##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4050216_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3924288
Sequences flagged as poor quality	0
Sequence length	25
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.94025413017597	31.0	30.0	34.0	26.0	34.0
2	30.003721694228354	31.0	31.0	34.0	26.0	34.0
3	30.14861829712804	31.0	31.0	34.0	26.0	34.0
4	33.60300773031949	37.0	35.0	37.0	28.0	37.0
5	33.62691499706443	37.0	35.0	37.0	28.0	37.0
6	33.70395470464961	37.0	35.0	37.0	29.0	37.0
7	33.65037683268914	37.0	35.0	37.0	29.0	37.0
8	33.594334819462794	37.0	35.0	37.0	28.0	37.0
9	35.10985738049807	39.0	35.0	39.0	28.0	39.0
10	34.97512771743562	39.0	35.0	39.0	28.0	39.0
11	35.019791106055415	39.0	35.0	39.0	28.0	39.0
12	34.93553837027252	39.0	35.0	39.0	27.0	39.0
13	34.948923218683234	39.0	35.0	39.0	27.0	39.0
14	35.994298583590194	40.0	36.0	41.0	27.0	41.0
15	35.980444095846174	40.0	36.0	41.0	27.0	41.0
16	35.9866357922762	40.0	36.0	41.0	27.0	41.0
17	35.95903639080516	40.0	36.0	41.0	27.0	41.0
18	35.90240751952966	40.0	36.0	41.0	27.0	41.0
19	35.94953504941533	40.0	36.0	41.0	27.0	41.0
20	35.92297481734266	40.0	36.0	41.0	27.0	41.0
21	35.83731545696952	40.0	36.0	41.0	27.0	41.0
22	35.74418493240048	40.0	36.0	41.0	27.0	41.0
23	35.67342330634245	40.0	36.0	41.0	27.0	41.0
24	35.61089400166349	40.0	36.0	41.0	27.0	41.0
25	35.276225649085895	39.0	36.0	41.0	25.0	41.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	1.1674504363995837
1101	2	1.28802080194842
1101	3	1.3015721028088016
1101	4	1.3296432470287556
1101	5	1.3022241818229645
1101	6	1.219996854257161
1101	7	1.191265315691524
1101	8	1.300136190969745
1101	9	1.4406308607151672
1101	10	1.5065086990677727
1101	11	1.4541108782312122
1101	12	1.4822143629341227
1101	13	1.48003456812895
1101	14	1.679655531654113
1101	15	1.6566534019818278
1101	16	1.6604402801126312
1101	17	1.6521205887896997
1101	18	1.7401846528591562
1101	19	1.7752853528628592
1101	20	1.7702964014136455
1101	21	1.6865685936704864
1101	22	1.7452783355161827
1101	23	1.8774119120270214
1101	24	1.9181930872130266
1101	25	1.7618449620698797
1102	1	1.1965897039665734
1102	2	1.167090798169916
1102	3	1.1718417664310792
1102	4	1.2627360961418361
1102	5	1.1756096723615457
1102	6	1.1157876730100469
1102	7	1.1331083568177647
1102	8	1.1707267470123526
1102	9	1.333429591308004
1102	10	1.4013945315556242
1102	11	1.3731766418248696
1102	12	1.31592299928721
1102	13	1.342366002021059
1102	14	1.615539759973089
1102	15	1.5598855162061867
1102	16	1.597316067843046
1102	17	1.5016996369447142
1102	18	1.5748524478356174
1102	19	1.6020486750388088
1102	20	1.5089360675213186
1102	21	1.5242851781861262
1102	22	1.669886759713755
1102	23	1.7022354019532955
1102	24	1.7259975500697209
1102	25	1.7065255645207458
1103	1	1.1172748263898775
1103	2	1.1486745684967339
1103	3	1.1344497817555919
1103	4	1.2572634181120392
1103	5	1.236309052972672
1103	6	1.194682515835673
1103	7	1.1199072894508646
1103	8	1.140866454676086
1103	9	1.2391756757334917
1103	10	1.3488619144313816
