##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4050218_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3431205
Sequences flagged as poor quality	0
Sequence length	25
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.885100423903555	31.0	31.0	34.0	27.0	34.0
2	30.925584160666588	31.0	31.0	34.0	27.0	34.0
3	31.117329917623692	31.0	31.0	34.0	27.0	34.0
4	34.72764436983509	37.0	35.0	37.0	32.0	37.0
5	34.62691649143668	37.0	35.0	37.0	32.0	37.0
6	34.6593855511402	37.0	35.0	37.0	32.0	37.0
7	34.58849092374253	37.0	35.0	37.0	32.0	37.0
8	34.57352650162261	37.0	35.0	37.0	32.0	37.0
9	36.202038933843944	39.0	37.0	39.0	32.0	39.0
10	36.09094705795777	39.0	37.0	39.0	32.0	39.0
11	36.078067034758924	39.0	37.0	39.0	32.0	39.0
12	35.990137867017566	39.0	37.0	39.0	31.0	39.0
13	35.99090290437324	39.0	37.0	39.0	31.0	39.0
14	37.174980509762605	40.0	37.0	41.0	31.0	41.0
15	37.150125684708435	40.0	37.0	41.0	31.0	41.0
16	37.11361023313967	40.0	37.0	41.0	31.0	41.0
17	37.105448960350664	40.0	37.0	41.0	31.0	41.0
18	37.07578707771759	40.0	37.0	41.0	31.0	41.0
19	37.118082714381686	40.0	37.0	41.0	31.0	41.0
20	37.09154947022984	40.0	37.0	41.0	31.0	41.0
21	37.015943961378	40.0	37.0	41.0	31.0	41.0
22	36.94779705671914	40.0	37.0	41.0	31.0	41.0
23	36.85701262384497	40.0	37.0	41.0	31.0	41.0
24	36.78897850755055	40.0	37.0	41.0	31.0	41.0
25	36.51361985075214	40.0	37.0	41.0	30.0	41.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.8353769536783666
1101	2	0.8958770367146265
1101	3	0.8570924045270516
1101	4	0.7102093204813755
1101	5	0.8824957166502188
1101	6	0.7497418690694175
1101	7	0.8655487569750591
1101	8	0.8579411205771024
1101	9	0.9803653454783898
1101	10	1.0254801614388427
1101	11	1.0599715846035593
1101	12	1.0632288456442893
1101	13	1.097858854721018
1101	14	1.2531631523668025
1101	15	1.2558123552486578
1101	16	1.2576814756051036
1101	17	1.202261364107514
1101	18	1.2021728658785094
1101	19	1.2230991231608215
1101	20	1.3052359630572212
1101	21	1.2047559125195804
1101	22	1.235679776336994
1101	23	1.2595478187095566
1101	24	1.2714700125281553
1101	25	1.3065227934221753
1102	1	0.755188426566999
1102	2	0.847250470011975
1102	3	0.8265199455372958
1102	4	0.6864283204879413
1102	5	0.8242301569413897
1102	6	0.7701089678641964
1102	7	0.8189915759138486
1102	8	0.8242017908616859
1102	9	0.9065920946211108
1102	10	1.033504061316009
1102	11	0.9752706722414857
1102	12	1.0764736368119685
1102	13	1.0053569074473003
1102	14	1.2182723933044315
1102	15	1.2591350173856597
1102	16	1.1709826275862554
1102	17	1.229389429594896
1102	18	1.2544119380980803
1102	19	1.1394727196229155
1102	20	1.1756472400149605
1102	21	1.1740480220342349
1102	22	1.2475470384392224
1102	23	1.2328522587219624
1102	24	1.2600459591524427
1102	25	1.1898760791558018
1103	1	0.6489194447923055
1103	2	0.6303294431531086
1103	3	0.6382018283899917
1103	4	0.5919432751153764
1103	5	0.5729505286776444
1103	6	0.5933836290030712
1103	7	0.6661147163978285
1103	8	0.692718759503876
1103	9	0.804623107208954
1103	10	0.7900521882334175
1103	11	0.8081984336573385
1103	12	0.8158140042210391
