##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4050217_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3957313
Sequences flagged as poor quality	0
Sequence length	25
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.856554182092747	31.0	31.0	34.0	27.0	34.0
2	30.829275318884303	31.0	31.0	34.0	27.0	34.0
3	31.051833908513174	31.0	31.0	34.0	27.0	34.0
4	34.68148463363904	37.0	35.0	37.0	32.0	37.0
5	34.61722790186169	37.0	35.0	37.0	32.0	37.0
6	34.56642524864725	37.0	35.0	37.0	31.0	37.0
7	34.516666485567356	37.0	35.0	37.0	31.0	37.0
8	34.48910687630723	37.0	35.0	37.0	31.0	37.0
9	36.07648674744707	39.0	37.0	39.0	31.0	39.0
10	35.9556196338273	39.0	37.0	39.0	31.0	39.0
11	35.954103706226924	39.0	37.0	39.0	31.0	39.0
12	35.885185478126196	39.0	37.0	39.0	30.0	39.0
13	35.89346356985156	39.0	37.0	39.0	31.0	39.0
14	37.0472335647951	40.0	37.0	41.0	31.0	41.0
15	37.02604317626632	40.0	37.0	41.0	31.0	41.0
16	37.00874002132255	40.0	37.0	41.0	31.0	41.0
17	36.9913810204045	40.0	37.0	41.0	31.0	41.0
18	36.95745446468349	40.0	37.0	41.0	31.0	41.0
19	37.01910715680059	40.0	37.0	41.0	31.0	41.0
20	36.97561906273272	40.0	37.0	41.0	31.0	41.0
21	36.92432693597903	40.0	37.0	41.0	31.0	41.0
22	36.86451589752946	40.0	37.0	41.0	31.0	41.0
23	36.76699417003406	40.0	37.0	41.0	31.0	41.0
24	36.71414290454154	40.0	37.0	41.0	31.0	41.0
25	36.49921878810193	40.0	37.0	41.0	30.0	41.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.7500349532101218
1101	2	0.8990187619280867
1101	3	0.8258794588676679
1101	4	0.7471163494246085
1101	5	0.8049241623330232
1101	6	0.8599783352127233
1101	7	0.8588385141857486
1101	8	0.8159655058199391
1101	9	0.9460903160006993
1101	10	0.9511844678025341
1101	11	0.9168127709420588
1101	12	0.9750511050286974
1101	13	0.9060387426227408
1101	14	1.1343640655582874
1101	15	1.1576134616889533
1101	16	1.1635569130682626
1101	17	1.187034511443379
1101	18	1.2146931950051965
1101	19	1.1485753100958789
1101	20	1.230769798728005
1101	21	1.2000821353560767
1101	22	1.180516953838584
1101	23	1.2497231731199463
1101	24	1.220622503549592
1101	25	1.343963384109017
1102	1	0.5970672704811797
1102	2	0.799841968349412
1102	3	0.6796108913097214
1102	4	0.6019464384315114
1102	5	0.7296940295894601
1102	6	0.7683734337721759
1102	7	0.7146032472750861
1102	8	0.7273195362826002
1102	9	0.8004513537612965
1102	10	0.8953073887827543
1102	11	0.8891943265070097
1102	12	0.8908048270989823
1102	13	0.9685026956833838
1102	14	1.0238143425280342
1102	15	1.0620185679791732
1102	16	1.0763166999323772
1102	17	1.0504819804635375
1102	18	1.1100748899545536
1102	19	1.1352831376658372
1102	20	1.0829846659093363
1102	21	1.1626454740423782
1102	22	1.1436695497247484
1102	23	1.158562380452338
1102	24	1.1184033919557805
1102	25	1.1321581533858947
1103	1	0.4794752830399922
1103	2	0.6381739103127018
1103	3	0.5582351659216869
1103	4	0.45002449020913815
1103	5	0.5518420861334477
1103	6	0.6606667320222144
1103	7	0.6409616489815804
1103	8	0.6629905600949328
1103	9	0.7643203031966763
1103	10	0.7324996012643652
1103	11	0.683743176923258
