##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4050219_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3465111
Sequences flagged as poor quality	0
Sequence length	25
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.845988194894765	31.0	31.0	34.0	27.0	34.0
2	30.787303783342004	31.0	31.0	34.0	27.0	34.0
3	31.033551306148635	31.0	31.0	34.0	27.0	34.0
4	34.67671396385282	37.0	35.0	37.0	32.0	37.0
5	34.59776988385076	37.0	35.0	37.0	32.0	37.0
6	34.56028161868408	37.0	35.0	37.0	31.0	37.0
7	34.52956831685911	37.0	35.0	37.0	31.0	37.0
8	34.514715978795486	37.0	35.0	37.0	31.0	37.0
9	36.118209200224754	39.0	37.0	39.0	32.0	39.0
10	36.00297797097986	39.0	37.0	39.0	31.0	39.0
11	35.98924219166428	39.0	37.0	39.0	31.0	39.0
12	35.917903928618735	39.0	37.0	39.0	31.0	39.0
13	35.914772138612584	39.0	37.0	39.0	31.0	39.0
14	37.080960754215376	40.0	37.0	41.0	31.0	41.0
15	37.05150368920361	40.0	37.0	41.0	31.0	41.0
16	37.03297094955977	40.0	37.0	41.0	31.0	41.0
17	37.008207529282615	40.0	37.0	41.0	31.0	41.0
18	36.96748877597283	40.0	37.0	41.0	31.0	41.0
19	37.02922128612907	40.0	37.0	41.0	31.0	41.0
20	36.97880327643184	40.0	37.0	41.0	31.0	41.0
21	36.92688632485366	40.0	37.0	41.0	31.0	41.0
22	36.86226501835006	40.0	37.0	41.0	31.0	41.0
23	36.762523624784315	40.0	37.0	41.0	31.0	41.0
24	36.70825638774631	40.0	37.0	41.0	31.0	41.0
25	36.48780977001891	40.0	37.0	41.0	30.0	41.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.6789877448537815
1101	2	0.8315602943239675
1101	3	0.7714729794746837
1101	4	0.6797975529740228
1101	5	0.722409174780978
1101	6	0.7666707169901699
1101	7	0.7492825184926488
1101	8	0.8354937378525094
1101	9	0.8785072999158672
1101	10	0.8697448290895764
1101	11	0.854001108068303
1101	12	0.8817467279592179
1101	13	0.8653873426366019
1101	14	1.0877127807552256
1101	15	1.0487365252434486
1101	16	1.1100566210494307
1101	17	1.1196904266017853
1101	18	1.1677872388010897
1101	19	1.0340590496062632
1101	20	1.1497538428327232
1101	21	1.1522090437962618
1101	22	1.1561502250876643
1101	23	1.1842147513471488
1101	24	1.2378878894157026
1101	25	1.2095386281705274
1102	1	0.6830001470570828
1102	2	0.8841889748673388
1102	3	0.7791193399454137
1102	4	0.706047858738792
1102	5	0.7642768564372631
1102	6	0.8222699583093274
1102	7	0.8100160465762656
1102	8	0.832795323731105
1102	9	0.9615626167650078
1102	10	0.9903128407797155
1102	11	1.0616502860573362
1102	12	1.006727357520873
1102	13	1.0262215329650815
1102	14	1.1950025970914524
1102	15	1.2215547735410865
1102	16	1.196781115144809
1102	17	1.1343383200586103
1102	18	1.2041697245950331
1102	19	1.140074579254879
1102	20	1.2328552645268402
1102	21	1.1858911944335944
1102	22	1.2148903589710116
1102	23	1.2562004229858843
1102	24	1.3652896636838179
1102	25	1.3862815513969977
1103	1	0.6099230716482502
1103	2	0.6746684687507631
1103	3	0.649754540188102
1103	4	0.5149754119050556
1103	5	0.684996120611089
1103	6	0.6474798500596108
1103	7	0.6407965033423579
1103	8	0.5860427373448474
1103	9	0.741704867360788
1103	10	0.7823195984094156
1103	11	0.8448906483667429