1103	11	1.3343539409142409
1103	12	1.2293929229338332
1103	13	1.255307000736785
1103	14	1.463096182713592
1103	15	1.5077028759406161
1103	16	1.5163771462031619
1103	17	1.5577330994006147
1103	18	1.5968048122894913
1103	19	1.5447415274492045
1103	20	1.5543359784293571
1103	21	1.6049055917083024
1103	22	1.6026929395353804
1103	23	1.636012840498502
1103	24	1.636717536454107
1103	25	1.6286211186927417
1104	1	0.8141659677048132
1104	2	0.866543294545508
1104	3	0.9508897864854653
1104	4	1.0022868759487196
1104	5	0.8982116608978714
1104	6	0.8771535078931478
1104	7	0.864929847945568
1104	8	0.891895418484232
1104	9	1.0095330309352732
1104	10	1.0812999472706863
1104	11	1.063192773834821
1104	12	1.0321523706046207
1104	13	1.0017888248687896
1104	14	1.259893226210103
1104	15	1.067127929942771
1104	16	1.1814243866389091
1104	17	1.183567363997014
1104	18	1.110418085244774
1104	19	1.1207584084312856
1104	20	1.104676812385847
1104	21	1.234866337096264
1104	22	1.286804198131172
1104	23	1.2400441575848404
1104	24	1.2635403444329327
1104	25	1.2383299150900484
1105	1	0.7022081171468635
1105	2	0.8519469279664307
1105	3	0.806463637437993
1105	4	0.8778936992942121
1105	5	0.8129384653380711
1105	6	0.7750752988211289
1105	7	0.7459406708176601
1105	8	0.7509222307677419
1105	9	0.8942001436237419
1105	10	0.9387357252789457
1105	11	0.8439636703653477
1105	12	0.9181211923837367
1105	13	0.9952782454951077
1105	14	1.1262523217307603
1105	15	1.0884355348603307
1105	16	1.1462550417862971
1105	17	1.0334770642848525
1105	18	1.1264908873935084
1105	19	1.1449196673060058
1105	20	1.1609457937895797
1105	21	1.2044207724983806
1105	22	1.2876014949491932
1105	23	1.2824390048507581
1105	24	1.1015879028321933
1105	25	1.1322345860010827
1106	1	0.647892693677651
1106	2	0.7670341489139822
1106	3	0.7960099314001035
1106	4	0.8026469516569747
1106	5	0.7928881305354452
1106	6	0.7493644656614009
1106	7	0.6790074211958483
1106	8	0.6177163598975213
1106	9	0.7705815670133092
1106	10	0.847343345059592
1106	11	0.8883950653604415
1106	12	0.9325106651472623
1106	13	0.8827055098505312
1106	14	1.05025766203741
1106	15	1.1149329515966997
1106	16	1.0749847629531146
1106	17	1.0650769093320704
1106	18	1.0810275446915298
1106	19	1.0739727927013263
1106	20	1.110039646721063
1106	21	1.15449896110011
1106	22	1.1648657867278729
1106	23	1.1723901314436773
1106	24	1.1129968802862749
1106	25	1.174058017293575
1107	1	0.5214454498696419
1107	2	0.6254343076967714
1107	3	0.6315789616655998
1107	4	0.6749385993742294
1107	5	0.5865826753411625
1107	6	0.6030155253708784
1107	7	0.601928722296428
1107	8	0.4936537348495378
1107	9	0.5516849039800888
1107	10	0.6562471538995212
1107	11	0.5983745811019929
1107	12	0.5759850383199421
1107	13	0.6164737619524985
1107	14	0.7033638212927116
1107	15	0.6938801670713914
1107	16	0.7197739180653642
1107	17	0.6750161481801555
1107	18	0.717143945494854
1107	19	0.7194790566881721
1107	20	0.7845276928238647
1107	21	0.6927289688968585
1107	22	0.78215704068473
1107	23	0.6167888680394782
