11	-0.5682240738358075
2212	12	-0.5761485418527172
2212	13	-0.49843847343520054
2212	14	-0.5508506047460457
2212	15	-0.636992494674729
2212	16	-0.6094248110684788
2212	17	-0.7174503002584842
2212	18	-0.47369451802654794
2212	19	-0.6642143091960122
2212	20	-0.6615268040455007
2212	21	-0.6732559324118839
2212	22	-0.8996303523011875
2212	23	-0.8947260946101849
2212	24	-0.6726521459631343
2212	25	-0.7853738892321331
2213	1	-0.7075826278877564
2213	2	-0.49124537428236437
2213	3	-0.5492188279652837
2213	4	-0.4742087893419509
2213	5	-0.5394075532125058
2213	6	-0.623994671230399
2213	7	-0.5990115395107551
2213	8	-0.5139727069438038
2213	9	-0.5127730540378721
2213	10	-0.6909069953391551
2213	11	-0.6619066021465585
2213	12	-0.6451726985959141
2213	13	-0.7626940139030367
2213	14	-0.5446379972469941
2213	15	-0.7170592186256144
2213	16	-0.7760941816335034
2213	17	-0.8075203147047958
2213	18	-0.7813465033127684
2213	19	-0.7769374784246139
2213	20	-0.708515937342149
2213	21	-0.7492234119779795
2213	22	-0.8909531438504175
2213	23	-0.8769820281854592
2213	24	-0.8733660846302485
2213	25	-0.9569540194808823
2214	1	-1.2862243871630241
2214	2	-1.1457326473772298
2214	3	-1.1184852653878075
2214	4	-1.083630710149059
2214	5	-0.9855672692794073
2214	6	-1.1573429024818012
2214	7	-1.2591236639019456
2214	8	-1.1326646592774878
2214	9	-1.3184962921115684
2214	10	-1.3518733218229997
2214	11	-1.3361762408656972
2214	12	-1.38997172631624
2214	13	-1.546471895806853
2214	14	-1.3286346409567429
2214	15	-1.5648086224940911
2214	16	-1.419742883121927
2214	17	-1.5050411561935988
2214	18	-1.4513582998638341
2214	19	-1.6991387141822614
2214	20	-1.7083698253009132
2214	21	-1.6874982891009722
2214	22	-1.7802289907202962
2214	23	-1.8098957000922837
2214	24	-1.794592390244297
2214	25	-1.7662012334338542
2215	1	-1.7159650294067106
2215	2	-1.9049411619034657
2215	3	-1.7261344127484044
2215	4	-1.9231738280072719
2215	5	-1.8258560932094063
2215	6	-1.5523720054688042
2215	7	-1.6894612174272794
2215	8	-1.8503317134177841
2215	9	-2.221110129904126
2215	10	-1.8598602998483074
2215	11	-2.0304460825364146
2215	12	-2.148510971598668
2215	13	-2.144962536653665
2215	14	-2.802224711142294
2215	15	-2.5606642713209524
2215	16	-2.251109439506493
2215	17	-2.349420803167206
2215	18	-2.214759888453564
2215	19	-2.415371851424588
2215	20	-2.5801081015789222
2215	21	-2.4129237930308776
2215	22	-2.7743045219283147
2215	23	-2.419521700503971
2215	24	-2.572519937518358
2215	25	-2.465996709044134
2216	1	-1.7108132161099476
2216	2	-1.5861570791069397
2216	3	-1.6263045178399516
2216	4	-1.521623374419093
2216	5	-2.1108427023049465
2216	6	-1.743173452352714
2216	7	-1.8433276737178055
2216	8	-2.2738499016864324
2216	9	-2.256377217540795
2216	10	-2.3324840593036384
2216	11	-2.343229513912952
2216	12	-2.5056203542018736
2216	13	-2.4988636431749853
2216	14	-2.424270542195913
2216	15	-2.4093598419916376
2216	16	-2.458411385079394
2216	17	-2.6465727675620485
2216	18	-2.8762980016390927
2216	19	-2.738715710822376
2216	20	-2.7578363527622898
2216	21	-2.8362049924448556
2216	22	-3.0942394757269156
2216	23	-2.7023874933998755
2216	24	-2.8101001757177357
2216	25	-2.8309292276380162
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	131912.0
3	8.0
4	965.0
5	5748.0
6	7014.0
7	6436.0
8	5754.0