1103	12	0.8903158920343017
1103	13	0.8694445065539114
1103	14	1.0172163330738044
1103	15	1.0128511256628556
1103	16	1.03721106656743
1103	17	1.0264465320695564
1103	18	1.0691853275732157
1103	19	1.0218788414803797
1103	20	1.0615863116602355
1103	21	1.0023200763132678
1103	22	0.985754359078939
1103	23	1.0180056502905686
1103	24	1.079113679246845
1103	25	1.1509959389807491
1104	1	0.49341590209270336
1104	2	0.6539549022012281
1104	3	0.6162277374347838
1104	4	0.5454147651644234
1104	5	0.5098835924790563
1104	6	0.6318238801460865
1104	7	0.6435468619846532
1104	8	0.5679957913978342
1104	9	0.6642233259227979
1104	10	0.6031688896796084
1104	11	0.714678325549194
1104	12	0.6985543003205805
1104	13	0.755455318467412
1104	14	0.8357711362482831
1104	15	0.9066575542436865
1104	16	0.8806023420178661
1104	17	0.80548981262654
1104	18	0.7890927715394724
1104	19	0.8266259986644116
1104	20	0.8486297007969981
1104	21	0.7665654139634697
1104	22	0.8052629368812845
1104	23	0.8860121255936022
1104	24	0.8309672676439419
1104	25	0.8904589297284389
1105	1	0.4587506185056718
1105	2	0.5687975430716321
1105	3	0.5017061970437631
1105	4	0.46388269808903715
1105	5	0.5816530112991245
1105	6	0.6732284540174973
1105	7	0.6090219156207652
1105	8	0.6529339109188328
1105	9	0.6322716398070725
1105	10	0.7021798842873466
1105	11	0.6644371221903285
1105	12	0.7495072565140433
1105	13	0.7300137356768062
1105	14	0.868567710539871
1105	15	0.8591492860058167
1105	16	0.9075533755873906
1105	17	0.8699913787374314
1105	18	0.8507511460946233
1105	19	0.8187595143923687
1105	20	0.8366668179433674
1105	21	0.7728004133586666
1105	22	0.832350726627709
1105	23	0.8594901013634484
1105	24	0.9118480617767588
1105	25	0.8825466478539425
1106	1	0.3467576943457864
1106	2	0.4610899750621087
1106	3	0.46143261973643845
1106	4	0.3736234461956869
1106	5	0.4858177063163964
1106	6	0.47141240300073406
1106	7	0.43750937288264424
1106	8	0.503688281122578
1106	9	0.5769739798024816
1106	10	0.5541964149006802
1106	11	0.49173804656052766
1106	12	0.5333453701923005
1106	13	0.5013530018650982
1106	14	0.5862179540338133
1106	15	0.6733151392480465
1106	16	0.6570797987372572
1106	17	0.6204060537524612
1106	18	0.5813474109715315
1106	19	0.5794602557325419
1106	20	0.5894201167890287
1106	21	0.6317323089690703
1106	22	0.688077577558829
1106	23	0.686161583807575
1106	24	0.6183956522497454
1106	25	0.6047462008705509
1107	1	0.19760970235282116
1107	2	0.37731368234203444
1107	3	0.31994385669742087
1107	4	0.301251731575249
1107	5	0.29592234734096934
1107	6	0.2661003252866152
1107	7	0.30713197674715786
1107	8	0.33135986850455623
1107	9	0.353715030904695
1107	10	0.3401960553296206
1107	11	0.3699350681135982
1107	12	0.43471861006639045
1107	13	0.409778146406083
1107	14	0.440451318573821
1107	15	0.4125705786738436
1107	16	0.43079979520001643
1107	17	0.3956262362128484
1107	18	0.45568624238021016
1107	19	0.43471894591457527
1107	20	0.47750110359233133
1107	21	0.5006630674228276
1107	22	0.48447024669425787
1107	23	0.43065623664976016
1107	24	0.45338129498667
1107	25	0.4715282299183343
1108	1	0.14305149148070484