1107	24	0.6377957065025228
1107	25	0.6047027469051471
1108	1	0.5969776247602745
1108	2	0.6695895487946046
1108	3	0.6413483506948339
1108	4	0.6962886939115052
1108	5	0.7092484044113192
1108	6	0.633886112612899
1108	7	0.6523449196093907
1108	8	0.6996816250129996
1108	9	0.7359377262122351
1108	10	0.8195574694607402
1108	11	0.8402510405420571
1108	12	0.7155447234474863
1108	13	0.8332972316372533
1108	14	0.7867327588536739
1108	15	0.8953270540065859
1108	16	0.8514938411024673
1108	17	0.9066898579295994
1108	18	0.8875744400436858
1108	19	0.9374590250054098
1108	20	0.9171341666833683
1108	21	0.9134329105792389
1108	22	0.9064983347012188
1108	23	0.8730822624276726
1108	24	0.8279001196493141
1108	25	0.8172265478591854
1109	1	0.4500555646177098
1109	2	0.5950439225849067
1109	3	0.4467543795430089
1109	4	0.5695983375794569
1109	5	0.6310836668215742
1109	6	0.4218962236367503
1109	7	0.49603422020635435
1109	8	0.4775486324331837
1109	9	0.5612234398848628
1109	10	0.5765598684685216
1109	11	0.4891365104514591
1109	12	0.5161388963047955
1109	13	0.5114133667885099
1109	14	0.5377898887133838
1109	15	0.5102917770048734
1109	16	0.5137945496466259
1109	17	0.5200381475100144
1109	18	0.4667509237591858
1109	19	0.4316649276803659
1109	20	0.4512790427203228
1109	21	0.5024550895992945
1109	22	0.621249657588784
1109	23	0.4614381696088188
1109	24	0.4322077119295713
1109	25	0.4418011424097017
1110	1	-0.040858814163119206
1110	2	0.17476553223145785
1110	3	0.08863331211022896
1110	4	0.06791959923806701
1110	5	0.16415617779735214
1110	6	0.09231826017548173
1110	7	-0.09603640920671808
1110	8	-0.01754969439223686
1110	9	0.08807870117929895
1110	10	0.13664292024184022
1110	11	0.1739231655185307
1110	12	0.022108811533314565
1110	13	-0.02609953102037821
1110	14	0.05665558964606987
1110	15	-0.0496193039832491
1110	16	0.022277767321170927
1110	17	0.044945080967437434
1110	18	0.11605646526484037
1110	19	0.08824483339991218
1110	20	0.09640670573851651
1110	21	0.09847902388622742
1110	22	0.05656891629354277
1110	23	0.008791842702471797
1110	24	-0.03847480976909878
1110	25	0.043219395591158616
1111	1	-0.013860204500275586
1111	2	0.06556172693910867
1111	3	-0.12326521594586737
1111	4	-0.028385050835233017
1111	5	-0.002013292212168949
1111	6	-0.07793456961400125
1111	7	-0.16670482890719285
1111	8	-0.1354404622512675
1111	9	-0.08564128189485842
1111	10	-0.19777529166367458
1111	11	-0.11288733303824472
1111	12	-0.12043928355365807
1111	13	-0.09907031882772799
1111	14	-0.1689721400240245
1111	15	-0.10538580290271682
1111	16	-0.17528517565989432
1111	17	-0.14116892667941983
1111	18	-0.15854607965664513
1111	19	-0.20762779207046123
1111	20	-0.14098410745759793
1111	21	0.008762796233625636
1111	22	-0.0041463139432664775
1111	23	-0.0940740275428027
1111	24	-0.01783548136238977
1111	25	-0.07782059927063756
1112	1	-0.31322184539711984
1112	2	-0.2566155834063224
1112	3	-0.3449374856961924
1112	4	-0.28268111167120225
1112	5	-0.214264423729837
1112	6	-0.32250045054084353
