FastQCFastQC Report
Thu 2 Feb 2017
SRR4050218_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4050218_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3431205
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCAAAGCAGGCCCGAGCCGCCTG41720.12158993706292687No Hit
CTATTGGAGCTGGAATTACCGCGGC40310.11748059355241089No Hit
GAATAGGACCGCGGTTCTATTTTGT38370.11182660319042435No Hit
GATTAAGAGGGACGGCCGGGGGCAT36410.10611432426800498No Hit
CTCTTAATCATGGCCTCAGTTCCGA36120.10526914014172863No Hit
GTCCTATTCCATTATTCCTAGCTGC35750.10419080177372089No Hit
GTATCAACGCAGAGTACTTTTTTTT35640.10387021469134021No Hit
GTATCTGATCGTCTTCGAACCTCCG35370.10308331912549673No Hit
CTTTAATATACGCTATTGGAGCTGG35060.10217984643878754No Hit
ATACAGGACTCTTTCGAGGCCCTGT34740.10124722947186192No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA14750.016.0406841
ATCGCCA5400.014.2416
CGCCAGT5600.013.56190418
CGCATCG5400.013.53757913
GTATCAA50250.013.3279791
GCATCGC5750.013.04377414
CGTCGTA4700.012.92784510
CATCGCC5850.012.82005615
CGCGCCA1501.8189894E-1212.65851510
TCGCCAG5950.012.60459317
GCCGCGC2050.012.5041438
CCGCATC5950.012.44576612
CCGTCGT5200.012.04993259
GCGTTAT1750.011.9935531
GTCGTAG5200.011.86735811
CGTTATT1701.8189894E-1211.7498322
GCGTAAC2550.011.54158711
ACCGTCG5850.011.3602078
GCTCGTA3100.011.3314129
CGCGTAA2600.011.319633510
Produced by FastQC (version 0.11.5)