##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4050218_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3431205
Sequences flagged as poor quality	0
Sequence length	25
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.967143029926802	31.0	31.0	34.0	26.0	34.0
2	30.026568217288094	31.0	31.0	34.0	26.0	34.0
3	30.175019271655295	31.0	31.0	34.0	26.0	34.0
4	33.63613132995551	37.0	35.0	37.0	28.0	37.0
5	33.65489674910126	37.0	35.0	37.0	28.0	37.0
6	33.72887717288824	37.0	35.0	37.0	29.0	37.0
7	33.677593148762604	37.0	35.0	37.0	29.0	37.0
8	33.620802021447275	37.0	35.0	37.0	29.0	37.0
9	35.15332543523339	39.0	35.0	39.0	29.0	39.0
10	35.02357830558069	39.0	35.0	39.0	28.0	39.0
11	35.07142855061123	39.0	35.0	39.0	28.0	39.0
12	34.99201330144949	39.0	35.0	39.0	28.0	39.0
13	34.99711559058698	39.0	35.0	39.0	28.0	39.0
14	36.06037325079673	40.0	36.0	41.0	27.0	41.0
15	36.03992270936887	40.0	36.0	41.0	27.0	41.0
16	36.038942587225186	40.0	36.0	41.0	27.0	41.0
17	36.00228840888259	40.0	36.0	41.0	27.0	41.0
18	35.937829712885126	40.0	36.0	41.0	27.0	41.0
19	35.98787656231557	40.0	37.0	41.0	27.0	41.0
20	35.95229256194253	40.0	37.0	41.0	27.0	41.0
21	35.87294929915292	40.0	37.0	41.0	27.0	41.0
22	35.77051240016262	40.0	36.0	41.0	27.0	41.0
23	35.691410452013216	40.0	36.0	41.0	27.0	41.0
24	35.62947594212529	40.0	36.0	41.0	27.0	41.0
25	35.29690181729159	40.0	36.0	41.0	25.0	41.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	1.236192895702473
1101	2	1.2268508714416164
1101	3	1.2251737936379783
1101	4	1.308583285052599
1101	5	1.2666370274243377
1101	6	1.2307415397132857
1101	7	1.2629693070097048
1101	8	1.2721530202178855
1101	9	1.395637324310563
1101	10	1.4435086008276627
1101	11	1.4334787039905876
1101	12	1.5667433615708788
1101	13	1.564990468423133
1101	14	1.782469574390916
1101	15	1.7478747751983121
1101	16	1.8342996606178232
1101	17	1.7501289499530799
1101	18	1.849158173269565
1101	19	1.7456666546656692
1101	20	1.8017444096770774
1101	21	1.8260405707329284
1101	22	1.7759689142205062
1101	23	1.8141923888550409
1101	24	1.8465223754349012
1101	25	1.8135018881454954
1102	1	1.1771328711507003
1102	2	1.2292414014549387
1102	3	1.2283808133563667
1102	4	1.3105215204891465
1102	5	1.2592293955574902
1102	6	1.1972716934620422
1102	7	1.1910996607042534
1102	8	1.1910910146653393
1102	9	1.4097307148635068
1102	10	1.4780755516039505
1102	11	1.4042384875247222
1102	12	1.4649550569344
1102	13	1.3886430582377116
1102	14	1.6835908349094524
1102	15	1.7413984191969973
1102	16	1.6976913809251002
1102	17	1.5687134429849934
1102	18	1.6651813127894997
1102	19	1.5746244296275265
1102	20	1.6008943165970635
1102	21	1.7039210825735935
1102	22	1.656008100398516
1102	23	1.6932329877638423
1102	24	1.758970694309994
1102	25	1.7431285053673804
1103	1	0.993680440655563
1103	2	1.0265434885577065
1103	3	1.010297137951806
1103	4	1.1164069010874442
1103	5	1.0228654844361955
1103	6	1.033726718006747
1103	7	0.996612226420794
1103	8	0.9836018423587163
1103	9	1.1838827623456396
1103	10	1.2033431073617251
1103	11	1.1876805027303234
1103	12	1.1229371276152165