1112	7	-0.43006284197088007
1112	8	-0.4033637893928699
1112	9	-0.48471491993129234
1112	10	-0.5606943238345323
1112	11	-0.467329793511432
1112	12	-0.5100498147347139
1112	13	-0.4491562182425639
1112	14	-0.5825609260080356
1112	15	-0.6538699180515337
1112	16	-0.5062077658978836
1112	17	-0.5214100990974231
1112	18	-0.615110190270677
1112	19	-0.4718355334826967
1112	20	-0.5189529787305673
1112	21	-0.4804545380066614
1112	22	-0.46408808111660704
1112	23	-0.4954712011468274
1112	24	-0.5281510418015714
1112	25	-0.580179017849332
1113	1	-0.5396613351715231
1113	2	-0.3867361119044581
1113	3	-0.5311072797786984
1113	4	-0.39545818665890664
1113	5	-0.35797937207476593
1113	6	-0.4946126861160778
1113	7	-0.49703293825312045
1113	8	-0.44806211675248164
1113	9	-0.5156169509946196
1113	10	-0.5136082671896531
1113	11	-0.4112668235205632
1113	12	-0.4550696685543727
1113	13	-0.512687035587426
1113	14	-0.6284028685198066
1113	15	-0.6272700164421465
1113	16	-0.5800304168220478
1113	17	-0.6683322299569383
1113	18	-0.5379607068213801
1113	19	-0.695419762873378
1113	20	-0.7169902617441295
1113	21	-0.7416232779040541
1113	22	-0.6711028045687115
1113	23	-0.8197814126866376
1113	24	-0.8859437538684745
1113	25	-0.8838321206675204
1114	1	-0.7617516972347467
1114	2	-0.7415810456671323
1114	3	-0.8916522109790002
1114	4	-0.7391848156871674
1114	5	-0.706422132945633
1114	6	-0.7969204441256252
1114	7	-0.695927125084232
1114	8	-0.7214521995171168
1114	9	-0.9393878236532487
1114	10	-0.8133939887964345
1114	11	-0.8555701998732843
1114	12	-1.20102805989697
1114	13	-1.0580177097476096
1114	14	-1.0743354439603152
1114	15	-1.0347394601510942
1114	16	-1.0558230653755913
1114	17	-1.3644706920210155
1114	18	-1.1288823067667195
1114	19	-1.0896748812122752
1114	20	-1.039336732930252
1114	21	-1.106160940507941
1114	22	-1.0055565708290999
1114	23	-1.2187194374391552
1114	24	-1.106326721779034
1114	25	-1.136467691521382
1115	1	-1.3225583708626765
1115	2	-1.279710683114324
1115	3	-1.4234480238841485
1115	4	-1.502293004670193
1115	5	-1.442609127880992
1115	6	-1.361779937400975
1115	7	-1.430503951282958
1115	8	-1.5270929332832992
1115	9	-2.1393678447455073
1115	10	-1.8237073368475478
1115	11	-1.7890749257404366
1115	12	-1.7565849443343566
1115	13	-1.7586811809933351
1115	14	-2.0056562563614264
1115	15	-2.036414328821749
1115	16	-2.15706772724171
1115	17	-2.0192512582289055
1115	18	-1.9067739738699458
1115	19	-1.9745796370332513
1115	20	-2.0215477393898453
1115	21	-2.116691581501769
1115	22	-2.3211813495002005
1115	23	-2.174146996758104
1115	24	-2.3510263364510564
1115	25	-2.2033321248734055
1116	1	-1.7167376499252
1116	2	-1.8244851058076321
1116	3	-1.6929499832673045
1116	4	-2.0166270261734063
1116	5	-1.5928202039421322
1116	6	-1.764486090619215
1116	7	-1.756076905286882
1116	8	-1.7569911758812573
1116	9	-2.2725184940780494
1116	10	-2.9809493802018423
1116	11	-2.4140321238233966
1116	12	-1.9730413492381231
1116	13	-2.0172241507202457
1116	14	-2.2335298254885814
1116	15	-2.9495369674667202