1108	2	0.2669236993070463
1108	3	0.1564086237778639
1108	4	0.21697566356659337
1108	5	0.2879737341733559
1108	6	0.32207599048675206
1108	7	0.3420711309023474
1108	8	0.3390373952567387
1108	9	0.36890376005002423
1108	10	0.3868016904031535
1108	11	0.34091965807859026
1108	12	0.3523732632388814
1108	13	0.3972076549160164
1108	14	0.43951171508457776
1108	15	0.45484077703536485
1108	16	0.4241620345110846
1108	17	0.44744715359729526
1108	18	0.5117374806139381
1108	19	0.3862570658938367
1108	20	0.37763972491517706
1108	21	0.46668449747266294
1108	22	0.44931742889213666
1108	23	0.3988429968628182
1108	24	0.40015403279317496
1108	25	0.33913619834465436
1109	1	0.010866141524232376
1109	2	0.09709665574084525
1109	3	0.09604911203252087
1109	4	0.18100879537783499
1109	5	0.15781805106154678
1109	6	0.18027235347376802
1109	7	0.22767874490026685
1109	8	0.22232200500157262
1109	9	0.34090260620585155
1109	10	0.3635189693913361
1109	11	0.3256931388933495
1109	12	0.33308837190112683
1109	13	0.34589946691496465
1109	14	0.3267319293355868
1109	15	0.38556146604516783
1109	16	0.35323358486900247
1109	17	0.3724352975050138
1109	18	0.3118121714197599
1109	19	0.3262094011405168
1109	20	0.4119881206081786
1109	21	0.4018790455589425
1109	22	0.3962073209081183
1109	23	0.3755666288620816
1109	24	0.45263945091573987
1109	25	0.35268305008426637
1110	1	-0.2343024701069183
1110	2	-0.11855197340754842
1110	3	-0.14285666066296798
1110	4	-0.11486212133073792
1110	5	-0.009939647078617497
1110	6	-0.24887535494244162
1110	7	-0.11811445698629797
1110	8	-0.06358473916997553
1110	9	-0.0784842315171872
1110	10	-0.10798392072548069
1110	11	-0.07223151803070493
1110	12	-0.0917202943049844
1110	13	-0.12680489467646083
1110	14	-0.11789370647216657
1110	15	-0.15436956143843616
1110	16	-0.04559444363502507
1110	17	-0.1645260872187606
1110	18	-0.196811514256666
1110	19	-0.1915424394264562
1110	20	-0.09492978754311565
1110	21	-0.14356035238042608
1110	22	-0.2513279787206173
1110	23	-0.13851660872141736
1110	24	-0.18158000216640602
1110	25	-0.08230434879101267
1111	1	-0.4701201941639823
1111	2	-0.3317726292805716
1111	3	-0.3957957229757767
1111	4	-0.25858946115953785
1111	5	-0.3629256191821497
1111	6	-0.5577213587705998
1111	7	-0.4121845508150699
1111	8	-0.4163366219193989
1111	9	-0.3897485560851379
1111	10	-0.44507157952860865
1111	11	-0.5526560715527538
1111	12	-0.39571213059196
1111	13	-0.47005572172578525
1111	14	-0.5225887913004499
1111	15	-0.522004900474272
1111	16	-0.5752562917582225
1111	17	-0.6561778603077357
1111	18	-0.573489167394456
1111	19	-0.8009647810492311
1111	20	-0.673466857767778
1111	21	-0.6858453157243005
1111	22	-0.6721618086767691
1111	23	-0.7077432651257354
1111	24	-0.7382042866082514
1111	25	-0.608046762871183
1112	1	-0.4396057271562164
1112	2	-0.3737685346731219
1112	3	-0.3792804875214735
1112	4	-0.34111395498960206
1112	5	-0.41636893753832993
1112	6	-1.392517115460329
1112	7	-0.6888204937975004
1112	8	-0.427147260859023
1112	9	-0.5530900835807415
1112	10	-0.5045368987807706
1112	11	-0.6135559142937126