1103	12	0.6960190411642699
1103	13	0.7673782265683826
1103	14	0.8603003798756177
1103	15	0.8815867562454471
1103	16	0.8979741923309348
1103	17	0.8561986870831504
1103	18	0.8284598167106267
1103	19	0.8029736594287726
1103	20	0.8662277837088723
1103	21	0.8711623413289971
1103	22	0.9142846474585724
1103	23	0.9241191997454408
1103	24	0.897231132570198
1103	25	0.9546856532830219
1104	1	0.5978643827897869
1104	2	0.7189729563220233
1104	3	0.6423856067161502
1104	4	0.6171306136018302
1104	5	0.6658005743232849
1104	6	0.7429574130533041
1104	7	0.7092081677800266
1104	8	0.7049655762880747
1104	9	0.7735528036208521
1104	10	0.7643844308365146
1104	11	0.7963709053637871
1104	12	0.845076144351367
1104	13	0.8664670539935102
1104	14	0.9179159845059246
1104	15	0.9882684993074307
1104	16	1.0080275381046988
1104	17	0.8995115878172157
1104	18	0.9366877656539927
1104	19	0.9617895518502166
1104	20	1.0015478157918736
1104	21	0.9567623619593704
1104	22	0.9328027059364601
1104	23	1.0119715057157705
1104	24	1.0034269711496577
1104	25	1.0756681456524007
1105	1	0.44477593580864294
1105	2	0.5380894596559322
1105	3	0.4349233414061189
1105	4	0.4468364447188691
1105	5	0.5037282182251204
1105	6	0.6206678412728053
1105	7	0.5227213725187809
1105	8	0.5496241923813869
1105	9	0.6474352456143109
1105	10	0.651052104593127
1105	11	0.6682169729316456
1105	12	0.6410126569515526
1105	13	0.6149200089840008
1105	14	0.754103686708504
1105	15	0.7938718702106016
1105	16	0.7541550975146194
1105	17	0.7224844884007453
1105	18	0.7342782035458981
1105	19	0.7066548242456534
1105	20	0.725669305064585
1105	21	0.648840976918656
1105	22	0.7030675237916739
1105	23	0.6916094365954564
1105	24	0.7230977816881392
1105	25	0.7265341455869603
1106	1	0.35993080047403936
1106	2	0.5014711119512647
1106	3	0.4443502022466319
1106	4	0.39362184625633034
1106	5	0.47238622090655724
1106	6	0.5191050745673564
1106	7	0.5116609695357255
1106	8	0.43079624960296314
1106	9	0.519417904401621
1106	10	0.5132730345081811
1106	11	0.4737340750459538
1106	12	0.5625668743154506
1106	13	0.6177343771708337
1106	14	0.6533320945901053
1106	15	0.6873272864194249
1106	16	0.5763927265296616
1106	17	0.5731845971249996
1106	18	0.5394930123397259
1106	19	0.6280966950498268
1106	20	0.610461759128377
1106	21	0.7015741066711456
1106	22	0.6146864831770316
1106	23	0.6557355875360145
1106	24	0.6953467915834111
1106	25	0.6848312763513817
1107	1	0.32020878945664677
1107	2	0.46647814076496985
1107	3	0.4037134390614803
1107	4	0.434327439572904
1107	5	0.4157240245278615
1107	6	0.5484895469692077
1107	7	0.4412879447918385
1107	8	0.49154950381296203
1107	9	0.47362559989466746
1107	10	0.5045802265906403
1107	11	0.5424430219427094
1107	12	0.5779245520866141
1107	13	0.594381002161029
1107	14	0.5995157690642259
1107	15	0.6838551700938851
1107	16	0.6573682120190512
1107	17	0.5715757450738721
1107	18	0.6027691459474553
1107	19	0.6009677545526841
1107	20	0.7086591902618693
1107	21	0.6703542756091139
1107	22	0.6775325469437874
1107	23	0.7130730105704615
1107	24	0.6440645801830769