1103	13	1.0846566808211051
1103	14	1.2994997960490196
1103	15	1.40078202951279
1103	16	1.326253335293103
1103	17	1.425790062144003
1103	18	1.4244456947343664
1103	19	1.4586201835193933
1103	20	1.4376199486427126
1103	21	1.4985850971872168
1103	22	1.4869739234991215
1103	23	1.4604952895464507
1103	24	1.4873064622511905
1103	25	1.5366679673249237
1104	1	0.9820266297708713
1104	2	1.0823073557954963
1104	3	1.0543008481361902
1104	4	1.1375695292799648
1104	5	1.0432682130540911
1104	6	1.0047043298038005
1104	7	0.9669589374104675
1104	8	0.9851914149698899
1104	9	1.1754936923800656
1104	10	1.1664357127408351
1104	11	1.1942705067783947
1104	12	1.1402601403554868
1104	13	1.176144041795105
1104	14	1.341241272490059
1104	15	1.3611033229035385
1104	16	1.3962861781025708
1104	17	1.3774827040785311
1104	18	1.378258083838226
1104	19	1.3986351515264843
1104	20	1.3728248707929893
1104	21	1.3983053289492346
1104	22	1.4835399797733686
1104	23	1.411690093074931
1104	24	1.3861645119039565
1104	25	1.4236046114652225
1105	1	0.7785356708538522
1105	2	0.9360727454205531
1105	3	0.9007086877992734
1105	4	0.9970061354487143
1105	5	0.9467234421949584
1105	6	0.9015329950668658
1105	7	0.779572899170546
1105	8	0.8083340472808445
1105	9	0.9320736790600606
1105	10	0.9387134917357756
1105	11	0.8551023532928994
1105	12	0.9396492254277717
1105	13	0.97889879271046
1105	14	1.0817247396851286
1105	15	1.02082514131029
1105	16	1.0747169573032593
1105	17	1.1589886161292497
1105	18	1.1444377504163228
1105	19	1.1425030637000901
1105	20	1.149452161126824
1105	21	1.112388214452352
1105	22	1.2071635721000789
1105	23	1.1889946677546916
1105	24	1.1309423885009906
1105	25	1.21784409519055
1106	1	0.7306524058153663
1106	2	0.8293824261496212
1106	3	0.7984006485472364
1106	4	0.7996966812925876
1106	5	0.8721780021990142
1106	6	0.7597856124433022
1106	7	0.735328334391042
1106	8	0.7127206971449169
1106	9	0.9139407044358379
1106	10	0.9003320902362049
1106	11	0.934371070293011
1106	12	0.9691153189987887
1106	13	0.8020438620776318
1106	14	1.087713702727143
1106	15	1.0927628350064182
1106	16	1.0710213392223906
1106	17	1.0659311154782856
1106	18	1.159768924664327
1106	19	1.1312689919623011
1106	20	1.1661810582069307
1106	21	1.2036623946219365
1106	22	1.1877042670874758
1106	23	1.2290529286959568
1106	24	1.1289019767690007
1106	25	1.1299809047658016
1107	1	0.6792402777285957
1107	2	0.731753443292213
1107	3	0.7596452997790841
1107	4	0.7900307571384602
1107	5	0.7137521162685587
1107	6	0.6273119602788313
1107	7	0.6070489128775094
1107	8	0.6599909474007077
1107	9	0.6690986994966011
1107	10	0.6949398436103991
1107	11	0.7037093646524752
1107	12	0.6600826166328488
1107	13	0.7021025952684994
1107	14	0.8117181856508182
1107	15	0.7284319458180377
1107	16	0.8115102235090745
1107	17	0.7483314140195958
1107	18	0.7455675175973795
1107	19	0.7405810532390831
1107	20	0.7405160387143326
1107	21	0.7838080915177628
1107	22	0.8075178183872893
1107	23	0.7062400813762437
1107	24	0.6806380588061529
1107	25	0.5963102800047579
1108	1	0.44361331542951987
1108	2	0.5647448725773643