1103	13	0.8612984772205508
1103	14	0.9817018504398121
1103	15	0.9625272980001611
1103	16	0.9566248387730383
1103	17	0.9523531794104088
1103	18	1.0095798355515484
1103	19	0.9934689105768868
1103	20	1.0397746301361437
1103	21	1.034896948666706
1103	22	1.0816607493308794
1103	23	1.117544506823755
1103	24	1.078484795025311
1103	25	1.0816165645263922
1104	1	0.6431342996747453
1104	2	0.6283774289653188
1104	3	0.6533621231919469
1104	4	0.5700703755602845
1104	5	0.5560233311896923
1104	6	0.6253065873425214
1104	7	0.6630872225412645
1104	8	0.6745150621502063
1104	9	0.7319022136797955
1104	10	0.7664537140156185
1104	11	0.7773304638956162
1104	12	0.7880856673246726
1104	13	0.7896279898732388
1104	14	0.9426485976390637
1104	15	0.9723739156991726
1104	16	0.984191840750043
1104	17	0.9373660645888862
1104	18	0.8846393418528606
1104	19	0.9204437632528624
1104	20	0.9138943129790107
1104	21	0.9066945672387945
1104	22	1.0370831156766513
1104	23	1.0389285771360335
1104	24	1.001813616860197
1104	25	1.001581561938636
1105	1	0.4586431310573822
1105	2	0.5002465640889397
1105	3	0.4906179347166386
1105	4	0.4678471400700914
1105	5	0.4906060480924168
1105	6	0.42638843027415874
1105	7	0.534750528456513
1105	8	0.5222879308130501
1105	9	0.6132212074410006
1105	10	0.7096745861351437
1105	11	0.7099975442523601
1105	12	0.6577968491632049
1105	13	0.6944039358433614
1105	14	0.7562377566088898
1105	15	0.8192963695655067
1105	16	0.8041718360288996
1105	17	0.777004520107127
1105	18	0.7315875758481383
1105	19	0.7585698394496987
1105	20	0.7354609804748193
1105	21	0.7193130590534764
1105	22	0.7784440956312437
1105	23	0.8479284613313709
1105	24	0.8547103067410617
1105	25	0.8343492424534844
1106	1	0.4511167591986194
1106	2	0.5207395689239327
1106	3	0.5002390079459076
1106	4	0.4115064986348713
1106	5	0.350669601488903
1106	6	0.47350414808948926
1106	7	0.5151802121165971
1106	8	0.5783864655655151
1106	9	0.598160814533081
1106	10	0.5111839008484864
1106	11	0.5943583390683571
1106	12	0.5867035084071546
1106	13	0.6619332402840428
1106	14	0.7190171099455469
1106	15	0.7202393146858199
1106	16	0.7578513594286349
1106	17	0.7447096774737219
1106	18	0.6972964122007284
1106	19	0.7520295070731535
1106	20	0.8318348170427612
1106	21	0.7658008764036808
1106	22	0.8493824912484698
1106	23	0.8586440748666533
1106	24	0.8191471023437913
1106	25	0.8030099994471769
1107	1	0.3824276375546347
1107	2	0.43298541107078137
1107	3	0.3716854181409275
1107	4	0.3394638540371844
1107	5	0.27645368996228115
1107	6	0.2727142506637037
1107	7	0.4015512106435182
1107	8	0.3710992112459195
1107	9	0.44042048069180595
1107	10	0.42522517937123183
1107	11	0.4979990124917393
1107	12	0.49680055422410874
1107	13	0.4777876663417189
1107	14	0.5199987037389349
1107	15	0.5614783842190647
1107	16	0.5635912461935675
1107	17	0.4989644747338744
1107	18	0.4437481659563929
1107	19	0.5418529580814209
1107	20	0.5817400028543673
1107	21	0.577797842800031
1107	22	0.5928579520979724
1107	23	0.6174796406604912
1107	24	0.5779108326674276
1107	25	0.5773993332084757
1108	1	0.31517376734726454
1108	2	0.2537277297073217
1108	3	0.26910323780317924
