FastQCFastQC Report
Thu 2 Feb 2017
SRR4050217_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4050217_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3957313
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNN88000.22237311023919512No Hit
GNNNNNNNNNNNNNNNNNNNNNNNN65600.16576904581467275No Hit
GTATCAACGCAGAGTACTTTTTTTT63040.1593000098804416No Hit
GTATCAACGCAGAGTACATGGGGAA44080.11138871249254229No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA19050.016.7593061
GTATCAA60800.013.1432911
GCGTTAT1900.012.0024361
CTCGTAG2600.011.69125810
CGGTCCA5200.011.50858210
GCTCGTA2500.011.3991229
CGAACGT2000.010.9258274
CGCGCTA2000.010.92457716
ATTCGAA1951.8189894E-1210.7201241
ACCGTCC1355.7034413E-810.5546088
CCGTCGT4600.010.5317979
AGCCCCG6500.010.52266816
ACTGTTC16450.010.5096968
CGTTATT2450.010.4712462
ACTGCGC1002.4033125E-510.4490628
CGTCGTA4650.010.4184210
GATATAC16300.010.3180451
TATACTG4450.010.2477055
AGGCCCG5850.010.22985210
TGGCGAG23200.010.19598518
Produced by FastQC (version 0.11.5)