##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR4050217_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3957313 Sequences flagged as poor quality 0 Sequence length 25 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.864893931816866 31.0 30.0 34.0 26.0 34.0 2 29.992029945571655 31.0 31.0 34.0 26.0 34.0 3 30.110166418476375 31.0 31.0 34.0 26.0 34.0 4 33.50747009397538 37.0 35.0 37.0 28.0 37.0 5 33.57844451525568 37.0 35.0 37.0 28.0 37.0 6 33.67166610273183 37.0 35.0 37.0 29.0 37.0 7 33.59809370651247 37.0 35.0 37.0 28.0 37.0 8 33.522627348405344 37.0 35.0 37.0 28.0 37.0 9 35.01830358124313 39.0 35.0 39.0 28.0 39.0 10 34.89772327839623 39.0 35.0 39.0 28.0 39.0 11 34.93511961272712 39.0 35.0 39.0 28.0 39.0 12 34.82655276446417 39.0 35.0 39.0 27.0 39.0 13 34.833436981103084 39.0 35.0 39.0 27.0 39.0 14 35.66113067124081 40.0 36.0 41.0 27.0 41.0 15 35.79112796991292 40.0 36.0 41.0 27.0 41.0 16 35.814700277688424 40.0 36.0 41.0 27.0 41.0 17 35.81097578078863 40.0 36.0 41.0 27.0 41.0 18 35.647065572018185 40.0 36.0 41.0 27.0 41.0 19 35.78829801938841 40.0 36.0 41.0 27.0 41.0 20 35.753440781661695 40.0 36.0 41.0 27.0 41.0 21 35.71359379457728 40.0 36.0 41.0 27.0 41.0 22 35.63515547039115 40.0 36.0 41.0 27.0 41.0 23 35.529359947014555 40.0 36.0 41.0 27.0 41.0 24 35.456007396938276 40.0 36.0 41.0 26.0 41.0 25 35.17294184210347 39.0 36.0 41.0 25.0 41.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 1.0887063747183134 1101 2 1.1012778917368529 1101 3 1.0101983314877678 1101 4 1.1207444910128288 1101 5 0.9978134592462666 1101 6 1.0404992330107419 1101 7 1.1148314204278194 1101 8 1.1313244575393355 1101 9 1.2631418326395476 1101 10 1.3327848079753721 1101 11 1.2192521717869838 1101 12 1.266087114042456 1101 13 1.3176088576849878 1101 14 1.7469940739149195 1101 15 1.6178468830965969 1101 16 1.6054698342958744 1101 17 1.630065690464285 1101 18 1.7516897976904602 1101 19 1.5963508281157957 1101 20 1.6230967344312361 1101 21 1.5619452735921655 1101 22 1.6687113981450992 1101 23 1.6618396638347477 1101 24 1.6489387105993885 1101 25 1.792930741816889 1102 1 0.9054284516368476 1102 2 0.9198287689678324 1102 3 0.8583802382734831 1102 4 0.931959696000483 1102 5 0.9055911442563627 1102 6 0.8763423008371518 1102 7 0.9300439187728458 1102 8 0.8654592104743557 1102 9 1.060605944647321 1102 10 1.0883704373379075 1102 11 1.13433573081943 1102 12 1.1084961657149464 1102 13 1.098229079882607 1102 14 1.555507002954279 1102 15 1.3212120209945866 1102 16 1.344644958056307 1102 17 1.2710210919944416 1102 18 1.4156050254197865 1102 19 1.3741583497736514 1102 20 1.3731691339775196 1102 21 1.3605358841133466 1102 22 1.4259172402732148 1102 23 1.3659035053878554 1102 24 1.3140187238880685 1102 25 1.354779342400903 1103 1 0.8016238056548666 1103 2 0.8054005754070772 1103 3 0.7791612758351434 1103 4 0.9075078572012671 1103 5 0.8563357118740313 1103 6 0.8077009715992389 1103 7 0.8110513428745989 1103 8 0.8327806012935142 1103 9 0.9663129719295611 1103 10 1.0409222588609168 1103 11 1.0334015997931871 1103 12 1.0714459279547697 1103 13 1.0473877688705002 1103 14 1.4351449257651439 1103 15 1.3430261154092733 1103 16 1.2639000255892583 1103 17 1.250122119786802 1103 18 1.3340475950456252 1103 19 1.2612309366407075 1103 20 1.2471833117911117 1103 21 1.2592447671294735 1103 22 1.323327992534324 1103 23 1.199026731016211 1103 24 1.3019236504708758 1103 25 1.3576056755963464 1104 1 0.8052288241058676 1104 2 