1107	25	0.5969971986174372
1108	1	0.20030875055833874
1108	2	0.3729197970785094
1108	3	0.27681423739262456
1108	4	0.22777760071532782
1108	5	0.2794644467630363
1108	6	0.35543483397081843
1108	7	0.30820994668611235
1108	8	0.31327302198688045
1108	9	0.37675254952405623
1108	10	0.38090117963144365
1108	11	0.4327475924396964
1108	12	0.38007209411241405
1108	13	0.39551497934571955
1108	14	0.3497673150294176
1108	15	0.3612925368187021
1108	16	0.38353233269646125
1108	17	0.4537941014103666
1108	18	0.3905198972450563
1108	19	0.34160096724021116
1108	20	0.37418036828788104
1108	21	0.4238979004354988
1108	22	0.41512641200222333
1108	23	0.4637377298076615
1108	24	0.4682550159410681
1108	25	0.5555912996517449
1109	1	0.1268193977646348
1109	2	0.2237950164015281
1109	3	0.1982540534981112
1109	4	0.2390677227071194
1109	5	0.31780952050635847
1109	6	0.3267651051592608
1109	7	0.29630561660457744
1109	8	0.23768542284458505
1109	9	0.3702006856540834
1109	10	0.3094396514539355
1109	11	0.34093615271686417
1109	12	0.3193841582516299
1109	13	0.3895032745179705
1109	14	0.43839899848709507
1109	15	0.5252008548741287
1109	16	0.4289840422500859
1109	17	0.32544108902878577
1109	18	0.3944837754045736
1109	19	0.3574395869535323
1109	20	0.3485546823366761
1109	21	0.40818079577916677
1109	22	0.4715061856784857
1109	23	0.5667693879777786
1109	24	0.6025454768277356
1109	25	0.5863221207411584
1110	1	-0.24155394419944543
1110	2	-0.10074768659473321
1110	3	-0.14667844565161303
1110	4	-0.1612128679870395
1110	5	-0.04341157014939512
1110	6	-0.2577450131112258
1110	7	-0.1476972715870275
1110	8	-0.1682573810875283
1110	9	-0.14713977810226453
1110	10	-0.13492494639349673
1110	11	-0.1751155275359153
1110	12	-0.12381062599798298
1110	13	-0.13654804916794205
1110	14	-0.17399293432473684
1110	15	-0.17741552306480912
1110	16	-0.11107281645070088
1110	17	-0.1830239454759166
1110	18	-0.2345197162078705
1110	19	-0.1253882899925003
1110	20	-0.11431367866258313
1110	21	-0.22551120724890694
1110	22	-0.23999796421124842
1110	23	-0.16933478988874384
1110	24	-0.19523722583938508
1110	25	-0.11298881379316583
1111	1	-0.35055218445463865
1111	2	-0.21753033309790126
1111	3	-0.21410970378086702
1111	4	-0.10556682388159544
1111	5	-0.17056435160557015
1111	6	-0.41433894316872966
1111	7	-0.33059283897230074
1111	8	-0.2494794988432929
1111	9	-0.4084830328062026
1111	10	-0.3343440540439673
1111	11	-0.34781292499693706
1111	12	-0.24278320576033252
1111	13	-0.24848532809632218
1111	14	-0.2922647795848974
1111	15	-0.29260818275135136
1111	16	-0.3452421021355576
1111	17	-0.36033110922635103
1111	18	-0.3989129500185129
1111	19	-0.4544270956024974
1111	20	-0.41450576451929777
1111	21	-0.3650739783467216
1111	22	-0.46825294034581333
1111	23	-0.42011718478960347
1111	24	-0.41396938561715046
1111	25	-0.41016958048867025
1112	1	-0.4547186530538099
1112	2	-0.3849896658798144
1112	3	-0.40560175414388766
1112	4	-0.3167273726394697
1112	5	-0.4043608459693644
1112	6	-1.3045548842745447
1112	7	-0.6215654771362651
1112	8	-0.5773204205384985