1112	12	-0.6171089756199777
1112	13	-0.536542215965504
1112	14	-0.603959530030771
1112	15	-0.6073137297161395
1112	16	-0.574281551438915
1112	17	-0.6551097260738104
1112	18	-0.6248546178116925
1112	19	-0.6461742232091936
1112	20	-0.6486032212428157
1112	21	-0.6335907751858443
1112	22	-0.7706630563390959
1112	23	-0.6712608859087226
1112	24	-0.7303700213841466
1112	25	-0.626487455843467
1113	1	-0.7035451709794991
1113	2	-0.7274335580802571
1113	3	-0.6246608728592946
1113	4	-0.5802753597210568
1113	5	-0.6600191940048106
1113	6	-1.1940758270614893
1113	7	-0.822203254542984
1113	8	-0.8413833439938898
1113	9	-0.8520135278845729
1113	10	-0.8889471450590278
1113	11	-0.8962986448393906
1113	12	-0.9199483463301164
1113	13	-0.9006628512718322
1113	14	-1.0896778969741447
1113	15	-0.9430097057763547
1113	16	-1.036427770330846
1113	17	-1.0280289603776254
1113	18	-1.0531792361111698
1113	19	-1.077076934885362
1113	20	-1.0211508321227285
1113	21	-1.078002121168943
1113	22	-1.0820763688283037
1113	23	-1.116179129743898
1113	24	-1.1037810728417767
1113	25	-1.0781014449815842
1114	1	-0.8432862662438403
1114	2	-0.8328604658605485
1114	3	-0.8935478889705699
1114	4	-0.7833256220826144
1114	5	-0.9880973939919855
1114	6	-1.4783407843668286
1114	7	-1.1591400131528644
1114	8	-1.3169504898821884
1114	9	-1.3089381092954326
1114	10	-1.418337112756788
1114	11	-1.2357262418836328
1114	12	-1.2613700453682384
1114	13	-1.3882998803347846
1114	14	-1.5749844811413425
1114	15	-1.5148039397377815
1114	16	-1.4999484358570996
1114	17	-1.4436581875451893
1114	18	-1.7434378443743626
1114	19	-1.4772939317320066
1114	20	-1.4098673221340832
1114	21	-1.4547687261345246
1114	22	-1.3378286599760898
1114	23	-1.5531451183838385
1114	24	-1.6492925114706907
1114	25	-1.6773368720543118
1115	1	-1.3348185295098496
1115	2	-1.9833273127375328
1115	3	-1.5316688038796897
1115	4	-1.305138322639614
1115	5	-1.3978682619283944
1115	6	-1.96849956301417
1115	7	-1.5913558894384963
1115	8	-1.5978583141740188
1115	9	-1.8021008503752896
1115	10	-1.7461001287782167
1115	11	-1.8556414519219118
1115	12	-1.7545669261758832
1115	13	-1.8801061788587887
1115	14	-1.9441160899031757
1115	15	-1.9451494148782729
1115	16	-2.001020695760296
1115	17	-2.010747034018273
1115	18	-2.0686429107099684
1115	19	-2.028598686517576
1115	20	-2.025734194321167
1115	21	-2.225855234647881
1115	22	-2.1714664058822137
1115	23	-2.1403484919672024
1115	24	-2.1932456873327695
1115	25	-2.2549923812945067
1116	1	-1.0182503330349917
1116	2	-1.0481149060691628
1116	3	-1.07631526910777
1116	4	-0.9775065852789027
1116	5	-1.030913260023027
1116	6	-1.1865446815029443
1116	7	-1.191240441928251
1116	8	-1.2352937986190895
1116	9	-1.3457831155857818
1116	10	-1.4206406942747236
1116	11	-1.538727148470052
1116	12	-1.4430486880341817
1116	13	-1.531144133372834
1116	14	-1.6541141164861983
1116	15	-1.5696021711712262
1116	16	-1.5885488313471257
1116	17	-1.6347257253025802
1116	18	-1.6924494645481332
1116	19	-1.6577756041344855
1116	20	-1.568915358187013
1116	21	-1.7564541379096212
1116	22	-1.6381760392977682