1116	16	-2.476693609341993
1116	17	-2.340908199304117
1116	18	-2.462796960044507
1116	19	-2.2877746720635983
1116	20	-2.331268181433103
1116	21	-2.2677291409784956
1116	22	-2.3371578808392854
1116	23	-2.4484749327001367
1116	24	-2.209792927704335
1116	25	-2.427046328923538
1201	1	1.1327887245764074
1201	2	1.1752499995197958
1201	3	1.1076616873712126
1201	4	1.0974466901250821
1201	5	1.0688618787989057
1201	6	1.0180419257478306
1201	7	0.9787223094189628
1201	8	1.1068008575671797
1201	9	1.2622404656401116
1201	10	1.3002535347177684
1201	11	1.2532504582867503
1201	12	1.2615461747933097
1201	13	1.2766194374809103
1201	14	1.5382852923712278
1201	15	1.5148329980100925
1201	16	1.4376168110993959
1201	17	1.458257515945256
1201	18	1.4987206901528936
1201	19	1.537755783312896
1201	20	1.6273377994918619
1201	21	1.563137730370471
1201	22	1.6338915024680105
1201	23	1.6221198115822943
1201	24	1.6347758705086832
1201	25	1.7052883446899543
1202	1	0.8371293609727068
1202	2	0.9697792190601433
1202	3	0.846143235639996
1202	4	0.9401535166353909
1202	5	0.8636203998250451
1202	6	0.8254943455108901
1202	7	0.8453565208862415
1202	8	0.869974795999191
1202	9	0.963741736551988
1202	10	1.1088930487729698
1202	11	0.9739884732290065
1202	12	1.0666793554400087
1202	13	0.9513713931531953
1202	14	1.2030718869304735
1202	15	1.184111086334532
1202	16	1.1209792555032578
1202	17	1.184811385431054
1202	18	1.3127902691842337
1202	19	1.2523020864927688
1202	20	1.2544267010141255
1202	21	1.2680790952152137
1202	22	1.3191903261063729
1202	23	1.3556519776717408
1202	24	1.2856203773768655
1202	25	1.3303074591017534
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2211	9	-0.3860810665624683
2211	10	-0.5014888528033339
2211	11	-0.46203834559182155
2211	12	-0.5737275166636522
2211	13	-0.5031043237567303
2211	14	-0.6374676765545928
2211	15	-0.6288069585587834
2211	16	-0.6279333765305992
2211	17	-0.6540389801535582
2211	18	-0.8028348242876433
2211	19	-0.7862965109907947
2211	20	-0.6679042322193638
2211	21	-0.6743654379308168
2211	22	-0.7136295178819765
2211	23	-0.6965852619349633
2211	24	-0.615070463565722
2211	25	-0.5759602360907294
2212	1	-0.6171099924988823
2212	2	-0.5859223922954406
2212	3	-0.5544704894896988
2212	4	-0.6385761513012653
2212	5	-0.6380979365971555
2212	6	-0.5765753524063513
2212	7	-0.5756042534018562
2212	8	-0.6049832325293281
2212	9	-0.7039252893269534
2212	10	-0.6959228336881154
2212	11	-0.7531241579360355
2212	12	-0.7218596929024486
2212	13	-0.6535995589651051
2212	14	-0.7821994033304875
2212	15	-0.8883489183088287
2212	16	-0.9524033834052048
2212	17	-0.8832214285877953
2212	18	-0.9010702025692936
2212	19	-0.9663465775104498
2212	20	-0.9369910557029897
2212	21	-0.8658391994256363
2212	22	-0.8130630889824175
2212	23	-0.8181710876928179
2212	24	-0.8330808264539584
2212	25	-0.9533165767174836
2213	1	-0.8663604757849477
2213	2	-0.7061300961984287
2213	3	-0.72838687675603
2213	4	-0.6921347313321888
2213	5	-0.6064947076800422
2213	6	-0.7483673164316755