0.8413995622995394 1104 3 0.762758618426588 1104 4 0.868383577317509 1104 5 0.8416459048089777 1104 6 0.8267497640006596 1104 7 0.86559556333507 1104 8 0.8408491440586232 1104 9 0.9385430878183953 1104 10 0.9931981663528759 1104 11 1.022226521784603 1104 12 1.0680697540271282 1104 13 1.02351858206206 1104 14 1.4334380022394413 1104 15 1.338609848009419 1104 16 1.2924582710443815 1104 17 1.302421810211456 1104 18 1.415235352030713 1104 19 1.312491321733802 1104 20 1.3300548209663603 1104 21 1.2211934619464628 1104 22 1.228072996557934 1104 23 1.2020543576163476 1104 24 1.2830119945450988 1104 25 1.2909357533833088 1105 1 0.5459968834389315 1105 2 0.6200702168334828 1105 3 0.5144570583390191 1105 4 0.6333376126198473 1105 5 0.6078909093044231 1105 6 0.5246263970093494 1105 7 0.583712565051961 1105 8 0.5896096491325054 1105 9 0.6649806328866248 1105 10 0.7480769838469072 1105 11 0.5860238518232563 1105 12 0.6128041910980997 1105 13 0.6162307417571995 1105 14 0.9848958492888329 1105 15 0.86502492938817 1105 16 0.8688549108669008 1105 17 0.8463853534752346 1105 18 0.9349562479197644 1105 19 0.8157228873631723 1105 20 0.7976543835269823 1105 21 0.7548271426816413 1105 22 0.7861892667587611 1105 23 0.7603508254120754 1105 24 0.7621300729885618 1105 25 0.8144900284710701 1106 1 0.5537972382745373 1106 2 0.4326209664239187 1106 3 0.4084493057893823 1106 4 0.5880702302689187 1106 5 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0.6315881930917726 1107 18 0.6906665632068538 1107 19 0.6695257171029638 1107 20 0.6632031855506142 1107 21 0.6506459362713954 1107 22 0.7218190030023948 1107 23 0.7028121976903421 1107 24 0.6637575657666872 1107 25 0.6424573451827911 1108 1 0.36719302433505874 1108 2 0.3382218099365204 1108 3 0.3457272207913782 1108 4 0.4685687229375972 1108 5 0.243487706702588 1108 6 0.3633890596589566 1108 7 0.34019205328031177 1108 8 0.3428969997170199 1108 9 0.3956143823067535 1108 10 0.48096884399384265 1108 11 0.4804376529152279 1108 12 0.49070659097901626 1108 13 0.5135409412091434 1108 14 0.8415413935862119 1108 15 0.6542817592309191 1108 16 0.6501221914700395 1108 17 0.5889262883247426 1108 18 0.7339832740696082 1108 19 0.6638150472224282 1108 20 0.6707658349885293 1108 21 0.6433355870594326 1108 22 0.6574876713334632 1108 23 0.7371808975926086 1108 24 0.7648318485993926 1108 25 0.7163879919829483 1109 1 0.16830236094057582 1109 2 0.24615904909369846 1109 3 0.19380692015949919 1109 4 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2215 13 -1.9523022712717975 2215 14 -2.7344215908285534 2215 15 -2.359802650177123 2215 16 -2.13875238518105 2215 17 -2.14271996951485 2215 18 -2.0154142432495945 2215 19 -2.226137979224106 2215 20 -2.4664900766795483 2215 21 -2.317739283200524 2215 22 -2.6017207441013426 2215 23 -2.272794340330563 2215 24 -2.2926598390042585 2215 25 -2.2200050390817196 2216 1 -1.937001159431965 2216 2 -1.9075247482518982 2216 3 -1.868256373302195 2216 4 -1.6891471216945178 2216 5 -2.222861550522044 2216 6 -1.8881327353752901 2216 7 -2.064475542632003 2216 8 -2.4257267974192125 2216 9 -2.456258768208677 2216 10 -2.557244861385591 2216 11 -2.4067656841698692 2216 12 -2.601468645121429 2216 13 -2.5284027599216046 2216 14 -2.480935201349645 2216 15 -2.4386395132889973 2216 16 -2.553741414400605 2216 17 -2.5914891142945393 2216 18 -2.9896968368435637 2216 19 -2.713772647229675 2216 20 -2.8482122991657235 2216 21 -3.0002008137783776 2216 22 -3.2322450338894484 2216 