1108	4	0.2430586093916247
1108	5	0.13841314412156436
1108	6	0.1424013260327328
1108	7	0.3265995098752654
1108	8	0.2936653463036407
1108	9	0.36733670843682376
1108	10	0.3172016913270781
1108	11	0.29238779208326804
1108	12	0.34813589538624967
1108	13	0.3693584771788494
1108	14	0.4246983449133097
1108	15	0.3288496039959057
1108	16	0.46404727340474494
1108	17	0.5127476758637357
1108	18	0.4520527388246549
1108	19	0.5045115676995664
1108	20	0.42493555181707166
1108	21	0.4644862038822879
1108	22	0.5278537327917121
1108	23	0.48821980632866513
1108	24	0.39765646219647977
1108	25	0.3709764181390156
1109	1	0.2957147952262851
1109	2	0.2873079717587075
1109	3	0.24185442926784262
1109	4	0.1878010134635062
1109	5	0.19415333264505108
1109	6	0.24345237629673733
1109	7	0.3441670703594255
1109	8	0.31612662095726307
1109	9	0.37302341014558493
1109	10	0.4126914162060942
1109	11	0.3895058508214362
1109	12	0.40913239774099
1109	13	0.3440479016080218
1109	14	0.3988826515092825
1109	15	0.4764880878122284
1109	16	0.5045335550390675
1109	17	0.42821258331404977
1109	18	0.5421398774946908
1109	19	0.5402732161163044
1109	20	0.5492072889194972
1109	21	0.5408877730555233
1109	22	0.5295196087139189
1109	23	0.5253975696972191
1109	24	0.5899568967352309
1109	25	0.5311000462776576
1110	1	-0.06339816003659848
1110	2	-0.01366011867404282
1110	3	-0.04189667182374279
1110	4	-0.06447439732063032
1110	5	-0.18801728367171933
1110	6	-0.17979772477610823
1110	7	-0.09799750836092613
1110	8	-0.10152304048217786
1110	9	0.010255567107023467
1110	10	-0.07827267147426653
1110	11	-0.06763437670625905
1110	12	-0.11280335473630032
1110	13	-0.029481187290571143
1110	14	-0.0976614604285615
1110	15	-0.08224868904837734
1110	16	-0.13998836371175116
1110	17	-0.16186447301632256
1110	18	-0.17379669962991073
1110	19	-0.16908251478093206
1110	20	-0.204545994274568
1110	21	-0.24061556167900022
1110	22	-0.23003679528829224
1110	23	-0.1370638418955039
1110	24	-0.2180670153595088
1110	25	-0.2464631209035204
1111	1	-0.14988338424287662
1111	2	-0.19069536591608127
1111	3	-0.2145894254322549
1111	4	-0.19375284648248225
1111	5	-0.268627756588252
1111	6	-0.2724022337094212
1111	7	-0.3359980298297458
1111	8	-0.25229119443191905
1111	9	-0.3206163996404783
1111	10	-0.3086786071057759
1111	11	-0.3487945435762754
1111	12	-0.3627309566378898
1111	13	-0.32895870590903087
1111	14	-0.3657154197944905
1111	15	-0.4217574198540248
1111	16	-0.3574597208976371
1111	17	-0.38413448248376625
1111	18	-0.3735906435036682
1111	19	-0.38654412606895505
1111	20	-0.37563092787588914
1111	21	-0.32263513954239187
1111	22	-0.3641390841820211
1111	23	-0.32907271192825505
1111	24	-0.3854163589669568
1111	25	-0.44944511250939456
1112	1	-0.37536872095050455
1112	2	-0.48735467950889344
1112	3	-0.35869839528014325
1112	4	-0.3154549590251179
1112	5	-0.40556832525603426
1112	6	-0.3760602751753481
1112	7	-0.43010750717505886
1112	8	-0.4673051675277904
1112	9	-0.5096101337388887
1112	10	-0.48610901229172043
1112	11	-0.46789996094216235
1112	12	-0.42134775547943804
1112	13	-0.3692387768233374
1112	14	-0.5191090551000741