2213	7	-0.807309645913513
2213	8	-0.7880550786727696
2213	9	-0.7928091276133742
2213	10	-0.9506711653967983
2213	11	-0.96171059296789
2213	12	-0.9636913162058818
2213	13	-0.9799962665846067
2213	14	-1.0787718120669965
2213	15	-1.0909038881268387
2213	16	-1.1930697559834087
2213	17	-1.1767845932700212
2213	18	-1.1199447044506599
2213	19	-1.083972881853967
2213	20	-1.2204466997349428
2213	21	-1.130326048684914
2213	22	-1.1081508857659301
2213	23	-1.0941502080637306
2213	24	-1.1199032293463205
2213	25	-1.0669585097621948
2214	1	-0.9039300521454159
2214	2	-0.9119939553323739
2214	3	-0.9327127627664389
2214	4	-0.9485603299769494
2214	5	-0.9839899055499401
2214	6	-1.0221289484763432
2214	7	-1.0434659879034598
2214	8	-1.097013347111215
2214	9	-1.176390742871284
2214	10	-1.6326354466947208
2214	11	-1.5188426418704353
2214	12	-1.315853182968148
2214	13	-1.3314176123126273
2214	14	-1.4852044590047981
2214	15	-1.508612947329297
2214	16	-1.5512311719536314
2214	17	-1.5362871836549843
2214	18	-1.4521720041479043
2214	19	-1.5408288942104704
2214	20	-1.603998641437542
2214	21	-1.5865107929456528
2214	22	-1.4594172305466344
2214	23	-1.7012961229288734
2214	24	-1.5465344742953917
2214	25	-1.360747068087342
2215	1	-1.669704160706086
2215	2	-1.3043677863785383
2215	3	-1.4795966749490503
2215	4	-2.0932374817445556
2215	5	-1.7811832924910114
2215	6	-1.4941616999619924
2215	7	-1.5151993052994968
2215	8	-1.6792662013836903
2215	9	-1.6972772507301457
2215	10	-1.8213602939480467
2215	11	-1.799817669659788
2215	12	-1.8327781351323083
2215	13	-1.9229760539276413
2215	14	-2.0145493482695045
2215	15	-1.9778505595615385
2215	16	-2.046731035653181
2215	17	-2.071747123390068
2215	18	-2.388251799455908
2215	19	-2.799068014416136
2215	20	-2.3939132622108517
2215	21	-2.2347892560202496
2215	22	-3.1044464373494804
2215	23	-2.4000096714894283
2215	24	-2.3981822199278326
2215	25	-2.3206971705370094
2216	1	-3.2122546940190766
2216	2	-5.987748976703948
2216	3	-3.701882895474917
2216	4	-6.201773038750986
2216	5	-3.9355996741034787
2216	6	-2.9115752514548454
2216	7	-2.708733837877862
2216	8	-2.5712859584649195
2216	9	-2.923690461767748
2216	10	-2.8267484007251156
2216	11	-2.871427240031913
2216	12	-2.976518076815019
2216	13	-2.8909329330169484
2216	14	-3.513794486933115
2216	15	-3.320562137684391
2216	16	-3.387209873733667
2216	17	-3.2001627509274684
2216	18	-3.2225927801830494
2216	19	-3.252511017626233
2216	20	-3.5647938643850168
2216	21	-3.723545583793033
2216	22	-3.69068610683766
2216	23	-3.5672369906085564
2216	24	-3.5824136172679957
2216	25	-3.826477812657874
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	143892.0
3	9.0
4	1033.0
5	6126.0
6	7475.0
7	7084.0
8	6077.0
9	5838.0
10	5493.0
11	5423.0
12	5103.0
13	5058.0
14	5061.0
15	5094.0
16	5466.0
17	5747.0
18	6662.0
19	7753.0
20	10119.0
21	12990.0
22	16201.0
23	19627.0
24	23116.0
25	26606.0
26	29849.0
27	33600.0
28	38924.0
29	46431.0
30	58497.0
31	74948.0
32	96389.0
33	126940.0
34	172795.0
35	238739.0
36	352463.0
37	614967.0