1116	23	-1.6277322854504277
1116	24	-1.7586407476135903
1116	25	-2.0299963333629805
1201	1	0.6141701792317953
1201	2	0.830005973403221
1201	3	0.745771021245794
1201	4	0.6399976656712667
1201	5	0.7828419295887272
1201	6	0.7979487729561683
1201	7	0.8304088641179916
1201	8	0.8274743429570535
1201	9	0.9371872403939605
1201	10	0.9509198186588748
1201	11	1.020079041542978
1201	12	1.0159831192445736
1201	13	0.9494444042681494
1201	14	1.105827589604111
1201	15	1.1149357094616548
1201	16	1.0395075582452051
1201	17	1.105316304396446
1201	18	1.162912723853033
1201	19	1.2302952489275967
1201	20	1.126087094983994
1201	21	1.175353767038338
1201	22	1.1569582369084728
1201	23	1.1295964169549677
1201	24	1.1430625603508489
1201	25	1.163830950197358
1202	1	0.6635863778026447
1202	2	0.7862931377941393
1202	3	0.7259153157433822
1202	4	0.7319818345771694
1202	5	0.733678063299152
1202	6	0.7785977773916528
1202	7	0.8295459612240847
1202	8	0.8114884735648076
1202	9	0.9428707289555831
1202	10	0.9790105589988869
1202	11	1.008372747606316
1202	12	0.9750426717048413
1202	13	1.0649845017552622
1202	14	1.184607851384449
1202	15	1.2496552837175159
1202	16	1.2020315544280464
1202	17	1.2056707266614737
1202	18	1.2106928236887669
1202	19	1.2628748811794992
1202	20	1.1750464092282513
1202	21	1.2386883982577217
1202	22	1.2337037321919055
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2211	3	-0.21593835346207157
2211	4	-0.1657404010610719
2211	5	-0.23392389103299394
2211	6	-0.2230706305991248
2211	7	-0.23051209086204238
2211	8	-0.22626819532207776
2211	9	-0.2666226070486246
2211	10	-0.35149708905429833
2211	11	-0.32419342733339107
2211	12	-0.3679297276752891
2211	13	-0.3652157524644366
2211	14	-0.4326690136014193
2211	15	-0.4546027647477473
2211	16	-0.44412790608657815
2211	17	-0.46308493276356444
2211	18	-0.3565323125827433
2211	19	-0.39314917070164057
2211	20	-0.49777856112592644
2211	21	-0.3812036649543842
2211	22	-0.44763563416272945
2211	23	-0.4070360147446834
2211	24	-0.5200820167564331
2211	25	-0.4608573024937357
2212	1	-0.27848794152090406
2212	2	-0.3886753739269224
2212	3	-0.3537988461604442
2212	4	-0.32546594714384014
2212	5	-0.38212275345993163
2212	6	-0.280659436308774
2212	7	-0.369589314270641
2212	8	-0.3657214813344467
2212	9	-0.36100150634737815
2212	10	-0.44083411542246154
2212	11	-0.4023195582205048
2212	12	-0.46398064912655457
2212	13	-0.44561745068460823
2212	14	-0.5349681271164428
2212	15	-0.4548476592781725
2212	16	-0.39823693730802034
2212	17	-0.4759997080431688
2212	18	-0.5048618684812212
2212	19	-0.521586385273153
2212	20	-0.4183863112784536
2212	21	-0.4359453047842976
2212	22	-0.5040576610019372
2212	23	-0.5274811129267434
2212	24	-0.5914296177273712
2212	25	-0.5976486953052742
2213	1	-0.531756154992248
2213	2	-0.7764474791532727
2213	3	-0.6296935596434139
2213	4	-0.5401387730892537
2213	5	-0.6339730195797202
2213	6	-0.4469328754695212
2213	7	-0.5825931481487743
2213	8	-0.6019543859370771
2213	9	-0.7067233047139183
2213	10	-0.7488746840410485
2213	11	-0.7035935035337317
2213	12	-0.7792387245063068