1112	9	-0.5782308374260836
1112	10	-0.5417920256743685
1112	11	-0.5836114856008408
1112	12	-0.6366574534999856
1112	13	-0.49766968615165297
1112	14	-0.604566910157331
1112	15	-0.5744356485332105
1112	16	-0.6088913293554157
1112	17	-0.6706323619636478
1112	18	-0.6898729118627926
1112	19	-0.5990920287824508
1112	20	-0.591122549868011
1112	21	-0.598248144641019
1112	22	-0.6933538755412982
1112	23	-0.6523065594984487
1112	24	-0.6113460212585267
1112	25	-0.6693456514000005
1113	1	-0.5654659878036732
1113	2	-0.64608735237017
1113	3	-0.541906403537542
1113	4	-0.43437295647931506
1113	5	-0.46391601267383464
1113	6	-1.0011642416981275
1113	7	-0.5775504662499742
1113	8	-0.5133621325325493
1113	9	-0.5933022888127581
1113	10	-0.48304735115969777
1113	11	-0.6480249762645585
1113	12	-0.7421522124971816
1113	13	-0.6551069260496263
1113	14	-0.7501711955431674
1113	15	-0.7193042801322989
1113	16	-0.7948126826806359
1113	17	-0.8286860542744208
1113	18	-0.7851707455610608
1113	19	-0.7940711434672849
1113	20	-0.8180896811499991
1113	21	-0.8624037900026593
1113	22	-0.9471752632767547
1113	23	-0.8768179110182857
1113	24	-0.9318279805742122
1113	25	-0.9614403242944576
1114	1	-0.85800230040579
1114	2	-0.9061163475569565
1114	3	-1.0110783366846903
1114	4	-0.8396832385247919
1114	5	-1.0247410984280734
1114	6	-1.5579402559769093
1114	7	-1.2012534786844355
1114	8	-1.2568603259562892
1114	9	-1.4424318232657924
1114	10	-1.5599461623036674
1114	11	-1.3056031043023637
1114	12	-1.3539348332259564
1114	13	-1.480105191522938
1114	14	-1.6512332214550867
1114	15	-1.7285502604143517
1114	16	-1.6062782090580185
1114	17	-1.6623462791758215
1114	18	-1.7516234745624217
1114	19	-1.6050784498273956
1114	20	-1.5885542369548702
1114	21	-1.5265430034958314
1114	22	-1.4928220968696948
1114	23	-1.5684076295065097
1114	24	-1.6244187687507647
1114	25	-1.6319200905641296
1115	1	-1.2829591378526715
1115	2	-1.8391603612080658
1115	3	-1.4839364241184292
1115	4	-1.1694899468234894
1115	5	-1.2471879502445233
1115	6	-1.819312773020684
1115	7	-1.4233384414549661
1115	8	-1.4817188703965343
1115	9	-1.5538412060401114
1115	10	-1.5677233820192953
1115	11	-1.6231951048496072
1115	12	-1.4518664403466275
1115	13	-1.5314092584764296
1115	14	-1.7118971315245872
1115	15	-1.7059672601623888
1115	16	-1.8659801153276732
1115	17	-1.7784785449290226
1115	18	-1.8956720652171057
1115	19	-1.8407495655392196
1115	20	-1.8674339852879953
1115	21	-2.0531195535538984
1115	22	-2.06729172392199
1115	23	-1.9923621685971895
1115	24	-2.0478550038923586
1115	25	-2.1013161706861894
1116	1	-1.0044665262784704
1116	2	-1.0873787492344924
1116	3	-1.1497986104384132
1116	4	-1.0048314800382343
1116	5	-1.0069128864208636
1116	6	-1.2249825994274701
1116	7	-1.2568177821855357
1116	8	-1.2466693330511376
1116	9	-1.3924779833257972
1116	10	-1.4245121866246535
1116	11	-1.435459466673052
1116	12	-1.372933795109482
1116	13	-1.4739140300430549
1116	14	-1.6800661671311943
1116	15	-1.6506861911238317
1116	16	-1.5565155511242708
1116	17	-1.6009104071523268
1116	18	-1.6962964161526557