38	1696693.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.04972214546683	18.79235618597321	13.00742992232354	25.150491746236426
2	15.12635208612706	20.617321585188677	40.32114948297797	23.935176845706295
3	18.23627930945633	25.533398485419312	29.62850992320335	26.601812281921017
4	12.162522181971992	17.267263182264074	38.346694276602065	32.22352035916187
5	13.855059696385618	37.22598995789565	34.378048958916494	14.540901386802238
6	30.85509683424197	37.77766255341355	18.364071286347976	13.003169325996508
7	27.532555421171118	31.663409177672513	22.39937234395695	18.404663057199418
8	25.677508688180943	34.741736794196	20.78242659135284	18.798327926270215
9	26.90885523613963	15.111165297741271	20.047715605749485	37.93226386036961
10	15.59921144265391	27.87443961841052	32.68922334903826	23.83712558989731
11	34.73890453695929	22.4144768708742	24.285408825340582	18.561209766825925
12	23.8743596345338	24.760250403850264	30.464774990008024	20.900614971607908
13	28.605719988848964	21.30451211391718	25.95390904923039	24.135858848003465
14	22.598604052703838	21.801407653783713	26.487519754697743	29.112468538814706
15	23.792940304258547	29.32913560147424	23.298284634047477	23.579639460219738
16	23.68240013717886	27.30719234098393	26.01379900216755	22.99660851966966
17	22.519735452139585	27.327302118575215	26.58049838771413	23.572464041571074
18	22.771462583296344	26.671439966174436	28.377017410049447	22.180080040479773
19	24.71782093191683	25.800839149779996	26.54065984396425	22.940680074338925
20	24.791135333009024	26.18288188958186	26.35554420332361	22.67043857408551
21	25.802990245494005	25.526340041272917	25.35203783410383	23.31863187912925
22	24.78469577576013	26.22886301193319	26.083735154110745	22.90270605819594
23	23.61102197834356	25.92131764299991	26.40689584712039	24.060764531536144
24	23.750421001614182	26.493785605441396	26.59862527570472	23.157168117239706
25	23.827498755573608	26.016148919396336	26.681477415875104	23.47487490915495
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	18030.0
1	18030.0
2	14279.0
3	10528.0
4	10528.0
5	10528.0
6	7327.0
7	4126.0
8	4126.0
9	4126.0
10	5913.5
11	7701.0
12	7701.0
13	7701.0
14	12715.0
15	17729.0
16	17729.0
17	17729.0
18	30539.5
19	43350.0
20	43350.0
21	43350.0
22	68568.5
23	93787.0
24	93787.0
25	93787.0
26	136265.0
27	178743.0
28	178743.0
29	178743.0
30	224328.0
31	269913.0
32	269913.0
33	269913.0
34	302459.0
35	335005.0
36	335005.0
37	335005.0
38	358935.5
39	382866.0
40	382866.0
41	382866.0
42	412103.0
43	441340.0
44	441340.0
45	441340.0
46	463363.0
47	485386.0
48	485386.0
49	485386.0
50	478171.0
51	470956.0
52	470956.0
53	470956.0
54	419850.5
55	368745.0
56	368745.0
57	368745.0
58	328536.5
59	288328.0
60	288328.0
61	288328.0
62	250946.0
63	213564.0
64	213564.0
65	213564.0
66	175520.5
67	137477.0
68	137477.0
69	137477.0
70	104794.5
71	72112.0
72	72112.0
73	72112.0
74	56857.0
75	41602.0
76	41602.0
77	41602.0
78	33027.5
79	24453.0
80	24453.0
81	24453.0
82	17754.5
83	11056.0