2213	13	-0.8020075256467578
2213	14	-0.78428670871034
2213	15	-0.9170937124342444
2213	16	-0.901860502096298
2213	17	-1.0058152878632427
2213	18	-1.1022325957971262
2213	19	-0.9303185630650219
2213	20	-0.9760145738187802
2213	21	-0.9188129005104386
2213	22	-0.8772579633045012
2213	23	-0.8236652937612376
2213	24	-0.9122074494646526
2213	25	-0.9651207865357065
2214	1	-0.7067653756006429
2214	2	-0.7415170352091351
2214	3	-0.7511214074161288
2214	4	-0.7119982215427711
2214	5	-0.7880791405731529
2214	6	-0.72542923435185
2214	7	-0.7449589281987912
2214	8	-0.7548133573060625
2214	9	-0.8364857307375217
2214	10	-0.8174948943818734
2214	11	-0.8911875797957549
2214	12	-0.9089164811936428
2214	13	-0.8428608726407489
2214	14	-1.0756725031121235
2214	15	-1.184775034535562
2214	16	-1.1740429155971341
2214	17	-1.0315884993369835
2214	18	-1.1112248323306417
2214	19	-1.0409466997109078
2214	20	-1.100165036664741
2214	21	-1.1920497582436553
2214	22	-1.0945300988444728
2214	23	-1.197496019193089
2214	24	-1.287067655403341
2214	25	-1.203432747819349
2215	1	-1.4265604802017577
2215	2	-3.267916125297983
2215	3	-1.7398213287219555
2215	4	-1.3182075851435968
2215	5	-1.2963694632907803
2215	6	-1.2670714930109455
2215	7	-1.383892864652971
2215	8	-1.3795886564992585
2215	9	-1.6075812895119626
2215	10	-1.7178042847355854
2215	11	-1.6936856560238382
2215	12	-1.6653293522614732
2215	13	-1.670828783923966
2215	14	-1.9182694063477896
2215	15	-2.0204528962175345
2215	16	-1.9740412058259835
2215	17	-2.0328424432649825
2215	18	-1.9119194352424387
2215	19	-1.8479671765113252
2215	20	-1.8696639709073892
2215	21	-1.8860478804008807
2215	22	-1.947858498053911
2215	23	-2.113754277778881
2215	24	-2.2227562897610653
2215	25	-2.225210650558182
2216	1	-0.9885600086189754
2216	2	-1.7300768866929879
2216	3	-1.15776146122235
2216	4	-0.9190703464981098
2216	5	-1.0271323801631027
2216	6	-0.9675942183588262
2216	7	-1.1688647776091514
2216	8	-1.1880236635305437
2216	9	-1.3819456379406603
2216	10	-1.3061540454497802
2216	11	-1.368406260642601
2216	12	-1.3577192885194194
2216	13	-1.543130654383262
2216	14	-1.599491964029049
2216	15	-1.6119623813235933
2216	16	-1.531253330772337
2216	17	-1.5617879049120873
2216	18	-1.7463897961774393
2216	19	-1.6232852499541366
2216	20	-1.723978964257114
2216	21	-1.5923159797850985
2216	22	-1.6339249032671859
2216	23	-1.734397824987127
2216	24	-1.6838947441582945
2216	25	-1.8480482225121122
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	44384.0
3	3.0
4	879.0
5	7164.0
6	8744.0
7	6643.0
8	5343.0
9	4722.0
10	4269.0
11	4029.0
12	3730.0
13	3737.0
14	3644.0
15	3768.0
16	3983.0
17	4340.0
18	4615.0
19	5372.0
20	6960.0
21	8967.0
22	11593.0
23	14384.0
24	17279.0
25	20615.0
26	23556.0
27	26807.0
28	31701.0
29	38800.0
30	50096.0
31	65594.0
32	85216.0
33	113897.0
34	154524.0
35	212611.0
36	309125.0
37	534640.0
38	1619377.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.216024294079276	19.35798355435838	13.179908625029352	26.246083526532992
2	15.470587080805002	21.332505564637536	38.06347727503625	25.13343007952121