1112	15	-0.5699838855065948
1112	16	-0.5348842052802496
1112	17	-0.6108589587464195
1112	18	-0.5186959999698644
1112	19	-0.5269754312170107
1112	20	-0.5285805029313764
1112	21	-0.5058924610386342
1112	22	-0.48330286375220766
1112	23	-0.44863318532186014
1112	24	-0.39106142203197436
1112	25	-0.467425746407514
1113	1	-0.30994046178328816
1113	2	-0.31002501565096097
1113	3	-0.36228212015361905
1113	4	-0.40690567188704563
1113	5	-0.4787493219683938
1113	6	-0.48180038312393947
1113	7	-0.42852253810996643
1113	8	-0.45162596672270894
1113	9	-0.5034607692635547
1113	10	-0.46403047613411985
1113	11	-0.5177394213933724
1113	12	-0.4272793055253885
1113	13	-0.5507479699270519
1113	14	-0.626589453823847
1113	15	-0.5938929122866483
1113	16	-0.5513588231241258
1113	17	-0.5314203981903631
1113	18	-0.5580300095998041
1113	19	-0.6545843505662106
1113	20	-0.5763864219828818
1113	21	-0.6802807200116945
1113	22	-0.657823793352641
1113	23	-0.5710115247386227
1113	24	-0.6359669508376768
1113	25	-0.6735121732315719
1114	1	-0.5366608898031586
1114	2	-1.1231029366281184
1114	3	-0.7105564834285545
1114	4	-0.5798857902906249
1114	5	-0.7177954462533194
1114	6	-0.6786805044084829
1114	7	-0.6408022224060659
1114	8	-0.6742445654954139
1114	9	-0.725677869766308
1114	10	-0.7351515356286029
1114	11	-0.7441978901634769
1114	12	-0.7867800046818516
1114	13	-0.74721865984354
1114	14	-0.9505831925217905
1114	15	-0.9945186249606905
1114	16	-0.9365104016645134
1114	17	-0.9303356514085905
1114	18	-0.9013240573963088
1114	19	-0.9024200135164335
1114	20	-0.906825510611867
1114	21	-0.9642656738473292
1114	22	-1.014540309102557
1114	23	-1.0928451225781473
1114	24	-0.98314830020756
1114	25	-1.0764540165244938
1115	1	-0.8285815585920453
1115	2	-1.1511780091864843
1115	3	-1.097229114395791
1115	4	-0.8925488313622978
1115	5	-1.093147878468848
1115	6	-1.0476727836186583
1115	7	-1.1155165400621954
1115	8	-1.1148688872680665
1115	9	-1.5447045626081035
1115	10	-1.3122884198801685
1115	11	-1.3781479354560844
1115	12	-1.3055755877100026
1115	13	-1.687420533104067
1115	14	-1.6852203624147037
1115	15	-1.636208019931324
1115	16	-1.7013340393193133
1115	17	-1.6166705422389356
1115	18	-1.6654781462769606
1115	19	-1.5873146706292829
1115	20	-1.6275410681427687
1115	21	-1.6341059115009244
1115	22	-1.8055181154535802
1115	23	-1.811932961801162
1115	24	-1.7681879100515516
1115	25	-1.6591616996479317
1116	1	-0.6789448131998661
1116	2	-0.6432092529193412
1116	3	-0.7434120635767698
1116	4	-0.7767376644657276
1116	5	-0.8902506483070312
1116	6	-0.9409857074956278
1116	7	-1.038653225289785
1116	8	-1.0187389526817938
1116	9	-1.1211750636483444
1116	10	-1.0652840060663777
1116	11	-1.1782445475793324
1116	12	-1.3951222954862317
1116	13	-1.272051619537038
1116	14	-1.42770371251855
1116	15	-1.4603770074397602
1116	16	-1.5507237611147673
1116	17	-1.6855097318829522
1116	18	-1.52252751786218
1116	19	-1.4944916085830258
1116	20	-1.4529482254618387
1116	21	-1.4793429687026247
1116	22	-1.5156839255741303
1116	23	-1.7474426256293398
1116	24	-1.6911318942029752
1116	25	-1.7841470944758697