1108	3	0.5359752438991379
1108	4	0.6422837599444833
1108	5	0.6164563688160598
1108	6	0.5250559418549727
1108	7	0.5080004152363813
1108	8	0.5339738343360878
1108	9	0.6222343900902629
1108	10	0.6248754906914371
1108	11	0.5652690027169314
1108	12	0.495325487913334
1108	13	0.5987976535810446
1108	14	0.6791384667882738
1108	15	0.6553675831759946
1108	16	0.6312006946465445
1108	17	0.6715720970946819
1108	18	0.6734700168610175
1108	19	0.6928606738984797
1108	20	0.7666548292169182
1108	21	0.716294366435811
1108	22	0.7354595755121309
1108	23	0.6884209235545953
1108	24	0.5687761461763188
1108	25	0.6336358064091812
1109	1	0.5352596538954444
1109	2	0.6658398399717278
1109	3	0.5429394382627564
1109	4	0.6243794542381877
1109	5	0.6300629583123012
1109	6	0.43240871782136736
1109	7	0.4624126630723637
1109	8	0.4969494975874724
1109	9	0.5040384463047616
1109	10	0.5670200548344582
1109	11	0.5564397469947053
1109	12	0.5768929866301775
1109	13	0.5521900323155791
1109	14	0.5711278263759283
1109	15	0.5944060728573817
1109	16	0.5884788185747354
1109	17	0.6619580705865857
1109	18	0.6540605577628114
1109	19	0.6755289909047946
1109	20	0.5285998990869416
1109	21	0.6606391788097881
1109	22	0.664456271668918
1109	23	0.6275402349226624
1109	24	0.5250597596041331
1109	25	0.5673532399090817
1110	1	-0.03051547442021274
1110	2	0.1604598194626412
1110	3	0.06715963783811674
1110	4	0.08207598402589866
1110	5	0.13629640847067037
1110	6	0.013354545454014044
1110	7	-0.10611305226902346
1110	8	-0.03387156197723584
1110	9	0.04227544137561523
1110	10	0.06269319998042278
1110	11	0.04270722724199061
1110	12	0.007002921756139813
1110	13	0.009994261578242458
1110	14	0.04817706156909196
1110	15	-0.07481195800263407
1110	16	0.008080591566461237
1110	17	0.04498077783946286
1110	18	0.0987341527163963
1110	19	-0.013165977943351947
1110	20	0.011756745399992496
1110	21	-0.11533908584309671
1110	22	-0.020863801264177084
1110	23	-0.025848985223369425
1110	24	0.04392430325020058
1110	25	0.1291904095481371
1111	1	-0.19970097049906954
1111	2	-0.08870886810665368
1111	3	-0.26826619461344237
1111	4	-0.17341766468638298
1111	5	-0.1762770654736059
1111	6	-0.20157591693187982
1111	7	-0.3599550877187738
1111	8	-0.25333348715911796
1111	9	-0.3056619722402871
1111	10	-0.24169954198364252
1111	11	-0.2909543445561624
1111	12	-0.3049944050833062
1111	13	-0.26132406729138324
1111	14	-0.3952709105247294
1111	15	-0.24058461784801466
1111	16	-0.3044411152060462
1111	17	-0.23001757674920498
1111	18	-0.27650911501986997
1111	19	-0.36128312110425753
1111	20	-0.41329780631402
1111	21	-0.2896420642907387
1111	22	-0.2547261132442884
1111	23	-0.3521231664413733
1111	24	-0.3357522088828091
1111	25	-0.29846465148396817
1112	1	-0.28455284891622057
1112	2	-0.12059154823440821
1112	3	-0.3059681785900601
1112	4	-0.225510584082258
1112	5	-0.17036049128424935
1112	6	-0.2488467649262489
1112	7	-0.42476843016451227
1112	8	-0.37199315261680965
1112	9	-0.5028306440856838
1112	10	-0.48095328393134906
1112	11	-0.34795730157451743
1112	12	-0.42279217074129605