84	11056.0
85	11056.0
86	8138.0
87	5220.0
88	5220.0
89	5220.0
90	3554.0
91	1888.0
92	1888.0
93	1888.0
94	1097.0
95	306.0
96	306.0
97	306.0
98	191.5
99	77.0
100	77.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.40180537208278294
2	0.6921510347864377
3	0.7074913971655495
4	0.7162063538659752
5	0.7260170507363374
6	0.7264247680088719
7	0.743090211523721
8	0.7330246913580246
9	0.7208441378410555
10	0.715008684377905
11	0.726985379258607
12	0.7303490467570168
13	0.730986104995352
14	0.7392933444232431
15	0.7351906893683644
16	0.7299158471549488
17	0.736108053231567
18	0.7392168974346429
19	0.7429373175465205
20	0.7344771841414289
21	0.7327443857331573
22	0.7415867540812499
23	0.7320053981766884
24	0.7342988078346951
25	0.7369234877766362
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	3924288.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	60.50521650977673
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.00316407361692	53.85155712329372
2	6.747835699058949	8.165585198879247
3	1.6233639564053468	2.9466596296941976
4	0.7285626169104458	1.7632735550838414
5	0.39660336602227664	1.1998286264842035
6	0.2551919869922827	0.9264267854716914
7	0.185917685704197	0.7874292878570343
8	0.14470932997846506	0.7004535473065402
9	0.10866802505982062	0.591748414554086
>10	0.6671046723947097	7.37311773280265
>50	0.060694882865944697	2.5885016036509634
>100	0.06203096430532712	8.117334766284017
>500	0.010259746068916479	4.327113173437736
>1k	0.005765807682341913	6.023503224357286
>5k	8.479128944620462E-5	0.30148298013742947
>10k+	4.239564472310231E-5	0.3359843507053562
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
NNNNNNNNNNNNNNNNNNNNNNNNN	13098	0.3337675522285826	No Hit
GTATCAACGCAGAGTACTTTTTTTT	5971	0.15215498964398128	No Hit
GNNNNNNNNNNNNNNNNNNNNNNNN	5782	0.1473388293621671	No Hit
GTATCAACGCAGAGTACATGGGGAA	4297	0.1094975700050557	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	2.5482329533408354E-5	0.0
5	0.0	0.0	0.0	5.096465906681671E-5	0.0
6	0.0	0.0	0.0	5.096465906681671E-5	0.0
7	0.0	0.0	0.0	7.644698860022506E-5	0.0
8	0.0	0.0	0.0	7.644698860022506E-5	0.0
9	0.0	0.0	0.0	1.2741164766704176E-4	0.0
10	0.0	0.0	0.0	1.5289397720045012E-4	0.0
11	0.0	0.0	0.0	2.0385863626726683E-4	0.0
12	0.0	0.0	0.0	2.293409658006752E-4	1.0192931813363342E-4
13	0.0	0.0	0.0	2.293409658006752E-4	1.7837630673385847E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1740	0.0	16.610563	1
GTATCAA	5980	0.0	13.418416	1
TCCAACG	550	0.0	11.745049	18
CGTCGTA	430	0.0	11.708298	10
CGAACGA	325	0.0	11.399608	16
CGGTTCT	550	0.0	11.399315	12
CCAACGA	360	0.0	11.346685	19
CCGTCCG	60	0.00588211	11.082382	9
CGACCAT	570	0.0	10.832403	10
CCGACCA	570	0.0	10.832403	9
CCGTCGT	465	0.0	10.827028	9
CGCGCTA	170	1.4006218E-10	10.617282	16
TGTACCG	255	0.0	10.431416	5
TCGGCGT	255	0.0	10.430745	13
GTCGTAG	485	0.0	10.380553	11
ACCGTCG	500	0.0	10.25912	8
TACCGTC	565	0.0	10.255889	7
CGCATCG	430	0.0	10.16218	13
GGTTCTA	625	0.0	10.031397	13
GTATAGG	380	0.0	10.00775	1
>>END_MODULE