1201	1	0.6825220285671527
1201	2	0.7564451275542723
1201	3	0.728372739691757
1201	4	0.6558711147103082
1201	5	0.8903697776715731
1201	6	0.8169499588654219
1201	7	0.8420684323434386
1201	8	0.8325713633840479
1201	9	0.970515799488183
1201	10	1.0148512154576252
1201	11	0.9766272913856611
1201	12	0.9964656095906008
1201	13	0.9494797660841385
1201	14	1.2217571159426228
1201	15	1.1770706820632384
1201	16	1.20137638527423
1201	17	1.2093181446845023
1201	18	1.1920985013303635
1201	19	1.242724048692395
1201	20	1.189638396489812
1201	21	1.1943183014812888
1201	22	1.2749944686863515
1201	23	1.2457939285947575
1201	24	1.2877097590769964
1201	25	1.2787873439372532
1202	1	0.6912173758305258
1202	2	0.6985864827586354
1202	3	0.7155042241797105
1202	4	0.7491198719584773
1202	5	0.855116183830809
1202	6	0.8760571411703637
1202	7	0.8842337484342835
1202	8	0.8129269429394483
1202	9	0.9313845160379088
1202	10	0.9249539433753355
1202	11	0.9744556168937208
1202	12	0.9553788840385948
1202	13	0.9739694284226488
1202	14	1.1019456705868862
1202	15	1.1449338735831844
1202	16	1.1251482729596205
1202	17	1.0844335368073317
1202	18	1.1572750346225575
1202	19	1.069033102050362
1202	20	1.0578158847109904
1202	21	1.1287952119528981
1202	22	1.107548313473572
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2211	2	-0.4951221032435349
2211	3	-0.4548293033947033
2211	4	-0.3747713100804546
2211	5	-0.34297889708444984
2211	6	-0.3478341727490317
2211	7	-0.41922941367864297
2211	8	-0.37794873768149273
2211	9	-0.38437163849358313
2211	10	-0.48641731764869434
2211	11	-0.5201570096809078
2211	12	-0.531894406380836
2211	13	-0.44156066415555273
2211	14	-0.6675909975511871
2211	15	-0.6126412742595235
2211	16	-0.595132172728519
2211	17	-0.5402154115307596
2211	18	-0.6553316825769357
2211	19	-0.6002891472952712
2211	20	-0.6547521538327743
2211	21	-0.6961334360067113
2211	22	-0.6102720340615946
2211	23	-0.6667501859824725
2211	24	-0.6839428188474983
2211	25	-0.6327231127342756
2212	1	-0.4602491243239122
2212	2	-0.5040646022744468
2212	3	-0.5470484867919474
2212	4	-0.48772866191172426
2212	5	-0.55512735511698
2212	6	-0.5323109871447897
2212	7	-0.7062933872072605
2212	8	-0.5933084511308522
2212	9	-0.6434971736167725
2212	10	-0.7665975609752422
2212	11	-0.7587334255943077
2212	12	-0.8489289532208844
2212	13	-0.8173354926507059
2212	14	-0.9043987934370676
2212	15	-0.8937538871014112
2212	16	-0.8363239973472076
2212	17	-0.8440590905082317
2212	18	-0.8966651498593663
2212	19	-0.9142056422637381
2212	20	-0.8745164864364696
2212	21	-0.9105241545006635
2212	22	-0.8642440114089709
2212	23	-0.87215853048464
2212	24	-0.8723193011604451
2212	25	-0.8234785783371592
2213	1	-0.754650690360787
2213	2	-0.7230832169758408
2213	3	-0.6652111430180554
2213	4	-0.6662228101033989
2213	5	-0.6741854352431744
2213	6	-0.7020888271972794
2213	7	-0.7506078195007007
2213	8	-0.7830258362078979
2213	9	-0.9259561359706581
2213	10	-1.0204083567111013
2213	11	-0.8850696083477416
2213	12	-0.9370569014659011
2213	13	-1.104182709821906
2213	14	-1.200182500142894
2213	15	-1.139615826413376
