FastQCFastQC Report
Thu 2 Feb 2017
SRR4050219_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4050219_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3465111
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCAAAGCAGGCCCGAGCCGCCTG40960.11820689149640516No Hit
CTATTGGAGCTGGAATTACCGCGGC40860.11791830045271277No Hit
GAATAGGACCGCGGTTCTATTTTGT37900.10937600555941786No Hit
GATTAAGAGGGACGGCCGGGGGCAT37210.10738472735794034No Hit
CTCTTAATCATGGCCTCAGTTCCGA36920.1065478133312324No Hit
GTATCAACGCAGAGTACTTTTTTTT36720.1059706312438476No Hit
GTCCTATTCCATTATTCCTAGCTGC36540.10545116736520128No Hit
CTTTAATATACGCTATTGGAGCTGG35360.10204579304963102No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGCGA350.002173184216.28317810
GGTATCA15400.015.7765771
GTATCAA51050.013.2512211
CGCATCG4700.012.73205913
GCTCGTA3500.011.9409979
TAGTACG1701.8189894E-1211.7361772
GCATCGC5100.011.73329614
CGAACGA2450.011.62899516
CCGCATC5350.011.54025812
CGTCGTA5000.011.39822510
ACGCGAA752.0782223E-411.39822411
AACCGCG4750.011.3975667
CCGTCGT5200.011.3251599
CGCCAGT5200.011.32238118
CGACCAT4800.011.27949310
ATCGCCA5500.011.05106916
TCGCCAG5600.011.02252217
CCGACCA5000.010.8283149
CATCGCC5800.010.80834915
GAACCGC5100.010.8008366
Produced by FastQC (version 0.11.5)