FastQCFastQC Report
Thu 2 Feb 2017
SRR4050216_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4050216_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3924288
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNN130980.3337675522285826No Hit
GTATCAACGCAGAGTACTTTTTTTT59710.15215498964398128No Hit
GNNNNNNNNNNNNNNNNNNNNNNNN57820.1473388293621671No Hit
GTATCAACGCAGAGTACATGGGGAA42970.1094975700050557No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA17400.016.6105631
GTATCAA59800.013.4184161
TCCAACG5500.011.74504918
CGTCGTA4300.011.70829810
CGAACGA3250.011.39960816
CGGTTCT5500.011.39931512
CCAACGA3600.011.34668519
CCGTCCG600.0058821111.0823829
CGACCAT5700.010.83240310
CCGACCA5700.010.8324039
CCGTCGT4650.010.8270289
CGCGCTA1701.4006218E-1010.61728216
TGTACCG2550.010.4314165
TCGGCGT2550.010.43074513
GTCGTAG4850.010.38055311
ACCGTCG5000.010.259128
TACCGTC5650.010.2558897
CGCATCG4300.010.1621813
GGTTCTA6250.010.03139713
GTATAGG3800.010.007751
Produced by FastQC (version 0.11.5)