1112	13	-0.3775037619809325
1112	14	-0.5384325518390796
1112	15	-0.6047859183947608
1112	16	-0.5391358385861196
1112	17	-0.4487768808687349
1112	18	-0.5044498934532555
1112	19	-0.5058716760222453
1112	20	-0.45153012510360213
1112	21	-0.43869913406825134
1112	22	-0.4275239127354382
1112	23	-0.4963869230050122
1112	24	-0.457567662517846
1112	25	-0.5614126025476835
1113	1	-0.5238854024051349
1113	2	-0.43617088203370713
1113	3	-0.46087367503707455
1113	4	-0.41036364327276686
1113	5	-0.40406647537671603
1113	6	-0.43368344253015323
1113	7	-0.42960423408379
1113	8	-0.47765398177587315
1113	9	-0.4512490726829981
1113	10	-0.4795365800521907
1113	11	-0.3583988439838137
1113	12	-0.379182567005806
1113	13	-0.45248450856061595
1113	14	-0.5748991770866283
1113	15	-0.5250033445198738
1113	16	-0.5498297787743169
1113	17	-0.656174441347126
1113	18	-0.5989178518788165
1113	19	-0.695122078887934
1113	20	-0.7334610732779225
1113	21	-0.7464484739509842
1113	22	-0.7458068150510258
1113	23	-0.7980352342050026
1113	24	-0.883865690902887
1113	25	-1.0342304943143361
1114	1	-0.710656919576742
1114	2	-0.6435348012179851
1114	3	-0.808410427201661
1114	4	-0.6944356106685703
1114	5	-0.8845963562257566
1114	6	-0.8307987175435301
1114	7	-0.771184816197831
1114	8	-0.8382639785650596
1114	9	-0.9439474909485028
1114	10	-0.8719150439455277
1114	11	-0.8777151445380014
1114	12	-1.1424841387195528
1114	13	-1.0404114188402005
1114	14	-1.0747287824901477
1114	15	-1.0194397793187804
1114	16	-1.0527882815732355
1114	17	-1.3943358581817407
1114	18	-1.1002787957337716
1114	19	-1.0884380679636436
1114	20	-1.0245114112905327
1114	21	-1.1000345056858833
1114	22	-1.1097930548836104
1114	23	-1.302059778375444
1114	24	-1.1848123600246367
1114	25	-1.29286569246851
1115	1	-1.386025752252145
1115	2	-1.3299608512710925
1115	3	-1.481088518428674
1115	4	-1.5117228434025805
1115	5	-1.4101777935292574
1115	6	-1.579751247505797
1115	7	-1.5394919935310796
1115	8	-1.680615132091404
1115	9	-2.2993667769052806
1115	10	-1.858317750677429
1115	11	-1.9147181081011055
1115	12	-1.9029831937797184
1115	13	-1.7992147756863304
1115	14	-2.1272044045732628
1115	15	-2.1677962619012234
1115	16	-2.166726595070216
1115	17	-2.144666737459602
1115	18	-1.9970996759245878
1115	19	-2.017711713463328
1115	20	-2.0645402870335445
1115	21	-2.1578885492466
1115	22	-2.300875878846
1115	23	-2.1563988480429614
1115	24	-2.401415010229613
1115	25	-2.2839033058298597
1116	1	-1.5927926990474646
1116	2	-1.8126814068082417
1116	3	-1.6097901665896437
1116	4	-1.9358823266497929
1116	5	-1.6020949861274474
1116	6	-1.7244366215813116
1116	7	-1.7231243477801854
1116	8	-1.7792820068636033
1116	9	-2.319017054970317
1116	10	-3.013957439774728
1116	11	-2.442888651169355
1116	12	-2.1418418453604815
1116	13	-2.1633047121560125
1116	14	-2.4509692429921017
1116	15	-3.227033095883577
1116	16	-2.7021033736313953
1116	17	-2.535330239526992
1116	18	-2.6675581649737623
1116	19	-2.4381135257253916
1116	20	-2.519387198671815
1116	21	-2.4139699685031033
1116	22	-2.490814179623605
1116	23	-2.539795078077468