2213	16	-1.2217595539933512
2213	17	-1.1809965336439276
2213	18	-1.195091003972479
2213	19	-1.179855801398709
2213	20	-1.131743482336077
2213	21	-1.186127099468635
2213	22	-1.216790540534582
2213	23	-1.1573853534076335
2213	24	-1.1346094716644544
2213	25	-1.1778217935171398
2214	1	-0.8070623723975956
2214	2	-1.2800432812571252
2214	3	-0.9036612051512094
2214	4	-0.7378723150720603
2214	5	-0.8194498746020358
2214	6	-0.8986585993791749
2214	7	-0.8023138717839444
2214	8	-0.937540403961421
2214	9	-1.1714528887582532
2214	10	-1.0736944380365543
2214	11	-1.0905198603682535
2214	12	-1.1417290826554947
2214	13	-1.102172881030377
2214	14	-1.370566683650594
2214	15	-1.3748843664391899
2214	16	-1.4531767732287832
2214	17	-1.2491283622107758
2214	18	-1.2036145578815365
2214	19	-1.2432235689101034
2214	20	-1.3901389877696815
2214	21	-1.3672843184167291
2214	22	-1.4029284513823796
2214	23	-1.3739427179995118
2214	24	-1.3737969268431627
2214	25	-1.3748629087185265
2215	1	-0.9296005358048198
2215	2	-1.1188461349435705
2215	3	-1.0334595395885593
2215	4	-0.8531090023929551
2215	5	-1.0904012694295844
2215	6	-1.0643785643565664
2215	7	-0.9910104116950862
2215	8	-1.0643300135130573
2215	9	-1.3680471933859337
2215	10	-1.2876907498179975
2215	11	-1.3562907852462587
2215	12	-1.5124751930535396
2215	13	-1.6072466887463293
2215	14	-1.6341116399547744
2215	15	-1.882576880722155
2215	16	-1.6845937899260548
2215	17	-1.6057319103321035
2215	18	-1.692321789512981
2215	19	-1.6610336462219522
2215	20	-1.618991436420103
2215	21	-1.6154267382504983
2215	22	-1.54530833993352
2215	23	-1.5629815969360905
2215	24	-1.6060343503459436
2215	25	-1.54196650808872
2216	1	-0.9030513992772669
2216	2	-0.8939638146485613
2216	3	-0.9341726983307801
2216	4	-0.9093915170599587
2216	5	-0.9369839118958581
2216	6	-0.9132390633083318
2216	7	-0.9855851481727385
2216	8	-1.04847189361341
2216	9	-1.2742088460952417
2216	10	-1.2258203585154703
2216	11	-1.3182336682653784
2216	12	-1.4099956772141908
2216	13	-1.2572314298446159
2216	14	-1.3391434656640655
2216	15	-1.4810354611574112
2216	16	-1.3794896634353293
2216	17	-1.4960117353591187
2216	18	-1.6738502445802155
2216	19	-1.538523679406481
2216	20	-1.6527643661419589
2216	21	-1.5312678490094385
2216	22	-1.643980449525209
2216	23	-1.5528474745313758
2216	24	-1.6503491286002117
2216	25	-1.5441906640598333
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	42318.0
3	5.0
4	794.0
5	6889.0
6	7973.0
7	6309.0
8	5213.0
9	4537.0
10	4199.0
11	3926.0
12	3644.0
13	3552.0
14	3508.0
15	3645.0
16	3710.0
17	3922.0
18	4314.0
19	5186.0
20	6584.0
21	8916.0
22	11463.0
23	14255.0
24	17513.0
25	20546.0
26	22816.0
27	25887.0
28	30196.0
29	37424.0
30	47937.0
31	63198.0
32	82532.0
33	109782.0
34	148340.0
35	206287.0
36	299952.0
37	524185.0
38	1639748.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.2869382237099	19.335514501862754	13.152118354544884	26.225428919882454
2	15.620347625970405	21.299551973102066	37.90461445554181	25.175485945385716
3	17.36943726766544	25.937156188569322	29.59444276864833	27.098963775116907