23 -2.9706527177444144 2216 24 -3.177270619367725 2216 25 -3.0825448973498624 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 147331.0 3 11.0 4 1044.0 5 6330.0 6 8008.0 7 7185.0 8 6771.0 9 6228.0 10 5794.0 11 5709.0 12 5532.0 13 5337.0 14 5361.0 15 5590.0 16 5753.0 17 5959.0 18 6909.0 19 8095.0 20 10612.0 21 13647.0 22 17026.0 23 20492.0 24 24251.0 25 27878.0 26 31697.0 27 35812.0 28 40494.0 29 49220.0 30 61406.0 31 78505.0 32 100964.0 33 132368.0 34 178199.0 35 246813.0 36 359578.0 37 621758.0 38 1663646.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.00758350543246 18.79208450408859 13.032247247300488 25.168084743178465 2 15.248391906976675 20.64201131737351 40.19333486103548 23.91626191461434 3 18.15908826755627 25.590808412024284 29.652286414121466 26.597816906297982 4 12.061036044972536 17.30192255453444 38.424714953846276 32.21232644664674 5 13.794148188435477 37.2560367253096 34.41105137544384 14.53876371081108 6 30.822333780363103 37.81062780840666 18.37409395054482 12.992944460685424 7 27.507344330344885 31.717534014674158 22.42194096906235 18.353180685918602 8 25.62919333965438 34.82020267188224 20.77062430500559 18.77997968345779 9 26.91712143886847 15.038021270950619 20.05451924390417 37.99033804627675 10 15.588803248408242 27.853696471131123 32.73790883973754 23.819591440723087 11 34.67999206276521 22.44700878183798 24.322180500859865 18.550818654536943 12 23.87570725815212 24.751523144236778 30.518775969262617 20.853993628348483 13 28.57321654930518 21.366175725009583 25.945143457787132 24.115464267898105 14 22.37785860604394 21.75731696056438 26.87193338086707 28.992891052524612 15 23.761575293016897 29.37301990688379 23.3462358960896 23.519168904009717 16 23.64040686921254 27.364300182817402 26.018839402060394 22.97645354590966 17 22.496155250293523 27.342765955552277 26.663749448251384 23.497329345902813 18 22.660873608974203 26.68777627176594 28.522541333919456 22.128808785340397 19 24.64565785393449 25.797420870985803 26.597773553256065 22.95914772182364 20 24.73871292231934 26.19770251561159 26.34894653263233 22.714638029436742 21 25.712275970846 25.506315427960875 25.457903506875102 23.323505094318023 22 24.725662334503824 26.22720233965795 26.152739713307437 22.894395612530786 23 23.556386392378574 25.92936795880845 26.450546199171704 24.063699449641273 24 23.716317657585826 26.51028149280411 26.638127530047633 23.135273319562426 25 23.792240115714986 26.038033720355795 26.75013571479085 23.41959044913837 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 14580.0 1 14580.0 2 13094.5 3 11609.0 4 11609.0 5 11609.0 6 7933.0 7 4257.0 8 4257.0 9 4257.0 10 6103.0 11 7949.0 12 7949.0 13 7949.0 14 13143.0 15 18337.0 16 18337.0 17 18337.0 18 31259.0 19 44181.0 20 44181.0 21 44181.0 22 70161.5 23 96142.0 24 96142.0 25 96142.0 26 138776.5 27 181411.0 28 181411.0 29 181411.0 30 227068.0 31 272725.0 32 272725.0 33 272725.0 34 306008.5 35 339292.0 36 339292.0 37 339292.0 38 364058.5 39 388825.0 40 388825.0 41 388825.0 42 417757.5 43 446690.0 44 446690.0 45 446690.0 46 467954.5 47 489219.0 48 489219.0 49 489219.0 50 481030.5 51 472842.0 52 472842.0 53 472842.0 54 422141.5 55 371441.0 56 371441.0 57 371441.0 58 330729.5 59 290018.0 60 290018.0 61 290018.0 62 251855.5 63 213693.0 64 213693.0 65 213693.0 66 175520.5 67 137348.0 68 137348.0 69 137348.0 70 104619.5 71 71891.0 72 71891.0 73 71891.0 74 56700.0 75 41509.0 76 41509.0 77 41509.0 78 33112.0 79 24715.0 80 24715.0 81 24715.0 82 17885.5 83 11056.0 84 11056.0 85 11056.0 86 8143.0 87 5230.0 88 5230.0 89 5230.0 90 3592.5 91 1955.0 92 1955.0 93 1955.0 94 1138.5 95 322.0 96 322.0 97 322.0 98 199.0 99 76.0 100 76.