9	5291.0
10	5085.0
11	4851.0
12	4708.0
13	4789.0
14	4682.0
15	4865.0
16	5008.0
17	5295.0
18	6051.0
19	7003.0
20	9146.0
21	11605.0
22	14322.0
23	17179.0
24	20230.0
25	23466.0
26	26548.0
27	29837.0
28	34546.0
29	40851.0
30	51938.0
31	66593.0
32	85937.0
33	112988.0
34	153396.0
35	212151.0
36	311236.0
37	537145.0
38	1490532.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.23573768576138	18.72776055475177	12.667235132581947	25.369266626904906
2	15.734649027259055	20.73533743122496	39.274956677024676	24.25505686449131
3	17.656809676673532	25.882789653646004	29.457546046340866	27.002854623339594
4	11.951038918296819	17.175012512450724	38.718940001527926	32.15500856772453
5	14.08649243833953	37.04108180189917	34.055144703641886	14.81728105611942
6	30.61395099887227	37.885054211460286	18.5336855511559	12.967309238511538
7	27.94967313747635	31.76297723686325	22.413131333969265	17.874218291691136
8	26.33161504539197	33.312065379781146	20.929148078998246	19.427171495828635
9	27.30500688100781	15.21869170499375	19.93316256464472	37.54313884935372
10	15.352273242294403	27.474140365434025	32.45873672736316	24.71484966490841
11	34.92577052053317	22.75848704230868	23.559063701555015	18.75667873560313
12	23.93851526658783	25.062365079042255	29.65890238623058	21.340217268139334
13	28.376721985782222	21.07652201651991	25.737958628471752	24.808797369226117
14	22.85332473425657	21.30334114803485	26.86877402149188	28.974560096216695
15	24.189210154287576	27.83158348571505	23.444268384730567	24.534937975266807
16	24.419193856180893	27.362739150343664	24.57934410454162	23.638722888933827
17	23.097688391985496	27.04453535400493	25.92510808423597	23.932668169773606
18	23.372489158837343	26.40159396904218	27.299770422437824	22.92614644968265
19	24.986773774159108	26.08935147188065	26.447193149381544	22.4766816045787
20	25.06394961665096	25.61360833896028	25.79697318706759	23.525468857321172
21	26.477614932385023	25.37235812040681	25.05904416582048	23.090982781387687
22	25.01754140279506	25.544350041271326	25.679102879637362	23.759005676296255
23	23.692883427205018	25.266993147391375	26.07399422678256	24.96612919862105
24	23.718416786693798	26.398927470294918	26.022356572542343	23.860299170468938
25	23.915548296340972	25.78448615974959	26.002378806657173	24.297586737252264
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	12845.0
1	12845.0
2	11630.5
3	10416.0
4	10416.0
5	10416.0
6	7094.0
7	3772.0
8	3772.0
9	3772.0
10	5857.5
11	7943.0
12	7943.0
13	7943.0
14	13096.5
15	18250.0
16	18250.0
17	18250.0
18	29926.0
19	41602.0
20	41602.0
21	41602.0
22	62725.0
23	83848.0
24	83848.0
25	83848.0
26	115031.0
27	146214.0
28	146214.0
29	146214.0
30	173444.0
31	200674.0
32	200674.0
33	200674.0
34	240245.0
35	279816.0
36	279816.0
37	279816.0
38	305018.0
39	330220.0
40	330220.0
41	330220.0
42	358307.5
43	386395.0
44	386395.0
45	386395.0
46	411585.5
47	436776.0
48	436776.0
49	436776.0
50	430158.0
51	423540.0
52	423540.0
53	423540.0
54	380374.0
55	337208.0
56	337208.0
57	337208.0
58	298617.5
59	260027.0
60	260027.0
61	260027.0
62	228786.0
63	197545.0
64	197545.0
65	197545.0
66	163070.5
67	128596.0
68	128596.0
69	128596.0
70	98270.5
71	67945.0
72	67945.0
73	67945.0
74	54442.5
75	40940.0
76	40940.0
77	40940.0
78	34187.5
79	27435.0
80	27435.0
81	27435.0
