FastQCFastQC Report
Thu 2 Feb 2017
SRR4050216_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4050216_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3924288
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT102240.260531337149567No Hit
TATCAACGCAGAGTACTTTTTTTTT61670.1571495262325293No Hit
GTATCAACGCAGAGTACATGGGGAA41570.10593004387037853No Hit
GGTATCAACGCAGAGTACTTTTTTT39250.10001814341862778No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA17900.016.3652171
GTATCAA62100.013.5215831
CGAGCCG4800.011.86616715
CGAACGT2650.011.8459944
GCGTTAT2250.011.0262371
CGAACGA3100.011.02405316
TCGAACG2850.011.0146973
TAAACCG951.341548E-511.0146965
AACCGCG2750.010.70126157
ATTCGAA3050.010.6369111
CGTCGTA5150.010.5070110
CAAGACG4700.010.1198854
TCCAACG4800.010.08611418
CCAACGA3300.010.06864819
CCGATAA3400.010.0515989
CGCATCG4450.010.02662813
AGAACCG3800.010.013365
GTATTAG7450.09.9902141
AGGCCCG5900.09.9758810
CGGTCCA5450.09.92864210
Produced by FastQC (version 0.11.5)