3	17.310902883053387	25.976570447526786	29.59276052051435	27.119766148905477
4	12.332649661150825	17.949295130805336	37.528523617280946	32.18953159076289
5	13.940886087900811	37.32612727274302	34.02178391577479	14.71120272358138
6	30.638543980273635	38.29009918591819	18.182382262898095	12.888974570910081
7	27.85159116863672	31.98057471798449	22.22689438202766	17.940939731351133
8	26.3325399924331	33.58536120098364	20.675895279001168	19.40620352758209
9	27.12513063573605	15.154033999781227	19.863212370630574	37.85762299385215
10	15.261808303190328	27.801423311693483	32.34140430803476	24.595364077081424
11	34.9361538541461	22.715859541942432	23.4565175480217	18.891469055889765
12	23.83143542817231	25.24918573591146	29.535945231810924	21.383433604105306
13	28.463188156478115	21.016045559112257	25.648100308557343	24.872665975852286
14	22.994254538821977	21.363054964565617	26.5043884610545	29.138302035557906
15	24.146958823160958	28.044950942428976	23.2519406684969	24.556149565913163
16	24.469149620566554	27.399155672914606	24.441066956517474	23.69062775000137
17	23.086500420790394	27.126145917871412	25.865506385739533	23.921847275598658
18	23.48317596422847	26.527905960632616	27.045379905122562	22.94353817001635
19	25.045989172702836	26.16733420953746	26.332445007800985	22.45423160995871
20	25.09282940803323	25.624293281477005	25.76158210287109	23.521295207618678
21	26.388017884977078	25.40262987546143	25.08266159812134	23.12669064144015
22	24.930485632350592	25.583682600643964	25.629453140173574	23.856378626831866
23	23.672169867134734	25.231066887464237	26.084887391508378	25.01187585389265
24	23.753234140984738	26.471370005930762	25.937255139632637	23.83814071345186
25	23.894562559469165	25.796710043863964	26.08842460572503	24.22030279094184
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	295.0
1	295.0
2	563.0
3	831.0
4	831.0
5	831.0
6	2255.0
7	3679.0
8	3679.0
9	3679.0
10	5699.5
11	7720.0
12	7720.0
13	7720.0
14	12939.0
15	18158.0
16	18158.0
17	18158.0
18	29910.5
19	41663.0
20	41663.0
21	41663.0
22	63221.0
23	84779.0
24	84779.0
25	84779.0
26	116513.0
27	148247.0
28	148247.0
29	148247.0
30	175394.0
31	202541.0
32	202541.0
33	202541.0
34	242149.5
35	281758.0
36	281758.0
37	281758.0
38	306822.0
39	331886.0
40	331886.0
41	331886.0
42	360234.0
43	388582.0
44	388582.0
45	388582.0
46	414244.0
47	439906.0
48	439906.0
49	439906.0
50	432574.5
51	425243.0
52	425243.0
53	425243.0
54	382225.5
55	339208.0
56	339208.0
57	339208.0
58	300321.0
59	261434.0
60	261434.0
61	261434.0
62	230240.0
63	199046.0
64	199046.0
65	199046.0
66	164401.0
67	129756.0
68	129756.0
69	129756.0
70	98913.5
71	68071.0
72	68071.0
73	68071.0
74	54408.0
75	40745.0
76	40745.0
77	40745.0
78	34762.0
79	28779.0
80	28779.0
81	28779.0
82	20944.5
83	13110.0
84	13110.0
85	13110.0
86	9916.5
87	6723.0
88	6723.0
89	6723.0
90	4694.5
91	2666.0
92	2666.0
93	2666.0
94	1445.5
95	225.0
96	225.0
97	225.0
98	142.5
99	60.0
100	60.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.32985956294040797
2	0.010822164138464829
3	0.0
4	0.0
