FastQCFastQC Report
Thu 2 Feb 2017
SRR4050217_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4050217_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3957313
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT100580.2541623571347528No Hit
TATCAACGCAGAGTACTTTTTTTTT62060.15682358206186875No Hit
GTATCAACGCAGAGTACATGGGGAA41810.10565249703523578No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA17900.016.292341
GTATCAA59950.013.5127821
CGCATCG4600.012.38880813
GCATCGC4650.012.25512914
CGTCGTA5300.012.00701310
ATCGCCA4750.011.99606616
ACCGTCG5400.011.7842158
CGAACGA2100.011.75804916
CCGTCGT5450.011.6765449
TCCAACG5550.011.635529518
CGCCAGT4850.011.5526218
CATCGCC5150.011.434154515
CGTCTTA3250.011.3972715
ATACCGT5550.011.29373556
CAACGCT600.005872819611.0847434
CCAACGA3700.011.03664219
TCGCCAG5050.010.90703817
TACCGTC5900.010.7855527
TCGCGTA2400.010.6853479
CGAGCCG6400.010.6849415
Produced by FastQC (version 0.11.5)