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.22760393226413986 2 0.6439217721721785 3 0.6410915689509523 4 0.6486472007647613 5 0.6643649365112135 6 0.6804111779887009 7 0.6754583223515552 8 0.6703033093414648 9 0.6626718685128016 10 0.6640869701234146 11 0.6689640167457059 12 0.6687365897011432 13 0.664491284869304 14 0.6587803390836156 15 0.6648703299435754 16 0.6725523101154749 17 0.6728808158465099 18 0.6671446003892034 19 0.664491284869304 20 0.6716426019372236 21 0.668711320029525 22 0.6691914437902688 23 0.6722743437276758 24 0.6773282780512939 25 0.6639606217653241 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 3957313.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.63344230340398 #Duplication Level Percentage of deduplicated Percentage of total 1 88.96122919115918 53.940255574020476 2 6.809070763411793 8.257147985462478 3 1.6010767706643096 2.9123638799218443 4 0.7103683943895296 1.722883242215171 5 0.41314505682688357 1.2525203483024705 6 0.27360515442645306 0.9953773406898168 7 0.18385878059054492 0.7803593535437714 8 0.1497320766322933 0.7263016983562416 9 0.10178154298640708 0.5554228782795972 >10 0.6597655395378942 7.433069483061729 >50 0.059450493291660904 2.545402784314689 >100 0.060683172716814116 7.975702355169513 >500 0.010245803657501978 4.2561217898425605 >1k 0.005860768306501154 6.093223245229154 >5k 1.2649140229858605E-4 0.5538480415905261 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source NNNNNNNNNNNNNNNNNNNNNNNNN 8800 0.22237311023919512 No Hit GNNNNNNNNNNNNNNNNNNNNNNNN 6560 0.16576904581467275 No Hit GTATCAACGCAGAGTACTTTTTTTT 6304 0.1593000098804416 No Hit GTATCAACGCAGAGTACATGGGGAA 4408 0.11138871249254229 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 5.0539343236180714E-5 2 0.0 0.0 0.0 0.0 5.0539343236180714E-5 3 0.0 0.0 0.0 0.0 5.0539343236180714E-5 4 0.0 0.0 0.0 2.5269671618090357E-5 5.0539343236180714E-5 5 2.5269671618090357E-5 0.0 0.0 5.0539343236180714E-5 5.0539343236180714E-5 6 2.5269671618090357E-5 0.0 0.0 7.580901485427107E-5 5.0539343236180714E-5 7 2.5269671618090357E-5 0.0 0.0 1.2634835809045177E-4 5.0539343236180714E-5 8 2.5269671618090357E-5 0.0 0.0 1.2634835809045177E-4 5.0539343236180714E-5 9 2.5269671618090357E-5 0.0 0.0 1.7688770132663249E-4 7.580901485427107E-5 10 2.5269671618090357E-5 0.0 0.0 1.7688770132663249E-4 7.580901485427107E-5 11 2.5269671618090357E-5 0.0 0.0 2.274270445628132E-4 7.580901485427107E-5 12 2.5269671618090357E-5 0.0 0.0 2.274270445628132E-4 1.0107868647236143E-4 13 2.5269671618090357E-5 0.0 0.0 2.5269671618090354E-4 1.0107868647236143E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1905 0.0 16.759306 1 GTATCAA 6080 0.0 13.143291 1 GCGTTAT 190 0.0 12.002436 1 CTCGTAG 260 0.0 11.691258 10 CGGTCCA 520 0.0 11.508582 10 GCTCGTA 250 0.0 11.399122 9 CGAACGT 200 0.0 10.925827 4 CGCGCTA 200 0.0 10.924577 16 ATTCGAA 195 1.8189894E-12 10.720124 1 ACCGTCC 135 5.7034413E-8 10.554608 8 CCGTCGT 460 0.0 10.531797 9 AGCCCCG 650 0.0 10.522668 16 ACTGTTC 1645 0.0 10.509696 8 CGTTATT 245 0.0 10.471246 2 ACTGCGC 100 2.4033125E-5 10.449062 8 CGTCGTA 465 0.0 10.41842 10 GATATAC 1630 0.0 10.318045 1 TATACTG 445 0.0 10.247705 5 AGGCCCG 585 0.0 10.229852 10 TGGCGAG 2320 0.0 10.195985 18 >>END_MODULE