4	12.320874230808803	17.892335237226973	37.55092845053641	32.23586208142781
5	13.972405268822824	37.337779582537955	33.93729223890523	14.752522909733997
6	30.682060377299074	38.23407727391605	18.16316349733614	12.920698851448728
7	27.907014699959138	31.882864309100757	22.23585218552527	17.974268805414837
8	26.37090761991307	33.59611843573986	20.645932582069097	19.38704136227797
9	27.1658124938979	15.148570687471857	19.80857980965815	37.877037008972096
10	15.255579438230901	27.756260701664903	32.28911800065576	24.699041859448432
11	34.96179704285562	22.691763806166332	23.477215206105946	18.869223944872097
12	23.860377748924183	25.245175402165188	29.479105188838027	21.415341660072603
13	28.482279946418394	20.961997395532766	25.64610373334173	24.909618924707114
14	22.972337637921964	21.3800721981675	26.466705627055802	29.18088453685473
15	24.20881870156033	27.955840040663677	23.26007731694351	24.575263940832485
16	24.46977424676059	27.383349168507625	24.381058411391997	23.765818173339785
17	23.119795182450606	27.132146258943575	25.832712515112405	23.915346043493415
18	23.533371978851633	26.485061875088707	27.058099718843653	22.923466427216006
19	25.074019962292738	26.064207271632494	26.331055101045735	22.53071766502903
20	25.203020053540225	25.55378577788529	25.690944356927076	23.552249811647403
21	26.457903985138177	25.33536884817082	25.066356792758416	23.140370373932587
22	25.01969452286794	25.553566916349208	25.553421192909564	23.873317367873288
23	23.694788597342566	25.205253079889133	26.060044358564088	25.039913964204214
24	23.766003217037536	26.412670896011115	25.907207207469774	23.91411867948158
25	23.91646689352152	25.750231348231917	26.017939506991457	24.31536225125511
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	273.0
1	273.0
2	539.0
3	805.0
4	805.0
5	805.0
6	2159.5
7	3514.0
8	3514.0
9	3514.0
10	5479.0
11	7444.0
12	7444.0
13	7444.0
14	12624.5
15	17805.0
16	17805.0
17	17805.0
18	29285.0
19	40765.0
20	40765.0
21	40765.0
22	61993.5
23	83222.0
24	83222.0
25	83222.0
26	114575.5
27	145929.0
28	145929.0
29	145929.0
30	172742.0
31	199555.0
32	199555.0
33	199555.0
34	238479.0
35	277403.0
36	277403.0
37	277403.0
38	302718.0
39	328033.0
40	328033.0
41	328033.0
42	356136.5
43	384240.0
44	384240.0
45	384240.0
46	409278.0
47	434316.0
48	434316.0
49	434316.0
50	428100.5
51	421885.0
52	421885.0
53	421885.0
54	379283.0
55	336681.0
56	336681.0
57	336681.0
58	298985.5
59	261290.0
60	261290.0
61	261290.0
62	229872.0
63	198454.0
64	198454.0
65	198454.0
66	163781.5
67	129109.0
68	129109.0
69	129109.0
70	98550.5
71	67992.0
72	67992.0
73	67992.0
74	54567.5
75	41143.0
76	41143.0
77	41143.0
78	34885.0
79	28627.0
80	28627.0
81	28627.0
82	20837.0
83	13047.0
84	13047.0
85	13047.0
86	9846.0
87	6645.0
88	6645.0
89	6645.0
90	4692.0
91	2739.0
92	2739.0
93	2739.0
94	1484.0
95	229.0
96	229.0
97	229.0
98	144.5
99	60.0
100	60.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.4458783430310926
2	0.004692229114844493
3	0.0
4	0.004837950515926621
5	0.16600582011275922
6	0.04097685798429415
7	0.0011366269284405915
8	3.2058708238067964E-4
9	2.9144280216425423E-4