1116	19	-1.7126008425476797
1116	20	-1.6975138585393523
1116	21	-1.8128785549687407
1116	22	-1.7319412688590674
1116	23	-1.730257451080746
1116	24	-1.7709426543998816
1116	25	-1.9670625919706666
1201	1	0.7933071751548191
1201	2	0.9623851814950335
1201	3	0.8596865051202762
1201	4	0.7760641547179077
1201	5	0.8569841424217444
1201	6	0.8610077071293389
1201	7	0.836056091619497
1201	8	0.8363828959483683
1201	9	1.0687803473914954
1201	10	1.0970373856934756
1201	11	1.0343640162550116
1201	12	1.1133918786016963
1201	13	1.1119335620058166
1201	14	1.288171404475456
1201	15	1.2919669328672967
1201	16	1.2826247158789528
1201	17	1.3232948436378464
1201	18	1.2944902068999937
1201	19	1.3177566213441452
1201	20	1.2429623054050438
1201	21	1.321790478970513
1201	22	1.271586345111423
1201	23	1.2976270640953373
1201	24	1.3252321722785325
1201	25	1.3421026934509186
1202	1	0.6176173643301155
1202	2	0.7554168567703918
1202	3	0.7456933144988795
1202	4	0.6973664657805969
1202	5	0.7787926529088907
1202	6	0.8074396885042177
1202	7	0.8121612125916045
1202	8	0.8211408267020204
1202	9	0.8911786933402155
1202	10	0.9906258554715492
1202	11	0.9683322176532627
1202	12	0.9436040454605958
1202	13	0.9566536650884885
1202	14	1.2070754931999872
1202	15	1.2195003524722736
1202	16	1.1331222625646689
1202	17	1.1796475167657903
1202	18	1.1352872378029133
1202	19	1.1029021564314974
1202	20	1.1312062526793198
1202	21	1.1878930410620825
1202	22	1.1466509920100663
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2211	2	-0.07277637881130161
2211	3	-0.1576798937563133
2211	4	-0.20069474077903493
2211	5	-0.26545621478945947
2211	6	-0.1281004650114781
2211	7	-0.297438307451074
2211	8	-0.18314535566274515
2211	9	-0.18254285080177368
2211	10	-0.2436071185655635
2211	11	-0.26506157831641985
2211	12	-0.22464289208290467
2211	13	-0.26460284442891435
2211	14	-0.3660095346042951
2211	15	-0.3307969488886684
2211	16	-0.3482991479338011
2211	17	-0.4091976872613614
2211	18	-0.3612671165258732
2211	19	-0.282468519797483
2211	20	-0.3818736372538467
2211	21	-0.31130502986997044
2211	22	-0.2852571010087246
2211	23	-0.3320319618431071
2211	24	-0.30589374501307276
2211	25	-0.35142898394734345
2212	1	-0.3758478981061444
2212	2	-0.430875723643954
2212	3	-0.40450033551669407
2212	4	-0.4357778130550045
2212	5	-0.42030516035723764
2212	6	-0.38301763545248946
2212	7	-0.5115652961535346
2212	8	-0.44088480540924024
2212	9	-0.5735464891429203
2212	10	-0.6470451749390165
2212	11	-0.5989652282378515
2212	12	-0.7274496201974969
2212	13	-0.6357358632137959
2212	14	-0.8158701271716069
2212	15	-0.7835017472575672
2212	16	-0.7389015291194028
2212	17	-0.6370146875864506
2212	18	-0.6596408034919179
2212	19	-0.661216795106661
2212	20	-0.6834508240074371
2212	21	-0.6828770567247631
2212	22	-0.7732804071208719
2212	23	-0.8183957987804718
2212	24	-0.9249191448209899
2212	25	-0.8472542519503747
2213	1	-0.5862746112150496
2213	2	-0.9079707240315535
2213	3	-0.6734368614204023
2213	4	-0.6437544654671257
2213	5	-0.7263703936124344