1116	24	-2.4231427105297385
1116	25	-2.623486635530064
1201	1	1.104048673607057
1201	2	1.196149392122944
1201	3	1.0619681312832725
1201	4	1.1789280116731362
1201	5	1.077813162352193
1201	6	1.0538761085742934
1201	7	1.0238534870404266
1201	8	1.0458115617527852
1201	9	1.2188933618701938
1201	10	1.2935127319230233
1201	11	1.2298164275589443
1201	12	1.272153985202003
1201	13	1.3013476347139772
1201	14	1.5173073189771245
1201	15	1.4776161976189925
1201	16	1.4448538120304377
1201	17	1.4149061679526724
1201	18	1.4517869994248542
1201	19	1.3949889376007718
1201	20	1.4306979283315115
1201	21	1.4426263629538099
1201	22	1.5436902261790806
1201	23	1.5436692279051485
1201	24	1.5914359340764008
1201	25	1.5419082178532904
1202	1	1.0579972523758414
1202	2	1.0944724807162771
1202	3	1.0252130664778427
1202	4	1.052795100051405
1202	5	1.0511263451644055
1202	6	0.9955917781758785
1202	7	0.988427842346475
1202	8	1.0399681443927165
1202	9	1.0913786668338332
1202	10	1.2856311197410761
1202	11	1.1940616421023833
1202	12	1.2273700364245315
1202	13	1.2270674840292202
1202	14	1.4075496278247712
1202	15	1.346744173298113
1202	16	1.2557540283797124
1202	17	1.3128888950755808
1202	18	1.3349303404607369
1202	19	1.3289087866822484
1202	20	1.3648183475652047
1202	21	1.4007184218484952
1202	22	1.4904484351203493
1202	23	1.390785681319926
1202	24	1.4644404305412948
1202	25	1.5602440232488135
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2211	9	-0.4138740001188239
2211	10	-0.4952365293596799
2211	11	-0.5457128083941143
2211	12	-0.5338323516668879
2211	13	-0.4613757368157323
2211	14	-0.5246105722718042
2211	15	-0.5516241450959924
2211	16	-0.543347413507064
2211	17	-0.5559790449157944
2211	18	-0.6452566170513307
2211	19	-0.6586306573511678
2211	20	-0.6411476984908404
2211	21	-0.6055908148518725
2211	22	-0.5352949703384056
2211	23	-0.6550272176067011
2211	24	-0.5683091056633813
2211	25	-0.5741747239734494
2212	1	-0.5747508808428279
2212	2	-0.5583001552804383
2212	3	-0.5212822140708191
2212	4	-0.5409866163430124
2212	5	-0.5849012567558205
2212	6	-0.5433862678386845
2212	7	-0.5202389043171678
2212	8	-0.5858535838234786
2212	9	-0.6436127520088633
2212	10	-0.703506031373955
2212	11	-0.8182305601921982
2212	12	-0.823527374117738
2212	13	-0.7103831752421712
2212	14	-0.8498869060543228
2212	15	-0.897232943121935
2212	16	-0.947853168681732
2212	17	-1.009805358697058
2212	18	-0.9351363459088873
2212	19	-1.0021073749294231
2212	20	-1.0026812135624823
2212	21	-0.8745409572416278
2212	22	-0.8071880245393217
2212	23	-0.9326690992772484
2212	24	-0.8835989027875968
2212	25	-0.9421591452240818
2213	1	-1.035334421096799
2213	2	-0.8895122660375776
2213	3	-0.8641206359560911
2213	4	-0.8876849163724785
2213	5	-0.8058601668294543
2213	6	-0.8808176155436271
2213	7	-0.9898242697443536
2213	8	-1.0702744518914855
2213	9	-1.0085567405609552
2213	10	-1.1104801105815199
2213	11	-1.1033430237441806
2213	12	-1.102960477875662
2213	13	-1.176055238098634
2213	14	-1.268483130399595
2213	15	-1.202829258884293
2213	16	-1.3541363389136052
2213	17	-1.282859568324767
