FastQCFastQC Report
Thu 2 Feb 2017
SRR4050218_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4050218_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3431205
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNN113670.33128303322010777No Hit
GNNNNNNNNNNNNNNNNNNNNNNNN52340.15254116265277068No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG41120.11984128024994135No Hit
GTCCTATTCCATTATTCCTAGCTGC41000.11949154888734423No Hit
CTATTGGAGCTGGAATTACCGCGGC39010.11369183712427557No Hit
GTATCTGATCGTCTTCGAACCTCCG38390.11188489175085721No Hit
GAATAGGACCGCGGTTCTATTTTGT38370.11182660319042435No Hit
CTCTTAATCATGGCCTCAGTTCCGA35010.10203412503770541No Hit
GATTAAGAGGGACGGCCGGGGGCAT34590.10081006526861554No Hit
CTTTAATATACGCTATTGGAGCTGG34480.10048947818623487No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA14100.016.0457761
GTATCAA48050.013.2155711
CTCGACG1301.4551915E-1113.15322613
CGAACGA1850.012.83723316
TCGACGC1405.2750693E-1112.2137114
CGCATCG5300.012.18810513
GTGATCG550.003070820112.0903399
CCGCATC5550.011.81025412
ATCGCCA5500.011.74490116
CGACCAT4550.011.69175710
TCGCCAG5450.011.67834917
CGTCGTA4000.011.63695110
CGCCAGT5650.011.43325818
AGGCCCG7650.011.42429810
ACGAACG2100.011.30882515
CCGTCGT4400.011.2267449
CATCGCC5700.011.16597715
AACCGCG3500.011.128217
CGAGCCG7700.011.103209515
GCATCGC6100.011.05685514
Produced by FastQC (version 0.11.5)