2213	6	-0.6119144020466649
2213	7	-0.662317652040997
2213	8	-0.670326949901991
2213	9	-0.747744663190069
2213	10	-0.7657754735460571
2213	11	-0.6470923714779389
2213	12	-0.6981334771861256
2213	13	-0.7394652778807469
2213	14	-0.8591909413874035
2213	15	-0.9670530606366654
2213	16	-1.0128870121890685
2213	17	-0.9543970918156219
2213	18	-0.9427453644748525
2213	19	-0.855388350049239
2213	20	-0.9614493089780609
2213	21	-0.9839609171930661
2213	22	-0.9736976330256439
2213	23	-0.9811145640171759
2213	24	-0.990253390201211
2213	25	-0.9900450973293928
2214	1	-0.8615673309115586
2214	2	-0.8267002923396731
2214	3	-0.8557881536649852
2214	4	-0.8591074230490321
2214	5	-0.9794214790895026
2214	6	-0.9160493440025164
2214	7	-0.9246583555829702
2214	8	-0.8641976589563427
2214	9	-1.0434165515264908
2214	10	-1.1389485231414582
2214	11	-1.1216288688693439
2214	12	-1.1556973456736799
2214	13	-1.1125931454968097
2214	14	-1.2739309798143452
2214	15	-1.3697763995793224
2214	16	-1.3851093387291087
2214	17	-1.3465583273930193
2214	18	-1.3576609233313164
2214	19	-1.3241831707726135
2214	20	-1.3634725973389408
2214	21	-1.5037878004189125
2214	22	-1.4463450872026158
2214	23	-1.5335809869028267
2214	24	-1.5186385095519697
2214	25	-1.5189914369332698
2215	1	-1.3909744525317507
2215	2	-3.1049062365304785
2215	3	-1.7480772051022093
2215	4	-1.33628504646952
2215	5	-1.2569148410108397
2215	6	-1.192023212762173
2215	7	-1.3298173766184078
2215	8	-1.2796560428268648
2215	9	-1.5002732091225042
2215	10	-1.6325444445192616
2215	11	-1.5873091342610834
2215	12	-1.4334007026255264
2215	13	-1.6416936882506974
2215	14	-1.8662398749579339
2215	15	-1.868964924049152
2215	16	-1.9550676196730166
2215	17	-1.9753081259688798
2215	18	-1.6866967458076232
2215	19	-1.7569115207978143
2215	20	-1.748120065362457
2215	21	-1.7090538009549192
2215	22	-1.8179096089455484
2215	23	-1.9576671436743567
2215	24	-1.93747639523162
2215	25	-2.0053466932288018
2216	1	-1.3314328769135422
2216	2	-2.021471652619237
2216	3	-1.4661019106108633
2216	4	-1.1947050504672276
2216	5	-1.2545885239657224
2216	6	-1.2716768394912705
2216	7	-1.3483463596369845
2216	8	-1.4105487119258697
2216	9	-1.565527506230687
2216	10	-1.590830217442182
2216	11	-1.657030115354786
2216	12	-1.6722643126884762
2216	13	-1.901570847918947
2216	14	-1.8249585699279862
2216	15	-1.9262854490040908
2216	16	-1.8892584416831966
2216	17	-1.8409954766276329
2216	18	-2.1244921407099113
2216	19	-1.9572940006709985
2216	20	-2.0892012348844773
2216	21	-2.0072089392099954
2216	22	-2.0366427574161534
2216	23	-2.120397792222164
2216	24	-2.0157583562516237
2216	25	-2.2074292580447263
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	50115.0
3	5.0
4	989.0
5	8012.0
6	9719.0
7	7689.0
8	6360.0
9	5468.0
10	4905.0
11	4562.0
12	4198.0
13	4017.0
14	4021.0
15	4154.0
16	4359.0
17	4588.0
18	5281.0
19	6086.0
20	8241.0
21	10937.0
22	13867.0
23	17065.0
24	20263.0
25	23823.0
26	27070.0
27	31130.0
28	36834.0
29	44925.0
30	57992.0
31	76387.0
32	99024.0
33	131210.0
34	176918.0
35	243292.0
36	352315.0
37	601968.0