2213	18	-1.1890555747476839
2213	19	-1.219495972531476
2213	20	-1.316126724696062
2213	21	-1.3070864724473168
2213	22	-1.2271162216406637
2213	23	-1.3341348883398965
2213	24	-1.3596380328208326
2213	25	-1.2823496401237904
2214	1	-0.9897983233704117
2214	2	-1.0142735436939283
2214	3	-1.0146236816836343
2214	4	-1.0355755827940456
2214	5	-1.0201432104763697
2214	6	-1.0539904776100997
2214	7	-1.1384385680032238
2214	8	-1.0791367565494028
2214	9	-1.198556358016674
2214	10	-1.6233892490450685
2214	11	-1.5783421106577222
2214	12	-1.3732762188457528
2214	13	-1.3415815419990764
2214	14	-1.4583118146082512
2214	15	-1.5735707491348663
2214	16	-1.6271003768086842
2214	17	-1.6621530869341612
2214	18	-1.6261019417108145
2214	19	-1.650248360966593
2214	20	-1.6925734907169243
2214	21	-1.5609078907380223
2214	22	-1.450361793084788
2214	23	-1.7076675670551467
2214	24	-1.6638959237486262
2214	25	-1.5194619224089294
2215	1	-1.7560142549855549
2215	2	-1.4627295929893869
2215	3	-1.56897416647411
2215	4	-2.1382367502789066
2215	5	-1.7136941143686855
2215	6	-1.5558823511717534
2215	7	-1.5928298819068658
2215	8	-1.7584707320658772
2215	9	-1.7702444276716207
2215	10	-1.8176012038162028
2215	11	-1.8940039340940729
2215	12	-1.9315707415743262
2215	13	-1.9673906239100631
2215	14	-2.106579033504822
2215	15	-2.058984199998932
2215	16	-2.0830028098464766
2215	17	-2.0939685529410355
2215	18	-2.442786277762629
2215	19	-2.8640335580163594
2215	20	-2.4470982216518777
2215	21	-2.3696605511320996
2215	22	-3.2419830539360746
2215	23	-2.509876010726529
2215	24	-2.5467265677488484
2215	25	-2.3621988702236436
2216	1	-2.9862548523834924
2216	2	-5.826396954660044
2216	3	-3.452462984674561
2216	4	-5.946299178439553
2216	5	-3.6008809488800857
2216	6	-2.5652487551116394
2216	7	-2.380104164722571
2216	8	-2.2310968351937746
2216	9	-2.5620044934551416
2216	10	-2.5857084603706895
2216	11	-2.390821522277477
2216	12	-2.541345651293817
2216	13	-2.485488970156979
2216	14	-3.086532193927276
2216	15	-3.0508688987096093
2216	16	-3.0744573475478916
2216	17	-2.8198539192883274
2216	18	-2.7657884276925557
2216	19	-2.856210626194212
2216	20	-3.1601417663249762
2216	21	-3.344132997278834
2216	22	-3.2328228599714066
2216	23	-3.1094887146611896
2216	24	-3.0737539968578034
2216	25	-3.4102909824375125
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	128845.0
3	6.0
4	929.0
5	5395.0
6	6626.0
7	6103.0
8	5438.0
9	5019.0
10	4655.0
11	4591.0
12	4422.0
13	4468.0
14	4319.0
15	4476.0
16	4717.0
17	4961.0
18	5831.0
19	7058.0
20	8696.0
21	10882.0
22	13523.0
23	16021.0
24	19120.0
25	21976.0
26	25261.0
27	28261.0
28	32569.0
29	39221.0
30	49186.0
31	63484.0
32	81567.0
33	107985.0
34	147836.0
35	204565.0
36	303600.0
37	534915.0
38	1514678.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.26796918982248	18.67817269102158	12.637070996602265	25.416787122553675
2	15.610394329085272	20.68658264463659	39.42272445389741	24.280298572380733
3	17.686518511908552	25.850920216405477	29.45118122740334	27.011380044282635
4	12.038051455478445	17.195077558976525	38.522915419029836	32.24395556651519