5	0.049926250558784406
6	0.002972487750031673
7	0.004097992820432015
8	5.194638786463118E-4
9	0.009350349815633612
10	0.014862438750158365
11	0.00632014385686346
12	0.009408068024372091
13	0.017344321725912965
14	0.0033187970024625476
15	0.020547682310898555
16	0.009812095485541444
17	0.005858398186955627
18	2.597319393231559E-4
19	0.0047617522209245245
20	0.00441544296849365
21	0.002308728349539163
22	0.0
23	0.0027993331238162357
24	0.003636247150524182
25	0.002308728349539163
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	3465111.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	65.17425551026366
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.59178059319353	59.69426111020803
2	5.736320386029313	7.477208210556174
3	1.0864298056665667	2.124217612454367
4	0.4493109642381803	1.1713403034728838
5	0.2435685568247328	0.7937199678380653
6	0.15252642720566617	0.5964477803261831
7	0.10566756023392544	0.4820763198882423
8	0.08156774947796337	0.4252893876699168
9	0.06012942504587636	0.3527001460462673
>10	0.3628697638802572	4.583610077359235
>50	0.05243369560954243	2.435830812812757
>100	0.0610894746737622	8.563093418454
>500	0.01052977304661337	4.691163002237724
>1k	0.005775824874176398	6.60904185067635
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTTCAAAGCAGGCCCGAGCCGCCTG	4096	0.11820689149640516	No Hit
CTATTGGAGCTGGAATTACCGCGGC	4086	0.11791830045271277	No Hit
GAATAGGACCGCGGTTCTATTTTGT	3790	0.10937600555941786	No Hit
GATTAAGAGGGACGGCCGGGGGCAT	3721	0.10738472735794034	No Hit
CTCTTAATCATGGCCTCAGTTCCGA	3692	0.1065478133312324	No Hit
GTATCAACGCAGAGTACTTTTTTTT	3672	0.1059706312438476	No Hit
GTCCTATTCCATTATTCCTAGCTGC	3654	0.10545116736520128	No Hit
CTTTAATATACGCTATTGGAGCTGG	3536	0.10204579304963102	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	2.885910436923954E-5	0.0	0.0	0.0
4	0.0	2.885910436923954E-5	0.0	2.885910436923954E-5	0.0
5	0.0	2.885910436923954E-5	0.0	5.771820873847908E-5	0.0
6	0.0	2.885910436923954E-5	0.0	5.771820873847908E-5	0.0
7	0.0	2.885910436923954E-5	0.0	8.657731310771862E-5	0.0
8	0.0	2.885910436923954E-5	0.0	8.657731310771862E-5	0.0
9	0.0	2.885910436923954E-5	0.0	1.4429552184619772E-4	0.0
10	0.0	2.885910436923954E-5	0.0	1.7315462621543724E-4	0.0
11	0.0	2.885910436923954E-5	0.0	1.7315462621543724E-4	0.0
12	0.0	2.885910436923954E-5	0.0	2.0201373058467679E-4	0.0
13	0.0	2.885910436923954E-5	0.0	2.0201373058467679E-4	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCGCGA	35	0.0021731842	16.283178	10
GGTATCA	1540	0.0	15.776577	1
GTATCAA	5105	0.0	13.251221	1
CGCATCG	470	0.0	12.732059	13
GCTCGTA	350	0.0	11.940997	9
TAGTACG	170	1.8189894E-12	11.736177	2
GCATCGC	510	0.0	11.733296	14
CGAACGA	245	0.0	11.628995	16
CCGCATC	535	0.0	11.540258	12
CGTCGTA	500	0.0	11.398225	10
ACGCGAA	75	2.0782223E-4	11.398224	11
AACCGCG	475	0.0	11.397566	7
CCGTCGT	520	0.0	11.325159	9
CGCCAGT	520	0.0	11.322381	18
CGACCAT	480	0.0	11.279493	10
ATCGCCA	550	0.0	11.051069	16
TCGCCAG	560	0.0	11.022522	17
CCGACCA	500	0.0	10.828314	9
CATCGCC	580	0.0	10.808349	15
GAACCGC	510	0.0	10.800836	6
>>END_MODULE