38	1849524.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.34620601530828	19.468697629726158	13.519459874704317	25.665636480261238
2	15.11007000192828	21.244633728854296	39.19842623918627	24.446870030031153
3	17.879935198454103	25.7351642389672	29.866098537062904	26.518802025515797
4	12.418527419994325	17.94086037672532	37.64008558332384	32.000526619956524
5	13.663334996892834	37.58147775659141	34.32345462148459	14.43173262503116
6	30.730613915829803	38.24270573786649	18.084235902982396	12.942444443321321
7	27.344086611150715	31.97778923936004	22.31664409588543	18.361480053603817
8	25.664702111212723	35.014538957000724	20.534511038968752	18.786247892817805
9	26.75666366014	15.082840381738748	19.977118723096368	38.18337723502488
10	15.483646767137344	28.191199253925607	32.57944100022493	23.74571297871211
11	34.649025380071826	22.516344750082197	24.226645853344927	18.607984016501053
12	23.830615079485423	24.973009528546214	30.36857662160168	20.827798770366687
13	28.63109341926516	21.35236470476732	25.898815278172687	24.11772659779484
14	22.51950941968684	21.82353593748697	26.504912196555587	29.1520424462706
15	23.68383473022836	29.482349735739525	23.33443615628206	23.499379377750053
16	23.599738583498613	27.45598579091128	25.90340832125318	23.040867304336928
17	22.42899583835807	27.464031667699413	26.574716989142534	23.53225550479999
18	22.772316007990288	26.7344316397144	28.429752066115704	22.06350028617961
19	24.676767735477576	25.836848288624687	26.630350867129003	22.856033108768735
20	24.780839302708873	26.213211454125222	26.43023677189244	22.57571247127346
21	25.665888933897936	25.494961246872492	25.581233737041753	23.257916082187812
22	24.705627277902963	26.193556082119358	26.22304578889767	22.87777085108001
23	23.51356419110155	25.891593618088017	26.572431304685352	24.022410886125083
24	23.6850863535752	26.457932846300668	26.79587714525641	23.061103654867722
25	23.769446402186585	26.032346420709672	26.844228894858844	23.353978282244896
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	173.0
1	173.0
2	520.0
3	867.0
4	867.0
5	867.0
6	2680.0
7	4493.0
8	4493.0
9	4493.0
10	6334.0
11	8175.0
12	8175.0
13	8175.0
14	13099.0
15	18023.0
16	18023.0
17	18023.0
18	31275.5
19	44528.0
20	44528.0
21	44528.0
22	71221.5
23	97915.0
24	97915.0
25	97915.0
26	142143.5
27	186372.0
28	186372.0
29	186372.0
30	232039.5
31	277707.0
32	277707.0
33	277707.0
34	310538.5
35	343370.0
36	343370.0
37	343370.0
38	367082.0
39	390794.0
40	390794.0
41	390794.0
42	419609.5
43	448425.0
44	448425.0
45	448425.0
46	470646.5
47	492868.0
48	492868.0
49	492868.0
50	483744.5
51	474621.0
52	474621.0
53	474621.0
54	423078.0
55	371535.0
56	371535.0
57	371535.0
58	330945.0
59	290355.0
60	290355.0
61	290355.0
62	252180.0
63	214005.0
64	214005.0
65	214005.0
66	175688.0
67	137371.0
68	137371.0
69	137371.0
70	104285.0
71	71199.0
72	71199.0
73	71199.0
74	56153.5
75	41108.0
76	41108.0
77	41108.0
78	33061.5
79	25015.0
80	25015.0
81	25015.0
82	18080.0
83	11145.0
84	11145.0
85	11145.0
86	8096.5
87	5048.0