5	14.121395357849783	37.06744440993114	33.99377703610059	14.817383196118486
6	30.682657937038044	37.814549217929994	18.541613757973003	12.96117908705896
7	27.99355630219134	31.75415166886018	22.373086109579273	17.87920591936921
8	26.354020839401315	33.22650752116355	20.92804275929458	19.491428880140557
9	27.31697220839957	15.233754471566197	19.895750305688523	37.55352301434571
10	15.374720201968312	27.47582178057963	32.3422012490918	24.807256768360254
11	34.980637395223205	22.787774695027966	23.46222163503178	18.769366274717047
12	23.98509879039243	25.007149362664414	29.61136827937889	21.396383567564268
13	28.393144611377163	21.053428942122977	25.732722287353095	24.820704159146768
14	23.046773949645218	21.34703219810696	26.51825577315244	29.087938079095384
15	24.211505152592917	27.83043202385605	23.37392435934289	24.584138464208145
16	24.421099608331325	27.324673951978014	24.561706900297715	23.692519539392944
17	23.13648515527394	26.97243342518766	25.88536168925833	24.00571973028007
18	23.51311695248252	26.392906336593718	27.037397768577982	23.05657894234578
19	25.0122080743308	26.067637576849208	26.410726309215033	22.509428039604963
20	25.07473230405691	25.590730933463075	25.78645455151369	23.548082210966335
21	26.530881288600693	25.321115193937516	25.015465968263566	23.132537549198222
22	25.06593640858433	25.485603810716263	25.601620176922758	23.846839603776655
23	23.74874409039848	25.228676842348186	26.016251807893248	25.006327259360084
24	23.76627670307136	26.41858506753797	25.936963780481932	23.87817444890874
25	23.968036080529345	25.724700980557678	25.989138844559807	24.31812409435317
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	15932.0
1	15932.0
2	12706.5
3	9481.0
4	9481.0
5	9481.0
6	6609.5
7	3738.0
8	3738.0
9	3738.0
10	5658.5
11	7579.0
12	7579.0
13	7579.0
14	12766.5
15	17954.0
16	17954.0
17	17954.0
18	29398.0
19	40842.0
20	40842.0
21	40842.0
22	61577.0
23	82312.0
24	82312.0
25	82312.0
26	112874.0
27	143436.0
28	143436.0
29	143436.0
30	169705.0
31	195974.0
32	195974.0
33	195974.0
34	235757.5
35	275541.0
36	275541.0
37	275541.0
38	299988.0
39	324435.0
40	324435.0
41	324435.0
42	353116.0
43	381797.0
44	381797.0
45	381797.0
46	407224.5
47	432652.0
48	432652.0
49	432652.0
50	426316.0
51	419980.0
52	419980.0
53	419980.0
54	377412.0
55	334844.0
56	334844.0
57	334844.0
58	296933.5
59	259023.0
60	259023.0
61	259023.0
62	228122.5
63	197222.0
64	197222.0
65	197222.0
66	162956.5
67	128691.0
68	128691.0
69	128691.0
70	98409.5
71	68128.0
72	68128.0
73	68128.0
74	54594.0
75	41060.0
76	41060.0
77	41060.0
78	34380.0
79	27700.0
80	27700.0
81	27700.0
82	20370.5
83	13041.0
84	13041.0
85	13041.0
86	9915.5
87	6790.0
88	6790.0
89	6790.0
90	4755.5
91	2721.0
92	2721.0
93	2721.0
94	1480.5
95	240.0
96	240.0
97	240.0
98	166.0
99	92.0
100	92.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.39732397219052784
2	0.6972477598977619
3	0.7126359398520344
4	0.7217580995597757
5	0.7318420205146589
6	0.7338529758495922
7	0.7492120115236484
8	0.7387783592061681
9	0.7274412342019786