82	20251.5
83	13068.0
84	13068.0
85	13068.0
86	10028.5
87	6989.0
88	6989.0
89	6989.0
90	4856.5
91	2724.0
92	2724.0
93	2724.0
94	1489.0
95	254.0
96	254.0
97	254.0
98	161.5
99	69.0
100	69.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.22663054661163812
2	0.6454338692180424
3	0.6428365498248108
4	0.6504842124826593
5	0.6659526924245717
6	0.6833835914635923
7	0.6767459974586673
8	0.6727923001600814
9	0.6645674554148482
10	0.6656929604852485
11	0.6700218261406344
12	0.6718688088202658
13	0.6661258470507871
14	0.6603251670725699
15	0.6665587336163257
16	0.6736580732911587
17	0.6738889461261125
18	0.6677130977910952
19	0.6662412834682641
20	0.6735426368736818
21	0.6694157849488804
22	0.6710896130022963
23	0.6740332416479587
24	0.6789970075994679
25	0.6651734966066022
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	3465111.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	65.53039123648824
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.78098245012343	60.1444368802585
2	5.648089508495859	7.402430304608765
3	1.0220447603787173	2.0092497902646067
4	0.4399271910802251	1.1531440378822588
5	0.2312338454374048	0.7576422179315394
6	0.15401547509635893	0.6055616603722808
7	0.09793825608195236	0.4492552566648799
8	0.07506739533485389	0.3935356628317686
9	0.055366138801302936	0.32653482632127845
>10	0.3692537225329963	4.667969324388886
>50	0.051723031193657294	2.4165146256900734
>100	0.0586609269112172	8.340455889711837
>500	0.010345946761660493	4.655606126372764
>1k	0.005262162574455911	6.281896110002609
>5k	8.918919617721883E-5	0.39576728669807654
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
NNNNNNNNNNNNNNNNNNNNNNNNN	7701	0.2222439627475137	No Hit
GNNNNNNNNNNNNNNNNNNNNNNNN	5842	0.16859488772509743	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG	4085	0.11788944134834352	No Hit
GTCCTATTCCATTATTCCTAGCTGC	4015	0.11586930404249675	No Hit
GTATCTGATCGTCTTCGAACCTCCG	4005	0.11558071299880437	No Hit
CTATTGGAGCTGGAATTACCGCGGC	3897	0.1124639297269265	No Hit
GAATAGGACCGCGGTTCTATTTTGT	3831	0.11055922883855668	No Hit
CTCTTAATCATGGCCTCAGTTCCGA	3568	0.10296928438944669	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	8.657731310771862E-5	0.0
5	0.0	0.0	0.0	1.1543641747695817E-4	0.0
6	0.0	0.0	0.0	1.1543641747695817E-4	0.0
7	0.0	0.0	0.0	1.1543641747695817E-4	0.0
8	0.0	0.0	0.0	1.1543641747695817E-4	0.0
9	0.0	0.0	0.0	1.7315462621543724E-4	0.0
10	0.0	0.0	0.0	2.3087283495391633E-4	0.0
11	0.0	0.0	0.0	2.3087283495391633E-4	0.0
12	0.0	0.0	0.0	2.3087283495391633E-4	5.771820873847908E-5
13	0.0	0.0	0.0	2.8859104369239545E-4	5.771820873847908E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1515	0.0	15.490569	1
GTATCAA	5250	0.0	13.627579	1
CGAACGA	230	0.0	13.21728	16
TATCACG	55	0.003066938	12.092388	2
CCGTCCA	95	1.0418171E-6	11.999202	9
CGCATCG	470	0.0	11.924738	13
ATCGCCA	505	0.0	11.851386	16
ACGCTTA	105	2.7247006E-7	11.761464	17
CTCGACG	115	7.090057E-8	11.564448	13
TCGCCAG	555	0.0	11.296874	17
CGCCAGT	530	0.0	11.292029	18
ACGAACG	270	0.0	11.259164	15
CATCGCC	540	0.0	11.08324	15
TCGACGC	140	8.367351E-9	10.856894	14
GATAACG	280	0.0	10.856421	11
CGAGCCG	690	0.0	10.73904	15
GGAGCGT	310	0.0	10.726339	6
AGGCCCG	710	0.0	10.703359	10
CGCAGAT	160	5.456968E-10	10.6867895	8
CGCTTAC	125	2.2439781E-7	10.639602	18
>>END_MODULE