88	5048.0
89	5048.0
90	3458.0
91	1868.0
92	1868.0
93	1868.0
94	1071.5
95	275.0
96	275.0
97	275.0
98	166.5
99	58.0
100	58.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.3295923269147525
2	0.01066380142283413
3	0.0
4	0.0
5	0.0489978932674772
6	0.0023500794604824032
7	0.0033355966535879265
8	3.5377540265326497E-4
9	0.00924869981222107
10	0.015237611985708483
11	0.005862563815396962
12	0.00912235145413062
13	0.017385534073246164
14	0.0030828999374070236
15	0.020215737294472284
16	0.010335295691798956
17	0.0055845974275979685
18	2.0215737294472286E-4
19	0.004396922861547722
20	0.004321113846693451
21	0.00212265241591959
22	0.0
23	0.0032597876387336563
24	0.003840990085949734
25	0.002274270445628132
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	3957313.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	60.07031849471126
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.68350520640486	53.27246402976124
2	7.002057744821556	8.412316776995809
3	1.64061853780407	2.9565743428265385
4	0.725930148761302	1.7442742096401815
5	0.4149723851828299	1.2463761672221294
6	0.2653768097751921	0.9564761690583715
7	0.18886523046987366	0.794163618283165
8	0.15156735389245451	0.7283759377376285
9	0.10638308791509829	0.575141937615974
>10	0.6780922933068735	7.470983113754813
>50	0.06242280972199529	2.6284277201004413
>100	0.06350516259853121	8.268287414622415
>500	0.010529377510394695	4.374101581035254
>1k	0.0060892785224493364	6.158903257901057
>5k	4.228665640589817E-5	0.15764313131458368
>10k+	4.228665640589817E-5	0.25549059213053216
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	10058	0.2541623571347528	No Hit
TATCAACGCAGAGTACTTTTTTTTT	6206	0.15682358206186875	No Hit
GTATCAACGCAGAGTACATGGGGAA	4181	0.10565249703523578	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	2.5269671618090357E-5	0.0
5	0.0	0.0	0.0	5.0539343236180714E-5	0.0
6	0.0	0.0	0.0	7.580901485427107E-5	2.5269671618090357E-5
7	0.0	0.0	0.0	7.580901485427107E-5	2.5269671618090357E-5
8	0.0	0.0	0.0	7.580901485427107E-5	2.5269671618090357E-5
9	0.0	0.0	0.0	1.2634835809045177E-4	2.5269671618090357E-5
10	0.0	0.0	0.0	1.2634835809045177E-4	2.5269671618090357E-5
11	2.5269671618090357E-5	0.0	0.0	1.5161802970854214E-4	2.5269671618090357E-5
12	2.5269671618090357E-5	0.0	0.0	1.5161802970854214E-4	1.0107868647236143E-4
13	2.5269671618090357E-5	0.0	0.0	2.0215737294472286E-4	1.0107868647236143E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1790	0.0	16.29234	1
GTATCAA	5995	0.0	13.512782	1
CGCATCG	460	0.0	12.388808	13
GCATCGC	465	0.0	12.255129	14
CGTCGTA	530	0.0	12.007013	10
ATCGCCA	475	0.0	11.996066	16
ACCGTCG	540	0.0	11.784215	8
CGAACGA	210	0.0	11.758049	16
CCGTCGT	545	0.0	11.676544	9
TCCAACG	555	0.0	11.6355295	18
CGCCAGT	485	0.0	11.55262	18
CATCGCC	515	0.0	11.4341545	15
CGTCTTA	325	0.0	11.39727	15
ATACCGT	555	0.0	11.2937355	6
CAACGCT	60	0.0058728196	11.084743	4
CCAACGA	370	0.0	11.036642	19
TCGCCAG	505	0.0	10.907038	17
TACCGTC	590	0.0	10.785552	7
TCGCGTA	240	0.0	10.685347	9
CGAGCCG	640	0.0	10.68494	15
>>END_MODULE