10	0.7207380497522007
11	0.7331243688441816
12	0.7375542994370783
13	0.7356599212230106
14	0.7452192451339981
15	0.7415470658267285
16	0.7363302396679884
17	0.7417219315080271
18	0.7453358222548638
19	0.7481919617160735
20	0.7397401204533101
21	0.7385160606842203
22	0.7475799318315286
23	0.7375542994370783
24	0.740760170260885
25	0.7428877027166841
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	3431205.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	65.37855088669295
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.81538845027949	60.02757045978079
2	5.615182800885124	7.342250289715024
3	1.0260056140422495	2.012362807430816
4	0.42430870533019127	1.1096275313238686
5	0.23551911089993005	0.769894908837988
6	0.1555065070224751	0.6100074049548458
7	0.11123828804526284	0.5090818652861078
8	0.07252569603537494	0.37932999270732687
9	0.0630414124814958	0.37094005745014275
>10	0.353845920561457	4.506808692069837
>50	0.051867385426621095	2.403296697250189
>100	0.05978833962360745	8.452386355720282
>500	0.010132613288374172	4.5418657606669415
>1k	0.005559486934680678	6.477965452427988
>5k	4.483457205387644E-5	0.1534200208055606
>10k+	4.483457205387644E-5	0.3331917035721833
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
NNNNNNNNNNNNNNNNNNNNNNNNN	11367	0.33128303322010777	No Hit
GNNNNNNNNNNNNNNNNNNNNNNNN	5234	0.15254116265277068	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG	4112	0.11984128024994135	No Hit
GTCCTATTCCATTATTCCTAGCTGC	4100	0.11949154888734423	No Hit
CTATTGGAGCTGGAATTACCGCGGC	3901	0.11369183712427557	No Hit
GTATCTGATCGTCTTCGAACCTCCG	3839	0.11188489175085721	No Hit
GAATAGGACCGCGGTTCTATTTTGT	3837	0.11182660319042435	No Hit
CTCTTAATCATGGCCTCAGTTCCGA	3501	0.10203412503770541	No Hit
GATTAAGAGGGACGGCCGGGGGCAT	3459	0.10081006526861554	No Hit
CTTTAATATACGCTATTGGAGCTGG	3448	0.10048947818623487	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	2.9144280216425426E-5	0.0
6	0.0	0.0	0.0	2.9144280216425426E-5	0.0
7	0.0	0.0	0.0	2.9144280216425426E-5	0.0
8	0.0	0.0	0.0	2.9144280216425426E-5	0.0
9	0.0	0.0	0.0	8.743284064927628E-5	2.9144280216425426E-5
10	0.0	0.0	0.0	1.4572140108212711E-4	2.9144280216425426E-5
11	2.9144280216425426E-5	0.0	0.0	1.4572140108212711E-4	2.9144280216425426E-5
12	2.9144280216425426E-5	0.0	0.0	1.4572140108212711E-4	5.828856043285085E-5
13	2.9144280216425426E-5	0.0	0.0	1.4572140108212711E-4	8.743284064927628E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1410	0.0	16.045776	1
GTATCAA	4805	0.0	13.215571	1
CTCGACG	130	1.4551915E-11	13.153226	13
CGAACGA	185	0.0	12.837233	16
TCGACGC	140	5.2750693E-11	12.21371	14
CGCATCG	530	0.0	12.188105	13
GTGATCG	55	0.0030708201	12.090339	9
CCGCATC	555	0.0	11.810254	12
ATCGCCA	550	0.0	11.744901	16
CGACCAT	455	0.0	11.691757	10
TCGCCAG	545	0.0	11.678349	17
CGTCGTA	400	0.0	11.636951	10
CGCCAGT	565	0.0	11.433258	18
AGGCCCG	765	0.0	11.424298	10
ACGAACG	210	0.0	11.308825	15
CCGTCGT	440	0.0	11.226744	9
CATCGCC	570	0.0	11.165977	15
AACCGCG	350	0.0	11.12821	7
CGAGCCG	770	0.0	11.1032095	15
GCATCGC	610	0.0	11.056855	14
>>END_MODULE