10	0.002331542417314034
11	0.0016612239723362491
12	0.0026229852194782884
13	0.0051585375983073
14	0.010783383680077406
15	0.0038761892687845813
16	7.286070054106356E-4
17	0.0072569257738899304
18	5.245970438956576E-4
19	0.0010491940877913153
20	0.0040801992302995594
21	0.003847044988568156
22	0.0013989254503884204
23	0.002185821016231907
24	0.0028561394612096917
25	0.006703184449777847
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	3431205.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	65.15699379686151
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.66424660101455	59.7256674717629
2	5.73993961994933	7.479944204227962
3	1.0506271308204178	2.0536711633704114
4	0.4162593566707534	1.0848883328192738
5	0.23714479817399298	0.7725821071790419
6	0.14934066819916159	0.5838353394883155
7	0.1040723852007672	0.474673062986568
8	0.0827169876807209	0.43116722025662413
9	0.057958432217384205	0.3398757487617529
>10	0.3683703516116766	4.644082666923152
>50	0.05146020849254816	2.3952477538605605
>100	0.061241475275136584	8.557831310086325
>500	0.011111191926515475	4.994822008774492
>1k	0.0055107927671484355	6.46171160950236
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTTCAAAGCAGGCCCGAGCCGCCTG	4172	0.12158993706292687	No Hit
CTATTGGAGCTGGAATTACCGCGGC	4031	0.11748059355241089	No Hit
GAATAGGACCGCGGTTCTATTTTGT	3837	0.11182660319042435	No Hit
GATTAAGAGGGACGGCCGGGGGCAT	3641	0.10611432426800498	No Hit
CTCTTAATCATGGCCTCAGTTCCGA	3612	0.10526914014172863	No Hit
GTCCTATTCCATTATTCCTAGCTGC	3575	0.10419080177372089	No Hit
GTATCAACGCAGAGTACTTTTTTTT	3564	0.10387021469134021	No Hit
GTATCTGATCGTCTTCGAACCTCCG	3537	0.10308331912549673	No Hit
CTTTAATATACGCTATTGGAGCTGG	3506	0.10217984643878754	No Hit
ATACAGGACTCTTTCGAGGCCCTGT	3474	0.10124722947186192	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	2.9144280216425426E-5	0.0
5	0.0	2.9144280216425426E-5	0.0	2.9144280216425426E-5	0.0
6	0.0	2.9144280216425426E-5	0.0	2.9144280216425426E-5	0.0
7	0.0	2.9144280216425426E-5	0.0	2.9144280216425426E-5	0.0
8	0.0	2.9144280216425426E-5	0.0	2.9144280216425426E-5	0.0
9	0.0	2.9144280216425426E-5	0.0	1.4572140108212711E-4	0.0
10	0.0	2.9144280216425426E-5	0.0	1.7486568129855255E-4	0.0
11	0.0	2.9144280216425426E-5	0.0	1.7486568129855255E-4	2.9144280216425426E-5
12	0.0	2.9144280216425426E-5	0.0	2.0400996151497797E-4	2.9144280216425426E-5
13	0.0	2.9144280216425426E-5	0.0	2.0400996151497797E-4	2.9144280216425426E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1475	0.0	16.040684	1
ATCGCCA	540	0.0	14.24	16
CGCCAGT	560	0.0	13.561904	18
CGCATCG	540	0.0	13.537579	13
GTATCAA	5025	0.0	13.327979	1
GCATCGC	575	0.0	13.043774	14
CGTCGTA	470	0.0	12.927845	10
CATCGCC	585	0.0	12.820056	15
CGCGCCA	150	1.8189894E-12	12.658515	10
TCGCCAG	595	0.0	12.604593	17
GCCGCGC	205	0.0	12.504143	8
CCGCATC	595	0.0	12.445766	12
CCGTCGT	520	0.0	12.0499325	9
GCGTTAT	175	0.0	11.993553	1
GTCGTAG	520	0.0	11.867358	11
CGTTATT	170	1.8189894E-12	11.749832	2
GCGTAAC	255	0.0	11.541587	11
ACCGTCG	585	0.0	11.360207	8
GCTCGTA	310	0.0	11.331412	9
CGCGTAA	260	0.0	11.3196335	10
>>END_MODULE